Bromothymol Blue - Port Fest Baltimore 2015

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Bromothymol Blue

Turns blue when mixed with

OXYGEN

Turns light yellow when mixed with

CARBON

DIOXIDE.

Why is there a straw in the yellowish beaker?

Exercise Lab

(Reactants of cellular respiration)

Prelab

(

Products of cellular respiration)

Oxygen + Sugar

Carbon Dioxide + Water + ATP

Energy

Define Metabolism/Metabolic Rate: The speed at

which an organism makes energy from sugars.

Scientific Question: Does sitting or exercising release

more Carbon Dioxide (CO

2

)?

Hypothesis:

Make your own before going into the lab! What do you think will happen?

Exercise Lab

Prelab Continued

Scientific Question: Does sitting or exercising release

more Carbon Dioxide (CO

2

)?

Hypothesis:

Make your own before going into the lab! What do you think will happen?

Variables & Procedures:

Independent Variable: I am testing… What activity releases more CO2.

Dependent Variable: I am measuring… What activity will turn bromothymol blue into a yellow color faster?

Controls: Why is using the same person during the experiment important? Use METABOLISM in your answer.

Safety:

SAFETY!! You will be using a plastic bag at the top of your container to blow into the bromothymol blue. DO NOT LET ANY B. BLUE

GO INTO YOUR MOUTH!! While it is not poisonous it DOES NOT taste good! Let me know if you make a mistake and do so.

You will blow into the b. blue when you are

resting. Count how long it takes for the b. blue to turn light yellow (Use stop watch from phone)

Exercise and repeat the process.

Complete two trials for each type of activity.

**Only 1 person from each group is allowed to

have a phone out for using stop watch.

After watching and listening to me please write down the procedures in your own words.

Basically, I just need to see that you’ve been paying attention and you know what the lab is about

Exercise Lab

Procedures

Exit Quiz

Explain in your own words how the products and reactants of photosynthesis and cellular respiration form a continuous cycle.

Use the following words:

1. Energy (light)

2. Energy (ATP)

3. Carbon dioxide (CO

2

)

4. Oxygen (O

2

)

5. Water

6. Sugar (glucose/carbohydrate)

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