Study Guide Biotech 2

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1. When creating a tissue culture lab
what is something that you do not
have to consider?
15.
16.
17.
18.
19.
2. What is the point of plant tissue
culture?
3. Of the following lab procedures
which one best describes an aseptic
practice?
4. In your lab, what ingredient should
keep a PH of 7 until adjusted?
5. In an aqueous saline solution, which
term best describes the salt(saline)
ingredient?
6. What is neutral on the PH scale?
7. What is a use for a reagent:
8. What color pipette is used for 25mL
measurements?
9. Which tool is used to perform
separation of cells?
10. How do you use of the
micropipette?
11. Bioremediation is:
12. A hybrid is:
13. What Genetic Engineering term
best describes using
molecular scissors to remove a
segment of DNA?
14. Cells “turning off” or “turning on” a
gene is a process known as:
The blueprint of all of the genetic
possibilities of a plant or animal is
called:
Another name for rDNA is:
Cloning is:
Progeny testing is used for:
An example of selective breeding is
collecting semen from a bull and using
it on your cows.
20. When managing livestock, do they have
surrogate mothers?
21. What is a plasmid in relation to
genetic engineering, is it a vector?
22. What is BT Corn
23. What are Buffers, reagents and
solutions
24. What are Careers you learned in
Biotechnology?
25. What is Biotechnology
26. What is an example of a genetic
mutation
27. What are 2 risks we discussed in
biotechnology?
28. Provide 2 reasons why Ethics are
such a large part of Biotechnology?
29. What are biofuels? What is an
example of them?
30. What are two examples of
genetically modified organisms?
31. What are two reasons some
solutions PH not change?
32. What is a PIP
33. What is PPA
34. What are things you should
consider when you are
transplanting a plant?
35. Explain the process of putting a
plant into a pot?
36. Compare and contrast pollination,
and fertilization.
37. Define Plant tissue culture:
38. When using a thermometer what
are two things you need to
consider?
39. What are growth regulators? Why
do you need them in
tissue culture?
40. If I have Bleach that needs to be
diluted from 12%to 3%, and I need
a total of 200mL of it how much
Solute do I need, and how much
Solvent do I need?
41. If you need to adjust the PH of
Water to 5.5, what do I need to add
to my water to lower the PH?
42. If I need to adjust the PH of water
to 8.5 what can I add to make my
PH higher?
43. When you need a total of 200 ml
bleach solution to be diluted down
to 2% bleach solution from a 8.5%
bleach solution how much solute
and how much solvent should you
have?
44. When you are given 40ml of a 9%
bleach solution and you need to
dilute it down to a 4% bleach
solution how much water should
you add?
45. Why is the environment for tissue
culture so important?
46. What size should your cauliflower
be cut at in the beginning of plant
prep?
47. What do Buffers, and PH have in
common (complete sentences)
48. What do you add to your media to
decrease the PH
49. How long do you stir the plant in
soapy water?
50. What are two safety procedures
you should
consider while doing media prep?
51. Define Aseptic Technique
52. Name a solution, and identify the
solute and the solvent.
53. When a substance needs to be
more basic, what number range
does it need?
54. What can you add to a substance to
make it more acidic?
55. If your PH tester is testing H20 at
7.9, and you need a true PH of 5.7;
what should your PH tester read
when you alter your PH?
56. What type of stem is considered an
above ground horizontal stem?
57. Which motion is the least important
when doing parliamentary
procedure?
58. What is the term used to describe a
plant that has its cambium layer
removed and wrapped with
sphagnum moss and saran wrap to
grow roots on it?
59. What is the term used to describe
what type of stem a corm i
60. Compare and contrast asexual
propagation to sexual propagation?
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