Amgen Biotech Experience
Before the lab on Friday.
 Read A5-A13 (7-15). Introduction: What is Genetic Engineering.
o Answer the “What do you already know?” ?’s in your lab book
 Read A17-A21. (19-23)
o Copy the Laboratory 1.1: How to Use a Micropipette materials and
methods into your lab book.
Before the lab on Monday.
 Read A22-A23. (21-23) The Genetic Engineering Process.
 Read A24-A29. (24-31)
o Copy the Laboratory 1.2: Gel Electrophoresis materials and methods
into your lab book.
Before the lab on Tuesday.
 Download, Print and Read the Kitty Paternity lab.
 Determine who you think the potential daddy kitty might be based on your
phenotypic observations.
 Bring the lab paperwork to class with you.
Before the lab on Wednesday.
 Read B3-B11 (37-45)
o Do the activity “Clone that Gene” using the handouts from class.
 Read B23-B26 (57-60)
 Read B35-B40 (69-74)
 Read B51- B55. (85-89)Transforming Bacteria with Recombinant Plasmids.
o Answer the “What do you already know?” ?’s in your lab book
 Read B56-B63. (86-97)
o Copy the Laboratory 5: Transforming Bacteria with the ligation products
materials and methods into your lab book. Fill out the BACTERIAL
GROWTH PREDICTIONS worksheet from class.
Bring with you to class on Monday.
 Answers to questions on pages:
o A30 (32) #1-4
o B3 (37) #1-3
o B17 (51) #1-4
o B30 (64) #1-3
o B35 (69) #1-2
o B61 (95) #1-4
o B64 (98) #1-7
 Either photocopy or send via email ( naberm@svusd.org ) a copy of your
stamped pages.
 Clone that gene activity sheet (CLONE THAT GENE: PLASMID DIAGRAM) and the
answers to the questions on page B11 (45) #1-3.
 Your completed BACTERIAL GROWTH PREDICTIONS worksheet.