SUPPLEMENTARY MATERIAL
Two new ionone glycosides from the Roots of Rehmannia glutinosa
Libosch.
Wei-sheng Fenga*, Meng Lib, Xiao-ke Zhenga, Na Zhanga, Kai Songa, Jian-chao
Wanga, Hai-Xue Kuangb
a
School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou
450046, PR China ; b School of Pharmacy, Heilongjiang University of Chinese
Medicine, Harbin 150040, PR China
Abstract:
Two new ionone glycosides, named frehmaglutoside G (1) and
frehmaglutoside H (2), together with six known compounds, rehmapicroside
(3), sec-hydroxyaeginetic acid (4), dihydroxy-β-ionone (5),
trihydroxy-β-ionone (6), rehmaionoside A (7) and rehmaionoside C (8) were
isolated from the 95% EtOH extract of the dried roots of Rehmannia glutinosa
Libosch. Their structures were determined on the basis of extensive
spectroscopic analyses, including HR-ESI-MS, UV, IR, 1D and 2D NMR
(1H-1H COSY, HSQC, HMBC, NOESY) methods. The absolute configurations
were confirmed via the circular dichroism (CD) spectra.
Keywords: Rehmannia glutinosa Libosch.; roots; ionone glycosides
*Corresponding author. Email: fwsh@hactcm.edu.cn
Fig.S1 The 1H-NMR spectrum of compound 1
Fig.S2 The 13C-NMR spectrum of compound 1
Fig.S3 The DEPT 135 spectrum of compound 1
Fig.S4 The 1H-1H COSY spectrum of compound 1
Fig.S5 The HSQC spectrum of compound 1
Fig.S6 The HMBC spectrum of compound 1
Fig.S7 The NOESY spectrum of compound 1
Fig.S8 The HR-TOF-MS spectrum of compound 1
Fig.S9 The IR spectrum of compound 1
Fig.S10 The CD spectrum of compound 1
Fig. S11 The 1H NMR spectrum of compound 2
Fig. S12 The 13C NMR spectrum of compound 2
Fig. S13 The DEPT 135 spectrum of compound 2
Fig. S14 The 1H-1H COSY spectrum of compound 2
Fig. S15 The HSQC spectrum of compound 2
Fig. S16 The HMBC spectrum of compound 2
Fig. S17 The NOESY spectrum of compound 2
Fig. S18 The HR-TOF-MS spectrum of compound 2
104.7
100
95
90
85
80
75
806.01
70
897.19
65
1626.79
%T
60
55
927.55
1260.28
1216.40
1452.47
1199.25
1162.06
1370.21
50
45
40
629.64 515.78
578.48
2925.18
35
30
1017.07
3411.36
25
20.6
4000.0
3000
2000
1500
cm-1
Fig. S19 The IR spectrum of compound 2
1000
450.0
Fig. S20 The CD spectrum of compound 2