Biological active substances in plant raw material of Calligonum
aphyllum (PALL.) GURKE.
Д. Халелова, И. Ищанова, А. Куанышева, К. Жеткиншекова
L.N. Gymilyov Eurasian National University,
010008, Kazakhstan,Astana, Mirzoyana street 2
E-mail: rakhmadiyeva_sb@enu.kz
Plants of Calligonum L. genus have been used in traditional medicine like a vulnerary,
hypoglycemic agent, in case of peritoneal cavity diseases, toothache from olden days [1]. The
object of the investigation is Calligonum aphyllum (PALL.) GURKE which growing in Aktobe region.
During the experiment, the optimum conditions of extraction of biological active substances
of Calligonum aphyllum were find by the following characteristics: best extragent, the degree of
fineness, extragent -plant raw material ratio, time of extraction, drafting ratio. The best extragent is
30 % ethanol, the degree of fineness is 5 mm, extragent -plant raw material ratio- 1:1, time of
extraction 3 days, drafting ratio- 3 times,
By the method of thin-layer chromatography with using specific locating agents and
comparing with significant samples (SSS) were found that all organs of Calligonum aphyllum
contain of flavonoids, amino acids (leucine, serine), carbohydrates (glucose, lactose), saponins,
indole alkaloids. By the method of electrophoresis in above ground part of the plant citric acid was
find.
By the method of spectrophotometric detection with using correction factor on state standard
sample of tannin the amount of tanning substances were find in dry raw material, in roots 12.8%, in
above ground part of the plant- 4.01%; flavonoids on state standard sample of quercetine-in above
ground part of plant- 1.97%, in roots- 4.14 %; carbohydrates on state standard sample of glucose- in
above ground part of plant- 1.9%, in roots- 1.7 %; saponins on state standard sample of white
saponin in above ground part of plant- 2.5%.
The amount of organic acids was determine in leaves (1.07%), blooms ( 0.95%) by the
neutralization method with methyl orange indicator. [2] .
Литература
[1] El-Hawary ZM and Kholief TS (1990): Biochemical studies on some hypoglycemic agents (II) effect of
Calligonum comosum extract. Arch. Pharm. Res. (13): 113-116.
[2] Государственная фармакопея РК 1изд. –Т. 1.- С. 161-163.