Chemistry 125: Lecture 45 January 28, 2011 This Nucleophilic Substitution and Mechanistic Tools: Solvent, Leaving Group PET Scanning Pentavalent Carbon? For copyright notice see final page of this file SN2 Nucleophilic Substitution Solvent Nu: R-L Nucleophile Substrate (+) (-) Nu-R L Product Leaving Group the Pragmatic Logic of Proving a Mechanism with Experiment & Theory (mostly by disproving all alternative mechanisms) Rate Constant Dependance on Nucleophile Nu R-L Leaving Substrate Group krel pKa [1] -1.7 F- 80 3.2 Cl- 1,000 -8 Br- 10,000 -9 HO- 16,000 15.7 I- 80,000 -10 HS- 126,000 7 Nu-R L Polar solvents accelerate reactions that generate (or concentrate) charge, and vice versa. (NuH+) krel CH3I in H2O harder [1] to break 14 H-bonds to smaller ions 160 krel CH3Br in Acetone Backwards H2O (-) (+) 11 5 Sensible Nu: Solvent [1] e.g. J&F Sec. 7.4dg Rate Constant Dependance on Nu: Nucleophile Solvent R-L Leaving Substrate Group L (+) Nu-R (-) L pKa (LH+) HO- v. bad 15.7 HS- bad 7 FH2O bad good 3.2 -1.7 RSO2O- good -3 Cl- good -8 BrI- good v. good -9 -10 Weak bases are good leaving groups (Stable anions form easily. Those that don’t hold tightly to H+ don’t hold tightly to Nud- C in the NudC LdSN2 transition state, as expected) e.g. J&F Sec. 7.4e How? With permission of the Edmund S. Muskie Archives and Special Collections Library – Bates College Molecule specifically designed and prepared to test these mechanistic questions Cl Lawrence H. Knox Paul D. Bartlett (1908-1964) (1907-1997) Bartlett and Knox * (J.Am.Chem.Soc. - 1939) Need a Fabulous Leaving Group! O Cl now an “allylic” N H + Nrearrangement shifts H2O HO H O 2 H from N to O N pKa + Cl H + NH2 H-NH2 = 34 Can generate even + H-N+H3 = 9 bridgehead cation! + Near the end of the semester we’ll discuss R-COOH R-CNH2 R-NH2 How? Molecule specifically designed and prepared to test these mechanistic questions Cl Bartlett and Knox * (J.Am.Chem.Soc. - 1939) Rate Constant Dependance on Nu: Nucleophile Solvent R-L Leaving Substrate Group L (-) (+) Nu-R L pKa (LH+) HO- v. bad 15.7 HS- bad 7 FH2O bad good 3.2 -1.7 RSO2O- good -3 Cl- good -8 BrI- good v. good -9 -10 Weak bases are good leaving groups (H like R, as expected) R-OH v. bad R-OH2+ good (acid catalysis) R-OSO2R’ good (Kenyon/Phillips) e.g. J&F Sec. 7.4e OH Leaving-Group-Trick Lore (e.g. J&F sec 7.4f) OH2+ BrCH3-CH2-OH Br CH3-CH2 + OH- Bad leaving group Br CH3-CH2 + OH2 H-O+H2 pKa -1.7 Good leaving group H-OH pKa 16 H-Br pKa -5 Br- CH3-CH2-O+H2 Ether Cleavage by HBr Br- excess 47% HBr O Br 8 hr Br + O Good Leaving Group OH H Br Good Nucleophile OH Leaving-Group-Trick Lore (e.g. J&F sec 7.4f) OSO2R O H PhCH2 CH CH3 Cl SO2 H OSO2 CH3 CH3 toluenesulfonic acid pKa -3 Kenyon & Phillips (1923) O SO2 PhCH2 CH CH3 CH3 “tosylate” O O C CH3 O O C CH3 PhCH2 CH CH3 OH Leaving-Group-Trick Lore (e.g. J&F sec 7.4f) OSOCl b.p. 75°C 61°C gases OH Leaving-Group-Trick Lore (e.g. J&F sec 7.4f) OPXn (CH3)2CHCH2OH PBr3 (CH3)2CHCH2Br + P(OH)3 (58%) -10°C, 4 hr Larger rings allow flattening Inaccessible for SN2 of bridgehead cation. PCl5 + OH PClx CaCO3 ether 0°C, 3 min Substitution of RO- for Cl- at P (probably A/D mechanism) generates good leaving group. Cl (“100%”) OH Leaving-Group-Trick Lore (e.g. J&F sec 7.4f) Appel Reaction + OP Ph3 (Wikipedia) pKa ~17 Substitution of P for CCl3 at Cl pKa 24 A/D substitution at P (vacant d-orbitals) H e.g. D D OH Cl f3P CCl4 25°C, 24 hr H D D (~85%) Using SN2 Mechanistic Knowledge to Maximize Synthetic Speed for PET scanning (from Loudon, Org. Chem.) http://en.wikipedia.org/wiki/Positron_emission_tomography Connecting simultaneous scintillations shows where 18F’s were. 18O= + 7 MeV proton - neutron 18F- or 11C t ~ 20 min 1/2 13N t ~ 10 min 1/2 15O t ~ 2 min 1/2 t1/2 110 min +e positron +18O= proton neutron Need to get 18F where tumor is and you have to do so within a few hours of preparing the element. Yale PET What to synthesize? Protected Triflate to 2-Fluoroglucose - ASAP Glucose 2-Fluoroglucose KF18 SN2 ? F18 and by K+cation would suck up 18F Maybe tied upitby H-bonding 2-Fluoroglucose as well. HO a horrid leaving group trifluormethanesulfonate wrong C-OH could be attacked (Triflate) “protection” is Now to introduce 18F sugar chemistrySN2 inversion gives wrong configuration start with Rapid metabolism ofMannose tumor sucks up glucose. SN2 Problems : This kind of well known in pKa ~ -14 Cl-SO2CF3 O AcO = CH3C-O- (acetate protecting group) Protected Triflate to 2-Fluoroglucose - ASAP Glucose 2-Fluoroglucose KF18 SN2 “deprotection” H2O H+ and by K+cation 18F tied up by H-bonding K+ K+ 18FCH3CN (aprotic solvent) Cl-SO2CF3 Vertical Section viewed from front PET Scan Image measured 1 hour after administering fludeoxyglucose (18F) shows high glucose metabolism in brain and in a cancerous lymph node. Horizontal Section viewed from beneath Linus Pauling 1901-1994 Akira Kouchiyama Tools for Testing (i.e. Excluding) Mechanisms: Stereochemistry Rate Law Rate Constant Structure X-Ray and Quantum Mechanics So far we’ve just been beating up on the D/A mechanism (trivalent C intermediate) though there are cases (SN1) where it in fact applies. The tougher problem is to distinguish between concerted and A/D with a very weakly stabilized intermediate. (see supplementary reading on Course website) Might there be Pentavalent A/D Intermediate instead of a Concerted SN2 Transition State? Nu C L Transition State Nu C L Pentavalent Intermediate Might there be Pentavalent A/D Intermediate instead of a Concerted SN2 Transition State? 2.64 Å 2.64 Å Quantum Mechanics says Transition State for H2O attacking protonated t-BuOH. 1.88 Å 1.88 Å Quantum Mechanics says Transition State for OH- attacking less crowded CH3OH. But neither reaction is practical in the laboratory! What does experiment say? X-ray? End of Lecture 45 Jan. 28, 2011 Copyright © J. M. McBride 2011. Some rights reserved. Except for cited third-party materials, and those used by visiting speakers, all content is licensed under a Creative Commons License (Attribution-NonCommercial-ShareAlike 3.0). Use of this content constitutes your acceptance of the noted license and the terms and conditions of use. Materials from Wikimedia Commons are denoted by the symbol . Third party materials may be subject to additional intellectual property notices, information, or restrictions. The following attribution may be used when reusing material that is not identified as third-party content: J. M. McBride, Chem 125. License: Creative Commons BY-NC-SA 3.0