Constant Cell Disruption System
Which pressure for your use ?
 900 bar
900 to 1300 bar
Up to 1300 bar
1800 to 2200 bar
DNA
Intracellular protein
Inclusion bodies
Intramembranar protein
How to use the cell D ?
-
Switch on the cooling machin. Set point @ 4°C.
Clean the vessel with the buffer of your sample.
Put your sample in the 200 ml reservoir. Advise : Don’t forget to put a lid on
the metal beaker.
Set the pressure.
Set the timer on the maximum and press stop when it’s finished.
To collect your sample, a beaker has to be positioned underneath the outlet
connection.
To start the disrupter, press the green start button on the panel.
How to clean the system ?
-
Wash with NaOH 0.1M, and then with 500 ml of H2O. Use H2O with squeeze
bottle. Do not use pasteur pipet ! ! !
Use a pressure of 500 bar.
Then start the cycle.
Advise : If you haven’t use the disrupter for a long time, clean the vessel with EtOH
20-30%.
Contact :
Dominique Gozlan
04 66 82 82 60
06 07 65 08 45