1.0
PURPOSE
1.1
2.0
SCOPE
2.1
3.0
To lay down the procedure for sampling of water for chemical and
microbiological Analysis
This SOP covers the sampling procedure for potable water and purified water
RESPONSIBILITY
3.1
4.0
ACCOUNTABILITY
4.1
5.0
Microbiologist / QC Chemist
Deputy Manager-QC
PROCEDURE
5.1
CHEMICAL ANALYSIS
 Microbiologist / QC Chemist shall collect samples
 Samples shall be collected as per the sampling plan and frequency as per
Schedule.
5.1.1
5.1.2
5.1.3
5.1.4
5.1.5
5.1.6
Use Clean 500 ml and 250 ml glass bottles having stopper for chemical
analysis for Potable and Purified water respectively.
Label the bottle in Format No.: xxxxx and Rinse with purified water.
Collect about 500 ml of Potable water in the pre rinsed bottles and 250 ml
of purified water from respective sampling points.
Stopper the bottle tightly and bring it to the lab for analysis.
Enter the details like sampled by, Quantity, Date of sampling, sampling
point name and number as per xxxxx and carry out analysis as per current
version of specification.
After completion of analysis (If it passes) inform to production block
incharge to write the A.R.Number daily report.
5.2
PREPARATION OF 70% IPA
5.2.1
5.3
Prepare 70% IPA as per current revision as per SOP No xxxxx
MICROBIOLOGY ANALYSIS
5.3.1
Clean 250ml autoclavable, screw-capped, glass bottles and rinse with
purified water.
5.3.2
Add 0.1 ml of a 5% Sodium Thiosulphate and 0.1 ml of 15% EDTA
solution in the bottles (For potable water sampling).
5.3.3
Close the bottle with cap and wrap the neck securely with aluminum foil
using rubber bands. Sterilize then at 15-psi pressure, temp 121°C for 20
minutes.
Follow the procedure for collection of water sample for microbiology as
given below.
5.3.4
5.3.5
5.3.6
5.3.7
5.3.8
Collect the water from each sampling point as per schedule.
Label the bottle stating the Name and number of the collection point and
date of sampling as per xxxxx
Before collection, wipe the tap/valve with cotton soaked in 70% Isopropyl
Alcohol.
Open the tap/valve and allow the water to run for three minutes.
5.3.9
Hold the sterile bottle near the sampling point and unwrap the aluminum
foil and open the bottle, take care not to touch the inner surface of cap or
the neck of the bottle to any other non-sterile part.
5.3.10 Collect the water in the bottle, and immediately close it with the cap. Do
not fill the bottle up to the neck.
5.3.11 Close the tap/valve.
5.3.12 Wrap the neck of the bottle with aluminum foil, securing with rubber
bands to avoid contamination during transportation.
5.3.13 Bring the sample to laboratory and enter the details in register
5.3.14 Carry out the analysis with in 2 hours after collection of samples or store
in the refrigerator at 2-8 °C.
5.3.15 Before analysis allow the Refrigerated samples to room temperature and
perform the test with in 24 hours.
 Keep sampling bottles closed until it is to be sampled.
 The Microbiologist shall do the sampling.
 Care should be taken while collecting the samples that the collection
process will be representative of water being tested.
 Keep air space in the bottle (at least 2.5cms) to facilitate mixing by shaking
before examination
END OF DOCUMENT