AP BIOLOGY Review Campbell Chapter 7 Plants that can tolerate extreme cold have a higher percentage of phospholipids with unsaturated ______________ fatty acid tails. SATURATED UNSATURATED Cholesterol can be called a “temperature buffer” because at higher temperatures (like body temp) it less fluid by restraining makes the phospholipids ________ their movements. less more At lower temperatures cholesterol hinders the close packing of phospholipids so it __________ lowers the temperature required for membranes to solidify. lowers raises Proteins that penetrate the hydrophobic core of Integral proteins a membrane are called _________________ Membrane proteins with carbohydrates attached are called ____________________ glycoproteins Living things must control the balance of water and ions in their tissues. This is called Osmoregulation (kind of homeostasis) _____________________ http://www.microscopy-uk.org.uk/mag/imgjun99/vidjun1.gif If a Paramecium were to swim from a hypotonic environment to an isotonic one, would the activity of its contractile vacuole increase or decrease? Campbell Concept check 7.3 It would decrease, because less water would be entering An environment in which the concentration of solutes outside and inside the cell are equal is isotonic called _________________ An animal cell placed in this type of environment will _____________________ stay the same size swell & burst shrink stay the same size The diffusion of water across a semi-permeable osmosis membrane is called _________________ An environment in which the concentration of solutes is greater outside the cell than inside is called _________________ hypertonic An animal cell placed in this type of environment will _____________________ shrink swell & burst shrink stay the same size Membrane transport that requires energy is called ACTIVE ________________ The Na+-K+ pump in animals causes the cytoplasm inside cells to be more ___________ negative than the surrounding extracellular fluid negative positive Membrane lipids with carbohydrates attached are called ____________________ glycolipids In passive transport molecules always move DOWN ___________ their concentration gradient. down up An environment in which the concentration of solutes outside is less than inside the cell is hypotonic called _________________ An animal cell placed in this type of environment swell and burst will _____________________ swell & burst shrink stay the same size Channel proteins that facilitate the movement of water across cell membranes are called aquaporins ___________________ channel Transport proteins called _____________ proteins function by forminng a hydrophilic tunnel that allows charged molecules or ions to pass through the hydrophobic portion of a cell membrane. carrier Transport proteins called _____________ proteins hold on to their passenger molecules, change shape, and shuttle them across the membrane. As a cell grows, its plasma membrane expands Exocytosis; when vesicles fuse with membrane via ___________________. endocytosis exocytosis they become part of the membrane Campbell Concept check 7.5 Name 3 kinds of passive transport Osmosis, diffusion, facilitated diffusion Name 3 kinds of active transport Na+-K+ pump, co-transport, bulk transport, endocytosis, pinocytosis, phagocytosis, receptor mediated endocytosis, exocytosis Ion channels that open or close in response to a gated signal are called ___________________ Name the kind of transport Facilitated diffusion with an Ion channel Name the kind of transport Facilitated diffusion with a carrier A transport protein that generates voltage across a membrane is called an ________________________ Electrogenic pump When nerve cells establish a voltage across their membrane with a sodium-potassium pump, does this pump use ATP or produce ATP? Campbell Concept check The pump uses ATP. To establish a voltage, ions would have to be pumped against their gradient, which requires energy http://www.biologymad.com/NervousSystem/nakpump.gif Name this kind of transport Sodium-potassium pump Give an example of bulk transport Endocytosis (phagocytosis, pinocytosis, receptor mediated endocytosis) Exocytosis Animation from: http://academic.brooklyn.cuny.edu/biology/bio4fv/page/cell-movement.html Name this kind of transport used by bacteria to take in food or white blood cells to engulf bacteria phagocytosis http://www.accs.net/users/kriel/chapter%20nine/ If this vesicle is releasing products outside the cell, this type of transport is called exocytosis ________________________ If this vesicle is bringing small molecules & fluids into the cell, this type of transport is called ______________________ pinocytosis http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBooktransp.html#Vesicle-mediated%20transport Animation from: http://www.sp.uconn.edu/~terry/images/anim/fluidmem.gif Click here to See Fluidity The currently accepted model that describes cell membranes as the arrangement of constantly moving proteins embedded in or attached to a bilayer or phospholipids is called the Fluid mosaic model ______________________________________ http://academic.brooklyn.cuny.edu/biology/bio4fv/page/rectpr.htm Name this kind of transport that enables the cell to acquire bulk quantities of specific substances Receptor mediated endocytosis The molecules that bind to these receptors are ligands called _____________________ Give an example of a molecule that moves into Cholesterol cells in this way _________________ LDL ligands bind receptors and attached cholesterol is taken into cell Name the kind of transport that moves molecules from high concentration to low across a membrane without using energy and without the help of any carriers or vesicles DIFFUSION To send a signal, a neuron may carry out exocytosis of chemical signals that are recognized by a 2nd neuron. In some cases, the 1st neuron ends the signal by taking up the signaling molecules by endocytosis. Would you expect this to occur by pinocytosis or by receptor mediated endocytosis? EXPLAIN Campbell Concept check Receptor mediated endocytosis because in this case one specific molecule needs to be taken up at a particular time; pinocytosis takes up molecules in a non-specific manner http://www.uic.edu/classes/bios/bios100/lecturesf04am/sucrosepump.jpg Name this kind of transport in which transport of one substance DOWN its gradient is coupled to the movement of another moving UP the gradient. cotransport http://www.biologymad.com/NervousSystem/nakpump.gif Explain why the sodium-potassium pump is NOT considered a cotransporter. Campbell concept check 7.4 Each ion is being transported against its gradient. If either ion were flowing DOWN its electrochemical gradient, this would be Co-transport. Co-transport couples the movement of one substance down its gradient with one moving up. Name the kind of transport used by Golgi bodies in secretory cells to export their products. exocytosis Name the kind of transport used by cholesterol to enter cells Receptor mediated endocytosis Name the molecule that provides energy for active transport ATP http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBooktransp.html#Vesicle-mediated%20transport The swelling and bursting of animal cells when placed in a cytolysis hypotonic environment is called _________________ The shrinking and shriveling up of animal cells when placed in a hypertonic environment is called ___________________ crenation http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBooktransp.html#Vesicle-mediated%20transport The swelling of plant cells when placed in a hypotonic increase environment ______________ the osmotic pressure inside the cell increases decreases turgid We would say the cell is ___________ turgid flaccid http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBooktransp.html#Vesicle-mediated%20transport The pulling away of the cell membrane from the cell wall in plant cells when placed in a hypertonic environment is plasmolysis called _______________________ A plant cell that has lost water and is “limp” is called _____________________ flaccid Carrier proteins like this one are ______________proteins. integral Integral peripheral Carbon dioxide and oxygen move across membranes in cells by Diffusion _________________ Kind of endocytosis used to take in large particles or whole cells. phagocytosis The voltage across a membrane is called __________________ membrane potential When you sit in the bathtub, your fingers get wrinkly because of the water entering your skin cells. The bathtub water is a ____________ hypotonic solution compared to your skin cells Hypotonic isotonic hypertonic A membrane that lets certain molecules pass through and not others is called _______________ Semi permeable OR selectively permeable Name a kind of transport that uses vesicles to move substances across a membrane Pinocytosis, phagocytosis, Exocytosis, endocytosis Name the kind of transport that moves WATER across cell membranes OSMOSIS A freshwater fish has about 1% salt in his body. Freshwater is close to 0% salt. Will water move into or out of this kind of fish? More solute molecules inside the fish’s cells than in the freshwater. (HYPOTONIC) Water will move INTO the fish Which of the following factors would tend to increase membrane fluidity? A A. a greater proportion of unsaturated phospholipids B. a greater proportion of saturated phospholipids C. a lower temperature D. a relatively high protein content in the membrane E. a greater proportion of relatively large glycolipids compared to lipids having smaller molecular masses Campbell Concept check Which organelle makes the ATP used to run the Na + K+ pump? mitochondria Type of endocytosis in which cells take in small molecules or fluids pinocytosis The white circles stand for oxygen molecules. Use what you know about diffusion of molecules to predict which way the oxygen will move. Diffusion moves molecules from high concentration to low so… from the lungs into the blood Based on the model of sucrose uptake in the figure below, which of the following experimental treatments would increase the rate of sucrose transport into the cell? A. decreasing extracellular sucrose concentration B B. increasing extracellular pH C. decreasing cytoplasmic pH D. adding an inhibitor that block the regeneration of ATP E. adding a substance that make the membrane more permeable to hydrogen ions Campbell Concept check This type of transport which opens in response to a signal and forms a “tunnel” through the membrane to let ions in and out is called a(n) ___________________ Gated ion channel Tell if the transport is ACTIVE or PASSIVE PASSIVE Facilitated diffusion ___________________ Osmosis ____________________ PASSIVE Na + - K+ pump ____________________ ACTIVE Diffusion ____________________ PASSIVE Endocytosis _______________________ ACTIVE ACTIVE Exocytosis ________________________ Ion channels ________________________ PASSIVE Proton pumps ______________________ ACTIVE Aquaporins _____________________ PASSIVE Match the picture with the kind of transport FACILITATED DIFFUSION (carrier) DIFFUSION Match the picture with the kind of transport EXOCYTOSIS INSIDE CELL OUTSIDE CELL Substance is put in a vesicle and transported up to the cell membrane and released OUTSIDE Name an organelle that uses this kind of transport Golgi Bodies In what way do the various membranes of a eukaryotic cell differ? A. Phospholipids are found only in certain membranes B B. Certain proteins are unique to each membrane C. Only certain membranes of the cell are selectively permeable. D. Only certain membranes are constructed from amphipathic molecules E. Some membranes have hydrophobic surfaces exposed to the cytoplasm; while others have hydrophilic surfaces facing the cytoplasm. Campbell Concept check Match the picture with the kind of transport Na+ - K + pump Energy from ATP is used to move 3 Na + ions OUT of the cell and carry 2 K + ions INTO the cell This pump is the main electrogenic pump used in ______________ cells. animal plant animal Which of the following processes includes all the others? A. osmosis B. diffusion of a solute across a membrane C. facilitated diffusion D. passive transport E. transport of an ion down its electrochemical gradient D; diffusion, osmosis, facilitated diffusion, ion channels are all kinds of passive transport Campbell Concept check Match the picture with the kind of transport ENDOCYTOSIS Substance is transported INTO cell inside a vesicle If what is moving into the cell is a small molecules and fluids this would be called __________________ PINOCYTOSIS LABEL THE TONICITY DIAGRAMS HYPERTONIC ISOTONIC HYPOTONIC Name the two types of transport proteins that carry out facilitated diffusion Channels and carriers Which of these would increase membrane fluidity? A a) greater proportion of unsaturated phospholipids b) greater proportion of saturated phospholipids c) a lower temperature d) relatively high protein content in membrane e) greater proportion of relatively large glycolipids compared to lipids having smaller molecular masses Campbell Ch 7 self quiz Diffusion is a spontaneous process in which down their concentration molecules move _______ gradient. up down Channel proteins Aquaporins are ______________________ for water molecules a) carrier proteins b) channel proteins c) active cotransporters d) ligands e) a kind of Na+-K+ pump Name the genetic disorder you learned about in BIO I in which there is a mutation in the protein that transports Cl- ions causing a build up of thick mucous in the lungs and digestive organs Image from: BIOLOGY by Miller and Levine; Prentice Hall Publishing ©2006 Cystic fibrosis Explain why plant cells don’t undergo cytolysis in hypotonic environments but animal cells do. Cell wall is rigid and keeps them from bursting Give an example of an amphipathic molecule. Phospholipids have both hydrophobic and hydrophilic regions; so do many proteins Method of splitting a cell membrane along the middle of the phospholipid bilayer Freeze fracture Name this cell model which proposed that membranes were “sandwiches” of phospholipids between two layers of hydrophilic proteins Davson-Danielli model A mole of table sugar and a mole of vitamin C are equal in their A. mass in daltons C. A mole of anything B. mass in grams C. number of molecules contains 6.02 X 10 -12 molecules. D. number of atoms E. volume Campbell Self quiz A _______________ amphipathic molecule has both a hydrophilic region and a hydrophobic region. Selectively or semi- permeable Cell membranes are called _____________________ because they allow certain substances to pass through, but keep others out. The proton pump is the main electrogenic pump plant in ________________ cells. plant animal Membrane proteins that span the membrane touching both sides are called transmembrane proteins _________________ Name the molecules which can be attached to proteins or lipids in cell membranes and are involved in cell recognition carbohydrates How would you make a 0.5 M solution of sodium chloride? Campbell Concept Check 3.2 (The atomic mass of Na is 23 daltons and Cl is 35.5 daltons) 58.5 daltons Mass of 1 mole of NaCl = _______ (23 + 35.5) 58.5 g NaCl X 0.5 moles = _______ 29.25 g/L 1 mole 1L Dissolve 29.25 g of NaCl in water to make 1 L of solution How many grams of acetic acid (C2H4O2) would you use to make 10 L of a 0.1 M aqueous solution of acetic acid? (The atomic masses in daltons are approximately 12 for C, 16 for O, and 1 Campbell Chapter Self Quiz for H) A. 10.0 g B. 0.1 g C. 6.0 g D. 60.0 g E. 0.6 g Mass of 1 mole of acetic acid C = 2 X 12 = 24 daltons H = 4 X 1 = 4 daltons O = 2 X 16 = 32 daltons 60 daltons 60.0 g X 0.1 moles = 6 g/L X 10 L = 60.0 g 1 mole 1L 60.0 g of acetic acid and add water to make 10 L of solution (D is correct) Which type of transport maintains the membrane potential in animal cells? Sodium-potassium pump Molecules that bind specifically to a receptor site ligands on another molecule are called ____________ Name the transport process you learned about in which ligands are involved in the uptake of molecules Receptor mediated endocytosis Electrogenic pumps create voltage across cell membranes making the cytoplasm more negative _______________ than the extracellular fluid. negative positive The water potential ψ for pure water at zero atmospheric pressure is ______ decreases the water The addition of solutes ___________ potential. increases decreases USE lower and higher to complete the following: higher Water always moves from an area with ________ lower water water potential to an area with _______ potential. Water with solutes dissolved in it will have a lower ______________ water potential than plain water. According to the fluid mosaic model of membrane structure, proteins of the membrane are mostly A. spread in a continuous layer over the inner and outer surfaces of the membrane B. confined to the hydrophobic core of the membrane C C. embedded in a lipid bilayer D. randomly oriented in the membrane with not fixed inside-outside polarity E. free to depart from the fluid membrane and dissolve in the surrounding solution Campbell Concept check An artificial cell consisting of an aqueous solution enclosed in a selectively permeable membrane has just been immersed in a beaker containing a different solution. The membrane is permeable to water and to the simple sugars glucose and fructose, but completely impermeable to the disachride sucrose. Which solute(s) will exhibit a net diffusion into the cell? fructose Which solute(s) will exhibit a net diffusion out of the cell? glucose An artificial cell consisting of an aqueous solution enclosed in a selectively permeable membrane has just been immersed in a beaker containing a different solution. The membrane is permeable to water and to the simple sugars glucose and fructose, but completely impermeable to the disachride sucrose. Campbell Concept check Which solution-the cell or the environment- is hypertonic to the other? Cell (0.05 M) is hypertonic to the environment (0.03 M) In which direction will there be a net osmotic movement of water? Water will move into the cell After the cell is placed in the beaker, which of the following changes will occur? A. The artificial cell will become more flaccid. B. The artificial cell will become more turgid. C. Some water molecules will flow out of the cell, but the majority will flow in. D. The membrane potential will decrease E. In spite of the inability of sucrose to cross the cell membrane, eventually the two solutions will become isotonic. B, C, E are correct Campbell Concept check