Unit 5: Plants Lesson 2: Leaves LEAVES: What are the functions of leaves? Site of photosynthesis o Leaf cells absorb energy from sunlight through organelles called chloroplasts. When solar energy is absorbed, the plant cell uses it to convert carbon dioxide and water into glucose and oxygen Gas exchange o Gas exchange between the interior of the plant and its environment is necessary. The surface (epidermis) of most plant leaves contain may pores through which gases can pass in and out. In photosynthesis, the plant uses carbon dioxide gas and releases oxygen gas and water vapours through these pores. In cellular respiration, the plant uses oxygen and produces carbon dioxide. Storage o The sugars (glucose) that are produced during photosynthesis are stored in the leaves as well as other parts of the plants Protection from predators o Ex: cacti leaves are reduced to sharp spines; some leaves have surface hairs or toxins that prevent herbivores from eating it What is the structure of leaves? The structure of a leaf helps it to carry out its functions. External Structure: The veins of the leaves contain vascular tissue. Recall that vascular tissue allows for the transport of water and nutrients and also support the plant body. Internal Structure: The epidermal cells are tightly packed in a single layer and covered by a waxy coating called a cuticle. The cuticle prevents water loss and provides a physical barrier against bacteria, moulds and insects. Epidermal cells do not contain chloroplasts, so they do not perform photosynthesis. Epidermal cells are transparent, so light can pass through them to the cell within the leaf. Chloroplasts are found mainly in the cells of the mesophyll, which means ‘middle leaf’. This ground tissue is specialized for photosynthesis. There are two parts to the mesophyll: 1. palisade mesophyll (region directly under the upper epidermis). The tightly packed, elongated arrangement maximizes the amount of light the plant can collect for photosynthesis 2. spongy mesophyll (region directly beneath the palisade). The loosely packed layer with lots of air spaces allow for gas exchange between the mesophyll cells and the atmosphere through stomata. Stomata & Guard Cells A stoma (plural: stomata) is an opening in the epidermis of a leaf, through which gases pass in and out. Two kidney-shaped cells, called guard cells, control the opening and closing of a stoma. In terrestrial plants, most of the stomata are in the lower epidermal layer, below the spongy mesophyll. When stomata are open, carbon dioxide can enter the leaf and oxygen can escape. This helps the plant photosynthesize. Ideally, plants would open their stomata whenever it was sunny. However, when stomata are open, water vapour can also escape. A very thin leaf could dry out and die very quickly on a sunny or windy day. Preventing too much water loss is therefore a major concern for many plants. Guard cells are an adaptation to help the plant conserve water by altering their shape in response to water levels in the leaf. If there is a good supply of water within the leaf, the guard cells expand and bend apart -- opening the stomata. If there is a shortage of water, the guard cells become soft and collapse -- closing the stomata. Guard cells also have a mechanism that responds to light levels. This lets them close the stomata at night when carbon dioxide is not needed because there is not light for photosynthesis. In leaves, the vascular tissue (xylem and phloem) are arranged into veins that run through the spongy mesophyll. Xylem carries water, mineral and nutrients upwards from the roots to the top of the plant. Phloem carries food(sugars) from one part of a plant to another. It can move upwards or downwards. Photosynthesis and Cellular Respiration Photosynthesis Light is used with Carbon dioxide from the air and water from the root system to produce glucose and oxygen. This occurs during the day. 6CO2 + 6H2O + Light Energy = C6H12O6 + 6O2 Cellular Respiration The glucose is broken down (with oxygen from the air) to create ATP with carbon dioxide and water as by products. C6H12O6+6O2 ----------> 6CO2+6H2O+36ATP Questions: 1. What functions do leaves serve to the plant? 2. How do monocot and dicot leaves differ? 3. Describe the function of the following: epidermis, cuticle, mesophyll, guard cells. 4. Explain how the organization of cells in the palisade mesophyll and spongy mesophyll in a typical vascular plant help fulfill the leaf’s role? 5. Explain how the guard cells work to allow the stomata to open and close. Why does stomata open and close? The leaf is the primary photosynthetic organ of the plant. It consists of a flattened portion, called the blade, that is attached to the plant by a structure called the petiole. Sometimes leaves are divided into two or more sections called leaflets. Leaves with a single undivided blade are called simple, those with two or more leaflets are called compound. The outer surface of the leaf has a thin waxy covering called the cuticle (A), this layer's primary function is to prevent water loss within the leaf. (Plants that leave entirely within water do not have a cuticle). Directly underneath the cuticle is a layer of cells called the epidermis (B). The vascular tissue, xylem and phloem are found within the veins of the leaf. Veins are actually extensions that run from to tips of the roots all the way up to the edges of the leaves. The outer layer of the vein is made of cells called bundle sheath cells (E), and they create a circle around the xylem and the phloem. On the picture, xylem is the upper layer of cells (G) and is shaded a little lighter than the lower layer of cells phloem (H). Recall that xylem transports water and phloem transports sugar (food). Within the leaf, there is a layer of cells called the mesophyll. The word mesophyll is greek and means "middle" (meso) "leaf" (phyllon). Mesophyll can then be divided into two layers, the palisade layer (D) and the spongy layer (F). Palisade cells are more column-like, and lie just under the epidermis, the spongy cells are more loosely packed and lie between the palisade layer and the lower epidermis. The air spaces between the spongy cells allow for gas exchange. Mesophyll cells (both palisade and spongy) are packed with chloroplasts, and this is where photosynthesis actually occurs. Epidermis also lines the lower area of the leaf (as does the cuticle). The leaf also has tiny holes within the epidermis called stomata. Specialized cells, called guard cells (C) surround the stomata and are shaped like two cupped hands. Changes within water pressure cause the stoma (singular of stomata) to open or close. If the guard cells are full of water, they swell up and bend away from each other which opens the stoma. During dry times, the guard cells close. Color the structures underlined above. Make sure that the entire picture is colored and that the color matches the words. For simplicity only part of the picture is labeled. Epidermis (yellow) Guard cells (pink) Spongy Mesophyll (light green) Palisade Mesophyll (dark green) Bundle Sheath(dark blue) Phloem (purple) Xylem (orange)