Photosynthesis An introduction Class Brainstorm Photosynthesis The 5 Ws of Photosynthesis Who? What? Chloroplasts When? Use light energy and CO2 to make sugars Where? Photoautotrophs Need light Why? Source of energy for other organisms Chlorophyll Definition: A green colored pigment that absorbs light Two types: Chlorophyll a (blue-green) and Chlorophyll b (yellow-green) Consists of a porphyrin ring and a long hydrocarbon tail See page 139 for image Porphyrin ring: a Mg atom at the centre and a hydrocarbon ring of alternating single and double bonds Chlorophyll a has a methyl group (-CH3) Chlorophyll b has an aldehyde group (-COH) Hydrocarbon tail or Phytol tail: anchors molecule to a membrane Eukaryotic Autotrophs Algae, Photosynthetic Protists, and Plants Chlorophyll in organelles: chloroplasts Chloroplasts found in leaves (gives them green color) Leaf Structure Waxy cuticle Upper Epidermis Palisade Mesophyll Spongy Mesophyll Mesophyll Vascular Bundle Lower Epidermis Guard Cells Chloroplast: Photosynthetic “Factories” Sketch and label a chloroplast Inner and outer membrane Stroma – protein-rich semi-liquid Thylakoids – membrane bound sacs contain chlorophyll and ETC Grana – stacks of thylakoids Lamellae – unstacked thylakoids that connect grana See page 144 Photosynthesis Overview Step 1. Capture light energy. Step 2. Make ATP and reduced NADP+ Step 3. Make glucose and organic compounds. Light Electromagnetic radiation (EM): light is a form of energy Visible light 380 (violet) -750 (red) nm Travels in wave packets called photons Absorption Spectrum: the wavelengths of light that a pigment will absorb Action Spectrum: the most effective wavelength for photosynthesis See page 152 Read “A Brief History of Photosynthesis Research” on page 149150. What experiment did J.B. Van Helmont conduct? What did he conclude? What experiment did Joseph Priestly conduct? What did he conclude? What is the source of O2 in photosynthesis? Hmwk Prelab for Fermentation Lab Study for Quiz Hmwk (take-up on Tues) Qs: p145 #5b, 7, 9 Qs: p154 #1, 3, 6, 7, 8