6 Kingdoms of Life Part 1: Archaebacteria, Eubacteria, Protist, and Fungi 1 Characteristics of Living Things • 5 characteristics of living things – Made up of cells – Reproduce – Based on genetic code – Metabolism – Homeostasis • To be considered living, an object MUST have ALL 5 characteristics 2 • As living things are constantly being investigated, new attributes are revealed that affect how organisms are placed in a standard classification system. 3 Classification • Scientists have determined seven levels of classification: – Kingdom = Kings – Phylum = Pass – Class = Classes – Order = to Order – Family = Families – Genus = and their Good – Species = Sons around 4 Classification of Modern Humans • • • • • • • Kingdom = Animalia Phylum = Chordata Class = Mammalia Order = Primates Family = Hominidae Genus = Homo Species = sapiens 5 • The grouping of organisms into KINGDOMS is based on 3 factors: – 1. Cell Type – 2. Cell Number – 3. Feeding Type 6 1st Criterion for Kingdom Divisions: Cell Type Prokaryotes or Eukaryotes 7 6 kingdoms 1. Eubacteria 2. Archaebacteria 3. 4. 5. 6. Protista Fungi Plantae Animalia Prokaryotes Eukaryotes 8 2nd Criterion for Kingdom Divisions: Cell Number •Unicellular- single celled organism – protozoans, bacteria, some algae •Multicellular- many celled organism – cells start to specialize/differentiate 9 • Unicellular • Multicellular 10 3rd Criteria for Kingdom Divisions: Feeding Type –Autotroph or Producer Make their own energy source –Heterotroph or Consumer Must eat other organisms to survive Includes decomposers – those that eat dead matter! 11 Archaebacteria and Eubacteria Kingdoms 12 Bacteria Microbiology- the study of very small, microscopic organisms – – – – – Bacteria Fungi Viruses Protists Etc. 13 Bacteria=Prokaryotes Prokaryotes No organelles except ribosomes NO NUCLEUS Eukaryotes Lots of organelles INCLUDING NUCLEUS 14 Prokaryotic History • Oldest organisms: 3.5 billion yrs. old. • Live in almost every environment. 15 Eubacteria • Kingdom Eubacteria – Common environments – Believed to be the ancestors of mitochondria and chloroplasts--organelles in eukaryotic cells 16 Archaebacteria • Kingdom Archaebacteria – Found in extreme environments-extremophiles – Ancient bacteria-gave rise to eukaryotic cells 17 Characteristics-Size • Size • Red blood cell is 250X’s larger than a bacterium • 1 gram of soil can contain 2.5 BILLION bacteria • Relative bacteria size 18 Characteristics-Shapes • Shapes: – Cocci- round – Bacilli- rod-shaped – Spirilla- spiral 19 Prokaryotic Structure • Interior structures – Has DNA and cytoplasm— no nucleus or other membrane bound organelles EXCEPT ribosomes – Ribosomes- the protein making factories of all cells 20 Prokaryotic Structure • Exterior structures – Flagella-whip-like tail for locomotion – Cell membrane to control what goes in and out – Cell wall for protection 21 2 Types of Cell Walls • 2 types of cell walls found in bacteria – Identified as Gram + or Gram – – There’s a chemical difference b/t them. 22 Gram staining • Special staining process • “Gram positive is purple; Gram negative is not.” 23 2 Types of Cell Walls • Gram + – Thick cell wall – Holds purple stain, so cells look purple • Gram —Two thin layers make up cell wall —Doesn’t hold purple stain so appears pink 24 2 Types of Cell Walls • Treatment of illness due to these bacteria is different! – Gram - : bacteria that stain pink and are generally NOT affected by antibiotics • i.e. E. coli – Gram +: bacteria that stain purple do to a thick cell wall and are affected by antibiotics • i.e. S. pneumoniae 25 Prokaryotic Reproduction • Binary fission- process of asexual reproduction where 1 becomes 2. – Results in clones – Colony- 1000’s of bacteria that result from one undergoing binary fission 26 How are prokaryotes so diverse? • They have several ways of exchanging genetic info • Conjugation--exchanging DNA through a straw-like tube called a pilus • Transformation—another method of transferring genes between bacteria. 27 Useful Prokaryotes • Decomposers- Recycle nutrients such as CO2 , water, nitrogen, and phosphorus 28 Useful Prokaryotes •Nitrogen fixation- soil bacteria take nitrogen gas from the air (N2) and change it into a useable form that plants can absorb (NH3- ammonia.) •Plants use the nitrogen to produce their proteins and DNA. •Some bacteria are photosynthetic and also provide oxygen N2 YUMMY! Bacteria NH3 29 Useful Prokaryotes • • • • Food-- yogurt, olives, pickles, chocolate Drugs -- insulin production Clean up oil spills Animal digestion and vitamins, including our own • Microbe Discovery Movie 30 • • • • Harmful Prokaryotes Pathogen- disease causing organisms. Pathologists -scientists who study pathogens. Not many bacteria are pathogenic— ONLY 1%! Disease Transmission: a.) Water b.) Air c.) Food d.) Animals/Insects e.) Human Contact 31 Prokaryotic Diseases • • • • • • Tuberculosis Syphilis Bubonic Plague Typhus Tetanus Lyme Disease 32 Controlling Prokaryotic Growth • What do bacteria require to live and reproduce? - Food, water, and the right climate. -Give bacteria these things, and they grow; remove them and they don’t. 33 Nutrition and Energy • How do bacteria “eat”? – 1.) Autotrophic- “self-feeders” • Photosynthetic- MAKE energy source and release oxygen • Can also be chemosynthetic – 2.) Heterotrophic- “other-feeders” • GET energy source outside themselves – Consumers – Decomposers – Parasites 34 Feeding Prokaryotes in the Lab • Plastic Petri dishes have a Jell-O like substance called AGAR with nutrients and water for bacteria to grow on. 35 GROWTH CURVE 36 Controlling Prokaryotic Growth • Antibiotics • Sanitizing--Antiseptics and Disinfectants • Freezing • Cooking • Pasteurizing • Dehydrating • Vaccination 37 Antibiotics • Alexander Fleming • Mold on his Petri dish had a zone of inhibitionarea in which bacteria didn’t grow. • Mold released the antibiotic penicillin BACTERIA • Antibiotic=against life; any substance produced by a microbe that slows the MOLD growth of other microorganisms. 38 Antibiotics • Antibiotics are made by : – Fungus (mold) – Other bacteria, the most common Streptomyces. – Present day antibiotics are synthetic modifications of naturally occurring ones. • Work well on Gram + bacteria • NOT the same thing as aspirin or tylenol, which are pain killers BACTERIA Each paper disk has antibiotics 39 on it. Which antibiotic is more powerful? Antibiotic Resistance • Antibiotic resistance- some bacteria are not affected by certain antibiotics! • Can be resistant due to: – Special cell walls (i.e. Gram – bacteria) OR – Special antibiotic resistant genes • Don’t finish antibiotics: – Weaker bacteria destroyed. – **Resistant bacteria still live and pass on resistant genes through binary fission, conjugation and transformation – Conjugation animation Movie 40 Sanitizing • Antiseptics- chemicals used to inhibit growth of bacteria on living tissues • Disinfectants- chemicals used to inhibit growth of bacteria on NON-living things. 41 Freezing • How would this control the growth of bacteria? • Would freezing kill all the bacteria? 42 Cooking • Cooking can control bacterial growth and kill most bacteria if heated to certain temps—165F or hotter. • Use a meat thermometer • Wash hands after handling raw meat 43 Pasteurizing • Pasteurization- using heat to kill bacteria in liquids. 44 Dehydrating • Dehydration- removal of water from a substance • How would this control the growth of bacteria? 45 Vaccination • Vaccination- a shot, pill, or mist that prevents you from getting a disease. DOES NOT CURE YOU. • Fast and strong immune system memory cells produced which provides immunity just like if you got the disease (i.e. tetanus.) 46 Vaccination • Can use weakened (attenuated) bacteria or viruses • MOSTLY use parts of bacteria or viruses—acellular • Vaccine video 47 BACTERIA KINGDOMS PROKARYOTIC UNICELLULAR HETEROTROPHS AND AUTOTROPHS ARCHAEBACTERIA OLDEST FORM OF LIFE LIVES IN EXTREME ENVIRONMENTS EUBACTERIA 3.5 BILLION YEAR OLD ANCESTORS MORE RECENTLY EVOLVED LIVES IN MORE COMMON ENVIRONMENTS 48 Protist Kingdom 49 Kingdom Protista 50 Protists, what are they? • Protists are defined by what they are NOT… – Eukaryotes that are not plants, animals, or fungi • 1st eukaryotic organisms • Autotrophic or heterotrophic • Asexual or sexual reproduction • Most are unicellular (algae exception) • Many are aquatic • “Junk drawer kingdom” 51 Protists and the Evolutionary Tree We are going to take a look at some phyla within the Protist Kingdom 52 Animal-like Protists • Unicellular • Heterotrophic organisms • Animal like protists are distinguished by how they move: 1) Sarcodines—move with pseudopods 2) Ciliates—move with cilia 3) Zooflagellates—move with flagella 4) Sporozoans—immobile 53 Sarcodines • Animal-like protists that use pseudopods for feeding and moving • Pseudopods-extensions of cytoplasm • Ex) Amoeba FOOD 54 Amoeba microscope footage Sarcodines • Ameobic dysentery • Montezuma’s revenge or “traveler’s diarrhea” Ameoba histolytica 55 Ciliates • Animal-like protists that use cilia for feeding and movement • Cilia-hair-like structures • Ex) Paramecium Paramecium microscope footage 56 Zooflagellates • Animal-like protists swim using flagella • Trypanosoma protist spread by the bite of tsetse fly causes African Sleeping Sickness • Giardia can contaminate water and cause digestive problems • Trichonympha lives in mutualistic relationship with termites 57 Sporozoans • Animal-like protists that don’t move on their own and are parasitic • Plasmodium sporozoan causes malaria • Sporozoan parasite is carried by female Anopheles mosquito 58 Plasmodium Life Cycle • Mosquito bites human and parasite injected • Parasites invade liver, reproduce, and develop • Liver cells burst and parasites move to red blood cells • RBC burst-person experiences anemia, fever, chills, may result in death • Parasites may then move into other RBC or are picked up by mosquito and transferred to another person 59 Plantlike Protists • Autotrophs- contain chlorophyll and carry out photosynthesis • Euglenophytes • Diatoms • Algae • Often called “phytoplankton”- small photosynthetic organisms near the surface of ocean • Releases tons of oxygen into the atmosphere • Important food source for many “filter feeders” 60 Euglenophytes • Plant-like protists that have flagella and chloroplast, but no cell wall. • Ex) Euglena • Autotrophs when sunny but heterotrophs when not Euglena microscope footage 61 Diatoms • Plant-like protists that produce thin, delicate cell walls made of silicon • Used in toothpaste, paints on license plates, dynamite 62 Algae • Plant-like protists – Unicellular algae – Multicellular algae • Red and brown algae • Contains special pigments that allows it to live deep areas of water • Commonly called “seaweed” (ex: Kelp) 63 Plantlike Protists: Algae • Green Algae- some are unicellular, some form colonies, few are multicellular 64 Humans and Algae • Humans understand many beneficial uses of algae: 1) Used to make nutrient agar 2) Used as ingredient in ice cream, pudding, salad dressing, syrups 3) Food source – humans and other animals 4) Releases oxygen from photosynthesis • Algae causes harm in “algal blooms” – depletes water of nutrients and oxygen 65 Fungus-like Protists • Decomposers • Heterotrophic protists that absorb nutrients, but lack cell walls with chitin – Slime molds- found near moist, rotting logs and composts – Slime mold video – Water molds – can be parasitic and cause “ick” in fish 66 PROTIST KINGDOM EUKARYOTIC UNICELLULAR AND MULTICELLULAR HETEROTROPHS AND AUTOTROPHS PLANT-LIKE AUTOTROPHIC SO CREATE OWN ENERGY SOURCE ANIMAL-LIKE FUNGUS-LIKE HETEROTROPHIC SO HAVE TO CONSUME ENERGY SOURCE, BUT ARE UNICELLULAR, UNLIKE ANIMALS HETEROTROPHIC DECOMPOSERS THAT HAVE TO CONSUME ENERGY SOURCE EXAMPLES: EXAMPLES: EXAMPLES: 67 Fungi Kingdom 68 Fungi Unicellular (yeast) Multicellular • All fungi are eukaryotic • They may be unicellular or multicellular (most) yeast 69 Fungi Characteristics • Most are immobile • All have cell walls made of “chitin”- a carbohydrate which also makes up the exoskeleton of insects CHITIN! 70 Fungal Structure • Fungi are made of thin strands called hyphae • Each strand consists of cells separated by a wall called a septa 71 Fungal Structure (cont.) • As hyphae grow, they form a tangled mass called a mycelium • The mycelium is usually underground or embedded in some food source • The main function of mycelium is to absorb food 72 Nutritional Status of Fungi • Heterotrophs because they feed off non-living, organic matter • All fungi must absorb food outside the body – Animals = ingest then digest – Fungi = digest then ingest. – Enzymes break down food outside of body (use “exoenzymes”). • Important decomposers in the ecosystem • Along with bacteria, fungi are important in recycling 73 nutrients such as carbon and nitrogen Fungal Reproduction • The part of the fungus that we see above ground is called the fruiting body • The fruiting body is the main reproductive part of the fungus 74 75 76 77 Fungal Reproduction • Fungi reproduce asexually and sexually. • Asexual reproduction can be done by… a. Hyphae breaking off and growing on their own. b. Producing spores. 78 Fungal Reproduction • Fungal spores are found in almost any environment. • Wind blows spores land in “favorable” spot new fungus. • Some fungi attract animals to aid in spore dispersal. Ex.) Stinkhorn fungus 79 Fungal Reproduction • Sexual reproduction involves two different mating types • No males or females, instead they are known as “+” plus or “-” minus. • When hyphae from two different mating types come together, they fuse together forming a diploid zygote. 80 Groups of Fungi • The main phyla of fungi are divided according to how they sexually produce spores – 1.) Zygomycetes – 2.) Ascomycetes – 3.) Basidiomycetes – 4.) Deuteromycetes 81 Zygomycetes • Ex.) Bread mold • Some of the hyphae form a zygosporangium which produces spores • Spores can be carried by wind 82 Ascomycetes (sac fungi) • Ex.) Yeast and mildew • Form spores in saclike structures called asci • Each ascus bursts open shooting spores into the air 83 Basidiomycetes (club fungi) • Ex.) Mushrooms, toadstools, bracket fungi • Spores form under the caps of mushrooms on structures called basidia 84 Deuteromycetes • Ex.) Penicillin, many disease causing fungi. • These fungi do not reproduce sexually. 85 Helpful Fungi Penicillin • Fungi can be very helpful and delicious • Many antibacterial drugs are derived from fungi • Fungi accounts for the blue vein in blue cheese! 86 Harmful Fungi • Fungi also causes a number of plant and animal diseases: •Athlete's Foot 87 Harmful Fungi • Ringworm • Fungi on discoveryed 88 89 FUNGUS KINGDOM EUKARYOTIC MULTICELLULAR HETEROTROPHS ZYGOMYCETES ASCOMYCETES FORMS ZYGOSPORANGIUM WHICH FORMS SPORES FORMS SACS CALLED ASCI THAT FORMS SPORES EXAMPLES: DEUTEROMYCETES BASIDIOMYCETES NO SPORES SPORES FORM ON BASIDIA EXAMPLES: EXAMPLES: EXAMPLES: 90