LAB 2: PREPARING TO CLONE
THE RFP GENE: DIGESTING THE
PKAN-R AND PARA
Pre-lab activity
DNA
isolation
Clone
Transform
Express
Purify
Lab 2: Preparing to clone the RFP gene: digesting
the pKAN-R and pARA
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Title goes at the top of the first page
Each section must be clearly underlined
Skip lines between each section
Use blue or black PEN!
Follow these directions. You must be ready
for lab (including pre-lab questions) at the
beginning of the period.
• 4. Objective: Read the background
information and determine the objective of
the lab.
• 5. Hypothesis: Create your hypothesis.
• 6. Materials: copy the materials in a
bulleted list
• 1. Title: copy this title..." Preparing to
clone the RFP gene: digesting the
pKAN-R and pARA ”
• 2. Background: write a definition for the
following words: plasmids, gene
expression, selectable markers, pARA,
pKAN-R, RNA polymerase, beta
lactamase, ampicillin, fluorescent protein,
restriction site.
• 3. Pre-lab questions: Answer the STOP
and THINK question on page B-13.
• 7. Procedure: Create a flowchart of the
procedure.
• 8. Data : Take a picture of your tubes. Print
and label.
• 9. Data Analysis: Copy and answer the
STOP & THINK questions on page B-15 &
B-16.
• 10. Conclusion: Copy & answer the 5
questions page B-17. (Do this after lab is
over).