MITOCHONDRIAL GENETICS Origin of Mitochondria Endosymbiont Theory • Similar size to certain free-living bacteria • Similar chromosome & cytoplasm to bacteria • Similar ribosomes to bacteria • Similar cell division to bacteria • Independent replication & gene expression Mitochondrial Chromosomes 2-10 @ per mito 1000-10000 per cell Nuclear (Cellular) Chromosomes 3.2 billion total nucleotide pairs 0.32-1.6 billion total nucleotide pairs Genetically Controlled Processes in Mitochondria • 13 genes for respiratory chain proteins • Subunits of the ATPase complex • Subunits for the NADH-dehydrogenase complex • 22 genes for tRNAs • 2 genes for rRNAs • Maintenance of cellular energy levels Ataxia v. Ataxia Human Mitochondria • 2-10 chromos per mito • 13 protein-encoding regions • 3 subunits of cytochrome c oxidase complex • Cytochrome b complex • 2 subunits of the ATPase complex • 60% occupied by six subunits of NADH dehydrogenase complex • 22 tRNAs • 2 rRNAs • other Stability of Mitochondrial DNA • Mutatation rate 10X higher than nuclear DNA • Mutations generated by oxidation by-products • No protective histones • Ineffective DNA repair • Typically point mutations or deletions • Tolerance for deletions variable • Some deletions recurring in unrelated patients Mitochondrial Diseases General Characteristics • • • • • Penetrance usually complete (100%) Expressivity highly variable Age of onset variable Frequently limited to specific tissues Usually appear as reduced muscle strength together with degeneration of other tissues • Affects organs with high energy requirements: brain, heart, skeletal muscle, eye, ear, liver, pancreas, and kidney Some Mitochondrial Diseases • Kearns-Sayre syndrome opthalmoplegia, retinal degeneration, cardiomyopathy • Lactic-Acidosis-Encephalomyopathy-Stroke syndrome • Neurogenic muscle weakness with ataxia and retinitis pigmentosa syndrome • Pearson syndrome bone marrow and pancreas failure • Diabetes-deafness syndrome Maternal Transmittance of Mitochondrial Disease heteroplasmy Mitochondrial Diseases General Characteristics • • • • • Penetrance usually complete (100%) Expressivity highly variable Age of onset variable Frequently limited to specific tissues Usually appear as reduced muscle strength together with degeneration of other tissues • Affects organs with high energy requirements: brain, heart, skeletal muscle, eye, ear, liver, pancreas, and kidney At the course download site: mitogenetics.ppt