CHAPTER NO.4: ANIMAL TISSUE GENERAL ZOOLOGY (LECTURE) BS PSYCHOLOGY | Doc. Annie Gallardo | 3rd year – Junior Year I. II. III. IV. OUTLINE Epithelial Tissue Glandular epithelium Germinal epithelium Connective tissues Bone Cartilages Areolar Dense connective tissues Adipose tissues Reticular connective tissue Blood/vascular tissues Hemopoietic/Hematopoietic tissues Muscle tissues Skeletal Cardiac Smooth Nervous tissues Neurons Glial cells EPITHELIAL TISSUE ANIMAL TISSUES Tissues - a group of cells that are similar in structure and function Histology - the study of tissues EPITHELIAL TISSUES Sometimes simply called epithelium Basic type of epithelial tissues based on their shape: Squamous epithelium Cuboidal epithelium Columnar epithelium Cells are completely placed with one another and with very little intercellular or cementing substance between the cells They cover the external surface of the body and the internal surfaces of cavities in the body Epithelial tissues form the covering of all body surfaces Composed of 1 layer of cells They are said to be simple, i.e., the simple squamous, the simple cuboidal, and the simple columnar Stratified epithelium can be found in several layers of cells Epithelial tissues give rise to most glandular structures The major tissues found in the glands are basically epithelial in nature and they also form parts of all the sense organs Special characteristics of epithelial tissues: They form continuous sheets They are provided by a basement membrane They are said to be vascular (not so much provided with blood vessels) They are regenerating The basic function of the epithelial tissues: Protection - covering the outer and inner surfaces of the body Absorption - some of the epithelial tissues are provided with cilia Filtration - found in glands 1|N O MAN I S AN I SLAND Secretion - being in glands, they are glandular in nature and are also germinal in nature (the same epithelial cells covering, e.g., the internal surfaces of the ovary and the testes) Formation of reproductive cells Types of epithelial tissues based on the number of cell layers: Simple Stratified Pseudostratified Transitional Types of epithelium based on the shape of cells: Simple squamous o Cells are sent to be flat and irregular Cuboidal o Cells are seen to be shorter but wider o In the form of cube Columnar o Cells are tall and thinner Simple type of epithelium o cells are one layer only, i.e., simple squamous, simple cuboidal, and simple columnar Stratified type of epithelium o cell layers are two or more than two layers, i.e., stratified squamous, stratified cuboidal, and stratified columnar Types of epithelial tissues based on the number of cell layers: Squamous epithelium o Made up of thin flat cells that resemble the blocks o Also known as mesothelium because they line the silom or body cavities Simple squamous epithelium o Made up of 1 layer of flattened cells o Also known as endothelium and found in the lining of the inner surface of blood vessels, in the ducts of numerous glands, and lining of the membranous labyrinth of the inner ear. Stratified squamous epithelium o Made up of two or more layers of flattened cells o Found lining the nose and mouth cavities, lining of the vagina, the stratum corneum of the epidermis, in the vertebrate skin, and the outer portion of the cornea of the eye Simple columnar epithelium o Made up of cells that are much taller than their width o Seen to have columnar cells adhering to each other along the longitudinal or lateral surface (magkakatabi), and then the nucleus is elongated but commonly basal in position o Mostly found in the tunica mucosa of the gastrointestinal tract; the innermost lining of the alimentary canal, or the digestive tract, from the stomach to the anal regio Stratified columnar epithelium o Made up of several layers of columnar cells o 2|NO Found in the stratum germinativum of the skin; in the innermost layer of the epiglottis, and part of the urethra, as well as faults of the conjunctiva of the eye Simple cuboidal epithelium o The height of the cells is about as tall as their weight, so they look like cubes o Made up of 1 layer of these cuboidal cells o Seen in the lining of ducks, in the glands like the thyroid gland, the kidney's tubules such as the uriniferous tubules Stratified cuboidal epithelium o Made up of several layers of cuboidal cells o Found in the epidermis of many tailed amphibians, specifically the urodeles Ciliated or flagellated epithelium o May be columnar or cuboidal cells, which are provided with cilia or flagellum o The columnar ciliated epithelium are columnar cells with cilia o Found at the innermost lining of the trachea of the terrestrial animals, e.g., in the dog and they are also found in the intestine of earthworms Cuboidal ciliated epithelium o Cuboidal and yet they are provided with cilia o Found in the sperm ducts of earthworms and oviducts of the frog Flagellated epithelium o Provided with flagella o Found at the inner layer of the hydra and the regional canals of sponges Pseudostratified epithelium o The tissues are formed by a single layer of cells but hey are of different heights o Gives the appearance of being stratified, but in reality they are only made up of a single layer of the cells and that the nuclei are of different heights o Generally, the cells are elongated and their nuclei are more densely packed within the tissue o Found in the respiratory tract and inner ear of the mammals, in the epididymis, in the prostate gland, and also in the vas deferens Pseudostratified ciliated columnar epithelium o Found along the respiratory tract o Help trap and transport particles brought in through the nasal passages, and these are through among the mammals Transitional epithelium o Also known as eutrothilium o Made up of multiple layers of cells that become flattened when stretched o It lines most of the animals urinary tract, such as the urethra, the ureter and the urinary bladder, and it allows specially or specifically the cells of the urinary bladder to expand when it is filled with urine and the cells will go back to its natural shape, which is typically squamous when it is distended, meaning when the urine is removed or excreted out. So it can go back to its natural shape when distended, and this is in its columnar shape (referring to the diagram) o Specialized to change in shape in response to increased tension MAN I S AN I SLAND o Called transitional cells because they represent a transition between two desperate epithelial cell types, so from squamous to a columnar cell type (in relation to the previous bullet) Type of epithelium based on function Glandular epithelium o Specialized for secretion of substances or materials o Also called the exocrine glands because these glands are provided with tubules for the passage of their secretion o (REFER TO THE DIAGRAM) So if you're going to look at the diagram of a gland, this is how the glandular epithelium looks like forming the gland. These are the glandular epithelium and they have a space in between them, forming the dock of the gland, and then we have this glandular epithelium which are actually the secretory cells and the glandular epithelium are again specialized for secretion Types of glands based on the number of cells Unicellular gland o Made up of only one cell, just like in the GID or the gastrointestinal tract where you find the goblet cells o Goblet cells are unicellular glands, they are one celled glands, so these spaces here are the goblet cells (referring to the diagram) Multicellular gland o The rest of the glands are multicellular o Made up of many cells just like what we have in these two diagrams (referring) (REFER TO THE DIAGRAM) o For the multicellular glands, we have tubular glands and we have saccular glands, they look like sacs or alveolar glands or saccular glands. Simple types if they only have one tubule and if they branch out, then we call that as branch tubular; they can be called tubular and still these tubular glands are producing alveolus at the terminal end. We have this branch alveolar which are a more complex form of glands found in mammals, which are the compound tubular acinar and we have the compound acinar. Multicellular glands Simple tubular glands o Short blind tubes o Found on the tampads of male salientants (what?) in the mental glands of some salamanders Simple coiled tubular glands o Provided with a long narrow tube and the distal end of which is coiled into a small ball o Found in the sweat glands in the skin of mammals Simple branched tubular glands o A simple tube which divides its distal end into two or more branches. There are terminal portions which may or may not be coiled. These are found in the large sweat glands of the axilla or the armpits. Compound tubular glands o The compound tubular glands are with varying numbers of tubules. o Ex: Mammary glands of the monotremes. Simple saccular glands o The simple saccular glands are provided with one expanded bulb at the end of the duct. These are found in the mucus and poison glands in the skin of amphibians. Simple branch saccular glands o Provided with several acini arranged along a single excretory duct, like in the meibomian glands of the eyelid. These are found in reptiles or a single acini appears to be divided by partitions into several smaller acini, as in that is found in the sebaceous or oil glands of the skin. Compound saccular glands o Consists of several portions called lobules, corresponding to the simple saccular glands. These several units are structures entering a common duct. o Ex: In mammary glands of the metatherians and the Eutherians. Germinal Epithelium From the word germ, referring to the germ cells, these epithelial cells lining the walls of the testis and the ovary will later on give rise to the sperm cell and the egg cell. Found in the lining of the seminiferous tubules of the testes. Germinal epithelium diagram: o Known as the wall of the seminiferous tubules. These cells are connected via the tight junction. o These are the same cells which are the germinal epithelium and will later on become the spermatogonium, the primary spermatocyte, the secondary Spermatocyte will grow into the spermatim, and later on to become the spermatozoa. o This is also the cells lining the ovary. So, the epithelium is found along the wall of the ovaries or the ovarian surface epithelium. o This is also called the germinal epithelium of the Waldeyer o This is a layer of simple squamous epithelium. o From a simple squamous to cuboidal epithelial cells covering the ovary, and this same covering of the ovary will later on become the oogonium, the primary oogonia, oocyte, the secondary oocyte, the ootid, and will later on become the egg cell. CONNECTIVE TISSUE 2nd type of animal tissue is the connective tissues. Common characteristics of the connective tissues: 1. Cells are loosely arranged; seem to be far apart from each other. 2. They are embedded on a matrix and the matrix contains a large amount of intercellular substance or cementing substance between the cells. This matrix could either be a solid matrix or a liquid or fluid matrix. 3. The cells are usually enclosed within an empty space called lacuna, such as in the 3|NO MAN I S AN I SLAND bone and in the cartilage type of connective tissue. This matrix also is provided with fibers, they are called based on the protein component of these fibers – Collagenous fibers: Contains collagen; Elastin fibers: contains elastin protein. 4. They are well vascularized – they are provided with many blood vessels except for tendons, for ligaments and cartilages. 5. There is an abundant intercellular matrix. 6. They show a very slow regeneration compared to our epithelial cells. Type of connective tissue/substantive tissues Elastic Connective Tissues Dense Connective Tissues Loose Connective Tissues o Adipose o Cartilage o Bone or osseous tissues Blood/Hemopoietic tissues Main functions of connective tissues 1. Binds structures together. 2. Forms framework and support. 3. Stores fat. 4. Transport substances. 5. Protects against diseases. Specialized Types of Tissues Bone/Osseous Tissues o Made up of cells called bone cells or osteocytes, located in a space called lacunae, lacuna in plural form. o Embedded on a hard matrix. o Lacuna are arranged around a cavity called Harvesian Canal and this communicates with one another through canaliculi o The unit structure of the bone is the Haversian system, and it is composed of all the Haversian canals and all the lacunae with bone cells surrounding it. o These bone cells are found in the bones of animals. o Main function of the bone is for support. Hence, it is known as the framework of the body. o Periosteum The cross and transverse section of a bone showing the outer covering. o Two types of tissues found in the bone: Compact Tissue where muscles are attached via the tendons, and which ultimately provide the structural and locomotor support to the body. It also functions to protect the vital organs of the body as it is the layer of tissue above the important flat bones, such as the ribs that protects the thoracic cavity where you find inside the lungs and the heart, the respiratory and the cardiovascular organs. The skull or the brain case that protects the brain and the vertebral column that encloses and protects the spinal cord. The compact bone tissues are found under the periosteum and in the shaft or diaphysis of a long bone Example of a long bone is the femur and the surface of flat bones, such as the cranium. 4|NO Spongy Bone Tissue Shock absorber during periods of movement, such as in jumping, in, walking, and running. Handles the more active functions of the bone, which is the hemopoiesis, or the production of blood cells as well as in ion exchange. Found in joints as a shock absorber in the ends of long bones, which we call epiphysis, and in the interior of flat, short and ankle bones, and irregular bones such as the maxilla. Cartilage o The cartilages have the intercellular substance called the matrix, which is sometimes calcified. o It is impregnated with inorganic substances like calcium and the membrane, covering a cartilage is called perichondrium. o There are three types of cartilages found in the adult body of animals and in the human body: Hyaline Cartilage Most abundant type of cartilage. Found at the of the ribs, at the ends of long bones, in the nose, parts of larynx, the trachea, the bronchi, the bronchial tubes, in the embryonic and fetal skeleton. Also found in the endoskeleton of adult sharks and rays. Has no fiber in it. Weakest type of cartilage. Can be fractured while performing its function of providing flexibility, support and providing smooth surfaces for movements at joints. Elastic Cartilage Found in the auditory tubes, in the part of the external ears or Oracle in animals, this is found in the external ear called the pinna of the ear, in the epiglottis or the lid on top of the larynx Can also be found in the cartilage of the eustachian tube. Contains elastic fibers that are branch. Maintains shape of certain structures. Provides structural strength and elasticity. Fibrocartilage The intercellular substance contains the collagenous fibers that are not branch. It is found in the intervertebral disc that is in between vertebrae, in the pubic symphysis or in joints which are subject to severe strain. It is also found in the cartilage pads of the knee called menisci and portions of tendons that are inserted into the cartilage. Rigid but is the strongest type of cartilage and is reliable in supporting and joining structures together. MAN I S AN I SLAND Fibrous Connective Tissues The next type of connective tissues are the fibrous connective tissues, and under this we have the loose connective or the areolar connective tissues Areolar connective tissues o The fibroblast are oval cells or round supported by a matrix with the collagenous and elastic fibers. o These are found in the subcutaneous tissues in the papillary dermis, in the lamina propria of the mucous membrane around the nerves, the blood vessels and body organs they are also found in the mesenteries and omenta. o This is how a loose connective or areolar connective tissue is seen. o You find here the thick, collagenous fiber, and thinner, but branched elastic fibers and you find here the oval or round fibroblast cells. Dense connective tissues o now the dense term here is referring to the collagen fibers that are found in the matrix of the connective tissue. o The cells are also called fibroblasts and they are found at random embedded on the fibers. o There are fibers that are seen to be compactly arranged, and we have two types of these arrangements: Regularly arranged dense connective tissues The regularly arranged connective tissues forms the tendons which attaches the muscle to the bone. Most ligaments, which attaches bone to bone. And aponeurosis which are sheet like tendons that attaches the muscle to muscle or muscle to the bone. Irregularly arranged dense connective tissues. They often occur in sheets, such as in fascia – tissue beneath the skin and around muscles, and other organs, in the dermis of the skin, in the fibrous pericardium of the heart, the periosteum of the bones, perichondrium of cartilage, in the articular cartilage or capsules, the capsules around various organs, such as surrounding the liver, kidney, testes, lymph nodes, and heart valves. Adipose Tissues Found under the Group of Areolar Tissues because they have some scattered fibers, which are loosely arranged. The adipose tissues are made up of cells which are called fat cells or adipocytes. The cells are rounded with thin walls and very little cytoplasm. The nucleus is situated on one side of the cytoplasm. Because the cytoplasmic area is almost occupied by fat cells. These are used to store fats so the main function of adipose tissues is to reduce heat loss They are also energy reserves they support and protect organs, and they generate heat. Examples of these are found in the subcutaneous tissues around the heart and kidneys, yellow bone marrow, the padding around the joints. they are also found behind eyeballs in eye sockets and very good example of these are also the fat bodies, or the corpora adipose in the frog. The microscopic view of the adipose tissues where you find the cytoplasmic areas almost empty because these are reserved issues, but you can find a very thin cell membrane and you will find the darkly-stained structures there which are the nucleus found at the periphery of the cells. Reticular connective tissues A diagram showing a cross section of a lymph node, which is an example of a reticular connective tissues. In this type of tissue, the cells are called fibroblast and they are provided with processes and all of these processes in the fibroblasts are forming a network, hence the term reticular. The fibroblasts are supported by a matrix with reticular fibers. This connective tissue supports the blood cells in lymphoid organs. They form the stroma of organs. They bind smooth muscle tissue cells. Fillers, and remove worn out blood cells in the spleen and microbes in lymph nodes. The reticular connective tissue is found in the stroma of the liver in the spleen, in the lymph node, in the red bone marrow, the reticular lamina of the basement membrane around the blood vessels and muscles. Blood Tissues or Vascular Tissues The blood is made up of specialized connective tissue, which transports substances. The cells are called blood cells or corpuscles, and these are supported by a fluid matrix called the plasma. The blood vascular tissues are the vehicle for the cardiovascular system. o It is composed of the plasma, which is the fluid part which functions to transport substances and constitutes 55% of the blood, and it is also made up of the formed elements or cells which constitute 45% of blood cells. o RBC or red blood cells or the red blood corpuscles this is also called erythrocytes and they are specialized for transport of gases. o WBC, the white blood cells, or white blood corpuscles what we call the leucocytes, and they are considered as the soldier cells of the body. o Platelets the platelets are the cells which are involved in blood clotting or blood coagulation. We have another diagram here, so showing the RBC, the WBC in different forms, and platelets, then together with the blood vascular tissues, we find also the hemopoietic or hematopoietic tissues 5|NO MAN I S AN I SLAND These are tissues that are specially involved in the formation and maturation of blood cells The type of hemopoietic tissues are the lymphoid tissues which form the lymphocytes and the monocytes, and they are found in the lymph nodes in the liver, in the spleen. o Myeloid tissues. They form the erythrocytes and the granulocytes and they are found in the bone marrow The following are the different types of WBC or white blood cells. o The agranulocytes or the mononuclear leukocytes Between the two, we have monocytes and lymphocytes. The darkly stained here is the monocyte and this darkly stained structure here is the lymphocyte. The monocytes are bigger spherical cells and the nucleus inside is bean shaped. It has no indentation, and we say that 2 to 6% of the total WBC are the monocytes. o Lymphocytes. The lymphocytes, compared to the monocytes, are smaller spherical cells, and their nucleus are almost occupying the entire cell. The cytoplasm is very small, in amount: 20 to 25% of the WBC are the lymphocytes. o Granulocytes because they have granules in the cytoplasm and their nucleus are seen to be varying in shape. They are also called the polymorphonuclear leukocytes. Basophil. The basophil has one low nucleus which is twisted like an “S”. There are granules in the cytoplasm and are the granules that are very large, but few. They can be stained by basic dyes. And 0.5% of the total number of WBC's are the basophils. Eosinophil. The eosinophil is made up of nucleus, consisting of two lobes, and the granules in the cytoplasm are fewer but coarser, and they can be stained by the acidic dyes during the preparation of WBC 3 to 4% of the total number of WBC are the eosinophils. Neutrophils whose nucleus consist of three or more lobes. The granules in the cytoplasm are fine and numerous They are not stained by acidic nor by basic dyes, and we say that 60 to 70% of the total number of WBCs are the neutrophils. MUSCLE TISSUE The muscular tissues or muscle tissues, and these tissues are specialized for contraction, the cells have the ability to contract, and these cells are called muscle cells or muscle fibers, and they are enclosed by a sarcolemma. Myofibrils This is the contractile fibrils contains of the sarcoplasm of the cell and muscle cell contraction involves the interaction between filaments of myosin and actin. Muscle tissues are highly masked cellular, and they are well supplied with blood vessels. That's why when you cut the muscle, it bleeds a lot. The muscle cells or muscle fibers are long and slender, and they are basically made-up of protein called actin and myosin. Most of the muscle tissues are voluntary and under conscious control and some are involuntary. You know the voluntary are these skeletal muscles that are found attached to the skeleton of animal cells. While the involuntary muscles are those that are found in the heart and found in the internal organs or the smooth muscle cells. In animals, 50 to 60% of the total body weight is made by the muscles. Skeletal muscle The striated voluntary muscle The cells are filamentous or elongated cells, and they are found to have striations, so they are said to be striated muscles. They are multinucleated cells and as the nervous control, they are voluntary in action. They are the muscles that are found attached to the skeleton or to the bones. Hence, the word skeletal muscles The movement being accomplished by the skeletal muscle is for the movement of the general body movement. Cardiac muscle The striated involuntary muscle The cardiac muscles are also striated. The cells are branching and each of these cells are found to have striations of both the myosin and the actin filaments. There are one to three central nuclei. Cardiac muscles are also mononucleated and then it is involuntary as to nervous control. The cardiac muscles are involved in the contraction of the heart Smooth muscle The smooth involuntary muscle or visceral muscle. They are found not to be striated so they are said to be nonstriated. They are mononucleated, they have a single central nucleus. The cells are slender and tapering at its end, and as to nervous control, it is involuntary in action. The smooth muscles are for the movements of the parts of organs. NERVOUS TISSUE The last type of Animal tissues are the nervous tissues and this tissue is specialized for the reception of stimuli and transmission of impulses. Majority of the neural tissue in the body of the animal is concentrated in the brain and the spinal cord, which comprises the central nervous tissues. Neurons The first type are the neurons or the nerve cells. So the nervous tissues are made-up of cells called neurons, and they are the one which transmits signals they carry impulses. 6|NO MAN I S AN I SLAND Soma o The soma or the cell body containing the nucleus. Dendrites o There are protoplasmic processes in the form of the dendrites. o There are more than dendrites and these dendrites transmits impulses towards the soma Axon o which transmits impulses from the soma towards the synapse. The synapse is a specialized intercellular junction where the axon ends. The main function of our Nervous tissues is to coordinate and control body activities and the protein that made-up our nervous tissues is the myelin. Basic types of neurons based on the number of cell processes. The unipolar neuron o The unipolar neuron is a type of neuron with one process. o There is only one process this is the soma or the cell body. o It has one process which will branch into an Axon. o The dendrites are beyond the perikaryon and then we find this type of unipolar neuron from the dorsal root ganglion of the spinal cord. o Most of the sensory neurons are unipolar and in animals they are found in earthworms. o The bipolar neuron o The bipolar neuron with one Axon, and one dendrite. o The bipolar are found in the cones and rods. These are in the fovea of the eyes, and it is also found in the nose o The multipolar neuron. o The multipolar neuron with one Axon and several dendrites. o We can find the multipolar neuron from the grey matter of the ventral horn of the spinal cord. o The multipolar, all the motor and interneurons are multipolar type of neurons, and these are again found in the roots of the spinal nerves. o Most of the multipolar shapes are basically for thinking, memory and decision making in the human body. Types of neurons, based on the action, the sensory type, the motor, and the associative interneuron or integrative type of neurons. The unipolar are mostly the sensory neurons, o The sensory neurons carry impulses from the receptors to the spinal cord. The bipolar are the interneurons o Interneurons are located entirely within the central nervous system, and these are 99.8% of the central nervous system. The multipolar are the motor neurons o The motor neurons carry impulses from the brain to the effectors while the relaying neurons or Interneurons carry impulses to and from the spinal cord and the brain. The Schwann cells wrapped around an Axon. They are the glial cells that surround the neuron, and they myelinate the axons or cover them with a myelin sheath. The Schwann cells are the major glial cells found in the peripheral nervous system and they play essential roles in the development, in the maintenance, in the function and regeneration of peripheral nerves. So as a whole these Schwann cells support the nerves. The other type of nerve cells is the neuroglia or the glial cell. The neuroglia are also all three parts or three types, the oligodendrocytes, the microglia, and astrocytes. So, we say that there are three main types of neuroglial cells that are recognized. Oligodendrocytes or oligodendroglia o They form the myelin sheath that surrounds many neuronal axons, and these increase the rate of conduction. Microglia o on the other hand, has a phagocytic role in response to the nervous system's damage. Astrocytes o The ones that provide biochemical support for endothelial cells that form the blood brain barrier, so these are supporting cells of the neurons or the nervous system. 7|NO MAN I S AN I SLAND