The overall aim of this Project
Development luminescent
lanthanide nanobioprobes for
detection of
N-acetyl aspartate(NAA) .
EX OF N-Acetyl Amino acids
N-acetyl aspartate(NAA)
C6H9NO5
HO
O
O
HO
O
N
H
The interest in N-acetylaspartate determination is due to it present
in high concentration in the brain and their possible direct impact
on the human as :
Decreased levels of NAA
1- Alzheimer’s disease
2- AIDS
3- schizophrenia
4- Huntington’s disease
5- amyotrophic lateral or multiple sclerosis
6- traumatic brain injury
high levels of NAA :
causes Canavan’s disease
1-Magnetic resonance spectroscopy
The major advantage of 'H NMR spectroscopy is that this
technique can be applied to in vivo studies of brain
The major disadvantage of NMR is its low sensitivity, allowing
only metabolites present at relatively high concentration to be
measured.
2-Chromatographic techniques
HPLC , Gas and Liquid chromatography
Advantages of HPLC
•Sensitivity
•Accuracy
•Automation
•High resolution
Disadvantages of HPLC
•Complexity
• The analysis time is long
•A further principal drawback of these methods is the requirement of
(sometimes harmful) organic solvents of HPLC grade quality, whereby the
costs for their purchase and disposal has to be taken into consideration
development of an inexpensive, fast and
sensitive method for the determination of NAA is of primary interest .
One of the most convenient and simplest detection method is based on
the change in absorption or luminescence in the presence of an analyte.
The lanthanide complexes, with characteristically narrow excitation
and emission bands, intense luminescence, and long excited-state
lifetimes, have drawn considerable interest for their potential application
as luminescence imaging agents and sensors.
Luminescence is a highly sensitive analytical method nowadays, its
interest for medical analysis and diagnosis.