Human Lactoferrin
•Lactoferrin is a glycoprotein secreted by mucosal membranes; it
is a major protein in the secondary granules of leukocytes
•During intestinal inflammation, activated leukocytes infiltrate the
mucosa and lumen, increasing the level of fecal lactoferrin
•Fecal lactoferrin is elevated in patients suffering from active
inflammatory bowel disease (IBD) but not in persons with irritable
bowel syndrome (IBD) or healthy persons
Lactoferrin levels in disease
Ulcerative
Colitis
N=71
Lactoferrin levels
(ug/g wet weight)
1200
800
400
0
Crohn’s
Disease
N=78
Irritable
Bowel
Syndrome
N=31
Healthy
Persons
N=56
Two new non-invasive tests to
detect intestinal inflammation
• IBD-CHEKTM – a qualitative ELISA for the clinical lab
• IBD-QUIK CHEKTM – a qualitative lateral flow test for
the clinical lab or physician’s office
Performance Characteristics of
the IBD-CHEK™ Test
N=236
Total
IBD-CHEKTM
IBD-CHEKTM
(+)
(-)
Crohn’s disease
Active
Inactive
52
26
85%
62%
15%
39%
Ulcerative colitis
Active
Inactive
40
31
88%
52%
13%
48%
Active IBS
31
0%
100%
Healthy persons
56
0%
100%
Clinical Results with the
IBD-CHEK™
• When distinguishing active IBD from irritable
bowel syndrome and healthy persons, the
IBD-CHEKTM test exhibited a sensitivity of
86% and specificity of 100%
• When distinguishing active Crohn’s disease
from irritable bowel syndrome and healthy
persons, the IBD-CHEKTM test exhibited a
sensitivity of 85% and specificity of 100%
IBD-CHEK™ Test
• Qualitative ELISA for detecting elevated fecal
lactoferrin as an indicator of intestinal inflammation
• Utilizes a four-step procedure with results in 75
minutes
• A valuable noninvasive diagnostic aid to distinguish
active IBD from IBS
• Available by direct order for hospital and reference
labs