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The preparation of CaCO3 nanoparticles and
application in drug microcapsules
Bai Zhijun
2014.4.8
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I. Introduction
II. Experiment
III. Significance
IV.Technology
2
I. Introduction
1. Unlike capsules
Nanometer level
2. Why drugs need to wrap？
3. Nowadays？
Hydrophilic or hydrophobic drug;
Targeting;
Toxicity and side effect;
……
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Nowadays
微球
Microspheres
微囊
Microcapsules
Drug Microcapsules Preparation
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II. Experiment
EDTA
亲水药物
CaCO3纳米颗粒
PSS
PAH
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1）Preparation of
CaCO3
CaCl2+drug+Na2CO3
CaCO3- drug
nanoparticles-drug
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2）Polyelectrolyte modification
Layer By Layer
n
NH2
HCl
n
SO3Na
聚乙烯苯磺酸钠
聚烯丙基胺盐酸盐
PSS
PAH
10
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3）Washing treatment
washing
SEM (scanning electron microscopy)
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CLSM (Confocal laser scanning microscopy)
CdTe QDs ： Green（inside）and Red（outside）
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CLSM (Confocal laser scanning microscopy)
CdTe QDs ： Green（inside）and Red（outside）
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III. Significance
1.It can reduce drug side effects；
2.This is a universal package technology ；
3.This is a new drug formulations；
4. This is a major breakthrough for the development
of pharmaceutical formulations；
5. This breakthrough has important social value and
economic benefits;
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IV.Technique
1) Coprecipitation
2) Layer by layer self-assembly technique
3) Centrifugal separation technique
4) Microscopic imaging technique
5) Using EDC connecting amino with carboxyl
technique
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共沉淀：指的是一种沉淀从溶液中析出时
，引起某些可溶性物质一起沉淀的现象。
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层层自组装(layer-by-layer self-assembly，LBL)
是 利 用 带相反电荷的 聚 电 解 质 交替沉积在基板（
substrate） 表 面 来 制备聚电解质自组装多层膜
(polyelectrolyte self-assembled mulilayers)的方法，是
一种简易、多功能的表面修饰方法。
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离心分离技术
G＝1.11×10
(-5)
×R×(rpm)2
G为离心力，一般以ｇ(重力加速度)的倍
数来表示。
(rpm)2即：转速的平方。
R为半径，单位为cm。
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普通光学显微镜成像原理
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EDC,1-ethyl(3-dimethylaminopropyl)carbodiimide hydrochloride
1-乙基-（3-二甲基氨基丙基）碳二亚胺盐酸盐
反应机理
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水相CdTe Qds的合成简介
NaBH4 还原Te粉到Te2-，与CdCl2形成
CdTe量子点，随着回流加热时间的不同，
颗粒聚集的大小不同，发光不同。用巯基
乙酸做稳定剂和保护剂，所以水相合成的
量子点表面带有羧基。
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APTES modification
NH2
O
HCl
n
PAH
Si
OHC
CHO
O
O
Glutaraldehyde
APTES
PAH- NH2 + OHC（CH2）3CHO + NH2-APTES
APTES-N=C—C=N-PAH
NH
2
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APTES cross-link
O
H
水解
O
O
H
O
H 2N
Si
缩聚
S
i
H
N
2
O
O
H
H2N
NH2
HO
Si
Si
OH
O
O
HO
O
OH
O
O
H2N
Si
O
Si
NH2
O
O
O
HO
O
Si
HO
OH
Si
OH
NH2
NH2
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Drug sustained-release
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Drug targeting release
Since most anticancer drugs are unable to
differentiate between diseased and healthy
cells, systemic toxicity and undesired side
effects can result.
Passive targeting（EPR）
Active targeting（SMN）
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EPR(enhanced permeability and retention ) effect
Smart
Multifunctional
Nanostructure
Aptamer
SMN
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Aptamer (sgc8) specifically binds to cell membrane
receptor protein tyrosine kinase 7 (PTK7).
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