June 12th-14th, 2013
Collaborative E-Learning
application for
electroporation-based
biomedical applications
Selma Corovic
Background and motivation
- Electroporation of cells and tissues – human, animal or plant
cells for different biomedical and biotechnological applications
clinical electrochemotherapy - cancer treatment
- Electroporation based biomedical applications require
multidisciplinary expertise: oncologists, physicists, engineers…
Collaborative E-Learning application
Oncology
Biomedical engineering
E-Learning application
Collecting
the knowledge
Designing
the content
Research
E-learning application on
cells and tissues
+
Clinical trials
Improving the content
based on results
E-Learning enviroment
(EBTT)
Evaluation (pedagogical efficiency and usability)
E-Learning enviroment
Electroporation based technologies and treatments
International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE
November 18-24, 2012, Ljubljana, Slovenia
Lectures
Lectures were recorded
Practical work
Electroporation
molecules
cell membrane
Important parameter for effective electroporation
local electric field distribution E [V/cm]!
Local electric field distribution E >Erev
Electroporated
Cell membrane
Cell
Tissue
Applications of Electroporation
Electroporation is used in:
biomedical
- clinical electrochemotherapy, non-viral gene electrotransfer for gene therapy
and DNA vaccination, transdermal drug delivery…
- liquid food sterilization, water treatment, electrofusion of cells…
UTI = −1.5 r E cos (φ)
(a) The electroporation of cell membrane first occurs within
the cell area facing the electrodes
(b) Different electroporation regimes:
reversible Erev < E < Eirrev and irreversible E >Eirrev
Electrochemotherapy =
= chemotherapy + electroporation
Electric pulse
generator
Chemotherapeutic
surrounds the cells
Increased membrane Pores reseal
permeability allows
access to the cytosol
Electrodes
time
Tumour
Electric pulse application
Injecting chemotherapeutic
Protocols and local electric field distribution in tumors
Protocols used in clinical electrochemotherapy today include:
8 pulses of 100 µs duration, f=1 Hz or f=5 KHz, U/d =1300 V/cm
drug
injection
+
Result
Local electric field distribution:
U/d
Importance of an adequate local electric field for
successful tissue electroporation (example: unsuccessful tumor treatment)
Clinical use of electrochemotherapy
Number of
patients
No. of clinical
centres
2006
130
SOP
2007
300
37
2008
600
53
2009
800
55
2010
1000
83
2011
1500
92
2012
3000
108
Reimbursed in:
Germany, UK, Italy, Portugal, Denmark, Greece, Slovenia.
Poland – in progress for reimbursement. ECT approved by Minister of
Health.
Clinical use of electrochemotherapy
E-Learning application
Collecting
the knowledge
Designing
the content
Research
E-learning application on
cells and tissues
+
Clinical trials
Improving the content
based on results
E-Learning enviroment
(EBTT)
Evaluation (pedagogical efficiency and usability)
Laboratory of bio cybernetics
http://lbk.fe.uni-lj.si
Lea Retelj, Ajda Bicek, Matevz Leskovsek, Jasna Krmelj, Tadej Kotnik, Dusa Hodzic, Marko Usaj, Gorazd
Pucihar, Alenka Macek Lebar, Samo Mahnic, Barbara Mali, Tomaz Jarm, Jaka Cemazar, Selma Corovic,
Denis Pavliha, Bor Kos, Masa Kanduser, Natasa Pavselj, Janez Zigon, Ivan Pavlovic, Stanislav Rebersek,
David Slavic, Melvin Essone, Jure Jelenc, Matej Rebersek, Tina Batista Napotnik, Mitja Gliha, Sasa Haberl,
Peter Kramar, Igor Marjanovic, Barbara Zorec, Matej Kranjc, Damijan Miklavcic.
(Missing from the photo: Karel Flisar, Marija Marcan, Andraz Polak)