Research Question How does the immediate cessation of physical activity affect the strength and body composition of physically active humans in a time window of 20 days? 1. Personal Engagement In my family environment, as everyone does sports and is very physically active, I couldn’t be any different. Since I was 8 years old, I have been completely addicted to sports, nutrition, and going to the gym. As I have always done sports, I have made many friends with the same interests and goals as me. Mostly all of them are in good body shape, eat healthy food, and love sports. As a 16-year-old Brazilian, I consider myself and my friends kind of different from the rest of the people our age, while they are going out a lot and consuming alcohol we are taking care of our health. In Brazil, there is a famous holiday named Carnival, which lasts 10 days. Me, my friends, and my family decided to go on a trip. We rented a house in the countryside and stayed there for 15 days chilling and resting. We wouldn't change our lifestyle of eating healthy food and having a great time of sleep, however, there is no gym or a place to do sports. Therefore, I start inquiring about what will happen to our bodies if we just stop working out. 2. Background information WHO defines physical activity as any bodily movement produced by skeletal muscles that requires energy expenditure, such as taking a walk or playing a football match. There are several intensities of physical activity, ranging from light to vigorous. Incorporating different intensities can target different muscle fibers, with endurance activities primarily engaging slow-twitch fibers and high-intensity activities targeting fast-twitch fibers. Any intensity up to moderate can improve the health of individuals. Popular forms of physical activity include working out in the gym (lifting weights), cycling, running, and participating in sports. Engaging in regular physical activity can reduce the risk of heart disease, stroke, diabetes, and several cancers. It also aids in preventing hypertension, maintaining a healthy body weight, and can enhance mental health, quality of life, and overall well-being. Exercise triggers numerous positive biological responses in the human body. A key change is the increase in metabolic rate. Regular physical activity boosts the body's metabolism, meaning it burns more calories at rest. This elevated metabolic rate can persist even after the exercise session is over, a phenomenon known as the afterburn effect or excess post-exercise oxygen consumption (EPOC). Exercise also enhances muscle fat utilization, strengthens ligaments and tendons, and increases both lactate threshold and capillary density in muscles. Additionally, VO2 max, a measure that indicates the maximum oxygen use during intense workouts, also rises. Over time, these adaptations make the body stronger and more efficient, enhancing athletic performance. The more consistent an individual is with their exercises, the quicker these benefits manifest, leading to a faster progression in strength and health. Working out at a gym exemplifies physical activity that triggers these biological reactions. This exercise form, particularly resistance training, emphasizes lifting heavier weights with fewer repetitions, a method known as low-volume, high-intensity training. This type of training predominantly engages fast-twitch muscle fibers, leading to rapid gains in strength and muscle size. The primary goal of such training is to enhance physical strength. Consistent resistance training over time can result in increased muscle mass (hypertrophy), stronger bones, reduced body fat, and improved mental well-being. Detraining, as defined by the National Institute of Health, refers to the physiological and performance declines observed when an individual ceases regular physical activity. While consistent exercise can lead to gains in strength, muscle mass, and neuromuscular adaptations, these benefits can wane during periods of inactivity. Additionally, detraining can lead to a decrease in the metabolic rate, meaning the body will burn fewer calories at rest. This reduction can contribute to weight gain and a decrease in overall fitness. The magnitude of these changes is influenced by several factors, including the length of inactivity, an individual's genetics, diet, sleep quality, and more. The concept of detraining underscores the "use it or lose it" principle, highlighting the significance of ongoing physical activity to preserve fitness achievements. 3. Hypothesis In physically active individuals, the cessation of all forms of physical activity can lead to a decline in muscle strength due to the muscles not being actively engaged. This is supported by a study titled "Substituting sedentary time with physical activity in youngest-old to oldest-old community-dwelling older adults: Associations with body composition", which found that replacing sedentary behavior with moderate-to-vigorous physical activity was associated with a decrease in body fat percentage, BMI, and waist circumference. These changes, characterized by an increase in body fat percentage, can occur as caloric expenditure decreases while caloric intake may remain constant or even increase due to sedentary behavior. The research emphasized the strong and independent relationship between physical activity, sedentary behavior, and body composition. Furthermore, the importance of reducing sedentary time is highlighted by the latest WHO guidelines, which suggest that even replacing sedentary time with light physical activity offers health benefits. Such shifts in body composition and muscle strength are expected to be evident within a 20-day period, especially in older adults. 4. Variables Dependent Variables: Measures of body composition including Weight, Body fat percent, Skeletal Muscle Mass, Body Fat Mass, and intracellular water. Plus, a measure of the strength of individuals Independent Variable: Immediate cessation of physical activity Controlled Variables: n 1 Controlled Variable Diet/Nutritional Intake and Hydration Levels Influence on Research Method of Control Ensures that nutrition and hydration do not skew results. Standardized meal plans for all participants, targeting neutral calorie intake and WHO-recommended water intake. 2 Sleep Patterns Medication/Supplement 3 Intake Sleep can affect physical and mental performance. Fixed bedtime for all participants. Certain medications/supplements can influence results. Documentation of all medications and supplements at the start. Exclusion of participants based on certain medications if necessary. Physical activity can affect baseline measurements. Only physically active people will be selected for the experiment. 4 Previous Physical Activity Levels 5 Age 6 Gender Biological differences between genders can influence results. Separate analyses for men and women. 7 Health Conditions Certain health conditions can skew results. Exclusion of participants with health conditions that will affect the experiment. 8 Alcohol Consumption Alcohol can affect physical and mental performance. Participants will affirm non-consumption of alcohol 1 weeks before, during, and 1 week after the experiment via a poll. 9 Environmental Factors External environmental factors can influence results. The study will be conducted in a controlled environment. 10 Measurement Techniques Inconsistent measurement techniques can lead to inaccurate results. Use of standardized equipment and protocols for all measurements. 11 Injury or Illness Injury or illness can affect a participant's performance and skew results. Exclusion of participants who experience injury or illness during the study. 12 Cultural Practices Cultural or religious practices can influence behavior and responses during the experiment. Documentation of any cultural or religious practices that might affect the study. 13 Smoking Smoking can influence physical performance and health metrics. Exclusion of participants with smoking habits. Selection of participants according to Age can influence physical and various age ranges. Different analyses for mental capabilities. each age range. 14 Caffeine Intake Caffeine can affect physical and mental performance. Standardization of caffeine intake among participants as zero. 15 Baseline Body Composition Different body compositions can influence physical performance and health metrics. Measurement and documentation of the baseline body composition for all participants. 16 Training History Past training can influence current physical capabilities. Documentation of the duration and intensity of participants' past training. 17 Adherence to the Study Protocol Non-adherence can lead to inconsistent or inaccurate results. Regular check-ins with participants and use of tools like activity trackers to ensure adherence. High-intensity activities outside of the study can influence results. Ensuring no activity with an intensity higher than low occurs during the study period. 18 Daily Activity Patterns 5. Materials and Apparatus 1. Bioimpedance InBody 270 6. Procedure and Methodology 1. Participant Recruitment: Advertise the research study to potential participants, specifying the age and gender criteria. 2. Participant Selection: From the pool of applicants, select at least 6 participants ensuring the following distribution: 25% of all participants have between 14-25 years. 25% of all participants have between 25-35 years. 25% of all participants have between 35-45 years. 25% of all participants have between 45-60 years. Ensure that out of the selected participants, three (50%) are men and three (50%) are women. 3. Initial Documentation: Conduct an initial interview with each participant to document: Medication and supplement intake. Cultural and religious practices. Training history. Baseline body composition. Provide participants with a poll to confirm non-consumption of alcohol during the experiment and 1 week before and after. 4. Dietary and Hydration Control: Provide participants with standardized meal plans. Ensure participants follow the WHO-recommended water intake (𝑊𝑒𝑖𝑔ℎ𝑡 × 35𝑚𝑙) Monitor and document participants' adherence to the meal plans. 5. Sleep Pattern Regulation: Instruct participants on the fixed bedtime. 6. Environmental Control: Prepare the controlled environment for the study, ensuring consistent conditions for all participants. Monitor environmental factors like temperature, humidity, and lighting. 7. Activity and Exercise Control: Instruct participants on the allowed daily activity patterns, ensuring no moderate to high-intensity activities. 8. Medication and Supplement Regulation: Review the documented medications and supplements of participants. Group or exclude participants, if necessary, based on their medication intake. 9. Data Collection: Use standardized equipment and protocols to collect data on participants' performance, health metrics, and other relevant variables. Ensure consistent measurement techniques across all participants. 10. Monitoring Adherence: Conduct regular check-ins with participants to ensure they are following the study protocol. 11. Handling of Injuries or Illnesses: Document any injuries or illnesses that occur during the study. Exclude participants who experience significant injury or illness. 7. Risk Assessments 1. Safety: Physical Safety of Participants: Potential injuries or adverse reactions due to dietary changes, sleep pattern regulations, or any other interventions. Risk of participants experiencing negative side effects from stopping medications or supplements. Potential harm from wearing sleep or activity trackers continuously. Data Safety: Risks associated with the storage and handling of participants' personal and health data. Potential breaches of confidentiality or data leaks. Equipment Safety: Malfunction or misuse of standardized equipment used for measurements. Risks associated with the controlled environment setup, such as poor ventilation or lighting issues. 2. Ethical Issues: Informed Consent: Ensuring that all participants are fully informed about the research's purpose, procedures, potential risks, and benefits. Ensuring participants understand their right to withdraw from the study at any time without penalty. Privacy and Confidentiality: Ensuring that participants' identities and data are kept confidential and not disclosed without consent. Fair Treatment: Ensuring that all participants are treated fairly and without bias, regardless of age, gender, health status, or any other factor. Handling of Personal Beliefs and Practices: Respecting and accommodating participants' cultural or religious practices that might affect the study, ensuring no participant feels marginalized or disrespected. 3. Environmental Issues: Waste Management: Proper disposal of any waste generated during the study, especially if any biological samples are taken. Energy Consumption: The environmental impact of running a controlled environment continuously, including energy consumption for lighting, temperature control, etc. Resource Usage: The environmental footprint of producing standardized meal plans for participants, especially if non-local or non-seasonal ingredients are used. Electronic Waste: Proper disposal or recycling of any electronic devices, such as sleep or activity trackers, if they are to be discarded post-study. 8. Raw data BEFORE THE EXPERIMENT Male participant 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Female participant 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Skeletal Body Fat Intracellular Weight Body Fat Muscle Mass Water (%) Mass (%) (kg) (kg) (kg) 68.5 7.88 57.3 10.5 75.0 65.3 10.5 63.1 12.8 82.0 70.2 11.0 55.2 14.2 77.5 68.8 8.63 59.7 11.9 72.5 11.3 63.8 13.4 84.5 67.0 71.5 5.73 55.5 8.3 69.0 69.0 9.24 60.8 12.0 77.0 66.7 10.8 61.6 13.5 80.0 69.3 9.54 63.0 12.0 79.5 67.8 9.13 59.5 11.7 78.0 68.1 11.0 61.8 13.2 83.5 68.0 11.2 61.2 13.8 81.0 70.8 7.27 56.0 9.5 76.5 70.0 8.14 59.5 11.0 74.0 1RM Bench Press (kg) 91.25 100.0 102.0 86.25 108.75 85.0 93.75 101.25 103.0 101.0 106.25 103.0 98.0 96.25 Skeletal Body Fat Intracellular Weight Body Fat Muscle Mass Water (%) Mass (%) (kg) (kg) (kg) 71.2 5.81 35.5 10.2 57.0 68.0 6.88 39.0 11.1 62.0 7.62 42.0 12.0 63.5 67.5 70.8 5.93 36.8 10.5 56.5 72.2 5.50 34.8 10.1 54.5 69.2 6.82 38.1 11.0 62.0 5.87 36.8 10.3 57.0 70.7 69.0 6.96 38.8 11.8 59.0 6.57 39.5 10.6 62.0 70.0 5.99 36.8 10.5 57.0 70.8 67.0 7.94 41.3 12.5 63.5 71.5 5.65 37.0 10.0 56.5 67.8 8.00 42.2 12.8 62.5 68.5 7.38 40.2 12.3 60.0 1RM Bench Press (kg) 62.75 68.75 75.00 62.00 51.00 61.00 61.00 66.00 67.00 61.00 75.00 62.00 69.75 67.00 1RM Squats (kg) 1RM Rows (kg) 121.25 116.25 107.0 100.0 110.0 105.0 127.5 116.25 125.0 110.0 143.75 115.0 116.25 108.75 92.5 100.0 95.0 87.5 105.0 85.0 95.0 97.5 100.0 97.5 105.0 97.5 92.5 95.0 1RM Squats (kg) 1RM Rows (kg) 91.25 108.75 98.75 73.75 71.25 88.00 87.50 78.75 92.50 91.25 98.75 73.75 98.75 78.75 71.25 72.5 75.0 68.75 63.75 72.50 70.00 71.25 71.25 70.00 75.00 68.00 76.25 73.75 1RM Biceps Curls (kg) 42.0 46.0 47.5 40.0 47.5 40.0 45.0 46.25 46.25 45.75 48.75 46.25 45.0 43.75 1RM 1RM Lat Pull Triceps Extension Down (kg) s (kg) 87.25 38.25 95.0 41.25 89.25 43.25 82.75 36.25 98.0 42.75 80.5 38.0 88.75 40.0 92.0 42.25 93.0 42.0 92.25 41.25 98.75 44.25 93.0 42.0 90.75 41.0 90.0 40.0 1RM Biceps Curls (kg) 32.75 33.50 35.0 31.50 29.25 33.25 32.00 33.25 32.75 32.00 35.00 31.25 35.00 33.75 1RM 1RM Lat Pull Triceps Extension Down (kg) s (kg) 63.75 29.25 71.25 29.25 72.50 31.00 65.00 28.25 62.50 26.25 71.25 29.25 65.00 28.75 67.50 29.50 71.25 29.75 65.00 28.75 72.50 31.00 65.00 28.25 72.50 31.25 70.00 30.00 1RM 1RM Lat Triceps Pull Extension Down s (kg) (kg) AFTER THE EXPERIMENT New New New New Skeletal New Male Body Fat Weight Body Fat Muscle Intracellular participant Mass (kg) (%) Mass Water (%) (kg) (kg) 75.4 11.3 56.7 8.52 1 68.0 2 82.6 13.2 62.5 10.9 64.5 3 78.0 14.4 54.6 11.2 69.3 4 72.8 12.0 59.1 8.74 68.0 5 84.9 13.7 63.1 11.6 66.2 6 69.3 8.42 54.9 5.84 70.8 7 77.4 12.1 60.2 9.37 68.3 8 80.4 13.8 61.1 11.1 65.9 9 79.8 12.1 62.4 9.66 68.5 10 78.4 11.8 58.9 9.25 67.1 11 83.9 13.3 61.3 11.2 67.5 12 81.3 14.0 60.6 11.4 67.4 13 76.8 9.60 55.4 7.37 70.0 14 74.4 11.1 58.9 8.26 69.3 1RM Bench Press (kg) 1RM Squats (kg) 1RM Rows (kg) 1RM Biceps Curls (kg) 86.69 95.00 96.90 81.93 103.31 80.75 88.06 96.19 97.85 96.05 101.94 97.85 93.10 91.44 115.19 110.44 101.65 94.00 104.50 99.75 119.13 110.44 118.75 104.50 136.56 109.25 110.44 104.06 87.88 95.00 90.25 82.88 99.75 80.75 89.25 92.63 95.00 92.63 99.75 92.63 87.88 92.63 39.90 43.70 45.13 38.00 45.13 38.00 42.75 43.94 43.94 43.46 46.31 43.94 42.75 42.31 Skeletal Body Fat Female Weight Body Fat Muscle Intracellular Mass participant (kg) (%) Mass Water (%) (kg) (kg) 1 57.3 10.6 35.1 6.07 70.5 2 62.3 11.5 38.6 7.16 67.4 3 63.8 12.4 41.6 7.91 66.9 4 56.8 10.9 36.4 6.19 70.1 5 54.8 10.5 34.5 5.75 71.8 6 62.3 11.4 37.7 7.10 68.5 7 57.3 10.7 36.4 6.13 70.0 8 59.3 12.2 38.4 7.23 68.3 9 62.3 11.0 39.1 6.85 69.3 10 57.3 10.9 36.4 6.25 70.1 11 63.8 12.9 40.9 8.23 66.4 12 56.8 10.4 36.6 5.91 71.0 13 62.8 13.2 41.8 8.29 67.3 14 60.3 12.7 39.8 7.66 68.2 1RM Bench Press (kg) 58.75 64.94 71.25 59.38 48.45 58.75 58.75 63.36 65.31 58.75 71.25 62.00 66.75 65.31 1RM Squats (kg) 1RM Rows (kg) 85.94 103.31 94.00 70.88 67.69 83.60 83.13 75.00 92.00 88.44 94.00 73.75 94.00 76.88 67.75 68.75 71.25 63.75 60.56 68.75 66.25 68.75 68.75 68.75 71.25 68.00 76.25 73.75 1RM Biceps Curls (kg) 31.0 31.90 33.25 29.25 27.75 31.25 30.50 32.25 31.0 31.0 33.25 31.25 35.00 33.75 36.34 38.54 41.09 34.44 40.61 36.10 38.00 39.40 39.90 39.09 41.99 39.40 38.85 38.00 78.88 90.25 85.54 78.61 92.90 76.48 84.31 86.80 88.35 87.19 93.44 88.35 85.54 85.00 1RM 1RM Lat Triceps Pull Extension Down s (kg) (kg) 27.75 60.94 27.75 69.00 29.75 72.50 26.25 62.50 25.00 59.38 27.75 69.00 27.38 62.50 28.50 65.25 28.75 69.00 27.75 63.75 29.75 72.50 28.25 65.00 31.25 72.50 30.00 70.00 9. Analysis This section includes the comprehensive compilation of all survey results obtained. Altogether, 31 people participated in the experiment, however, 3 were excluded due to alcohol consumption and heavy medication usage. Of all the 28 participants, 14 are males and 14 are females, happily, this balance between genders enables us to analyze possible differences in the outcome for the different genders. Additionally, an age range was implicated aiming to have a balanced outcome that is reliable for all ages. 9.1. Analysis of the effect of the experiment on changes in strength Before and after the cessation of physical activity, participants did strength tests. The average strength before and after the cessation of physical activity was calculated for each gender at each exercise. Thus, with this means, Graph 1and 2 were created with R. This graph aims to illustrate the difference between the strength in the beginning and at the end of the experiment for both males and females. Graph 1: According to Graph 1, both males and females have experienced a significant decrease in their strength after the immediate cessation of physical activities. While in some exercises people can lift heavier weights, the pattern of decrease in strength seems to be very similar for all exercises even those for both genders. This decrease is expected when someone takes time out from exercise. However, to obtain more precise and reliable outcomes, a paired t-test was proceed. The paired t-tests for various exercises between males revealed statistically significant differences between the before and after measurements, indicating a decline in performance across exercises. For the Bench Press, a t-statistic of 43.188 and a p-value of 1.992 × 10−15 was observed, with a mean difference of 4.906429. This suggests a notable decrease in performance. Similarly, the Squats showed a t-statistic of 24.855 and a p-value of 2.41 × 10−12 , with a mean difference of 5.952857, indicating a decline. The Rows exercise had a t-statistic of 23.069, a p-value of 6.227 × 10−12 , and a mean difference of 4.720714, pointing to a reduced performance. Biceps Curls presented a t-statistic of 32.279, a p-value of 8.485 × 10−14, and a mean difference of 2.195714, suggesting a decrease. The Triceps Extensions had a t-statistic of 26.278, a pvalue of 1.184 × 10−12, and a mean difference of 2.196429, indicating a decline. Lastly, the Lat Pull Down exercise showed a t-statistic of 17.024, a p-value of 2.87 × 10−10, and a mean difference of 4.972143, pointing to a reduced performance. The tstatistic measures the size of the difference relative to the variation in the data. The extremely small p-values in all cases indicate strong evidence against the null hypothesis, suggesting that the observed declines were statistically significant. The mean difference provides the average change in performance, with positive values in this context indicating reductions on the weight lifted by males after the experiment. Similarly, the paired t-tests between females for various exercises also revealed statistically significant differences between the before and after measurements, indicating a decline in performance across exercises. For the Bench Press, a t-statistic of 9.0145 and a p-value of 5.913 × 10−7 was observed, with a mean difference of 2.589286. This suggests a notable decrease in performance. Similarly, the Squats showed a t-statistic of 7.666 and a p-value of 3.555 × 10−6 , with a mean difference of 3.509286, indicating a decline. The Rows exercise had a t-statistic of 5.9217, a p-value of 5.053 × 10−5, and a mean difference of 2.620714, pointing to a reduced performance. Biceps Curls presented a t-statistic of 6.2337, a p-value of 3.05 × 10−5 , and a mean difference of 1.275, suggesting a decrease. The Triceps Extensions had a tstatistic of 6.2711, a p-value of 2.874 × 10−5, and a mean difference of 1.044286, indicating a decline. Lastly, the Lat Pulldown exercise showed a t-statistic of 4.5751, a p-value of 0.0005208, and a mean difference of 1.512857, pointing to a reduced performance. The t-statistic measures the size of the difference relative to the variation in the data. The extremely small p-values in all cases indicate strong evidence against the null hypothesis, suggesting that the observed declines were statistically significant. The mean difference provides the average change in performance, with positive values in this context indicating reductions on the weight lifted after the experiment by females. According to the studies, healthy individuals are susceptible to experiencing a decline in muscle strength, cardiorespiratory fitness, and motor performance when they cease regular physical activity. The consistent pattern of strength decrease across different exercises and genders highlights the universal importance of maintaining regular physical activity. For healthy individuals, consistent exercise is crucial not only for muscle strength but also for overall well-being, cardiovascular health, and metabolic function. The observed decline in strength after a period of inactivity serves as a reminder of the body's adaptive response to its environment and the need for consistent stimuli, like exercise, to maintain optimal physical health. 9.2. Analysis of the effects of the experiment on changes in body composition Just as strength tests, people also did body composition tests. Using an InBody machine people were able to get to know their body composition stats. They did 2 tests, one before and one after the experiment, and then both results were compared, aiming to state whether the experiment had a significant impact on body composition or not. To compare the results, a regression analysis was performed. The null hypothesis for the regression analyzes is that the experiment does not have any impact, while the alternative hypothesis is that it has. 2 scatter plots were plotted willing to have a better understanding of the data by visualizing it. Graph 2: Body Composition Metrics Comparison for Males Graph 3: Body Composition Metrics Comparison for Females As the aim of this section is to state if the immediate cessation of physical activity has an effect on the body composition metrics, the only factor important is the distance between both lines. Thus, for males' weight, muscle mass, and intracellular water it is possible to see that the lines of best fit have a significant distance between each other. This distance indicates that the data has changed since the beginning of the experiment. However, for the body fat percentual and fat mass, the lines of best fit are distant from each other on a certain point, but they get closer, this suggests that the cessation of physical activity had a varied impact on these metrics among males. Some participants might have experienced a significant change in body fat percentage and fat mass, while others might not have seen much difference. This variability could be due to individual differences in genetics, or other factors not related to physical activity. Additionally, for the female portion, it is possible to note a great distance and symmetry between both lines for all body composition metrics. This suggests that the cessation of physical activity had a consistent and pronounced effect on all body composition metrics for females. The symmetry between the lines indicates that the changes in body composition metrics were uniform across the female participants. However, despite a graph being a great way to understand data, it is still needed to perform calculations to have a more reliable outcome. Thus, regression analyses were performed and the p-value and the r-squared were calculated. After performing the regression analyses for each variable among male subjects, it was found that the p-values for all five models were remarkably low. For the "Weight" model, the p-value was < 2.2e-16, indicating an extremely significant relationship between initial weight and subsequent weight changes. Similarly, the "Body Fat Percentual" model had a p-value of 1.01e-12, demonstrating strong statistical significance in the connection between initial body fat percentage and changes in body fat percentage. The "Skeletal Muscle Mass" model had a p-value of < 2.2e-16, underscoring the highly significant relationship between initial muscle mass and changes in muscle mass. In the case of the "Fat Mass" model, the p-value was 3.6e-14, confirming the strong statistical significance of the relationship between initial fat mass and changes in fat mass. Lastly, the "Intracellular Water Percentual" model had a p-value of 9.78e-16, signifying a robust statistical significance in the association between initial water content and subsequent changes in water content among male participants. Furthermore, the R-squared values for these models were notably high, ranging from 0.9872 to 0.9997, indicating that the linear regression models provide a robust and precise fit to the data, explaining a substantial proportion (ranging from 98.72% to 99.97%) of the variability in the dependent variables for male subjects. In essence, these findings underscore the strong and statistically significant connections between the initial measurements and the subsequent changes in these variables specifically among male participants. Therefore, the null hypothesis is rejected, and we state that the experiment implicated a significant effect on the body composition metrics, as the graphs 2 and 3 illustrates. 10. Conclusion Aim: The primary objective of this investigation was to determine, "How does the immediate cessation of physical activity affect the strength and body composition of physically active humans in a time window of 20 days?" Main Findings: The data collected and analyzed from the study revealed distinct patterns in the effects of halting physical activity on both strength and body composition. Specifically, there was a noticeable decrease in muscle strength for both males and females. Additionally, body composition metrics, such as weight, muscle mass, and intracellular water, showed significant changes, especially among male participants. Relating to Hypothesis: The results obtained support the initial hypothesis that the cessation of physical activity would lead to a decline in muscle strength due to the muscles not being actively engaged. Furthermore, the changes in body composition metrics among males were consistent with the hypothesis, indicating a significant effect on these metrics due to the cessation of physical activity. Significance and Implications: The findings of this study underscore the importance of regular physical activity in maintaining muscle strength and optimal body composition. The observed decline in strength and changes in body composition highlight the body's adaptive response to its environment. These results contribute to the broader understanding of the physiological effects of inactivity and emphasize the need for consistent physical stimuli to maintain health and fitness. Limitations: While the study provided valuable insights, there were potential limitations. Individual differences in genetics or other external factors not related to physical activity might have influenced the results. Additionally, the study's duration and the sample size could be factors affecting the comprehensiveness of the findings. Extensions: Future studies could delve deeper into the long-term effects of physical inactivity on various body metrics. It would also be beneficial to investigate the potential recovery rate once physical activity is resumed. Exploring the effects of inactivity on different age groups or individuals with varying fitness levels could provide a more comprehensive understanding. Reflect on the Investigation: Throughout this investigation, the importance of consistent physical activity in maintaining health and well-being was evident. The study served as a reminder of the body's adaptability and the consequences of prolonged inactivity. If given another opportunity, incorporating a more diverse participant pool and extending the study's duration might yield even more comprehensive results.