Department of histology and embryology LF MU Brno 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 Labium oris (HE) Apex linguae (HE) Papilla circumvallata (HE) Tonsilla lingualis (HE) Palatum molle (HE Tonsilla palatina (HE) Tooth (HE) Glandula parotis (HE) Glandula submandibularis (HE) Glandula sublingualis (HE) Oesophagus (HE) Cardia (HE) Fundus ventriculi (HE) Pylorus (HE) Duodenum (HE) Intestinum tenue (HE) Intestinum crassum (HE) Appendix (HE) Anus (HE) Hepar (HE) Hepar (Azan) Vesica fellea (HE) Pancreas (HE) Concha nasi (HE) Epiglottis (HE) Larynx (HE) Trachea (HE) Elastic cartilage (orcein) Pulmo (HE) Ren (HE) Ren (Weigert-van Gieson) Calyx renalis (HE) Ureter (HE) Vesica urinalis (HE) Urethra feminina (HE) Testis (HE) Epididymis (HE) Funiculus spermaticus (HE) Vesicula seminalis (HE) Prostata (HE, Azan) Penis (HE, HES) Ovarium (human, monkey) (HE) Ovarium (cat) (HE) Corpus luteum (HE) Tuba uterina – ampulla (HE) Tuba uterina – isthmus (HE) Uterus – proliferative phase (HE) Uterus – secretory phase (HE) Vagina – glykogen (Best´s carmine) Vagina (HE) 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 SLIDES IN BOX Labium minus (HE) Hypophysis cerebri (HE) Epiphysis (HE) Glandula thyreoidea (HE) Glandula parathyreoidea (HE) Corpus suprarenale (HE) Thymus (young) (HE) Thymus (involution) (HE) Muscle artery and vein (HE) Muscle artery and vein (orcein Aorta (HE) Aorta (orcein) Vena cava (HE) Myocardium (HE) Myocardium (Heidenhein) Lymphonodus (HE) Lien (HE) Lien (impregnation) Skin from the top of finger (HE) Skin from the axilla (HE) Skin with hair (HE) Nail (HE) Mamma non lactans (HE) Mamma lactans (HE) Cortex cerebri (HE) Cortex cerebri (impregnation) Cerebellum (impregnation) Cerebellum (Nissl) Medulla spinalis (HE) Plexus choroideus (HE) Ganglion spinale (HE) Ganglion spinale (impregnation) Autonomic ganglion (HE) Peripheral nerve – cross section (HE) Peripheral nerve – cross section (myelin) Peripheral nerve – longit. section (HE) Peripheral nerve – longit. section (myelin) Anterior segment of the eye (HE) Posterior segment of the eye (HE) Fasciculus opticus (HE) Palpebra (HE) Glandula lacrimalis (HE) Cochlea (HE) Auricle (HE, HES) Bone (Schmorl) Ossification (HE) --------------------Umbilical cord (HE, Azan) Placenta (HE) Department of histology and embryology LF MU Brno ATLAS EM – LIST OF PAGES Human ovarian follicle Nucleus of the liver cell Nucleus of the nerve cell Cell nucleus (freeze-fraction) Nucleolus of the embryonic cell Nucleolus of the glandular cell Ring-shaped nucleolus Mitochondria in the liver cell Golgi apparatus in the glandular cell Golgi apparatus in the liver cell Golgi apparatus (detection of acid phosphatase) Lysosomes (detection of acid phosphatase) 1 2 3 4 5 6 7 8 9 10 11 Surface of the oviduct epithelium (REM) Human spermatozoon with flagellum The cell during mitosis Blood capillary – pinocytosis Neutrophilic granulocyte – phagocytosis Cell death – apoptosis Cell death - necrosis Erythrocytes (REM ) Eozinophilic granulocyte Monocyte Lymfocyte 30 31 32 33 34 35 36 37 38 39 40 12 41 Autophagic vacuole in the liver cell Endoplasmic reticulum in the liver cell Endoplasmic reticulum (detection of glucose-6-phosphatase) Glandular cell of pancreas Peroxisomes in the liver cell Peroxisome with nucleoid Microperoxisomes ana peroxisomes (detection of catalase) Centriole in the liver cell Centriole in the fibroblast Glycogen in the cardiomyocyte Lipid droplets in the steroidogenic cell Desmosomes in the epidermal cell Nexus in the ovarian follicle Terminal bar in the oviduct epithelium Microvilli (detection of alcaline phosphatase) Brush border – surface of the enterocytes Kinocilia - the oviduct epithelium 13 14 15 Thrombocyte and erythrocytes in blood capillary Fibroblast Plasma cell Heparinocyte 16 17 18 19 Chondroblast Osteoblast Osteocyte Surface epithelium 45 46 47 48 20 21 22 23 24 25 26 27 Basal labyrinth Glandular cell of pancreas Respiratory epithelium Rhabdomyocyte Cardiomyocyte Leiomyocyte Neuron of spinal ganglion Nerve fibers with sheaths 49 50 51 52 53 54 55 56 28 29 Synaptic nerve endings Myelinized nerve fiber 57 58 Department of histology and embryology LF MU Brno 1 2 3 4 5 6 7 8 9 10 11 12 13 42 43 44 ATLAS EM - DESCRIPTIONS Oocyte (1), zona pellucida (2), follicular cells (3), fibrocyte (4), blood vesel (5) Cell nucleus: euchromatin (1), heterochromatin (2), nucleolus (→) Nucleus of nerve cell with euchromatin: nuclear envelope with numerous pores (→) Inner (1) and outer (2) membrane of nuclear envelope; nuclear pore (→) (freeze fracture) Aktive nucleolus: fibrilar center (*), pars fibrosa (→), pars granulosa () Nucleolus with predominant pars granulosa (); pars fibrosa (→) Ring-shaped nucleolus Mitochondrion: cristae (), matrix (*), mitochondrial granule (→) GA: cisterna (→), immature secretory granules (*), granular endoplasmic reticulum with transport vesicles () GA: dictiosome (1), lysosomes (2) Dictiosomes located near the nukleus(J); detection of acid phosphatase (→) Secondary lysosomes; detection of acid phosphatase (→) in some of them. Autophagic vakuole (), α-granules of glykogen (→) 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 Cisternae of rough endoplasmic reticulum (1), tubules and vesicles of smooth endoplasmic reticulum (*) Granular endoplasmic reticulum; detection of glukoso-6-phosphatase Glandular cell: rough endoplasmic reticulum (1), Golgi apparatus (2), secretory granules (3) Peroxisomes: matrix (1), nucleoid (2); α-granules of glykogen (→) Peroxisome (→) with nucleoid of cristalline structure Microperoxisomes (1) and peroxisomes (2) - detection of catalse Gross section through the centriole Longitudinal section through the centriole; satelite structure (→), microtubule (*) β-granules of glykogen (→) in cardiomyocyte Lipid droplets (1) in steroidogenic cell, mitochondria with tubular cristae (2), vesicles of smooth endoplasmic reticulum (*) Desmosomes (1), bundles of tonofilaments (2) Nexus (→←) Free surface of ciliated epithelium; junctional complex: zonula occludens (→), zonula adherens (), macula adherens (*) Free surface of epithelium with irregular microvilli: detection of alkaline phosphatase (→) Striated border on the surface of enterocytes; zonula occludens (1), zonula adherens (2) The cell with the cilia: cross section through the cilium (1), basal body (2), striated rootlet (3) The surface of the oviduct epithelium in the scanning electron microscope: cells with cilia (→) and microvilli (*) Human spermatozoon: head with acrosome (→), flagellum with axoneme, smooth chorda and mitochondrial sheath () The cell in mitosis: chromosomes (1) and mitotic spindle (2) Blood capillary: nucleus of the endothelial cell (J), pinocytic vesicles (→), erythrocyte (*) Neutrophilic granulocyte with granules (1) and phagosomes (2); segment of nucleus (3) Apoptosis (a way of cell death): condensed chromatin in the nucleus (→), degenerating organelles in cytoplasm (*) Necrosis (a way of cell death): disintergrated nucleus (J), rests of organelles in destroyed cytoplasm (→) Erythrocytes in the scanning electron microscope Eosinophilic granulocyte with two-segmented nucleus and speciphic granules (→) Activated monocyte with numerous phagosomes (F) - macrophage Medium-sized lymphocyte Trombocyte with granules (G) in the fenestrated capillary. Lamina basalis (*) Fibroblast; rough endoplasmic reticulum (1), Golgi apparatus (2), collagen fibrils (3) Plasma cell with rough endoplasmic reticulum (ER) Mast cell (heparinocyte) with dense granules (1); collagen fibrils Chondroblast; rough endoplasmic reticulum (1), Golgi apparatus (2), intercellular matrix with collagen fibrils (3) Osteoblast with rough endoplasmic reticulum. Osteoid (*) Osteocyte during bone resorption. Lacune (1), bone matrix (2) Simple low-columnar epithelium. Free surface (1), basal (2) and lateral (3) cell membrane Basal labyrinth. Basal lamina (1), invaginations of cell membrane (2), mitochondrion (3) The cell of glandular epithelium; rough endoplasmic reticulum (1), Golgi apparatus (2), secretory granules (3) Respiratory epithelium: membranous (→) and granular (*) pneumocytes Cross-striated muscle fiber – rhabdomyocyte: nucleus (1), myofibrils (2) Cardiomyocyte: cell nucleus (1), myofibrils (2), sarcomere () Smooth muscle cells – leiomyocyte: longitudinal (1), cross (2) and oblique (3) section Cytoplasm of pseudounipolar neuron (1), satellite (glial) cell (2), fibrocyte (3), axon (4) with myelin sheath (*) Axons (1) surrounded by Schwann cell (2) Axons (1) with myelin (3) and Schwann sheath (4) Axon (1) with presynaptic ending (2); synaptic vesicles (→) Nerve fiber: axon (1), myelin sheath (2), nucleus of Schwann cell (3); mesaxon (→)