UNIT 2: THE CELL Chapter 7 (Cell Structure and Function) CHAPTER 7.1: LIFE IS CELLULAR Key questions What is the cell theory How do microscopes work How are prokaryotic and eukaryotic cells different Vocab Cell Cell theory Cell membrane Nucleus Eukaryote prokaryote DISCOVERY OF THE CELL Originally “seeing is believing” Early microscopes 1665, Robert Hooke: made a compound microscope to see a slice of cork Several small compartments = “Cells” Anton van Leeuwenhoek: used a lense to look at pond water and material within his mouth 1838: Matthias Schleiden discovered allplants are made of cells Theodore Schwann discovered all animals are made up of cells 1855: Rudolf Virchow concluded that new cells can be created by the division of cells THE CELL THEORY The cell theory states: All living things are made up of cells Cells are the basic units of structure and function in living things New cells are produced from existing cells EXPLORING THE CELL How do microscopes work? use lenses to magnify the image with light or electrons Light microscopes: Uses reflected light: Only good for 1000x magnification Can see items as small as 1 millionth or a meter Must have stains or dyes because of transparency of item Electron microscopes: 1 billionth of a meter Transmission EM Beams of electrons pass through the thin samples only 2-D Scanning EM Scans the surface of the sample Giving a 3-D of specimen surface PROKARYOTES AND EUKARYOTES Cells come in varying shapes and sizes Typical size being 5-50 micrometers Smallest being .2 micrometers and the largest being 1 millimeter in diameter (chaos choas; the giant amoeba) All cells have a cell membrane (plasma membrane) CHAOS CHAOS Two categories: Organized nucleus Eukaryote No organized nucleus Prokaryote Nucleus: a large membrane-enclosed structure that contains genetic material like DNA and controls many of the cells activities EUKARYOTE PROKARYOTE 7.1 ASSESSMENT REVIEW 1. Cell 1. 2. 3. 2. Microscope 1. 2. 3. What is a cell? What 3 statements make up the cell theory How did the invention of the microscope help the development of the cell theory How do microscopes work? What does it mean if a micrograph is “false-colored” Features of a cell 1. 2. What features do all cells have What are the differences between prokaryotes and eukaryotes? 7.2 CELL STRUCTURE Key questions: What is the role of the Nucleus What are the functions of the Vacuoles, lysosome, and the cytoskeleton What organelles help make and transport proteins What are the functions of chloroplasts and mitochondria? What is the function of the cell membrane? Vocab: Cytoplasm Organelle Vacuole Lysosome Cytoskeleton Centriole Ribosome Endoplasmic reticulum Golgi aparatus Chloroplast Mitochondrion Cell wall Lipid bilayer Selectively permeable CELL ORGANIZATION http://www.youtube.com/watch?v=D3fOXt4MrOM http://www.youtube.com/watch?v=LMVQ-INMSVw Simple breakdown into 2 groups: 1. Nucleus 2. Contains nearly all cell’s DNA and the coded instructions for making proteins and other important molecules Cytoplasm The fluid portion of the cell outside the nucleus **Both Eukaryote and prokaryote cells have cytoplasm** ANIMAL CELL PLANT CELL NUCLEUS “The control center” Nuclear envelope Dotted with holes Allows for particles (messages) to flow Chromosomes Carry genetic information Chromatin Nucleolus: Assembly of RNA ORGANELLES Specialized structures that act like organs within the cell aid with storage, clean up, and support Vacuoles (storage) storage of materials like water, salts, proteins, and carbohydrates In plant cells, one large one filled with liquid to help with rigidity with things like flowers and leaves Vesicles: Store and move material between organelles and to and from the surface of the cell Lysosome: (cleaning crew) get rid of the “Junk” Small organelles filled with enzymes Break down lipids, carbohydrates, and proteins into smaller molecules that can be used throughout the cell Breaking down useless organelles Link to disease when not functioning ORGANELLES…CONTINUED Cytoskeleton: (the building) Shape and internal organization of the cell Aids in movement of cell 2 principle protein filaments Microfilaments: actin Microtubules: hollow structures of protein GREEN = MICROTUBULES RED = ACTIN MAKING PROTEIN 1. Proteins are responsible for so many essential functions for life…therefore it needs to be produced in high amounts Ribosomes: 2. small particles of RNA and protein found throughout the cell Assemble protein http://www.youtube.com/watch?v=D3fOXt4MrOM Endoplasmic Reticulum: 3. Eukaryotic Cells Formation of lipid component of cell membrane Protein packaging 2 types of ER Rough ER: ribosomes Smooth ER: no ribosomes Golgi aparatus: packaging of proteins: 1. Modifies, sorts, and packages proteins and other materials from the ER for storage or release from the cell. THE PROCESS FROM NUCLEUS TO CELL MEMBRANE ENERGY FACTORY All things need energy…how do cells get theirs? Chloroplasts: these organelles capture the energy from sunlight and convert it into food that contains chemical energy in a process called photosynthesis. Cholorphyll ENERGY CONTINUED… Mitochondria: this organelle converts chemical energy stored in food into compounds that are more convenient for the cell to use. Two layer membrane (like the chloroplasts) **2 interesting facts** Heredity linked to mother Carries own DNA…may have been Independent micro-organism at one time? BOUNDARIES OF CELL Cell wall: A strong protective/supportive layer around the cell support shape protect the cell NOT IN ANIMAL CELLS!! Cell membranes: regulate what enters and leaves a cell and also protects and supports the cell. Lipid bilayer: gives cell membrane a flexible structure that forms a strong barrier between the cell and its surroundings LIPID BILAYER The lipid has two parts One hydrophobic One hydrophilic These layers create a water Tight envelope around the cell! *protein molecules aid in allowing particle to pass through the membrane making it selectively permeable FLUID MOSAIC 7.2 REVIEW 1. 2. 3. 4. 5. What are the major parts of the cell? What is the function of lysosomes? What is the difference between smooth and rough ER? What is the function of mitochondria? Why is the cell membrane sometimes reffered to as a fluid mosaic? 7.3 CELL TRANSPORT Key Questions: What is active transport What is passive transport Vocab: Diffusion Facilitated diffusion Aquaporin Hypertonic Hypotonic Osmotic pressure CELL TRANSPORT…PASSIVE TRANSPORT Read “think about it” on pg. 208 What is Passive transport: the movement of materials across the cell membrane without using cellular energy. Diffusion: The process by which particles move from an area of high concentration to a an area of lower concentration. Cellular cytoplasm is made of many different substances dissolved in water. In any solution particles move constantly They collide and spread out randomly **diffusion is the driving force behind the movement of many substances across the cell membrane** PASSIVE TRANSPORT Facilitated Diffusion: The process in which particles that cannot directly diffuse across the membrane pass through special protein channels These particles pass through protein channels Channels are specifically sensitive to certain particles It is the “speed pass” OSMOSIS Osmosis(example of facilitated diffusion): Diffusion of water through a selectively permeable membrane -Because of the lipid bilayer, water needs a way in…so Aquporins are specific protein channels to allow for H2O to pass quickly through the cell membrane. In the presence of a hypertonic (++) and hypotonic(--) solution, equilibrium is needed= a isotonic solution Hypertonic: “above strength” Hypotonic: “below strength” Isotonic: “same strength” Osmotic pressure:? OSMOSIS IN ACTION ACTIVE TRANSPORT The movement of materials against a concentration difference which requires energy. The active transport of small molecules or ions across the cell membrane are often carried out by Transport proteins – protein pumps The active transport of large molecules and larger materials are transported by process called endocytosis / exocytosis. MOLECULAR TRANSPORT Most cells transport: Calcium Potassium Sodium ions Energy (ATP) is used to power the “pump” BULK TRANSPORT Endocytosis: Taking in of material Phagocytosis: extensions reach out to the particle and package it within a vesicle Exocytosis: Vesicle bonds with membrane and releases material outside the cell WHICH IS WHICH? CHAPTER 7.3 REVIEW 1. what happens in diffusion? 2. describe osmosis Difference between diffusion and facilitated diffusion How is active and passive transport different 2 major types of active tranport endocytosis vs. exocytosis 7.4 HOMEOSTASIS AND CELLS Key Questions: How do individual cells maintain homeostasis How do cells of multicellular organisms work together to maintain homeostasis? Vocab: Homeostasis Tissue Organ Organ system receptor HOMEOSTASIS Homeostasis: responding to the environment through growth, transforming energy, and reproduction * both in unicellular and multi-cellular organisms: MULTICELLULAR HOMEOSTASIS Cells of multicellular organisms become specialized for particular task and communicate within one another to maintain homeostasis Tissue: groups of cells to perform a function Organ: groups of tissues working together to perform a function Organ system: a group of organs working together to perform a function Communication: receptors: on or in a cell, a specific protein protein to whose shape fits that of a specific molecular messenger. REVIEW 7.4 What is homeostasis? Single cell organisms vs. multicellular Cellular specialization Receptors?