Physiological Functions of White Blood cells 1 Objectives: WBCs 1. Identify the different types of WBCs 2. Enumerate the functions of the various types of WBCs 3. Describe the steps of formation of WBCs 4. List the factors required for their maturation. 5. Infer the importance of total and differential WBC counts 6. Define the terms leucocytosis and leucopenia 7. Define leukemia 2 White Blood Cells (WBCs) Total Leucocyte count (TLC) 4000-11,000 /μL Types of WBCs Granulocytes contain granules Neutrophils Eosinophils Basophils: Agranlocytes Lymphocytes Monocytes Differential count: DLC Neutrophils 60-70% Lymphocytes 20-25% Monocytes 3-8% Eosinophils 1-4% Basophils 0-1% 3 Neutrophils 60-70% •10-12 µ in size; 2-5 lobed nucleus; Older the cell more the lobes(polymorphonuclear leukocytes); •Small colored granules which contain enzymes, Defensins, oxidants •Action: by Phagocytosis •Life span: a few hrs: 4-8 hrs in blood; 4-5 days in tissues •Fast response; neutrophilia in bacterial diseases; neutropenia in drug toxicity Eosinophils 0-4%; 10-12 µ Bi-lobed nucleus; coarse acidophilic granules Phagocytose antigen-antibody complexes; destroy parasites Granules contain various enzymes : MBP Increase in allergic conditions; decrease during stress-corticoids Basophils 0-1% 8-10 µ Nucleus usually bi-lobed; large deep purple colored granules which often cover the nucleus Secrete heparin, serotonin, histamine: help in inflammation The Granulocytes 4 Agranular WBCs Monocyte 12-20 µ;3-8%; circulate in blood for about 20-30 hrs. long life span (many weeks to months) after change into macrophages in tissues Single kidney shaped nucleus Phagocytosis after converting in to macrophages Lymphocyte 6-9 µ (small); 10-14 µ (large); 20-25%; mostly formed in lymhoid tissue; some in bone marrow. Nucleus surrounded by thin ring of cytoplasm; long life span inside tissues, but only a few hours in blood B and T lymphocytes: Immune mechanisms B lymphos form plasma cells Natural Killer (NK) cells against viruses, cancer 5 Physiology and Functions of WBCs Life span: few hours to a few days They have Major Histocompatibility Antigens on surface: -Identification of the “SELF” Main functions: combat infection by phagocytosis immune mechanisms Adhesion molecules Selectins: on endothelial cells - attach to CHOn on neutrophil surface Integrins on neutrophils: also make WBCs stick to endothelial surface, & help them to move out of the blood vessels When required , they escape from the blood via capillary pores (emigration) helped by integrins enter tissue and have their action there by phagocytosis 6 Phagocytosis Mainly by neutrophils and monocytes Steps of Phagocytosis 1 . Chemotaxis : attraction of WBCs to site of infection Promoted by i. bacterial toxins ii. kinins from damaged tissues iii. CSFs 2. Adherence phagocyte attaches to microbe using proteins 3. Ingestion pseudopods from WBC surrounds microbe formation of a PHAGOSOME 7 4. Digestion Phagosome enters cytoplasm of WBC and merges with LYSOSOME: Phagolysosome: secretion of lysozomal enzymes followed by release of oxidants: oxidative burst 5. Killing as result of the above. Residual bodies get left behind 1 CHEMOTAXIS Microbe Phagocyte 2 ADHERENCE 3 INGESTION Pseudopod Lysosome Plasma membrane 4 DIGESTION 5 KILLING Digestive enzymes Digested microbe in phagolysosome Residual body (indigestible material) Phases of phagocytosis 8 Formation of WBCs: Hemopoiesis 9 Pluripotent stem cell Lymphoid stem cell Myeloid stem cells Colony Forming Unit G/M Eosinophilic myeloblast Basophilic myeloblast • Neutrophil monocyte T lymphocyte T lymphoblast eosinophil B lymphocyte B lymphoblast NK lymphoblast basophil NK Cell Granulocytes and monocytes : formed only in the BONE MARROW stored in the bone marrow, and released into circulation. Lympocytes and Plasma cells also in lymhoid tissue, are stored in lymhoid tissue . Few released into circulation • Various Colony Stimulating factors (CSFs or Growth Inducers) help in their formation: IL1, IL6 and IL3 (multi CSF) These are proteins in nature 10 Normal WBC count: 4000-11000 Leuocytosis >11000 Leuopenia < 4000 Neutophils Infections, Burns, inflammation Lymphocytes Viral infections Monocyts Viral, fungal infections, TB, Chronic disease Some viral infections Bacterial infections (typhoid) Bone marrow suppression Drugs: antibiotics corticosteroids Eosinophils 11 Typical eg: 8 year child; high fever; pain throat; difficulty in swallowing swollen septic tonsils TLC 18,500 DLC: N. 88%; L 10%; E 2% Elderly person; exposure to radiation Hx of intractable fever and infections; Loss of appetite Leukemias (blood cancer) TLC 2000 DLC: N. 30% L 60%; E 4% ; 6% other WBCs 12 Summary 1. TLC, types of WBC, DLC 2. Description of various types of WBCs and their actions 3. Phagocytosis 4. Development of WBCs and factors associated 5. Define leucocytosis/leucopenia with typical examples 6. Leukemias or blood cancer 13