13.1 Ecologists Study Relationships PHOTOSYNTHESIS 4.2 Overview of Photosynthesis KEY CONCEPT Photosynthesis is a series of chemical reactions that transform light energy into chemical energy stored in food. 4.2 Overview of Photosynthesis Chemical Equation of Photosynthesis 6CO2 + 6H2O Carbon dioxide + Water REACTANTS Carbon dioxide Water Sunlight Energy chlorophyll Sunlight Energy chlorophyll C6H12O6 + 6O2 Glucose + Oxygen PRODUCTS Glucose (sugar) Oxygen 4.2 Overview of Photosynthesis 4.2 Overview of Photosynthesis CO2 diffuses into leaf cells through stomata pores 4.2 Overview of Photosynthesis Water is absorbed by osmosis through the roots 4.2 Overview of Photosynthesis In plants, Photosynthesis occurs in chloroplasts. chloroplast • Chloroplasts are green colored organelles that contain the green pigmented molecule chlorophyll leaf cell •Chloroplast = Organelle •Chlorophyll= Molecule leaf 4.2 Overview of Photosynthesis Structure of the Chloroplast Organelle 1.) Thylakoids- coin shaped compartments surrounded by a membrane chloroplast grana (thylakoids) 2.) Grana – Stacks of coin shaped thylakoids 3.) Chlorophyll – light absorbing green molecule in thylakoid membrane 4.) Stroma – Fluid filled space between grana stroma 4.2 Overview of Photosynthesis There are Two Main Stages of Photosynthesis 1. Light-Dependent Reactions – Require Light Energy 2. Light-Independent Reactions – Do Not Require Light Energy 4.2 Overview of Photosynthesis • Light-Dependent Reactions - capture energy from sunlight. *Light energy is needed *Water is needed *Occur in the chlorophyll filled thylakoid membrane *Produce molecules that provide E to Light Independent Rxns. (ATP and NADPH – High Energy Molecules) *Waste byproduct is Oxygen 4.2 Overview of Photosynthesis Basic Steps of the Light Dependent Reactions 1. Light energy “excites” the electrons in the chlorophyll molecule. 2. “Excited”, high energy electrons are passed from one protein molecule to another along the electron transport chain in the thylakoid membrane and are picked up by two molecules: NADP to form NADPH High E molecules needed to ADP to form ATP power the Light Independent Rxns 3. Enzymes split the water molecule: H2O 2H+ + 2e- + O* The 2e- replace the electrons the chlorophyll molecule lost * Oxygen gas is released 4.2 Overview of Photosynthesis • Light-Independent Reactions (Calvin Cycle) • • • • • Do NOT require light energy – can happen in the dark Requires ATP and NADPH as energy source Occurs in the stroma CO2 needed. CO2 enters through the leaf stomata by diffusion Glucose molecules made 4.2 Overview of Photosynthesis Basic Steps of the Light Independent Reactions (Calvin Cycle) 1. CO2 molecules diffuse across the stroma. 2. CO2 is rearranged using H+ and Energy from ATP and NADPH to make glucose. Carbon gets ”fixed” into organic compounds – Carbohydrates, Lipids, Proteins 4.2 Overview of Photosynthesis • Light Dependent Reactions – Capture Light Energy • Light Independent Reactions - Make Sugars 4.2 Overview of Photosynthesis • The equation for the overall process is: 6CO2 + 6H2O C6H12O6 + 6O2 granum (stack of thylakoids) 1 chloroplast 6H2O thylakoid 6CO2 3 6O2 2 energy stroma (fluid outside the thylakoids) 1 six-carbon sugar 4 C6H12O6