Chapter 6 A Tour of the Cell Much of our knowledge of cells has come about with the assistance of microscopes. Many scientists studied various aspects of living things. With this knowledge, we have come to accept three basic ideas regarding living things… These ideas are known as The Cell Theory. The Cell Theory is one of the most important theories in biology. All living things are composed of cells Cells are the smallest unit of structure and function in living things. Cells can only come from pre-existing cells All cells are classified into one of the following categories Eukaryotic (complex) Prokaryotic (simple) Nucleus No nucleus Organelles (many) No organelles 10-1000 micrometers in size 1-10 micrometers in size Fossil evidence dates back to 3.5 bya Unicellular Bacteria Fossil evidence dates back to 1.5 bya Unicellular or multicellular Plants, Animals, Protists, and Fungi Highly compartmentalizedcell is separated into “rooms” by membranes “walls” Prokaryotic vs. Eukaryotic Notice that the prokaryotic cell has a “nucleoid region” and a cell wall. If you were looking at a picture of a cell, what would you look for to determine if the cell is a prokaryote of a eukaryote? Nucleus? Organelles? Size? All cells have a cell membrane & ribosomes. Basic Structures and Organelles in Eukaryotic Cells Why is each cell structure crucial to the function of the cell and to the whole organism? Cell (“Plasma”) Membrane Structure: Phospholipid Bilayer studded with proteins and other molecules Function: Gate Keeper – regulates what enters and exits the cell and maintains homeostasis Location: Surrounds cell Cell Wall Structure: Made of cellulose chitin ( fungi) or Function: Structure/Support/Protection Location: Outside of the cell membrane (only in plants, algae, bacteria and fungi) Nucleus Structure: Large and membrane-bound Function: Contains DNA (chromatin) Control center of cell- directs the activity of the cell Location: Animal – center Plant – near the cell membrane Nuclear Membrane “Envelope” Structure: Double membrane with many pores Function: Keeps DNA (chromatin) contained and allows substances to enter/exit the nucleus Location: Surrounds nucleus Nucleolus Structure: Dark structure in the nucleus/made of RNA and protein Function: Produces ribosomes Location: Inside the nucleus Cytoplasm Structure: Cytosol (fluid) and organelles Function: Allows movement of substances throughout the cell Location: The area outside nucleus and inside cell membrane Ribosomes Structure: Made of RNA, small, not membrane bound Functions: Manufactures proteins Location: Free floating in cytoplasm, or attached to Endoplasmic Reticulum Found in both Prokaryotic & Eukaryotic cells Chloroplast Structure: Double outer membrane with inner membrane in the form of disks and tubules Contains chlorophyll Function: Converts solar energy into chemical energy (carbs) by photosynthesis. Location: In cytoplasm (found in plants, + algae) Structure: Bean-shaped organelle Mitochondria with a highly folded inner membrane. Function: Cellular Respiration Converts energy in food (carbs) to a form of energy the cell can use (ATP) “Powerhouse” of cell Location: Cytoplasm (all Eukaryotic cells) Endoplasmic Reticulum (ER) Structure: System of membranes extending throughout cytoplasm. Passageways/tunnels through cell Function: Smooth ER – w/o ribosomes (builds lipid molecules) Rough ER – w/ribosomes attached (proteins for export) Location: Cytoplasm - from nucleus to cell membrane Golgi Apparatus Structure: Membranes in flattened sacs Function: Recieves proteins from ER, modifies, stores, or packages (proteins) and other substances for export Location: Cytoplasm Vacuoles Structure: Membrane-bound sacs Plants – 1 large (water) Animals – many small Function: Storage Location: Cytoplasm Structure: Small membrane bound sacs filled with digestive enzymes Mainly found in Animal cells/very few plant cells have them Function: Digests old/worn out structures, organelles or molecules or engulfed organisms Location: Cytoplasm Lysosome Cytoskeleton Microtubules Structure-straight hollow tubes of protein Function–give rigidity and shape to cell, tracks for organelles to move along Microfilaments Structure-thin solid rods of protein (internal skeleton) Function-allow cell to move or change shape Location: Cytoplasm Cell Analogy Activity ANALOGY: similarity between like features of two things, on which a comparison may be based. Example: the heart is similar to a (water) pump ? Flagella StructureLong, thin, whip-like tail (microtubules) FunctionMovement Cilia Structureshort hair-like (many) (microtubules) Functionmove fluid over surface or movement Plastids Double layered membranous sacs (Plants only) Storage Leukoplasts – starch, lipids or proteins Chromoplasts produce/store pigments Chloroplasts – chlorophyll/photosynthesis Adensosine Triphosphate the most important energy-storing molecule in living things! ATP transports energy within cells. Energy is stored in the bonds between the phosphate groups. In order to release energy from ATP, the terminal (last) phosphate group must be removed/ This can be accomplished through hydrolysis. Hydrolysis of ATP (energy is released and made available for the cell.) When cells need energy, a phosphate group is removed from ATP and it is converted to ADP and energy is released. RELEASE ENERGY STORE ENERGY If cells want to store energy, ADP can be converted to ATP by adding a phosphate group back on. Formation of ATP – cells use the ADP to store energy. They add the phosphate group back on to ADP converting it to ATP. phosphate removed 36 ATP – glucose 80 ATP – Triglyceride Cells use 1 million ATP /sec Plant Cells •Cell Membrane •Nucleus •Cell Wall •Nuclear Membrane •Chloroplast •Nucleolus •1 Large Vacuole •Cytoplasm (water) •Ribosomes •Plastids •Mitochondria •Stores energy as •Endoplasmic Reticulum starch •Golgi Apparatus •Cytoskeleton Animal Cells •Many Small vacuoles •Lysosomes •Stores energy as glycogen Plant vs. Animal Cells (Eukaryotic cells) Levels of organization in multicellular organisms Cells Cells are the basic unit of structure and function in living things. The cells of multicellular organisms are specialized and perform many different functions. Animal-neuron, muscle, RBC, epithelial, bone, etc… Plant-guard, epidermal, etc… Different kinds of plant cells Onion Epidermal Cells root hair Root Hair Cell Guard Cells Different kinds of animal cells white blood cell Amoeba red blood cell muscle cell cheek cells sperm nerve cell Paramecium Unicellular Protists Tissue A group of similar cells that perform a particular function Animals: epithelial, muscular, connective, nervous Plants: epidermal, vascular, mesophyll Organ Different tissues group together to carry out specialized functions Heart: consists of muscles, nervous tissue and blood vessels (kidney, liver, pancreas) Leaf: consists of epidermis, mesophyll and vascular tissue (roots, stems, flowers) System Several organs and tissues work together to carry out a particular set of functions in a coordinated way Human: digestive, respiratory, excretory, circulatory reproductive systems, etc… Plant: root and shoot systems The Respiratory System Organism The highest level of organization in any living thing! Example: you Levels of Organization CELLS (muscle cells,nerve cells) TISSUES (muscle, epithelium) ORGANS (heart, lungs, stomach) SYSTEMS (circulatory system) ORGANISM (human) Plant Cells vs. Animal Cells Plant Cells Animal Cells Cell wall No cell wall Large central vacuole Small vacuoles or none Chloroplasts (plastids) No chloroplasts or plastids No lysosomes Lysosomes Store energy as Store energy as starch glycogen Both: Cell membrane, Nucleus, Nucleolus, Nuclear membrane, Cytoplasm, Ribosomes, Mitochodria, Endoplasmic Reticulum, Golgi Apparatus, Cytoskeleton