VITAMIN C CONCENTRATION
IN DIFFERENT ORANGE JUICE
BRANDS
INTRODUCTION
Vitamin c also known as ascorbic acid is one
of the most effective nutrients. Benefits
vitamin c may include protection against
immune system deficiency, cardiovascular
disease, prenatal health problems eye
disease and even skin wrinkling. Vitamin c is
a water soluble vitamin meaning it is not
stored in our bodies. We have to get vitamin
c from foods like citrus fruits, broccoli, and
tomatoes.
Vitamin C reacts with DCPIP in the following
manner:
DCPIP (blue) + H+ ——→ DCPIPH (pink)
DCPIPH (pink) + VitC ——→ DCPIPH2 (colorless)
Overall Reaction:
C6H8O6 + C12H7NCl2O2 ——→C6H6O6 + C12H9NCl2O2
Ascorbic acid + HDCPIP ─→ dehydroascorbic acid + DCPIPH2
VARIABLES
Independent Variable Different orange fruit
juices
Dependent Variable Titre value of fruit juices
Controlled Variable Concentration and amount
of DCPIP
OUR EXPERIMENT
AIM
To compare the vitamin C content in:
· Home-made squeezed orange juice
· Real orange fruit juice,
·Tropicana - orange fruit juice, and
·Minute maid pulpy orange juice.
Thereby determining which of the above is best for
consumption of Vitamin C.
The vitamin c content would be the greatest in the
homemade orange juice from orange fruit when
compared to market juices.
Materials Used
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Containers to collect small amount of juices
Measuring cylinder
Pipette to measure 2 ml volume
Pipette to accurately measure volume up to 1 ml
Test tubes (5)
Spatula
Distilled water
Vitamin C (0.1% fresh: 0.1% = 1mg /ml)
DCPIP dye (0.01% aqueous solution fresh)
Procedure
•Wash all the apparatus thoroughly with water.
•Prepare orange juice from orange fruit without
adding any water to it.
•Take 2ml of 0.01% DCPIP solution in a testube.
•Take juice in 1ml pipette and titrate it against
2ml DCPIP solution.
•Shake the tube gently after the addition of each
drop and continue to add drops until the DCPIP
solution is decolorized.
•Repeat this process for all other juices.
PRECAUTIONS
• The titration has to be drop by drop as the
solution may change within the addition of
amounts as small as two drops.
• The concentration of the DCPIP has to be kept
constant, so the amount of dye to be added has
to be of precise value
• The apparatus has to be clean or else the same
apparatus shouldn’t be used for keeping the
juices because it wont give the correct readings.
RAW DATA
• Freshly Squeezed Orange Fruit Extract
1
2
3
Average
0.04ml
0.05ml
0.05ml
0.04ml
Result
The colour change after titarion
• Real Orange Juice
1
2
3
Average
0.34ml
0.26 ml
0.26ml
0.28ml
Result
The colour change after titration
• Tropicana Orange Juice
1
2
3
Average
0.33
0.35
0.35
0.34
result
Colour change after titration
• Minute Maid Pulpy Orange Juice
1
2
3
Average
0.55ml
0.60ml
0.60ml
0.58ml
Result
Colour change after titration
• 0.1% Ascorbic Acid
1
2
3
Average
0.06ml
0.04ml
0.04ml
0.04ml
Result
Colour Change after Titration
DATA PROCESSING
Graph showing vitamin c concentration
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
hm
real
tropicana
mm
as.acid
Error graph
sr
hm
1
2
3
hm
av
max-mean
mean-min
tropicana real
0.04
0.33
0.05
0.35
0.05
0.35
tropicana real
0.046
0.343
0.01
0.01
0.006
0.013
minute maid as.acid
0.34
0.55
0.26
0.6
0.26
0.6
minute maid as.acid
0.28
0.58
0.06
0.02
0.02
0.03
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
hm
tropicana
real
minute maid
as.acid
0.06
0.04
0.04
0.04
0.02
0
sr
Homemade
1
2
3
Tropicana
0.04
0.05
0.05
Homemade
av
stdev
0.33
0.35
0.35
Tropicana
0.04
0.005774
0.34
0.011547
Standard deviation for homemade and Tropicana
0.4
0.35
0.3
0.25
Volume(ml)
0.2
0.15
0.1
0.05
0
hm
tropicana
juices
sr no.
hm
average
minute maid
1
0.04
0.55
2
0.05
0.6
3
0.05
0.6
hm
mm
stdev
0.04
0.58
0.01
0.03
Standard deviation for homemade and
minute maid
0.7
0.6
0.5
0.4
volume(ml)
0.3
0.2
0.1
0
hm
juices
mm
Std. deviation for Freshly Squeezed
juice & Tropicana
sr no.
hm
real
1
2
3
juices
average
stdev
0.04
0.05
0.05
Home made
0.34
0.26
0.26
real
0.04
0.01
0.4
0.3
Volume(ml)
0.2
0.1
0
hm
tropicana
juices
0.28
0.0462
LIMITATIONS
• The most difficult part of the experiment was
detecting the change in colour of the DCPIP
solution while performing the titration. Two of
the juices showed clear disappearance of the
colour, while the others had the tint of yellow
due to some other compounds of colours
present.
• The date of manufacture may be different which
may change the nutrient content or
concentration in different juices.
Conclusion
From our experiment we thus
conclude that homemade juice has
the most vitamin c concentration
when compared to available market
juices.
THANK YOU…
Compiled & Presented By:
Vibhuti Sapariya
Niyati Bamrolia