BACILLUS CEREUS A virtual case study. A stroll in the park, a sprint to the bathroom. • A family picnic with rice and salad dishes amongst others. • Many family members fell ill with diarrheal and vomiting symptoms within a few hours of consuming food. • It is determined that both the rice and the salad were the source of the illness. • A faeces sample is processed at QML for diagnostic study. A profile of Bacillus cereus. • A gram-positive endospore former, common in soil and • • • • • present of many foods derived from ground based agriculture such as rice and salad vegetables. Produces vomiting symptoms from as little as half an hour after inoculation. Diarrheal symptoms after 6 hours. A close similarity to the onset of the much more severe clostridiosis has been noted. Pathophysiology caused primarily by the production of toxins. Spore formation allows B.cereus to thrive in high heat situations as well as some strains ability to continue activity under refrigeration. The diagnostic pathway of Bacillus cereus Appearance of B.cereus on PEMBA agar. http://pages.usherbrooke.ca/biomedias/cultures_microbio.htm • Note the motile appearance of the colonies. Some confirmatory tests • Left – a motile strain of Bacillus lentimorbus. • Right – a non motile colony growth. The catalase test. http://ericaandkevin.pbworks.com/w/page/5827086/Gram%20Stain%20and%20Other%20Tests The future of laboratory diagnosis. • The Bacillus genus is very • • • • genetically conserved, even the 16S ribosomal DNA sequences. It is important to distinguish between pathogenic and commensal strains. Current methodology is unable to do this,. The molecular revolution has made this a viable future routine form of diagnostic. The toxin profile of the strain is the most important. Phylogenic tools. References • Fatal Family Outbreak of Bacillus cereus-Associated Food Poisoning Katelijne et.al J. Clin. Microbiol. August 2005 43:8 4277-4279; doi:10.1128/JCM.43.8.4277-4279.2005 • Ehling-Schulz M and Messelhäusser U (2013) Bacillus “next generation” diagnostics: moving from detection toward subtyping and risk-related strain profiling. Front. Microbiol. 4:32 • Bacteriological Analytical Manual Chapter 14 Bacillus cereus 2012 Sandra M. Tallent et.al http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070875.htm