Breeding SHB Cultivars at UF-IFAS

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Breeding SHB Cultivars at UF-IFAS
Dr. Jim Olmstead
Horticultural Sciences Department
FBGA Fall Meeting – September 19, 2013
UF Blueberry Breeding History
• 1950s: Ralph Sharpe begins blueberry
breeding at UF
• 1976: Wayne Sherman and Ralph Sharpe
release ‘Sharpblue’ and ‘Flordablue’ – first
southern highbush varieties
• 1990s: Plantings increased with improved
varieties released by Paul Lyrene.
• 2000s: Rapid expansion of Florida industry
UF Blueberry Breeding Strategy
• Recurrent selection
• High density nursery, rapid breeding cycles
• Large parent, cross, and seedling numbers
Year
1 2 3 4 5 6 7 8 9 10 11 12
• Up to 200 crosses per year
• Elite parents (cultivars and
advanced selections)
• Top seedling performers
• Wild germplasm
UF Blueberry Timeline
UF Blueberry Timeline
Year
1 2 3 4 5 6 7 8 9 10 11 12
UF Blueberry Timeline
Year
1 2 3 4 5 6 7 8 9 10 11 12
Stage 1
Precocious
Early maturity
Large size
Good color
Good stem scar
Firmness
Flavor
2009 Stage 1
Year
1 2 3 4 5 6 7 8 9 10 11 12
2007 Stage 2
2008 Stage 2
Stage 2
Survival
Chill adaptation
Crop potential
Early maturity
Large size
Good color
Good stem scar
Firmness
Flavor
2009 Stage 1
Year
1 2 3 4 5 6 7 8 9 10 11 12
Stage 3 –
Cooperator trial
• 200+/year
• 15 plant plots
• Survivability
2008 Stage 3
2007 Stage 3
2006 Stage 3
2009 Stage 3
Year
1 2 3 4 5 6 7 8 9 10 11 12
Advanced Selection Trial Sites
Windsor/Waldo, FL
Interlachen, FL
Citra, FL
Haines City, FL
Arcadia, FL
Stage 4 –
• 20/year
• 5-50 plant plots
• Management systems
• Yield trials
• Fruit quality measurements
Field Ratings (2011-2013)
Genotype
Proposed
Emerald
Scintilla
Star
Sweetcrisp
Farthing
Meadowlark
Primadonna
50% Bloom
Date (DOY)
44
37
31
49
41
45
34
41
Harvest
Timing
Early-mid
Mid-late
Early-mid
Mid
Late
Mid-late
Early
Early-mid
Surv.
7
8
7
7
9
9
7
8
Yield Leaf. Size Color
7
7
8
6
8
8
9
7
6
7
8
9
6
6
7
7
5
6
5
7
9
5
6
7
7
7
7
8
4
6
6
6
Scar Firm. Flavor
8
9
8
7
8
6
8
7
9
7
7
7
9
9
8
9
9
6
6
8
7
7
6
8
Lab Ratings (2012)
Avg. Berry
% Titrat.
% Soluble
SS/TA
Firmness
Genotype
Wt. (g)
Acidity
Solids
Ratio
(g/mm)
Proposed
2.41 ± 0.24
0.472 ± 0.16
11.5 ± 0.92
26.6 ± 9.1
247.94 ± 16.9
Emerald
2.40 ± 0.21
1.106 ± 0.15
9.1 ± 2.10
8.6 ± 3.4
183.32 ± 17.2
Scintilla
2.68 ± 0.26
0.746 ± 0.28
12.8 ± 2.30
19.8 ± 10.1
231.97 ± 6.5
Star
2.28 ± 0.42
0.851 ± 0.23
9.8 ± 2.65
11.9 ± 4.0
217.96 ± 14.7
Sweetcrisp 2.31 ± 0.08
0.842 ± 0.07
12.5 ± 1.33
14.9 ± 1.6
262.29 ± 37.5
2.34 ± 0.26
0.688 ± 0.13
10.5 ± 1.93
15.8 ± 4.7
230.49 ± 14.2
Meadowlark 2.67 ± 0.25
0.461 ± 0.10
11.3 ± 0.68
25.3 ± 5.6
233.06 ± 7.2
Farthing
Harvest Timing/Yield
Total pounds/week for 5 bushes
(3-yr old planting)
Week of Year
Selection 1 Selection 2
Jewel
5
3.00
0
0
6
0
0
0
7
1.19
0
0
8
0.88
0
0
9
0
0
0
10
3.63
0.07
0
11
2.75
0.07
0
12
5.38
0.44
0
13
3.76
1.00
0
14
4.32
5.51
5.69
15
4.82
8.57
8.45
16
2.69
7.94
16.7
17
3.44
6.44
4.50
18
4.19
11.57
8.69
19
1.38
3.38
0.88
Total (Pound/5 bush)
41.43
44.99
44.91
Pound/acre (1800 plants) 14,900
16,100
16,100
Sensory Evaluations
Anne Plotto and Liz Baldwin – USDA
Year
1 2 3 4 5 6 7 8 9 10 11 12
UF Variety Release
• Must adhere to current UF-IFAS standards
- Tested in enough replication, locations,
years, etc.
- Chain of custody – developmental
timeline, MTA agreements
- Foundation stock
- Plan for intellectual property protection
and commercialization
UF Variety Release
• Cultivar Release Advisory Committee
- Departmental, comprised of research and
Extension
• Cultivar Release Committee
- IFAS
Once the variety is approved by these two
committees it is considered “Released”.
Variety is commercially available only when
licenses for propagation have been offered.
Year
1 2 3 4 5 6 7 8 9 10 11 12 13
UF Variety Release
• After release, Florida Foundation Seed
Producers, Inc. handles commercialization for
the University of Florida.
• Patent/trademark application filed
• Licensing strategy developed
• Licenses for propagation in Southeastern U.S.
offered
• Upon request for other territories worldwide,
Invitation to Negotiate (ITN) is considered
Royalties
• UF – IFAS policy on release, protection and
introduction of plant material:
70% of the royalty payment comes directly
back to the breeding program
Royalty payments fund personnel, students,
and infrastructure
Dr. Jim Olmstead
UF-IFAS Horticultural Sciences Dept.
2211 Fifield Hall
Gainesville, FL 32611
(352) 273-4837
http://www.hos.ufl.edu/faculty/jwolmstead
UF Blueberry Breeding and Genetics
David Norden – Research Coord.
Werner Collante – Lab Manager
Paul Lyrene – Professor Emeritus
Rachel Itle – Postdoctoral Assoc.
Sarah Taber – Postdoctoral Assoc.
Patty Rodriguez – PhD Student
Gerardo Nunez – PhD Student
Jessica Gilbert – PhD Student
Catherine Cellon – MS Student
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