URINE SEDIMENTS DEPARTMENT OF PATHOLOGY AND LABORATORY DIAGNOSIS FATIMA COLLEGE OF MEDICINE URINE SEDIMENTS RED BLOOD CELLS. UNSTAINED, X400. URINE SEDIMENTS RED BLOOD CELLS. STERNHEIMER STAIN, X400. URINE SEDIMENTS WHITE BLOOD CELLS. UNSTAINED, X400. URINE SEDIMENTS WHITE BLOOD CELLS. STERNHEIMER STAIN, X400. URINE SEDIMENTS SQUAMOUS EPITHELIAL CELLS (SUPERFICIAL TYPE). CYTOLASM IS THIN AND WRINKLED. CELL PERIPHERY IS FOLDED. UNSTAINED, X400. URINE SEDIMENTS SQUAMOUS EPITHELIAL CELLS (SUPERFICIAL TYPE). STAINABILITY OF THE CELLS ARE GOOD. THE NUCLEI ARE SMALL AND CONDENSED. STERNHEIMER STAIN, X400. URINE SEDIMENTS SQUAMOUS EPITHELIAL CELLS (INTERMEDIATE TYPE). THE CYTOPLASM IS THICKER THAN THAT OF SUPERFICIAL TYPE CELLS. CELL PERIPHERY IS CLEAN AND ROUND. UNSTAINED, X400. URINE SEDIMENTS SQUAMOUS EPITHELIAL CELLS (INTERMEDIATE TYPE). STAINABILITY IS GENERALLY POOR. THE CELLS STAIN LIGHT PINK. STERNHEIMER STAIN, X400. URINE SEDIMENTS SQUAMOUS EPITHELIAL CELLS (DEEP LAYER TYPE). THE CYTOPLASM IS THICK AND HOMOGENOUS. CELL SHAPE IS MOSTLY SPHERICAL. UNSTAINED, X400. URINE SEDIMENTS SQUAMOUS EPITHELIAL CELLS (DEEP LAYER TYPE). STAINABILITY IS GENERALLY POOR. STERNHEIMER STAIN, X400. URINE SEDIMENTS TRANSITIONAL EPITHELIAL CELLS (SUPERFICIAL TYPE). THEY HAVE THICKER CYTOPLASM. THE PERIPHERAL IS CRESCENT- SHAPED, POLYGONAL AND ANGULAR. UNSTAINED, X400 URINE SEDIMENTS TRANSITIONAL EPITHELIAL CELLS (SUPERFICIAL TYPE). THE SURFACE STRUCTURE IS ROUGH. THE CELLS HAVE GENERALLY HAVE 2 OR 3 NUCLEI AND THEY ARE LARGE. STERNHEIMER STAIN, X400. URINE SEDIMENTS TRANSITIONAL EPITHELIAL CELLS (INTERMEDIATE TYPE). THE CYTOPLASM SHOWS A YELLOWISH TONE. SURFACE STRUCTURE OF THE CELLS IS ROUGH. THE PERIPHERAL IS ANGULAR. UNSTAINED X400. URINE SEDIMENTS TRANSITIONAL EPITHELIAL CELLS (INTERMEDIATE TYPE). STAINABILITY IS GOOD, RESULTING IN A PINK VIOLET STAIN. STERNHEIMER STAIN X400. URINE SEDIMENTS TRANSITIONAL EPITHELIAL CELLS (DEEP LAYER TYPE). THE CYTOPLASM IS GENERALLY THINNER THAN IN DEEP LAYER TYPE SQUAMOUS EPITHELIAL CELLS. THE PERIPHERY OF THE CELLS IS ANGULAR. THE UNSTAINED CYTOPLASM SHOWS A YELLOWISH TONE. UNSTAINED, X400. URINE SEDIMENTS TRANSITIONAL EPITHELIAL CELLS (DEEP LAYER TYPE). STAINABILITY IS GOOD. STERNHEIMER STAIN, X400. URINE SEDIMENTS RENAL TUBULAR EPITHELIAL CELLS. THE LARGE AND IRREGULAR CELLS ARE RENAL TUBULAR EPITHELIAL CELLS. THE NUCLEI ARE SMALL AND UNEVENLY LOCATED. UNSTAINED, X400. URINE SEDIMENTS RENAL TUBULAR EPITHELIAL CELLS. STAINABILITY IS GOOD. THE PERIPHERAL STRUCTURE IS INDENTED. STERNHEIMER STAIN, X400. URINE SEDIMENTS OVAL FAT BODY. THE FAT GRANULES SHOW A YELLOWISH BROWN GLOSS OR DARK BROWN GLOSS. UNSTAINED, X400. URINE SEDIMENTS OVAL FAT BODY. FAT GRANULES STAIN RED. SUDAN III STAIN, X400 URINE SEDIMENTS OVAL FAT BODY. THE SO-CALLED MALTESE CROSS DOUBLE REFRACTIVE IMAGE IS OBSERVED. POLARIZING MICROSCOPE IMAGE, X400. URINE SEDIMENTS HYALINE CAST. NO INCLUSION IS SEEN IN THE CAST. UNSTAINED, X400. URINE SEDIMENTS HYALINE CAST. NO INCLUSION IS SEEN IN THE CAST. STERNHEIMER STAIN, X400. URINE SEDIMENTS HYALINE CAST. CHARACTERIZED BY A SMALL QUANTITY OF FINE GRANULES INCLUDED IN THE CAST. STERNHEIMER STAIN, X400. URINE SEDIMENTS EPITHELIAL CELL CAST. MANY RENAL TUBULAR EPITHELIAL CELLS ARE SEEN IN THE CAST. UNSTAINED, X400. URINE SEDIMENTS EPITHELIAL CELL CAST. MANY RENAL TUBULAR EPITHELIAL CELLS COMPLETELY FILL THE CAST. STERNHEIMER STAIN, X400. URINE SEDIMENTS GRANULAR CAST. THE CAST CONTAINS MANY COARSE GRANULES. UNSTAINED, X400. URINE SEDIMENTS GRANULAR CAST. ROUGH GRANULES ACCOUNT FOR MOST OF THE CAST. STERNHEIMER-MALBIN STAIN, X400. URINE SEDIMENTS WAXY CAST (BROAD CAST). THE CAST SHOW A CHARACTERISTIC FISSURE. THE CAST IS VERY BROAD WHEN COMPARED WITH SURROUNDING CELLS. UNSTAINED, X400. URINE SEDIMENTS WAXY CAST. THE CAST ALSO HAS A FISSURE AND WAXY SURFACE, BUT THE WIDTH IS NOT SO GREAT. STERNHEIMER STAIN, X400. URINE SEDIMENTS FATTY CAST. FATTY GRANULES ARE TIGHTLY PACKED IN THE CAST. UNSTAINED, X400. URINE SEDIMENTS FATTY CAST. FATTY GRANULES DO NOT STAIN, BUT THE CAST AS A WHOLE, STAIN ORANGEYELLOW COLOR. STERNHEIMER STAIN, X400. URINE SEDIMENTS FATTY CAST. FATTY GRANULES STAIN ORANGE RED COLOR. SUDAN III STAIN, X400. URINE SEDIMENTS RBC CAST. THE CAST SHOW A YELLOW-BROWN COLOR REFLECTING THE HEMOGLOBIN PIGMENT. UNSTAINED, X400. URINE SEDIMENTS RBC CAST. RBC’S INCLUDED IN THE CAST STAIN LIGHT RED AND CAN BE CLEARLY DISTINGUISHED. STERNHEIMER STAIN, X400. URINE SEDIMENTS WBC CAST. THIS CAST CONTAINS MANY WBC’S. UNSTAINED, X400. URINE SEDIMENTS WBC CAST. THIS CAST CONTAINS MANY WBC’S INCLUDING SEGMENTED NEUTROPHIL. STERNHEIMER STAIN, X400. URINE SEDIMENTS BACTERIA. MANY ROD-SHAPED BACTERIA ARE OBSERVED. WBC’S ARE ALSO PRESENT. THIS SPECIMEN IS REPORTED AS “BACTERIA (+++)”. URINE SEDIMENTS YEAST. THE SHAPES ADOPTED BY YEAST ARE OVOID, GRAINY AND BUDDING. THIS SPECIMEN IS REPORTED AS “YEAST (+++)”. URINE SEDIMENTS TRICHOMONAS. FLAGELLATED TRICHOMONAS WHICH MOVE ACTIVELY ARE SEEN. THEY ARE GLOSSY AND A LITTLE LARGER THAN WBC. THIS SPECIMEN IS REPORTED AS “TRICHOMONAS (+++)”. URINE SEDIMENTS CALCIUM OXALATE CRYSTALS. COLORLESS, REGULAR OCTAHEDRAL. PH 6.0. UNSTAINED, X400. URINE SEDIMENTS CALCIUM OXALATE CRYSTALS. BISCUIT-SHAPED. THEY MAY BE CONFUSED WITH RED BLOOD CELLS. PH 6.0, UNSTAINED, X400. URINE SEDIMENTS URIC ACID CRYSTAL. THEY APPEAR YELLOWBROWN IN COLOR. IN THIS EXAMPLE, LARGE LENS-SHAPED CRYSTALS OVERLAP EACH OTHER. PH 6.0, UNSTAINED, X400. URINE SEDIMENTS URIC ACID CRYSTAL. NOTICE THE YELLOW COLOR AND VARIETY OF SHAPES. X400. URINE SEDIMENTS URIC ACID CRYSTAL, POLARIZED. X400. URINE SEDIMENTS CALCIUM PHOSPHATE CRYSTAL. THIS IS WHITISH GRAY, IRREGULAR PLATE FORM. PH 8.0, UNSTAINED, X400. URINE SEDIMENTS TRIPLE PHOSPHATE CRYSTAL. COLORLESS CRYSTALS CHARACTERIZED BY STRONG BIREFRINGENCE AND ENVELOPE OR COFFIN FORMS. PH 7.5, UNSTAINED, X400. URINE SEDIMENTS BILIRUBIN CRYSTALS. REDDISH BROWN, SHORT NEEDLE-SHAPED CRYSTALS FORMING BUNDLES. PH 6.5, UNSTAINED, X400. URINE SEDIMENTS CHOLESTEROL CRYSTALS. COLORLESS FLAT PLATES WITH NOTCHED EDGES. A CASE OF CYSTIC NEPHROPATHY. UNSTAINED. URINE SEDIMENTS CYSTINE CRYSTALS. HEXAGONAL PLATES. PH 5.5, UNSTAINED, X400. URINE SEDIMENTS AMMONIUM BIURATE CRYSTALS. NOTICE THE “THORNY APPLE” APPEARANCE, X400. URINE SEDIMENTS TYROSINE CRYSTALS. YELLOW COLOR SUGGESTS LIVER DISEASE. X400. URINE SEDIMENTS SULFA CRYSTALS. ALSO SEEN ARE WBC’S AND BACTERIA SUGGESTING A UTI. X400. URINE SEDIMENTS AMPICILLIN CRYSTALS. BUNDLES OF CRYSTALS ARE SEEN AFTER REFRIGERATION. X400. URINE SEDIMENTS STARCH GRANULES. NOTICE THE REFRACTILITY. X400. URINE SEDIMENTS NORMAL SPERMATOZOA. X400. URINE SEDIMENTS TRANSITIONAL CELL CARCINOMA CELLS. THE CELLS APPEAR IN MASSES AND ARE MULTILAYERED. UNSTAINED, X400. URINE SEDIMENTS TRANSITIONAL CELL CARCINOMA CELLS. HIGH N/C RATIO, PROMINENT HYPERCHROMASIA AND ANISOKARYOSIS ARE SHOWN. A CASE OF URINARY BLADDER CA. STERNHEIMER STAIN. X400. URINE SEDIMENTS ADENOCARCINOMA CELLS. HIGH N/C RATIO, ENLARGED NUCLEOLI AND ANISOKARYOSIS ARE SHOWN. A CASE OF MAMMARY CA WITH METASTASIS TO KIDNEY, STERNHEIMER STAIN, X400. URINE SEDIMENTS SQUAMOUS CELL CARCINOMA CELLS. STRANGE SHAPES, NUCLEAR ENLARGEMENT, HYPERCHROMASIA AND ANISOKARYOSIS ARE SHOWN. A CASE OF CONTAMINATED URINE WITH SQUAMOUS CELL CARCINOMA OF THE UTERINE CERVIX. STERNHEIMER STAIN, X400. THANK YOU!!!