Central Insecticides Lab
Pesticides are inherently toxic in nature, they are more likely
to affect the health of human beings, particularly in agricultural
sector. In India, there is a comprehensive legislation known as
Insecticides Act, 1968 which regulates the import, manufacture,
sale, transport, distribution and use of insecticides with a view to
prevent risk to human beings or animals and the matters
connected therewith. By virtue of the Section 16 of the said Act,
the Central Government has established a Central Insecticides
Laboratory.
Functions of CIL: The functions of the Laboratory as envisaged under
Rule 5 of Insecticides Rules 1971 are as follows: To analyze such samples of insecticides sent to it under the Act by
any officer or authority and submission of
to the concerned authority.
certificates of analysis
 To carry out such investigations as may be necessary for the
purpose of ensuring the conditions of Registration of Insecticides.
 To determine the efficacy and toxicity of insecticides;
To carry out such other functions as may be entrusted to it by the
Central Government or by a State Government with the permission
of the Central Government & after consultation with the Central
Insecticides Board.
Activities of CIL
1. To verify Quality of samples of Insecticides, submitted by any
Officer or Authority Authorized by the Central or State Government.
2. To investigate Insecticides with a view to verify conditions of
registration.
3. To determine efficacy and Toxicity of Insecticides; and
4. Any other function as may be entrusted by the Central
Government or by a State Government with the permission of the
Government and after consultation with the Central Insecticides
Board.
Division wise Details of Activities & Achievements:

CHEMISTRY DIVISION

BIOASSAY DIVISION

MEDICAL TOXICOLOGY DIVISION

PACKAGING AND PROCESSING DIVISION
CHEMISTRY DIVISION:
OBJECTIVE:
To discharge the techno-legal duties as specified under Rule 5 of
Insecticides Rules, 1971.
ACTIVITIES:
a. To analyze samples of pesticides sent to it under the Act by any
officer or authority authorized by the Central or State Governments and
submission of certificate of analysis to the concerned authority;
b. To carry out such investigations as may be necessary for the purpose
of ensuring the conditions of Registration of insecticides;
c. To validate methods of analysis for newly introduced pesticides for
their adoptability.
d. To facilitate formulation of Indian Standards on pesticides.
e. To carry out investigational analysis on imported commodities,
suspected to contain pesticides.
Samples analysed during 2007-08 to 2012-13
S.No.
Year
Target
Sample Received
Under Act and
Reported
No. of replicate
samples analysed
for verification
Total
No. of
samples
analysed
1.
2007-08
1600
1057
2599
3656
2.
2008-09
1600
929
1787
2716
3.
2009-10
1600
733
1884
2617
4.
2010-11
1600
947
1830
2781
5.
2011-12
1600
954
1882
2836
6
2012-13*
1600
856
1188
1828
*Up to Feb.-2013.
Accreditation of the Chemistry division of CIL: Division had taken initiatives for
accreditation of the division and got accreditation certificate valid up to 03.07.2014.
Analysis of samples for ILCP: : As per the requirement of National Accreditation
Board for Testing and Calibration Laboratories (NABL), Inter-Laboratory Collaboration
Programme is under progress.
Additional activities:
1. Pre Registration Verification of pesticide samples sent by the Secretariat of
CIB & RC:
The programme for testing the technical / formulated pesticides to be registered
under section 9(3), 9(3b) & 9(4) for ensuring quality standards and verification of
their method of analysis has been taken up by this Division. 129 samples have
been analysed for Pre Registration Verification from April, 2012 to March, 2012
and 91 samples analysed during April, 2012 to November 2012.
2.
Analysis of samples for repetition/confirmation:
Chemistry Division is analyzing samples for repetition /confirmation of results
which are incidental and supplemental to the analysis of samples for statutory
quality control. During the year 2011-12 , a total of 2836 No. of samples have been
analysed and 1568 samples during April, 2012 to November,2012.
3.
Maintenance of Pesticide Repository:
Technical/Reference standards of all available pesticides are procured from the approved
source in regular manner. These are supplied to Regional Pesticides Testing Laboratories/
State Pesticides Testing Laboratories as and when asked for.
4. Formulation of BIS Standard and Technical comments on printed and drafted Indian Standards
specification:
Technical comments are furnished on the subject matters to expert committees of the Bureau
of Indian Standards, FAD - I (Pesticides & Pesticide Residue Analysis).
5. Technical Guidance to State Pesticides Testing Laboratories:
The Analysts of the SPTLs and Insecticide Inspectors have been provided Technical support
by the chemists of this division. Training in-situ including other relevant assistance e.g.
copies of the relevant specifications, standard reference material, handling of sophisticated
equipments, list of equipments necessary for pesticide Testing Laboratories and other
infrastructure etc. are provided on demand.
Guidelines
Chemistry Division of CIL is analyzing the samples of pesticides and their
formulations for the quality control. The methodology adopted by the Division is
Bureau of Indian Standards (BIS) methods/Approved methods of pesticides and
their formulations by the Registration Committee.
Bio-Assay Division
Bioassay Division of Central Insecticides Laboratory (CIL) is established under
rule 5(d) of Insecticides Rules, 1971 of the Insecticides Act, 1968 under scheme
“Implementation of Insecticides Act.”
Mandate
The main mandate of this division is to study the bio-efficacy of pesticides
against target pest species including quality control of bio-pesticides
The bio-efficacy of pesticides is evaluated in terms of pest mortality/reduction in
pest population due to the application of pesticides
Objectives
A. To evaluate the pesticides for:-
i. Bio-effectiveness
ii. Phytotoxicity
iii. Compatibility with other pesticides
B. Evaluation of pesticides of plant origin.
C. Development of Bioassay techniques for quality control.
D. Preparation of information/material and training of scientists.
FUNCTIONS


Post registration verification of bio-effectiveness and phytotoxicity of chemical
and biological pesticides against target pests on approved crops/habitat under
the Insecticides Act, 1968
Pre registration verification of bio-pesticide samples received from CIB & RC for
quality control
ACTIVITIES





To maintain laboratory cultures of test pests (insects, fungi, bacteria, viruses,
etc.)
To maintain weed seeds as test material for conducting bio-efficacy tests
To conduct laboratory and field trials/experiments for studying bioeffectiveness and phytotoxicity of pesticides
Preparation and submission of technical reports of bioassay division
To impart trainings to the state/central officials on quality control of biopesticides
Physical Targets and Achievements of Central insecticides laboratory (CIL)
for the last five years (2007-08 to 2011-12)
Division
No. of samples
2007-08
2008-09
2009-10
2010-11
2011-12
Cap.
Ach.
Cap.
Ach.
Cap.
Ach.
Cap.
Ach.
Cap.
Ach.
Chemistry
1600
1142
1600
982
1600
787
1600
1020
1600
1083
Medical
20
20
20
20
20
20
20
20
20
19
60
91
60
89
60
67
60
72
60
75
--
112
-
120
-
161
-
244
-
174
139
150
78
150
94
150
74
150
50
Bioassay
Pesticide
Bio-pesticide
Packaging 150
Cap – Capacity to analyze Ach.-Achievements
NABL ACCREDITATION
Bioassay Division of CIL obtained accreditation of NABL as per ISO// EC
17028:2005 in the field of Bio-logical testing. The quality control of Biopesticides is being tested for the following parameter.
Name of the Biopesticides
Trichoderma viride &
T. harzianum
Parameters tested
CFU (Colony Forming Units) Counts
Antagonistic capability
pH
Suspensibility
Nuclear Polyhedrosis Virus (NPV)
Pathogenic contaminants
POB count
Pathogenic contaminants
pH
Moisture content
Suspensibility
Pseudomonas fluorescens
Viable cell count
Antagonistic capability
Moisture content
Pathogenic contaminants
Suspensibility
pH
Beauveria bassiana
CFU count
pH
Moisture content
Suspensebility
Metarhizium
anisopliae/clamydosporium Verticillium lecanii
Paecilomyces lilacinus
Pathogenic contaminants
-do
-doCFU count
Ph
Moisture content
Suspensebility
.Bacillus thuringiensis
Antagonistic capacity
Pathogenic contaminants
Heat resistant spore count (CFU)
pH
Moisture cont
Suspensibility
MAINTENANCE OF CULTURE
The Division maintains cultures of crop insects, household insects, stored
grain pests, fungi and seeds of weed species for tests and trial purposes .
MEDICAL TOXICOLOGY DIVISION
Any insecticide which is to be imported or manufactured for use in the country
is to be compulsorily registered by the Registration Committee constituted under
Section 5 of the said Act. The registration of a pesticide is to be granted after the
Committee is satisfied as regards the efficacy and safety of the product. The Medical
Toxicology Division is created in order to verify the safety claims made by the
manufacturers through submission of the data on various parameters at the time of
grant of registration. Sometimes, the adverse effects come to limelight in the due
course of its use. To establish its adverse nature, this Division has to generate data
and advice to the Government to take suitable course of action. In such cases, there
is a need for adopting the risk management strategy in order to reduce the hazards to
the human beings and the environment. The Division is a highly specialized discipline
of medicine which inter alia looks after the safety to human beings, animals and the
environment.
Objectives and Activities/Functions
Keeping in view the above background, as per the Insecticides Rules, 1971, Medical
Toxicology Division is to undertake functions like risk identification, risk assessment,
risk management and risk communication. For the purpose of safety evaluation, this
Division has to generate data on different parameters of toxicity as follows:a)
b)
c)
d)
e)
f)
g)
Acute, Oral/Dermal LD 50 study
Standardization for estimation of cholinesterase in rat and human serum
Short term studies
Sub-acute/ sub-chronic oral toxicity study
Chronic toxicity study in mammals.
Health monitoring study
Impart training to Medical doctors on Diagnosis, Management and
Treatment of Pesticide Poisoning
poisoning
in the areas having high incidence of pesticide
TECHNICAL PROGRAMME/PROGRESS
The Medical Toxicology Division evaluated 20 samples for Acute oral toxicity studies as against the Annual
Capacity of samples for every year
The other activities include:
(i)
(ii)
(iii)
(iv)
(v)
(vi)
Toxicity/Safety evaluation/status of pesticides before and after introduction.
Toxico-vigilance activities regarding pesticides.
Epidemiological study/current status on pesticide poisoning and related health effects.
Training on safe and judicious use of pesticides for users/farmers
Training on diagnosis, management and treatment of pesticide poisoning for medical doctors and
para-medicals.
Preparation of technical material on pesticide toxicity.
The Medical Toxicology Division is providing the information regarding - technical programmes
activities, target, achievements and progress in the form of EFC Memo, Annual Report, Annual Plan, Physical &
Financial Review, Mid-Term Appraisal to the Department of Agriculture & Cooperation in the programme
“Strengthening and Modernization of Pest Management Approach in India”.
The M.T. Division is also collecting information on Current status of Pesticide Poisoning Cases for Safety
Evaluation and Status of notification of functionaries for reporting poisoning cases from Zonal Conferences.
ACHIEVEMENTS/HIGHLIGHTS
Acute Oral Toxicity Study (LD50): for the last five years
Year
Capacity
Achievements
2008-09
20
21
2009-10
20
20
2010-11
20
20
2011-12
20
2012-13
20
20
18
(Up to feb 2013)
1. Conducted IAEC Meeting/s
2. Prepared module on safety status and use of pesticides for training to the Medical
doctors and
presented in CIB meeting
3. Prepared material for the finalization of Module on doctors Training Programme
4. Prepared agenda / documents for the Management Review and management
review committee and NABL accreditation
Animal House Modernization
As per the requirement of CPCSEA/NABL/GLP, the process for updation and
modernization of the animal house of Medical Toxicology Division, CIL is in progress.
after Administrative approval form DAC.
NABL Accreditation
This division has initiated the process for submission of application to NABL
for getting NABL accreditation.
Reports and Publications :
I)
Publications
A) Know Your Pesticides.
B) Treatment of Pesticides Poisonings .
C) Avoid Pesticides poisoning- Do,s and Don’ts( Both Hindi and English).
D)
II)
Report on Establishments on harmonized Pesticides Poisoning data base in India.
Posters and pamphlets on
A) Do,s and donts regarding
i) Safe and Judicious Use of pesticides:
ii)
During Storage
iii) During handling
iii) During Use
iv)
After use
B) Definition of Pesticides
C) Classification of pesticides
D) Brief Description of Insecticides Act and Rules
E)
Emergency management of Pesticides Poisoning
F) Diagnosis and Treatment of pesticides Poisoning
G) Documentary on safe use of pesticides
Guidelines: Medical Toxicology Division is conducting Acute Oral Toxicity
studies as per OECD Guidelines. available on website of OECD.
H)
PACKAGING DIVISION
Packaging Division is one of the four Divisions of Central Insecticides Laboratory
(CIL), established u/s 16 of Insecticides Act, 1968. Significant activities of the Division
are as under:-
Objectives:
1.
2.
3.
4.
Pre and post registration verification of the packaging and labeling claims
/ requirements made by the manufacturers / registrants.
Verification / analysis of the packaging and labeling samples received
under Section 5 (C) of the Insecticides Rules, 1971 in the context of
conditions laid down on the certificate of registration issued under
Insecticides Act, 1968.
Technical guidance to the Bureau of Indian Standards, in formulating,
updating and amending the standards of pesticides quality control, safety
storage, transportation and use.
Imparting training to the enforcement functionaries of States/UTs on
various aspects of pesticides relating to packaging, labeling and other
requirements.
ACTIVITIES
a.
b.
c.
d.
Verification / analysis of the packaging, labeling & leaflet samples received
under Section 5 (C) of the Insecticides Rules, 1971, in the context of
conditions laid down on the certificate of registration issued under
Insecticides Act, 1968.
Verification of data on shelf-life claims of pesticides and their formulations
as per protocol approved by the Registration Committee and Bureau of
Indian Standards (BIS) on Quality Control and Packaging.
To render technical guidance to the state Department of Agriculture
functionaries and the Bureau of Indian Standards (BIS) and other
scientific bodies dealing with matters relating to the packaging, labeling,
quality control, safety and other aspects of pesticides.
Development of eco-friendly and economic pesticidal formulations and
packaging system/devices at laboratory scale.
TARGET-ACHIEVEMENTS:
The annual capacity of the Division is to analyze 150 samples.
1. Pesticide sample for testing of packaging, labelling & leaflet parameters are
required to be drawn by State Department of Agriculture functionaries on
regular basis as per the target earmarked to them.
2. A copy of each Label, Leaflet and the certificate of Registration is required to
be sent with the samples of pesticides drawn under Rule 5(C) of Insecticides
Rules 1971 while forwarding the sample to CIL.
Target and Achievement of the Packaging Division (CIL) for last five years (200708 to 2011-12) including current year (2012-13 upto February, 2013) is as
under:
Year
Annual Target
Achievement
2007-08
150
139
2008-09
150
78
2009-10
150
94
2010-11
150
74
2011-12
150
60
2012-13
150
64(
2013)
upto Feb.,2013)
Total
509