Biomaterials and Tissue Engineering Laboratory
Liu HW Lab
Fu Jen Catholic University
組織再生
Tissue Regeneration
劉席瑋
Hsia-Wei Liu
輔仁大學生命科學系助理教授
中研院物理所訪問學者
工研院材化所研究員
長庚醫院骨科博士後研究員
國立清華大學化工博士/醫學工程碩士
全球生技產業概況---全球醫療趨勢
我國生技產業現況
甚麼是再生醫學?
甚麼是組織工程?
構成個體的層次
循環系統
呼吸系統
消化系統
泌尿系統
骨骼肌肉系統
免疫系統
神經系統
內分泌系統
生殖系統
皮膚系統
Why is it Important?
• Birth Defects (genetic mutations)
Why is it Important?
• Burn Victims
Why is it Important?
• War Injuries
Why is it Important?
• Sports Injuries
器官，不要再等了！
侏儸紀公園
When mosquito meets dinosaur 
AMBER
?
?
?
I am there
17
?
幹細胞(Stem Cell)
A cell that has the ability to continuously
divide and differentiate (develop) into
various other kind(s) of cells/tissues
Stem cells are the base cells of the body. The different tissues
form out of them – skin, muscles, bones, blood, nerve fibres,
and all the organs.
Stem Cell Transplant Research,
Parkinson's Disease
Stem cells used to boost breasts
明尼蘇達大學這批科學家由朵瑞絲泰勒博士（Dr. Doris Taylor）領軍，他們
從捐贈者遺體取出心臟，在實驗室以界面活性劑洗去心臟細胞，取得有「鬼
心」（ghost heart）之稱的膠原蛋白支架。再將取自需換心者的數百萬顆年輕
幹細胞注入支架，假以時日，這群幹細胞會附著上支架、分裂至特定數量，
心臟即能自主搏動。
使年輕;使恢復精神
組織工程基本原理
Basic principles of tissue engineering
Cells expansion
Growth Factors
Cells isolation
Biomaterials
3-D Scaffold
In vivo implantation
In vitro culture
組織工程三要素
細胞
支架
生長因子
幫細胞蓋一個家…組織工程用支架
Scaffolds
• Various textures and
materials
• Encourage cells to grow
• Allow nutrients to permeate
• Won’t harm the patient
Tissue Engineering
• Generation of semi-synthetic tissues
and organs
– Biocompatible scaffolding materials
• Synthetic polymer or natural material
(e.g. collagen)
– Living cells grown in culture
• Fully differentiated cells
 From simple tissues and organs (skin, cartilage, urinary
bladders) to whole organs using stem cells
Skin/cartilage
Drug Delivery
Devices
Polymers
Bone
replacements
Orthopedic
screws/fixation
Metals
Ocular
implants
Synthetic
BIOMATERIALS
Ceramics
Dental Implants
Implantable
Microelectrodes
Heart
valves
Dental Implants
Semiconductor
Materials
Biosensors
Biomaterials cover all classes of materials
– metals, ceramics, polymers
Uses of Ceramics
• Structural components
– Joint replacements, hip and knee
– Spinal fusion devices
– Dental crowns, bridges, implants,
etc
• Other applications
– Inner ear and cochlear匙形
implants
– Tissue engineering
– Coatings for heart valves
Engineering bone grafts
• Change stem cells
into bone cells
– with proper
growth factors in
cell culture media
• This scaffold can’t
be too big or the
cells inside will die
since they will not
get enough
oxygen
A 3D calcium phosphate scaffold
From Becton Dickinson
Uses of Metals
• Structural components
– Joint replacements
– Bone fracture pins, plates
– Dental implants
• Other applications
– Leads, wires, tubing
– Cardiac devices
Artificial Hip Joints
http://www.totaljoints.info/Hip.jpg
Uses of Polymers
• Many applications
– Valves, ducts, catheters
– Joint replacement
– Coatings, encapsulants
– Tissue engineering scaffolds
– Lenses
Intraocular Lens
3 basic materials - PMMA, acrylic, silicone
Vascular Grafts
Uses of Biological Materials
• Scaffolds
• Replacement tissues
– Skin, cartilage, etc.
• Coatings for synthetic
polymeric or metallic
materials
• Plastic/cosmetic
surgery
Substitute Heart Valves
Artificial Vessels
• Usually for by-pass
operation
http://popularmechanics.com/popmec
h/sci/tech/9805TUMDOM.html
A new bladder
• To make a bladder one
need a scaffold and
several different type of
cells
Bone repair
• Obtain patient’s bone
cells
– From the hip
• Seed onto scaffold
• Implant scaffold
– Use body as incubator
– All appropriate growth
factors available
• Remove and implant in
proper location
Replacement cells for diabetes
In vivo Islet of Langerhans in pancreas
Replacement cartilage
A close up image of cells in the replacement cartilage
• Mimic usual cartilage
environment
– Cartilage cells
– Collagenous scaffold
– Doesn’t require extensive
blood supply
組織工程眼角膜
組織工程皮膚
軟骨再生
Cartilage Regeneration
Evolution of Biomaterials
Structural
Soft Tissue
Replaceme
nts
Functional Tissue
Engineering
Constructs
Advances in
Biomaterials Technology
• Cell matrices for 3-D growth and tissue
reconstruction
• Biosensors, Biomimetic , and smart
devices
• Controlled Drug Delivery/ Targeted
delivery
• Biohybrid organs and Cell
immunoisolation
– New biomaterials - bioactive,
biodegradable, inorganic
– New processing techniques
Biomaterials - An Emerging
Industry
• Next generation of medical
implants and therapeutic modalities
• Interface of biotechnology and
traditional engineering
• Significant industrial growth in the
next 15 years -- potential of a
multi-billion dollar industry
Q&A