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论文写作
陈如平 2013.8.13

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Title
Abstract
Introduction
M&M
Results
Discussion
References www.xianimi.com
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1. Give the species of the organism, or the cell lines you are studying.
-mice ： 品系 ， 数目，性别，体重，周龄，来源及饲养条件；
( approved by the Institutional Animal Care and Use Committee of…).
Twenty 12-week old female BALB/c mice were purchased from
Jackson Laboratories. Mice were raised under standard conditions of…and fed on …with access to free access to water.
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- Cell line: 来源，培养基及培养条件
HCT116 cells (a gift of B. Vogelstein) were grown in McCoy's medium plus 10% FBS, P/S. All cells were maintained at 37
°
C in
5% CO
2
.
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2. Describe the source of chemicals and reagents.
-from a company, give the name and location of the company. 平时整理一份常用试剂的公司
-gifted by some person, give the name, institute and location.
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3. Give details such as temperature, pH, amount of reagent and sampling times.
4. Use a method that is common in your field, you can reference the paper that describes this method.
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5. Use scientific and specific language.
Don’t use vague or imprecise words
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实验方法的描述就是一系列专业科学动词的运用 cultivate; culture; decant; destain; determine; develop; dialyze; digest; disperse; dissolve; divide; elute; express; extract; filter; freeze; fuse; grow; hybridize; immobilize; immunoprecipitate; incorporate; incubate; inject; inoculate; resuspend; separate; stain; transfer; transfect and etc.
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常用方法总结
Western blot; qRT-PCR; MTT; BrdU; Tunel staining; ELISA;
Immunocytochemistry; Immunohistochemistry;
Scan flow cytometer; RNAi; Overexpression etc.
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结果的表现形式
Table Graph Figure
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To tell a story: in step by step style
Initial hypothesis
Experiments to test
Supported or not
Second question
/hypothesis www.xianimi.com
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首先整理出一套清晰的思路，按照思路来作图。
写一个以 subheading
为主的框架，然后在具体写
作。
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Begin with
To/in order to find/determine/discover/ measure/study/analyze/test/better understand …
-To determine whether the defect affects transcription of the gene, we isolated RNA from the cells and quantified the RNA by RT-PCR.
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Results description
The results/data showed/exhibited/demonstrated/…, or we found/… that….reduced/increased/decreased/up or down-regulated….(Fig or Table).
-qualitative description and quantitative description
-significant statistics （ variable over time ； comparison among groups ； relationship between variables ）
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Figure legend
-To explain all the symbols and different groups shown in the figure.
-Brief description of the experiment.
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Significance
Explain what is new in your work and why this matters to research focus.
-To explain the importance of the discoveries
-To spread the implications of the study www.xianimi.com
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Begin with
-The main findings of this study…
/In the present study, we demonstrate/describe/find/…
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根据结果内容，总结 3-4 个点，分别进行讨论。
-Whether get the expected results or not ， and explain.
-Compare with other studies: in line with or not, and give the possible reason.
-Present an idea and list related studies to support.
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End with
-limitation
-implication
-need for more research www.xianimi.com
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1. Background
2. Purpose
3. Methods
4. Results
5. Conclusions www.xianimi.com
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Background
Briefly describe the background and context to the study.
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Purpose
-Identify the particular research problem, objectives or hypotheses.
-Sometimes, point to a need for research/more research. www.xianimi.com
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Methods
-what you did or measured, and how you did it.
Results
-List important data specifically and briefly.
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Conclusions
-State the contribution of the work to the area of research and its implications.
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