SUPPORTING INFORMATION For The Magnesium Transporter 5 Plays an Important Role in Mg Transport for Male Gametophyte Development in Arabidopsis Xiao-Feng Xu, Bo Wang, Yue Lou, Wen-Jian Han, Jie-Yang Lu, Dan-Dan Li, Le-Gong Li, Jian Zhu*, Zhong-NanYang* *To whom correspondence should be addressed. E-mail: znyang@shnu.edu.cn (ZN.Y); zhujian1@tongji.edu.cn (Z.J). This file including Table S2. Transmission efficiency of the heterozygous mutant of mgt5 and identification of MGT5+/- progeny via self-pollination. Table S2. Transmission efficiency of the heterozygous mutant of mgt5 and identification of MGT5+/- progeny via self-pollination. Parental Genotypes Male WT MGT5+/MGT5+/- Female MGT5+/WT MGT5+/- Genotypes of Progeny MGT5+/+ 106 212 112 MGT5+/108 0 110 mgt5-/0 0 2 Total TEf TEm 214 212 224 50.50% NA NA NA 0 NA The transmission efficiencies (TE) were calculated according to the following equation: TE = number of progenies with T-DNA insertion/number of total progeny × 100%. For normal transmission, 50% reciprocal crosses were expected. mgt5+/- was supplemented with 100 µM magnesium sulfate. TEF, female transmission efficiency; TEM, male transmission efficiency; NA, not applicable.