Data Collection: Leaf Size Time frame: Weeks 1-4 Collection frequency: Once or twice weekly widest diameter Cotyledon First true leaves Rosette Data Collection: Timing of Bolting How many days old is the plant when the bolt launches? Time frame: Approximately days 21-29 Collection frequency: Once for each plant The bolt will start growing (“launch”) from the center of the rosette. This typically occurs between the third and fourth week of growth. Data Collection: Number of Rosette Leaves at Bolting How many rosette leaves does each plant have when the bolt launches? Time frame: Approximately days 21-29 Collection frequency: Once for each plant Once the bolt launches the number of rosette leaves typically will not increase. Count the number of leaves on the rosette, not including the little cotyledons. Data Collection: Bolt Height Measure the bolt height during growth and at its maximum Time frame: Weeks 4-6 Collection frequency: Once or twice weekly Measure from the tip of the bolt to the point where it meets the rosette. Data Collection: Reproductive yield Count the total number of seed pods (siliques) when plants have stopped flowering. Only count siliques if they are 5mm or longer. Time frame: Approximately at 6 weeks. Collection frequency: Once for each plant Data Collection: Color Are the mutant plants a different color than the wild-type plants? • Students can use color charts or paint chips to compare plant color. • They can also put the mutant and wild-type plants side-by-side and take a photo of them with a digital camera. • Make sure photos include the labels on the pots so it is clear which plants are shown.