Bromothymol Blue - Port Fest Baltimore 2015

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Bromothymol Blue
Turns blue when
mixed with
OXYGEN
Turns light yellow
when mixed with
CARBON
DIOXIDE.
Why is there a
straw in the
yellowish
beaker?
Exercise Lab
Prelab
(Reactants of cellular respiration)
Oxygen + Sugar
(Products of cellular respiration)
Carbon Dioxide + Water + ATP
Energy
Define Metabolism/Metabolic Rate: The speed at
which an organism makes energy from sugars.
Scientific Question: Does sitting or exercising release
more Carbon Dioxide (CO2)?
Hypothesis:
Make your own before going into the lab! What do
you think will happen?
Exercise Lab
Prelab Continued
Scientific Question: Does sitting or exercising release
more Carbon Dioxide (CO2)?
Hypothesis:
Make your own before going into the lab! What do
you think will happen?
Variables & Procedures:
Independent Variable: I am testing… What activity releases
more CO2.
Dependent Variable: I am measuring… What activity will turn
bromothymol blue into a yellow color faster?
Controls: Why is using the same person during the
experiment important? Use METABOLISM in your answer.
Safety:
SAFETY!! You will be using a plastic bag at the
top of your container to blow into the
bromothymol blue. DO NOT LET ANY B. BLUE
GO INTO YOUR MOUTH!! While it is not
poisonous it DOES NOT taste good! Let me
know if you make a mistake and do so.
You will blow into the b. blue when you are
resting. Count how long it takes for the b.
blue to turn light yellow (Use stop watch from
phone)
Exercise and repeat the process.
Complete two trials for each type of activity.
**Only 1 person from each group is allowed to
have a phone out for using stop watch.
After watching and listening to me please write
down the procedures in your own words.
Basically, I just need to see that you’ve been
paying attention and you know what the lab is
about
Exercise Lab
Procedures
Exit Quiz
Explain in your own words how the products and
reactants of photosynthesis and cellular
respiration form a continuous cycle.
Use the following words:
1. Energy (light)
2. Energy (ATP)
3. Carbon dioxide (CO2)
4. Oxygen (O2)
5. Water
6. Sugar (glucose/carbohydrate)
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