GalliPro
Bacillus based probiotic
Bacillus spore ideal as probiotic for chicken
GalliPro
Comprise live Bacillus spore – natural encapsulated bacteria
Survive feed pelleting process and in feed storage
Has compatibility with all EU approved coccidiostats
Transform from spores to active cells in the intestine of chicken
Reduce FCR
Increase weight gain
Increase nutrient utilization by excretion of enzymes
Improves intestinal health and absorption capacity
Modulate intestinal microflora resulting in more Lactic Acid
Bacteria
Reduce Salmonella and Campylobacter
Bacillus sp.
Definition of Bacillus
Gram positive organisms
Spore formers
Bacillus is facultative aerobe but at present of nitrate/nitrite it
can grow anaerobic
Bacillus is found in soil and marine sediments
Bacillus as probiotic is in the form of Bacillus spore – a dormant
resistant stage that can transform to a vegetative cells
Bacillus spores are
pH stable at pH 2-3
Trypsin stable
Thermo-tolerant
In-Feed stable
Bacillus spores the ideal probiotic for chicken?
Pelletizing study on selected probiotic strains on the market
•Pelletizing at 85C 30 sec.: External facility, Bioteknologisk Institut, Denmark
•Microbiological analysis: External lab, LUFA –ITL-GmbH, Germany
Result: Viable cell count in average CFU/g feed and the recover of viable
Calculated
CFU/g
Meal feed
CFU/g
Pellet feed
CFU/g
Recover of cells after
pelleting, %
Control
total aerobic
NA
2.6E+06
3.6E+04
1
L.farcimis
NA
3.1E+04
< 10
<1
P.acidilactici
4.0E+06
3.6E+06
2.7E+04
1
E.faecium
4.0E+06
8.5E+06
1.5E+06
18
S.cereviciae
8.0E+06
8.0E+06
2.7E+04
<1
B.licheniformis
1.3E+06
1.2E+06
1.2E+06
100
GalliPro
1.3E+06
1.2E+06
1.2E+06
100
Sample ID
Bacillus spores the ideal probiotic for chicken?
Bacillus spore extremely resistant – easy to apply into feed
Bacillus spore germination occurred rapidly in the GI tracts of
chicken (Cartman et al 2008)
Vegetative cells can be detected throughout the GI tract of chicks
(Cartman et al 2008)
Feeding Bacillus subtilis to chicken resulted in greater growth
performance and intestinal histologies such as increasing villus
height. Villus tip showed no damage (Samanya & Yamauchi 2002)
Indicating improved nutrient utilization capacity
Bacillus spores the ideal probiotic for chicken?
Feeding GalliPro
More diverse and complex bacteria composition in the gut
Expectedly provides more robust microbiota less susceptible towards
diseases and infections (Lan et al 2005)
High numbers of Lactic Acid Bacteria
Considered beneficial due to their putative health properties – protect
against pathogens colonization and immune stimulation of the host
Lactobacillus salivarius
Strong probiotic characteristics reducing Salmonella and E. coli (Pascual et al
1999, Brashears et al 2003)
Lactobacillus paracasei
Stimulate the immune system of the host (Herich et al 2002, Morelli et al
2003)
GalliPro reduces the level of Campylobacter in
broiler chickens
Quantification of Campylobacter on caecal samples
4,50E+07
4,00E+07
3,50E+07
cfu/g
The result shows a significant
reduction of Campylobacter
by 52% in the GalliPro
treated group.
3,00E+07
2,50E+07
2,00E+07
1,50E+07
1,00E+07
5,00E+06
The results on ileal samples
showed a 40% reduction of
Campylobacter.
Gallipro group
Control group
Quantification of campylobacter in ileum samples
CFU/g
Our finding of Campylobacter is
accordance with literature. Corry
and Atabay (2001) reported the
commonly observed level in bird
intestinal to be 105 to 107 CFU/g.
0,00E+00
1,00E+04
9,00E+03
8,00E+03
7,00E+03
6,00E+03
5,00E+03
4,00E+03
3,00E+03
2,00E+03
1,00E+03
0,00E+00
gallipro group
control group
GalliPro significantly reduce Salmonella in chicken
Salmonella challenge pen floor
study (SPR, US) with and
without GalliPro showed a
significantly reduction of
Salmonella in the GalliPro
treated group
% Salmonella positive
120
100
a
80
60
All samples were found positive
at day 42 in the control group
whereas only 43% of the
samples in the GalliPro group
were positive with regards to
Salmonella
b
40
20
0
Control
GalliPro
Different letters indication significant difference P< 0.05
Bacillus spores the ideal probiotic for chicken?
GalliPro produce enzymes
Alcalin phosphatase
Esterase
Esterase lipase
Lipase
Leucine amino-peptidase
Valine amino–peptidase
Acid phosphatase
Glucosidase
Bacillus shows amylolitic (starch), lipolytic (fat) and proteolytic (protein)
activities
Summary of 17 trials
GalliPro vs. Negative Control
Relative efficacy of GalliPro in broiler trials at independent research institutes
Avg. improvement: Final bodyweight +2,8% & FCR -3,8%
+2,8%
-3,8%
Big increases in in-vivo nutrient utilisation
ME 100% diet
Control
GalliPro
Diff
Dry matter ileal digestibility (%)
74,1
78,0
3,9
Crude protein ileal digestibility (%)
82,6
85,6
3,0
Gross energy ileal digestibility (%)
77,0
81,0
4,0
N retention (%)
66,6
68,8
2,2
ME (MJ/kg DM)
13,88
14,19
0,3
ME (MJ/kg DM, N corrected)
13,46
13,76
0,3
GalliPro increased in-vivo dry matter, crude protein and gross energy digestibilities, N
retention and metabolisable energy.
Study 2 at University Federal de Viçosa, Brazil
GalliPro Works!
Increased in-vivo DM, CP & GE
digestibilities
Increased weight gain, improved FCR
and lower mortality
Canadian & EU Registration
Safe, stable and easy to use
Consistent performance
Bacillus spores the ideal probiotic for chicken?
Bacillus subtilis (DSM17299) compatibility with Coccidiostats:
Amprolium/ethopabate
Diclazuril
Halofuginone
Lasalocid sodium
Maduramicin ammonium
Methylbenzoquate/meticlorpindol
Monensin sodium
Narasin
Nicarbazin
Nifursol
Robenidine
Salinomycin sodium
Semduramicin