Sustaining the supply of high quality blueberry by leveraging applied breeding, genetics and genomics Hamid Ashrafi North Carolina State University Department of horticultural Science hamidashrafi@ncsu.edu Blueberry, is one of the most economically important crops in the dicot family Ericaceae. It is a nutrient dense small fruit consumed in the United State and elsewhere in the world. North Carolina is ranked 5th in the nation for highbush blueberry production with the annual cash price of about $70M on 5,500 acres. Blueberries are mainly grown in the southeast and coastal areas of NC due to better soil adaptability (sandy and acidic soil) and favorable environment. In 2015, the total production of blueberries in NC was at 14,832 mT (33,700,000 lb) of conventional fresh blueberries and 167 mT (370,000 lb) of organic fresh blueberries. North Carolina State University has a long history of blueberry breeding and variety development. In the early 1930s, collaboration with USDA in New Jersey started, mainly due to loss of cultivars that were infected by stem canker fungus. The causal agent of stem canker is the fungus Botryosphaeria cortices, which is native to the coastal plain of the southeastern US. The first stem canker resistant cultivar developed was Wolcott in the 1950s. Wolcott’s resistance broke down quickly and it was replaced by Croatan. In the 1970s, the introduction of southern highbush cultivars played an important role in NCSU blueberry breeding. Two cultivars, O’Neal and Reveille are among the most successful southern highbush cultivars introduced since then. The improved adaptation and superior fruit quality of southern highbush types continues to dominate breeding efforts today (J. A. Ballington Pers. Comm.). North Carolina State University has a successful track record of releasing blueberry cultivars. Until recently all breeding efforts at NCSU blueberry breeding program were mainly focused on traditional breeding which has been resulted in the release of 11 standard highbush, 16 southern highbush, 11 rabbiteye, 2 pentaploids and 4 ornamental blueberries. With the advent of modern breeding methods and sequencing technologies the conventional breeding can be practiced in a more efficient and faster pace. Development of DNA markers associated with desirable traits and application of marker assisted selection can facilitate development of new varieties in a shorter time. This talk aims to introduce the past, present and future trends of the program to audience and to demand for identifying collaborators across all disciplines to breed for higher yield, higher quality, disease and pasts resistant, and machine harvest adapted blueberries.