The appearance of inconsistent expression within four of our small molecule probes
suggested that there could be issues with their performance. The probes, Ornithine,
Arginine, Proline, and Leucine, were treated to a new method of purification and
validated. Staining was repeated and the pattern remained. When the data was
unmasked and compared by presumed groups the inconsistency fit within those
groupings, some with statistical significance. It is possible that batch processing and
inconsistencies with staining can explain differences, as would harvest times and
fixation periods.
Average Pixel Intensity
160,00
140,00
120,00
100,00
Six3
80,00
Cre i
Ctrl
60,00
40,00
20,00
0,00
TT
O
R
P
L
Taurine (TT), Ornithine (O), Arginine (R), Proline (P), and Leucine (L)
expressions by average pixel intensity of retinal section. Grouped by
presumed distinctions, Six3, Cre I, and Controls. SE.