True or False – Sterilization is the process of eliminating all contaminants from the lab bench surface? True
True or False- Aseptic technique is the process of eliminating all contaminants from the lab bench surface? False
True or False – The incubator needs to be set at 55 degrees Celsius to grow a strain of E. coli? False
True or False- African violet plants can be propagated with PTC media at a pH of 5.3. True
True or False- Monocots have two cotyledons when germinating. False
True or False- A Western Blot is performed by running a high voltage through a cassette to transfer proteins to a
membrane? False
True or False- The two most common types of gels are agarose and polyacrylamide. True
True or False- The process of sequencing DNA is called tri-deoxy chain termination sequencing. False
True or False- The Disco Container System is the preferred system when growing WPFs. False
True or False- The purpose of the sterile cotton ball in the bioreactor lab is to filter the air before it enters the media and
to keep out all unwanted contaminants. True.
PTC Stages – Which stage is responsible for hardening the plant from an in vitro environment to soil? Stage 4°
PTC Stages- Stage 3° is responsible for doing what. Shooting and rooting of explants
PTC Stages- This stage is known as subculturing of explants for multiplication. Stage 2°
PTC Stages- Inoculation of explants on sterile media. Stage 1°
PTC Stages- Stage 0° is responsible for? Selection and pretreatment of suitable plants for PTC.
What piece of equipment is used to heat and cool PCR tubes in order to copy DNA? Thermal Cycler
What is the temperature range for the denaturing step in PCR? 94°-98°C
Define PCR. The process of amplifying a single copy or a few copies of DNA
Describe the extension step in PCR. Taq polymerase binds to the primer and “extends” the DNA.
Can Taq polymerase be destroyed by high Temperatures? No, it was found in hot springs.
What technique can you use to breed 1000 or more rare orchids? Tissue Culturing
Define Selective Breeding: The process which humans breed animals and plants to select for certain traits.
Plant growth is controlled by the addition or omission of what? Hormones
What part of the plant can be used as cuttings in plant propagation? Leaf parts/buds/nodes/root segments/stems
Give an example of a plant ovary. Any fruit that has a seed in the middle with a soft tissue surrounding it such as an
apple/peach/pears/ etc
What part of a new plant ruptures out of the seed coat during germination? Radicle
What is the scientific name of fast plants? Brassica rapa
Name a tool that can be used to pollinate a WFP from flower to flower. Beestick or cotton swab
What does PPE stand for? Personal Protective Equipment
Does the laminar flow hood protect the user? No
True or False- Biotechnology is defined as the study of living things. False
How many days from first generation to planting the next generation of fast plants? 40-45 days
What reagent is used in the catalase assay? Hydrogen peroxide
What two reagents are used in the protease assay? Gelatin plug and Meat Tenderizer
Meat Tenderizer was used as a control variable for which lab this year. Bioreactors
Give an example of a biopharmaceutical. Anything plant, animal, or microorganism. Cell lines and synthetic chemicals
such as insulin.
The PV92 lab looked for the presence of this gene which is specific mutation to all primates including humans. Alu
sequence.
What nutrients are found in WFP fertilizer (soil)? Nitrogen, phosphorus, and potassium (N-P-K)
What three soil components make up the global bucket mixture? Peat/perlite/vermiculite
How many people in developing countries live on less than one dollar a day- according to global bucket research? Over
1,000,000
MATH PROBLEM: 100mL of 5% CaCl2
5g of CaCl2 Bring to volume 100mL of H2O
MATH PROBLEM: 250mL of 95% NaCL
237.5g of Sodium Bring to Volume 250ml of H2O
MATH PROBLEM: 500mL of .5% CuSO4
2.5g of CuSO4 Bring to volume 500mL of H2O
MATH PROBLEM: 150mL of LB Broth at a concentration of 20mg/mL Report in grams. 3.0g of Agar Powder. Bring to
volume 150ml of H2O
MATH PROBLEM: 150mL of LB Broth at a concentration of 20mg/mL Report in grams. 3.0g of Agar Powder. Bring to
volume 150ml of H2O
MATH PROBLEM: 200mL of TAE buffer at 20X concentration from a 50X stock. 80mL of 50x TAE and 120mL of H2O
MATH PROBLEM: 500mL of TAE buffer at 1X concentration from a 50X stock. 10mL of 50X TAE and 490mL of H2O
MATH PROBLEM: 500mL of 70% Ethanol from the 95% stock bottle. 368mL of Ethanol and 132mL of H2O
What is the purpose of adding an ice pack when running a Western Blot? To keep the gel cool so it does not warp or
melt.
What ladder was used in the Western Blot Lab? Pre-stained Kaleidoscope Standard.
What type of stain is need to visualize proteins on a PAGE gel? Coomassie Stain
Draw an indirect ELISA Assay (antibody/antigen complex) Antigen/Primary/Secondary/color detection substrate
What does MEGA stand for? Molecular Evolutionary Genetics Analysis
Name a restriction Enzyme used in the digestion of DNA. PstI, HIndIII, BamHI, EcoRI, AluI, HaeIII
MATH PROBLEM: 100mL of 5% CaCl2
5g of CaCl2 Bring to volume 100mL of H2O
MATH PROBLEM: 250mL of 95% NaCL
237.5g of Sodium Bring to Volume 250ml of H2O
MATH PROBLEM: 500mL of .5% CuSO4
2.5g of CuSO4 Bring to volume 500mL of H2O
MATH PROBLEM: 150mL of LB Broth at a concentration of 20mg/mL Report in grams. 3.0g of Agar Powder. Bring to
volume 150ml of H2O
MATH PROBLEM: How many grams are needed to make 400mL of lemonade at a concentration of .50g/mL. 200g of
powdered lemonade and bring to volume 400mL of H2O
MATH PROBLEM: 200mL of TAE buffer at 20X concentration from a 50X stock. 80mL of 50x TAE and 120mL of H2O
MATH PROBLEM: 500mL of TAE buffer at 1X concentration from a 50X stock. 10mL of 50X TAE and 490mL of H2O
MATH PROBLEM: 500mL of 70% Ethanol from the 95% stock bottle. 368mL of Ethanol and 132mL of H2O