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Risk assessment
How to weigh out toxic and dangerous heavy metals.
Produced 2013-01-21 By Martin Moche at
Protein Science Facility .
Modified 2013-05-31 By Helena Berglund
Final risk assessment of the method
1. Acceptable risk
1. State the premises in which the
activity is taking place
Scheelelaboratoriet, floor 3,
A1-315
2. Description of activity
How to weigh aliquots of heavy metal salts:
1. Before weighing your compound: Read the material safety data sheet (MSDS) for the compound
of interest.
- If MSDS data sheet classify your chemical as a CMR compound (Carcinogenic, Mutagenic and
Reprotoxic) ask your supervisor/lab manager to be added to the CMR register at PSF.
In MSDS the following risk phrases are used regarding CMR compounds:
H350: May cause cancer.
H340: May cause genetic defects.
H360: May damage fertility or the unborn child.
R45: May cause cancer.
R46: May cause heritable genetic damage.
R49: May cause cancer by inhalation.
R60: May impair fertility.
R61: May cause harm to the unborn child.
- Read the point about handling and storage and take particular note about “incompatible
materials”, MSDS section 10:5, to avoid creating dangerous mixtures of chemicals. If the MSDS
cannot be easily found is next to chemicals and scales - alert the lab manager of the laboratory.
2. Always use the scales in the designated fume hood.
3. Wear lab coat, gloves, eye-protection, long trousers and shoes/boots.
4. Weigh out only the amount you need to use /or that you can weigh out with fair accuracy with
the scales at hand.
5. Use an eppendorf tube (or similar) and a clean metal spatula.
6. Is your compound a fine/dusty powder (like e.g. mersalyl acid), or does it say ‘hygroscopic’ on
the bottle? In that case, the powder may not be easy to transfer into the eppendorf tube, and
would tend to instead stick to the outside of the tube. In those cases, cover first the outside of the
tube (including the rim around the opening) with aluminium foil. This should lessen the problem
considerably.
7. After weighing, wipe the spatula with first a moist and then a dry paper tissue. Then throw the
tissues in the heavy metal bin in the fume hood. The spatula can now be removed from the fume
hood. The powder in the tube should be dissolved in the fume hood before closing the tube and
removing it from the fume hood. The bottle of heavy metal powder can also be retrieved (if
necessary, wipe it first).
8. After the heavy metal solution, heavy metal bottle and spatula have been retrieved; throw away
the foil in the heavy metal bin (if foil was used during weighing), then clean the scales and
surrounding area with first a moist and then a dry paper tissue. Throw away all paper tissues and
finally your gloves in the heavy metal bin.
Risk assessment : Metod för att väga upp giftiga och hälsoskadliga tungmetaller.
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3. Products
Product name
Concentration Form Quantity Danger
Comments
Aluminium fluoride trihydrate
CLP-legislation
H302 , H315 , H319 , H335
P261 , P305 + P351 + P338
Older legislation
R22 , R36/37/38
S26
Lists
Halogenated substance
Biophysics
container
Ammonium heptamolybdate
tetrahydrate
CLP-legislation
H315 , H319 , H335
P261 , P305 + P351 + P338
Older legislation
R36/37/38
S26
Biophysics
container
Barium chloride dihydrate
CLP-legislation
H301 , H332
P301 + P310
Older legislation
R20 , R25
S45
Lists
KI annual use
Biophysics
container
Barium nitrate
CLP-legislation
H272 , H302 , H332
P220
Older legislation
R20/22 , R8
S17 , S28
Biophysics
container
Cadmium Borate
Lists
PRIO Database
Remark M, AFS 2011:18
Remark C, AFS 2011:18
Biophysics
container
Cadmium chloride
CLP-legislation
H301 , H330 , H340 , H350 , H360FD
, H372 , H410
P201 , P260 , P273 , P284 , P301 +
P310 , P310
Older legislation
R25 , R26 , R45 , R46 , R48/23/25 ,
R50/53 , R60 , R61
S45 , S53 , S60 , S61
Lists
PRIO Database
Restricted Substances Database
Chemsec SIN
KI annual use
Halogenated substance
CMR-substances
Remark M, AFS 2011:18
Remark R, AFS 2011:18
Remark C, AFS 2011:18
Cerium(III) sulfate
R36/37/38
S26 , S37/39
Cesium chloride
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Biophysics
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Biophysics
container
Biophysics
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Cesium chloride
container
Cobalt(II) nitrate hexahydrate
CLP-legislation
H317 , H334 , H341 , H350i , H360F ,
H410
P201 , P273 , P280 , P302 + P352 ,
P304 + P341 , P308 + P313
Older legislation
R42/43 , R49 , R50/53 , R60 , R68
S22 , S36/37 , S45 , S53 , S61
Lists
KI annual use
CMR-substances
Remark H, AFS 2011:18
Remark C, AFS 2011:18
Remark S, AFS 2011:18
Biophysics
container
Cobalt(II) sulfate heptahydrate
CLP-legislation
H302 , H317 , H334 , H341 , H350i ,
H360F , H410
P201 , P281 , P302 + P352 , P304 +
P341 , P308 + P313
Older legislation
R22 , R42/43 , R49 , R50/53 , R60 ,
R68
S22 , S35 , S36/37 , S45 , S53 , S61
Lists
KI annual use
CMR-substances
Remark H, AFS 2011:18
Remark C, AFS 2011:18
Remark S, AFS 2011:18
Biophysics
container
Gadolinium(III) nitrate
hexahydrate
CLP-legislation
H272 , H315 , H319 , H335
P220 , P261 , P305 + P351 + P338
Older legislation
R36/37/38 , R8
S17 , S26 , S36/37/39
Biophysics
container
Biophysics
container
Gadolinium(III) oxide
Gallium chloride
Biophysics
container
R14 , R34
S26 , S27 , S28.1 , S36/37/39 , S45
Biophysics
container
Gallium(III) oxide
Gold(III) chloride
CLP-legislation
H315 , H319 , H335
P261 , P305 + P351 + P338
Older legislation
R36/37/38
S26 , S36
Lists
Halogenated substance
mg Salt 250
Uppvägning
av salt
g Salt 25
Uppvägning
av salt
Hexaamminecobalt(III) chloride
CLP-legislation
H315 , H319 , H335
P261 , P305 + P351 + P338
Older legislation
R36/37/38
S26
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Hexachloroplatinic(IV) acid
CLP-legislation
H301 , H314 , H317 , H334
Older legislation
R25 , R34 , R42/43
S1/2 , S22 , S26 , S36/37/39 , S45
Lists
PRIO Database
KI annual use
Biophysics
container
Iridium(III) chloride hydrate
R36/37/38
S26 , S36
Lists
Halogenated substance
Biophysics
container
Biophysics
container
Iridium(III) chloride trihydrate
Manganese(II) chloride
tetrahydrate
CLP-legislation
H302 , H411
P273
Older legislation
R22 , R51/53
S61
Lists
KI annual use
Biophysics
container
Mersalyl acid
CLP-legislation
H300 , H310 , H330 , H373 , H410
P260 , P264 , P273 , P280 , P284 ,
P301 + P310
Older legislation
R26/27/28 , R33 , R50/53
S13 , S28 , S36 , S45 , S60 , S61
Lists
PRIO Database
Restricted Substances Database
KI annual use
Remark H, AFS 2011:18
mg Salt 250
Uppvägning
av salt
Nickel(II) acetate tetrahydrate
CLP-legislation
H302 , H317 , H332 , H334 , H341 ,
H350i , H360 , H372 , H410
P201 , P261 , P273 , P280 , P308 +
P313 , P501
Older legislation
R20/22 , R42/43 , R48/23 , R49 ,
R50/53 , R61 , R68
S45 , S53 , S60 , S61
Lists
Restricted Substances Database
KI annual use
CMR-substances
Biophysics
container
Nickel(II) chloride hexahydrate
CLP-legislation
H301 , H315 , H317 , H331 , H334 ,
H341 , H350i , H360D , H372 , H410
P201 , P261 , P273 , P280 , P301 +
P310 , P311
Older legislation
R23/25 , R38 , R42/43 , R48/23 ,
R49 , R50/53 , R61 , R68
S45 , S53 , S60 , S61
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S45 , S53 , S60 , S61
Lists
Restricted Substances Database
KI annual use
CMR-substances
Remark C, AFS 2011:18
Remark S, AFS 2011:18
p-Chloromercuriphenylsulfonic
acid monosodium
Lists
Restricted Substances Database
Platinum(IV) chloride
R22 , R34 , R42/43
S26 , S27 , S36/37/39 , S45
mg Salt 100
Uppvägning
av salt
mg Salt 1000
Uppvägning
av salt
mg Salt 250
Uppvägning
av salt
mg Salt 1000
Uppvägning
av salt
Potassium dicyanoaurate(I)
CLP-legislation
EUH032 , H300 , H310 , H330 , H410
P260 , P264 , P273 , P280 , P284 ,
P301 + P310
Older legislation
R26/27/28 , R32 , R50/53
S28 , S29 , S45 , S60 , S61 , S7
Lists
PRIO Database
KI annual use
Remark H, AFS 2011:18
Potassium gold(III) chloride
CLP-legislation
H315 , H319 , H335
P261 , P305 + P351 + P338
Older legislation
R36/37/38
S26 , S36
Potassium
hexacyanoferrate(III)
CLP-legislation
EUH032
Older legislation
R32
Lists
Remark H, AFS 2011:18
Biophysics
container
Potassium osmate(VI) dihydrate
CLP-legislation
H301 , H311 , H318 , H330
P260 , P280 , P284 , P302 + P352 ,
P304 + P340 , P305 + P351 + P338 ,
P310
Older legislation
R23/24/25 , R41
S22 , S26 , S36 , S45
Lists
KI annual use
Biophysics
container
Potassium
tetrachloroplatinate(II)
CLP-legislation
H301 , H315 , H317 , H318 , H334
P261 , P280 , P302 + P352 , P304 +
P341 , P305 + P351 + P338 , P309 +
P310
Older legislation
R25 , R38 , R41 , R42/43
S22 , S26 , S36/37/39 , S45
Lists
PRIO Database
KI annual use
Halogenated substance
Risk assessment : Metod för att väga upp giftiga och hälsoskadliga tungmetaller.
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Potassium
tetracyanoplatinate(II)
CLP-legislation
EUH032 , H300 , H310 , H330 , H410
P260 , P264 , P273 , P284 , P301 +
P310
Older legislation
R26/27/28 , R32 , R50/53
S28 , S29 , S45 , S60 , S61 , S7
Lists
KI annual use
mg Salt 1000
Uppvägning
av salt
Biophysics
container
Rhodium(III) chloride
R22
Biophysics
container
Samarium(III) acetate hydrate
Sodium fluoride
CLP-legislation
EUH032 , H301 , H315 , H319
P305 + P351 + P338
Older legislation
R25 , R32 , R36/38
S22 , S36 , S45
Lists
KI annual use
Biophysics
container
Sodium hexachloroiridate(IV)
hexahydrate
CLP-legislation
H301 , H311 , H331
P261 , P280 , P301 + P310 , P311
Older legislation
R23/24/25
S27 , S28 , S36/37/39 , S45
Lists
KI annual use
Biophysics
container
Sodium metavanadate
CLP-legislation
H301 , H315 , H319 , H335
P261 , P301 + P310 , P305 + P351 +
P338
Older legislation
R25 , R36/37/38
S26 , S36/37/39 , S45
Lists
KI annual use
g Salt 25
Uppvägning
av salt
Sodium molybdate
CLP-legislation
H315 , H319 , H332 , H335
P261 , P305 + P351 + P338
Older legislation
R36/37/38
S26 , S36
Sodium molybdate dihydrate
Biophysics
container
Biophysics
container
Sodium sulfide nonahydrate
CLP-legislation
EUH031 , H302 , H311 , H314 , H400
P233 , P273 , P280 , P301 + P330 +
P331 , P305 + P351 + P338 , P310
Older legislation
R31 , R34 , R50
S26 , S29 , S45 , S61
Lists
KI annual use
Risk assessment : Metod för att väga upp giftiga och hälsoskadliga tungmetaller.
Biophysics
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Sodium tungstate dihydrate
CLP-legislation
H302
Older legislation
R22
S36
Biophysics
container
Strontium carbonate
Biophysics
container
Strontium chloride hexahydrate
CLP-legislation
H302 , H318
P280 , P305 + P351 + P338
Older legislation
R22 , R41
S26 , S39
Biophysics
container
Tin(II) chloride
CLP-legislation
H302 , H314 , H315 , H317 , H319 ,
H335
P280 , P302 + P352 , P304 + P340 ,
P305 + P351 + P338 , P310
Older legislation
R22 , R34 , R36/37/38 , R43
S24 , S26 , S36/37/39 , S45
Ytterbium(III) chloride
hexahydrate
R36/37/38
S26 , S36
Own mixtures
Product name Concentration Form Quantity Danger Comments
Hazard statements
EUH031
Contact with acids liberates toxic gas.
EUH032
Contact with acids liberates very toxic gas.
H272
May intensify fire; oxidiser.
H300
Fatal if swallowed.
H301
Toxic if swallowed.
H302
Harmful if swallowed.
H310
Fatal in contact with skin.
H311
Toxic in contact with skin.
H314
Causes severe skin burns and eye damage.
H315
Causes skin irritation.
H317
May cause an allergic skin reaction.
H318
Causes serious eye damage.
H319
Causes serious eye irritation.
H330
Fatal if inhaled.
H331
Toxic if inhaled.
H332
Harmful if inhaled.
H334
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H335
May cause respiratory irritation.
H340
May cause genetic defects .
H341
Suspected of causing genetic defects .
H350
May cause cancer .
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H350i
May cause cancer by inhalation.
H360
May damage fertility or the unborn child .
H360D
May damage the unborn child.
H360F
May damage fertility.
H360FD
May damage fertility. May damage the unborn child.
H372
Causes damage to organs through prolonged or repeated exposure .
H373
May cause damage to organs through prolonged or repeated exposure .
H400
Very toxic to aquatic life.
H410
Very toxic to aquatic life with long lasting effects.
H411
Toxic to aquatic life with long lasting effects.
Precautionary
statements
P201
Obtain special instructions before use.
P220
Keep/Store away from clothing/…/combustible materials. OBSERVE! This precautionary statement has to be
specified by the supplier. See the Material Safety Data Sheet for the exact formulation.
P233
Keep container tightly closed.
P260
Do not breathe dust/fume/gas/mist/vapours/spray. OBSERVE! This precautionary statement has to be
specified by the supplier. See the Material Safety Data Sheet for the exact formulation.
P261
Avoid breathing dust/fume/gas/mist/vapours/spray. OBSERVE! This precautionary statement has to be
specified by the supplier. See the Material Safety Data Sheet for the exact formulation.
P264
Wash … thoroughly after handling. OBSERVE! This precautionary statement has to be specified by the
supplier. See the Material Safety Data Sheet for the exact formulation.
P273
Avoid release to the environment.
P280
Wear protective gloves/protective clothing/eye protection/face protection. OBSERVE! This precautionary
statement has to be specified by the supplier. See the Material Safety Data Sheet for the exact formulation
P281
Use personal protective equipment as required.
P284
Wear respiratory protection. OBSERVE! This precautionary statement has to be specified by the supplier. See
the Material Safety Data Sheet for the exact formulation.
P301 + P310
IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P330 +
P331
IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
P302 + P352
IF ON SKIN: Wash with plenty of soap and water.
P304 + P340
IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
P304 + P341
IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for
breathing.
P305 + P351 +
P338
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to
do. Continue rinsing.
P308 + P313
IF exposed or concerned: Get medical advice/attention.
P309 + P310
IF exposed or if you feel unwell: Immediately call a POISON CENTER or doctor/physician.
P310
Immediately call a POISON CENTER or doctor/physician.
P311
Call a POISON CENTER or doctor/physician.
P501
Dispose of contents/container to … OBSERVE! This precautionary statement has to be specified by the
supplier. See the Material Safety Data Sheet for the exact formulation.
Risk phrases
R14
Reacts violently with water.
R20
Harmful by inhalation.
R20/22
Harmful by inhalation and if swallowed.
R22
Harmful if swallowed.
R23/24/25
Toxic by inhalation, in contact with skin and if swallowed.
R23/25
Toxic by inhalation and if swallowed.
R25
Toxic if swallowed.
R26
Very toxic by inhalation.
R26/27/28
Very toxic by inhalation, in contact with skin and if swallowed.
R31
Contact with acids liberates toxic gas.
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R32
Contact with acids liberates very toxic gas.
R33
Danger of cumulative effects.
R34
Causes burns.
R36/37/38
Irritating to eyes, respiratory system and skin.
R36/38
Irritating to eyes and skin.
R38
Irritating to skin.
R41
Risk of serious damage to eyes.
R42/43
May cause sensitisation by inhalation and skin contact.
R43
May cause sensitisation by skin contact.
R45
May cause cancer.
R46
May cause heritable genetic damage.
R48/23
Toxic: Danger of serious damage to health by prolonged exposure through inhalation.
R48/23/25
Toxic: Danger of serious damage to health by prolonged exposure through inhalation and if swallowed.
R49
May cause cancer by inhalation.
R50
Very toxic to aquatic organisms.
R50/53
Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
R51/53
Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
R60
May impair fertility.
R61
May cause harm to the unborn child.
R68
Possible risk of irreversible effects.
R8
Contact with combustible material may cause fire.
Safety advice
phrases
S1/2
Keep locked up and out of the reach of children
S13
Keep away from food, drink and animal feedingstuffs
S17
Keep away from combustible material
S22
Do not breathe dust
S24
Avoid contact with skin
S26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S27
Take off immediately all contaminated clothing
S28
After contact with skin, wash immediately with plenty of ... (to be specified by the manufacturer)
S28.1
After contact with skin, wash immediately with plenty of water
S29
Do not empty into drain
S35
This material and its container must be disposed of in a safe way
S36
Wear suitable protective clothing
S36/37
Wear suitable protective clothing and gloves
S36/37/39
Wear suitable protective clothing, gloves and eye/face protection
S37/39
Wear suitable gloves and eye/face protection
S39
Wear eye/face protection
S45
In case of accident or if you feel unwell, seek medical advise immediately. Show the label where possible
S53
Avoid exposure - obtain special instructions before use
S60
This material and its container must be disposed of as hazardous waste
S61
Avoid release to the environment. Refer to special instructions/Safety data sheets
S7
Keep container tightly closed
4. Chemicals inherent risk
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a:Very high risk
5. Level of exposure
Low
6. Ventilation
Level of protection 2 - ventilated hood
7. Biological material
Not applicable
8. Comments on Biological material
9. Possible interacting effects and reactions.
10. Comments on Possible interacting effects and reactions
MSDS Section 10:5 Incompatible materials
In Summary:
Do not mix heavy atom solutions with:
Strong oxidizing agents, Strong acids, Strong bases, Ammonia, Amines
The nitrate compunds are oxidising:
- Cobalt(II) nitrate hexahydrate
- Gadolinium(III) nitrate hexahydrate
- Barium Nitrat
and should NOT be mixed with other heavy metal compounds.
Ammonium molybdate should not be mixed with heavy metals.
To avoid mixing of nitrate compounds and Ammonium molybdate with heavy metals in the paper
and plastics waste container - we should make a separate waste container for oxidising
compounds. Alternatively we could throw these compounds.
11. Requirements regarding premises and equipment in premises
Ventilation
12. Comments on Requirements regarding premises and equipment in premises
Fume hood is required for weighting of heavy metal compounds.
13. Specification of storage
Ventilated cabinet
14. Comments to Specification of storage
Next to the fume hood we have a ventilated metal cupboard where heavy metal salts are stored.
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15. Do not store together with
Acid , Base , Alcohol , Oxidizing material , Flammable material
16. Comments on Do not store together with
MSDS Section 7.2 Conditions for safe storage, including any incompatibilities
In Summary:
Store in cool place. Keep container tightly closed in a dry and well-ventilated place.
More specific info for certain compounds:
Light sensitive. Air and moisture sensitive. Handle and store under inert gas:
- Gold(III) chloride
Never allow product to get in contact with water during storage. Do not store near acids:
- Potassium dicyanoaurate(I)
Never allow product to get in contact with water during storage. Do not store near acids. Handle
and store under inert gas. Hygroscopic:
- Potassium tetracyanoplatinate
Store under inert gas. Hygroscopic:
- Gadolinium (III) oxide
- Gallium(III) chloride
- Tin(II) chloride
17. Requirements regarding protective signs
Chemicals
18. Commets on Requirements regarding protective signs
19. Personal protective equipment
Protective glasses , Protective gloves , Protective clothing , Protective shoes
20. Comments on Personal protective equipment
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique
(without touching glove
21. Describe the technical equipment
22. Environment
Not applicable
23. Comments on Environment
24. Waste management
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Chemical waste
25. Comments on Waste management
Paper tissue and gloves contaminated with heavy atoms are placed bucket bins in the fume hood.
There is one bin for mercury paper and gloves and another bin for gold/platinum/lead etc
Small plastic eppendorf/falcon tubes with solutions should remain CLOSED when collected in
plastic flasks with screw stoppers and again mercury is separated out from other heavy metal
solutions.
26. First aid and readiness to accident, fire and overflow
Eyewash , Absorbing substance , Other
27. Comments on First aid and readiness to accident, fire and overflow
Instructions for chemical spill cleaning from Stena, full-face filter mask for air protection and a
complete suit protecting against chemicals, vermiculit absorbant, gloves, boots etc. are available in
the end of the corridor at PSF floor 3.
Spill of 1-100 gram chemicals can be handled using the protective equipment above.
In case of an accident please contact Martin Moche or Helena Berglund at:
Martin.Moche@ki.se, 073-3229327, 08-52486843
Helena.Berglund@ki.se, 070-6014289, 08-52486843
28. Hazardous actions
Transference betw vessels , Blending , Weighing , Ventilation failure , Solitary work , Night work
29. Comments on Hazardous actions
Weighing of heavy metals should be done daytime in case of an accident where help is needed.
30. Special instructions to other personel
31. Final risk assessment of the method
1. Acceptable risk
32. Comments on Final risk assessment and additional risk reducing measurements
33. Participants in the assessment
Esben Quistgaard (MBB/Biophysics) wrote the
"Desription of activity" while Martin Moche (MBB/PSF) translated and made the KLARA
documentation.
Signature
Supervisor
Date
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Helena Berglund
Date of reassessment:
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