OHS026
Safe Work Procedure
Faculty/Division
Medicine
Document number
SOMS GBD SWP 033
School/ Divisional Unit
Medical Sciences
Initial Issue date
20-04-2010
Current version
1.0
Current Version
Issue date
20-04-2010
Next review date
20-04-2013
The Writing Safe Work Procedures Guideline (OHS027) should be consulted to assist in the completion of this
form.
Safe Work Procedure Title and basic description
Title: Safe
Work Procedure for operating SNAPid protein detection system
Description: This safe work procedure details:
1.
Use of pump
2.
Use of SNAPid protein detection system
Associated risk assessment title and location: RA Use RNAPid protein detection system 022, RA waste management 015 located in
room 209B.
Describe the activity or process
1.
2.
3.
4.
5.
6.
Open the blot holder lid, and wet the white surface of the blot holder with distilled water,
Place the blot membrane in the blot holder with the protein side down, and roll the blot membrane gently.
Place the spacer on the top of blot membrane and roll again.
Close the blot holder lid firmly.
Open the lid of system and place the blot holder in chamber. Close and latch lid.
Add blocking solution (0.5% Skim milk or BSA) in wells of blot holder. Turn on the pump and apply vacuum to wells using
knobs on the system.
7. Add primary antibody in wells of blot holder, and apply vacuum after 10-minute incubation at room temperature.
8. Wash 3 times with wash buffer (0.1% PBS-Tween 20 or TBS-Tween 20) with vacuum on. Turn off vacuum.
9. Add secondary antibody in wells of blot holder, and apply vacuum after 10-minute incubation at room temperature.
10. Wash 3 times with wash buffer with vacuum on. Turn off vacuum.
11. Take out the blot membrane. Make sure the pump is switched off.
List all resources required including plant,
chemicals, personal protective clothing and
equipment, etc
SNAP id protein detection system, Pump, Blocking solution (0.5% Skim milk or BSA) , Wash buffer (0.1% PBS-Tween 20 or TBSTween 20), Primary and secondary antibodies, Latex gloves, Laboratory gown, Enclosed shoes.
List potential hazards and risk controls including
specific precautions required

Potential electrical hazard – annual inspection of electrical equipment and ensure inspection tag is current.
___________________________________________________________________________________________________________
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Page 1 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007
List emergency shutdown instructions

Pump and the SNAPid system have “OFF” switch that can be used in emergency.

Shut all electrical equipment off at power point in case of emergency.
List clean up and waste disposal requirements
Dispose of vacuumed solution through Chemical Waste
List legislation, standards and codes of practice used
in the development of the SWP
NSW OHS Act 2000
NSW OHS Regulation 2001
Australia Dangerous Goods Code
Code of Practice for the Labeling of Workplace Substances
AS/NZS 2243.2:2006. Safety in laboratories. Part 2: Chemical aspects
Australian Standard AS2243.6-1990. Safety in laboratories. Part 6: Mechanical Aspects.
Australian Standard AS2243.7-1991. Safety in laboratories. Part 7: Electrical Aspects.
AS/NZS 2161.1:2000 Occupational Protective Gloves – Selection, Use and Maintenance
Safe Work Procedure Form (OHS026)
UNSW Hazardous Waste Disposal Procedure
Supervisory approval, training, and review
Supervisor: Prof E Burcher
Signature:
Plant custodian:
Signature
List competency required – qualifications, certificates, licensing, training - e.g. course or instruction:
SWP review date: 20-04-2013
Responsibility for SWP review: Fei Shang
___________________________________________________________________________________________________________
___________
Page 2 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007