LIPIDS To be able to • Identify the elements which make up lipids • Explain that glycerol and fatty acids combine by condensation to produce triglycerides. • Demonstrate why the R group of a fatty acid may be saturated or unsaturated. • Explain how phospholipids are formed LIPIDS • • • • What are lipids? Why are they useful? What is their biological use? What elements make up lipids? TRIGLYCERIDES • The most common type of lipids are the triglycerides also known as fats and oils, or TRIGLYCERIDES • Triglycerides are made from a combination of three fatty acid molecules and one glycerol molecule by a condensation reaction. Fatty Acid Fatty Acid Fatty Acid Glycerol The glycerol molecule in any triglyceride is always the same. It is the fatty acids that vary Glycerol is a small, 3-carbon molecule with three alcohol groups. H H H H C C C OH OH OH H Fatty acids are long molecules with a polar, hydrophilic end and a nonpolar, hydrophobic "tail". H H H H H O H C H Methyl Group C C H H C C H H Hydrocarbon Chain (14-22 carbon atoms) C OH Carboxyl Group The hydrocarbon chain can be from 14 to 22 CH2 units long, but it is always an even number because of the way fatty acids are made.. The hydrocarbon chain is sometimes called an R group, so the formula of a fatty acid can be written as R-COO- General formula CH3 – (CH2)n - COOH Saturated fatty acids • Saturated fatty acids only have C-C bonds • Each carbon in the chain is joined by a single bond COO • Each carbon is SATURATED with hydrogen • These fatty acids form straight chains, and have a high melting points. Unsaturated fatty acids • Unsaturated fatty acids only at least one C=C double bond • Polyunsaturates have more than one double bond H H H H H H H H H C C C C C C C C H H H H H H COO- • These fatty acids form bent chains, and have a low melting point. Formation of triglycerides • One molecule of glycerol joins together with three fatty acid molecules to form a triglyceride molecule, in another condensation polymerisation reaction: H H H H H H H C C C C C C C H H H H H H H H H H H H H C C C C C C C H H H H H H H H H H H H H C C C C C C C H H H H H H O OH O OH O OH H2O H HO C H H2O HO C H H2O HO C H H H CH3 – (CH2)n - COOH HO C CH3 – (CH2)n - COOH HO C H CH3 – (CH2)n - COOH HO C H = O CH3 – (CH2)n - C H 3H2O H O C H ESTER BONDS = = O CH3 – (CH2)n - C O CH3 – (CH2)n - C H O C H O C H H Phospholipids •Phospholipids have a similar structure to triglycerides, but with a phosphate group in place of one fatty acid chain. •There may also be other groups attached to the phosphate. Phospholipids have a polar hydrophilic "head" (the negatively-charged phosphate group) and two non-polar hydrophobic "tails" (the fatty acid chains). O- H H C O = O CH3 – (CH2)n - C O C H = O CH3 – (CH2)n - C O C H H P= O O- This mixture of properties is fundamental to biology, for phospholipids are the main components of cell membranes. water Steroids Steroids are small hydrophobic molecules found mainly in animals. They include: •Cholesterol, which is found in animals cell membranes to increase stiffness •Bile salts, which help to emulsify dietary fats •Steroid hormones such as testosterone, oestrogen, progesterone and cortisol •Vitamin D, which aids Ca2+ uptake by bones. • Why is it wrong to call lipids ‘fats’? • Because saturated lipids are mainly animal fats but unsaturated lipids are called oils • What is the name given to the type of bond that joins fatty acids and glycerol in a lipid molecule? • Ester bond • Why are lipids insoluble in water? • Because they have a hydrophobic tail that repels water and this causes the molecules to clump together to reduce the surface area in contact with water. • Describe the difference between a triglyceride and a phospholipid. How do these differences affect the properties of the molecule? • A phosphate group replaces one of the fatty acid molecules • The phosphate group is ionised which makes it attract water molecules. This end is therefore hydrophilic as opposed to the fatty acid group which is hydrophobic