1 Final Lab Report: Water Quality & Contamination Student: Monica McNeal SCI207: Dependence of Man of on the Environment Instructor: Jeffrey Sigler Date: August 5, 2013 2 A Study of the groundwater contamination, water treatment and water quality Abstract In this experiment we explore the effects of groundwater contamination, study the water treatment process and test the quality of tap water versus bottled water. Groundwater is rain water that infiltrates the soil and underlying rocks, or water from lakes that is absorbed in the soil or bedrock. When harmful substances are absorbed in the groundwater, the contamination is not only harmful for humans, but also causes problems to the flora and fauna. In the first experiment we study the effects of groundwater contamination. Two main types of contaminants, parabens and ammonium, have had the most potent effect on greywater, due to a fact that parabens produced its grey color, and ammonium content influenced its purgent odor (citation). By mixing water with common products we have in our household we were able to show the chemical poperties of the water were changed by vegetable oil, vinegar and laundry detergent. Imagine that happening on a global scale, and that will give us a picture of massive groundwater contamination that is detrimental to the living organisms. In the second experiment we studied the process of water filtration. We created a home filtration system, similar in concept to the ones used municipal wastewater 3 treatment plans. This experiment made us aware that succesfull wastewater treatment will eliminate the ammonia, chloride, phosphate and iron from tap water. The test strips helped us identify the amounts of the chemical elements that are still present in the drinking water. The third experiment examined the levels of various chemicals in bottled water and tap water. The bottled water industry is a multi-million dollar industry in the United States (citation??). This experiment helped us properly understand the fact that bottled water is not necessarily free of chemicals and may not be more beneficial compared to the treated tapwater. Introduction Water is a basic living necessity that needs to be protected. According to the EPA, "40% of people living in the United States use groundwater for drinking, cooking, cleaning, showering, and all other types of activities. 97% of people living in rural areas of the nation use groundwater, and 30-40% of water used agriculturally (for farming) is from the ground" (1) In other words, water is used for many important things such as drinking, washing, cooking, and feeding animals. Wastewater contamination is highly detrimental to humans, plants and animals. Groundwater contamination is separated into biological and organic and inorganic chemicals. Pesticides are used to kill insects but can easily be absorbed in wastewater and cause liver damage and cancer in humans (2). Nitrates, found in fertilizers, can also cause serious problems. According to the 4 Michigan Environmental Education Curriculum for Groundwater Contamination, over 115 million tons of fertilizers are used in the Unites States every year. Fecal coliform can cause serious problems, especially E. Coli bacterial infestations that are highly contagiuous. A thorough waterwater treatment process is essential toward avoiding public health issues. Benzene is another form of contamination generated by leaking fuel tanks and industrial water. According to the Michigan Environmental Education Curriculum for Groundwater Contamination, too much benzene causes "cancer, nerve,lung,kidney and blood disorder(2). The wastewater treatment process is a lenghty process that includes screening, pumping, aerating, removing sludge, killing bacteria and finally disposing the wastewater residues (3). According to Suzanna Didier, "bottled water isn't always as safe as tap water". The NRDC conducted a 4 year study and decided that "roughly 22 percent of the water tested contained contaminant levels that exceeded strict water health limits. " Hormonedisrupting phtalates in plastics also have a tendency to leach into bottled water (3) You need to include hypotheses here! Materials and Methods Experiment 1 - Effects of groundwater contamination: Materials: 8-250ml beakers permanent markers 3 wooden stir sticks 100 ml graduated cylinder 5 10 ml vegetable oil 10 ml vinegar 10 ml liquid laundry detergent 240 ml soil funnel cheesecloth scissors water Procedure: 1. Using the permanent marker, label the beakers 1-8. 2. Set beakers 5-8 aside. Fill beakers 1-4 with 100 ml of water using your 100 ml graduated cylinder. 3. Add 10ml of vegetable oil to Beaker 2. Mix thoroughly with the wooden stir stick. 4. Add 10 ml vinegar to beaker 3. Mix thoroughly 5. Add 10 ml of liquid laundry detergent to beaker 4. 6. Cut cheesecloth inti five different pieces so that you have a piece 4 layers thick and big enough to line the funnel. 7. measure out 60 ml of soil using the 100 ml beaker and place it into the cheesecloth lined funnel 8. place the funnel inside beaker 5. 9. Pour the contents of Beaker 1 through the funnel so that it filters into beaker 5 for one minute. 10. Discard the cheesecloth and soil from the funnel. 6 11. record the observations in a table Experiment 2- Water treatment Materials: 1. 100 ml potting soil 2. 2-250 ml beakers 3. 2-100 ml beakers 4. 100 ml graduated cylinder 5. 40 ml sand 6. 20 ml activated charcoal 7. 60 ml gravel 8. 1 wooden stir stick 9. funnel 10. cheesecloth 11. bleach 12. stopwatch 13. water Method: 1. Add 100 ml of the soil to the 250 ml beaker. Fill to the 200 ml mark with water 2. Pour the soil solution back and forth between the two 250 ml beakers for a total of 15 times 3. Pour the mix into a clean 100 ml beaker. This sample will be used to compare to the 7 treated water at the end of the filtration process. 4. Add 10 grams of alum to the 250 ml beaker containing the contaminated water. Stir with the wooden stick and let the solution sit for 15 min. 5. Rinse out the emplty 250 ml beaker. Place the funnel into the clean 250 ml beaker. Fold a piece of cloth so that you have a piece 4 layers thick that is big enough to line the funnel. Place it inside the funnel. 6. Begin layering the funnel, starting by pouring 40 ml of sand into the cheesecloth-lined funnel, then 20 ml activated charcoal, then 40 ml gravel. Use a 100 ml beaker to measure these amounts. 7. To solidify the filter, slowly pour clean tap water through the filter until the funnel is full. Discard the rinse water from the beaker and repeat four more moves. Return the funnel to the top of the beaker and let sit for 5 minutes before emptyiong the beaker and continuing the experiment. 8. Pour about 3/4 of the contaminated water into the Funnel. Let it filter throiugh the funnel into the beaker for 5 minutes. 9. Note the smell of the filtered water, comparing to the one on step 2. 10. Remove the filter and add a few drops of bleach solution to the filtered water within the beaker. Stir the water and bleach combination slowly for 1 minute. 11. Compare the newly filtered water with the contaminated water. Experiment 3- Drinking water quality Materials 1. Dasani bottled water 2. Fiji water 8 3. Ammonia test strips 4. Chloride test strips 5. 4 in 1 test strips 6. Phosphate test strips 7. beakers 8. parafilm 9. pipettes 10. tap water Methods: 1. Label three beakers Tap water, dasani and Fiji. Pour 100 ml of each type of water into the corresponding beakers. 2. Perform the ammonia test using the test strip by reading the instructions on the package insert. 3. Read the ammonia test results after 30 seconds by comparing the color of the test strip with the color chart on the package insert. 4. Perform the chloride test using the test strip by reading the instructions on the package insert. 5. Read the chloride test results after 30 seconds by comparing the color of the test strip with the color chart on the package insert. 6. Perform the 4 in 1 test using the test strip by reading the instructions on the package insert. 7. Read the 4 in 1 test results after 20 seconds by comparing the color of the test strip 9 with the color chart on the package insert 8. Perform the phosphate test using the test strip by reading the instructions on the package insert. 9. Read the phosphate results after 45 seconds by comparing the color of the test strip with the color chart on the package insert 10. Perform the iron test using the test strip by reading the instructions on the package insert. 11. Read the iron test results after 10 seconds by comparing the color of the test strip with the color chart on the package insert Results Experiment 1- water observations Table 1: Water Observations (Smell, Color, Etc.) Beaker Observations 1 Grey wastewater with pungent odor 2 Grey wastewater with pungent odor 3 Grey wastewater with pungent odor 4 Grey wastewater with pungent odor 5 Grey wastewater with pungent odor 10 Expriment 2We compared the newly filtered water with the contaminated water. The color of the contaminated water was gray and had a pungent smell. It was also fuzzy and had reduced visibility. The color of the newly filtered water was clear and had a clean smell. The water was clear and could not see any visible sediments. EXperiment 3- Ammonia Test results Water sample Test results Tap Water yellowPut the actual numbers in your tables (be quantitative) ?? Dasani Yellow Put the numbers here! Fiji yellow Chloride test results: Water sample Test Results Tap Water light violet 11 Dasani violet Fiji light violet 4 in 1 test strip Water Sample Ph Total Alkalinity Total Chlorine Total Hardness Tap Water light red green yellow light brown Dasani light red green light green light brown Fiji light red green yellow light brown Phosphate Test results: Water Sample Test results Tap Water yellow Dasani yellow Fiji yellow Iron Test results: Water Sample Test Results 12 Tap Water light gray Dasani light gray Fiji light gray You need to include your quantitative results (numbers) Discussion: Experiment 1: Greywater from the five beakers is highly contaminated with both macro- and micropollutants, what is shown by the observations (grey color and purgent odor). So, the hypothesis was confirmed: grey color indicated parabens, while purgent odor must be connected with the ammonium content presumably from urine contamination. Two main types of contaminants, parabens and ammonium, have had the most potent effect on greywater, due to a fact that parabens produced its grey color, and ammonium content influenced its purgent odor. Experiment 2: Our household- made filtering process was succesfull and the hypothesis is proven. We were able to filter the water using cheesecloth as shown in the color, smell and visibility of the post filtered water. Experiment 3: We could find some deficiencies with the Dasani water as compared to the TapWater and Fiji. The Tap Water and Fiji water had very similar results using the color test strips. However, Dasani has higher chloride amounts which supports our hypothesis that not 13 all bottled waters are equal when it comes to the filtration process. Respond to all of the postlab questions here. Conclusion: In this experiment we were able to show that groundwater highly contaminated with both macro- and micro-pollutants, what is shown by the observations (grey color and purgent odor). In return, we have in place filtratiuon systems which makes our tap water drinkable and safe to feed animals in farms. We were able to filtrate the water and as a next step to our experiment would be to use chlorine, phosphate, iron and 4 in 1 test strips to detect how succesful was our home filtration. The test strips are a more reliable testing source than color, smell and visibility. Finally, not all bottled waters are equal in terms of the quality of the water that it is offered. We were able to show that tap water had lower levels of chloride than Dasani bottled water. However, a possible flaw with the chlorine testing is the fact that the color changes are very minutious and depends on the vision capacity of the technician that performs the test. In the future would be highly recommended to use some numerical test methods to quatify the amounts of chlorine, iron, phosphate and pH present in tap water versus bottled water. References: 1.Matthew Babcock/Michigan Tech (n.d.). Groudwater Contamination-Michigan Environmental Education Curriculum. Retrieved July 23,2013, from 14 http://techalive.mtu.edu/meec/module04/title.htm - See more at: http://reffor.us/index.php#sthash.7i1CfjJR.dpuf 2. Howard Pearlman/U.S Geological Survey (n.d.). A visit to a wastewater-treatment plant:. Retrieved July 23,2013, from http://ga.water.usgs.gov/edu/wwvisit.html 3. Didier, S. (n.d.). Water Bottle Pollution Facts. SF Gate, Retrieved from http://homeguides.sfgate.com/water-bottle-pollution-79179.html. 15