Supplemental File S5. Cell Engineer-PowerPoint

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Pre-activity Assignment and Assessment

Activity:

Cell Engineers and Cell Detectives

Post-activity Assessment

– Assessment

Ask “ detectives ” to guess the function of the mystery cell, and explain why.

• Ask “ engineers ” to respond.

• Encourage discussions and clear up any misconceptions

– Mini lecture

• Mesophyll cells (photosynthesis) vs. storage cells

– Both have (different types of) plastids

Goblet cells vs. neuronal cells

– Both have extensive ER/Golgi (one for secretion, one for making membrane)

– Clicker activities

Guess the functions of a series of cell types with specific organelle contents and structural features

Conclusions and Perspectives

– Linking micro (organelles and cell function) and marco (organism level)

– Inspire students to think about bigger questions

• How are cells different (differentiation)

• How does one cell (zygote) make different cell types following a spatial and temporal program (development)

How have all these cell types come into being (evolution)

What does a photosynthetic plant cell look like?

http://www.uri.edu/cels/bio/plant_an atomy/images/01.gif

https://faculty.unlv.edu/schulte/Anatomy/Leaves/PrivetLeaf.jpg

– Individual cells: high chloroplast content and other features

– Working together: difference between two major types of mesophyll cells

What do these cells do?

Avocado

Potato http://www.emc.maricopa.edu/faculty/farabee/biobk/amyloplast.jpg

http://nickrentlab.siu.edu/PLB400/Peterson_Images/

Persea_Lipid.jpg

– They also contain numerous plastids

• Same type of organelle as chloroplasts

– What do they do?

What does a goblet cell look like?

http://www.baileybio.com/plogger/images/anatomy___physiology/12.

_powerpoint_-_digestive_system/goblet_cell.jpg

http://www.bio.davidson.edu/people/kabernd/BerndCV/Lab/Ep ithelialInfoWeb/goblet cells .jpg

– High abundance of ER and Golgi

– A trans Golgi apparatus cisterna is converted to mucinogen granules every 3 to 4 minutes during active secretion

What do these cells do?

http://www.columbia.edu/itc/hs/medical/sbpm_histology_old/micrographs/15.jpg

http://cdn.head-fi.org/b/b0/200x200px-ZCb0c4ee5f_ChkNFH-neuron1.jpg

– High abundance of ER and Golgi

– What do they do?

Clicker Time!!

What do these cells do?

Fiber cell in Papyrus Stone cells in pears http://emp.byui.edu/wellerg/Cell Types and

Tissues Lab/Images/Fiber.jpg

http://emp.byui.edu/wellerg/Cell Types and

Tissues Lab/Images/Sclerids.jpg

Structural features:

dead; very thick secondary cell wall

Function: a) Decoration?

b) Support / protection?

c) Enlarging surface area?

d) Water preservation?

e) Other?

What do these cells do?

http://www.cas.muohio.edu/~meicenrd/anatomy/Ch

7_Protective/pcep.jpg

http://www.unimuenster.de/GeoPalaeontologie/Palaeo/Palbot/c ut1a.jpg

Structural features:

Tightly packed together; thick waterproof cuticle layer

Function: a) Decoration?

b) Support / protection?

c) Enlarging surface area?

d) Water preservation?

e) Other?

What do these cells do?

http://upload.wikimedia.org/wikipedia/c ommons/7/75/Arabidopsis-epidermand-trichome.jpg

http://www.queensu.ca/biology/new s/sneddentrichome/trichome.gif

http://t1.gstatic.com/images?q=tbn:ANd9G cRrA2tze02y1uajzmDCObWYYXfhnL4Cu

FIe72KwKcEINNGW47wq

Structural features:

sharp outgrowth

Function: a) Decoration?

b) Support / protection?

c) Enlarging surface area?

d) Water preservation?

e) Other?

http://www.namethatplant.net/Images/Ima gesTN/tn_mam/tn_mam_roughleafdog06

0310dz.jpg

What do these cells do?

http://botit.botany.wisc.edu/Resources/Botany/

Root/Grass Root Tip/root hair.jpg

http://www.bio.miami.edu/d ana/pix/roothairs.jpg

Structural features:

more delicate outgrowth

Function: a) Decoration?

b) Support / protection?

c) Enlarging surface area?

d) Water preservation?

e) Other?

http://www.amjbot.org/co ntent/90/6/823/F3.large.j

pg

What do these cells do?

http://lh5.ggpht.com/_wYv4UjyptOQ/TPMRfco0LpI/AAAA

AAAAAzA/uJMDI2XWDr0/image131_thumb.png?imgmax

=800 http://discoverysedge.mayo.edu/diabetesmetabolism/images/de10-4-diabetes-myofibril.jpg

Structural features:

numerous mitochondria & myofibrils

Function: a) Decoration?

b) Support / protection?

c) Enlarging surface area?

d) Water preservation?

e) Other?

Summary

• What have you learned today?

– Relationship between organelle content, cell structure and cellular functions

• What new questions do you have now?

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