The Cell Cellulae (Small room) The Organism’s Basic Unit of Structure and Function Types of cells Prokaryotic Micro-organisms Eukaryotic All other forms of life Cell Theory 1- All organisms are composed of one or more of cells. 2- Cell is the basic unit of life. 3- The new cell arises only from preexisting cell. 2 1). Prokaryotic and eukaryotic cells differ in size and complexity Similarities • All cells are surrounded by a plasma membrane. • The semi-fluid substance within the cell is called “cytosol”, containing the cell organelles. • All cells contain chromosomes which have genes in the form of DNA. • All cells have tiny organelles called “Ribosomes” that make proteins. 1). Prokaryotic and eukaryotic cells differ in size and complexity Differences • A major difference between prokaryotic and eukaryotic cells is the location of chromosomes. – In an eukaryotic cell, chromosomes are contained in a true nucleus ). – In a prokaryotic cell, the DNA is concentrated in the nucleoid) without a membrane separating it from the rest of the cell. – In prokaryotic cell, DNA is a single strand or double strand DNA. But in eukaryotic cell, DNA is double strand. Prokaryotic and eukaryotic cells differ in size and complexity • All cells are surrounded by a plasma membrane. • The semifluid substance within the membrane is the cytosol, containing the organelles. • All cells contain chromosomes which have genes in the form of DNA. • All cells also have ribosomes, tiny organelles that make proteins using the instructions contained in genes. • A major difference between prokaryotic and eukaryotic cells is the location of chromosomes. • In an eukaryotic cell, chromosomes are contained in a membrane-enclosed organelle, the nucleus. • In a prokaryotic cell, the DNA is concentrated in the nucleoid without a membrane separating it from the rest of the cell. (A)- Prokaryotes Micro-organisms Types of Prokaryotes Prokaryotes Bacteria - Exist in most environments Archaea - Exist in extreme environments (hot and salty) They are differing in some other structural, biochemical and physiological characteristics Fig. 7.4 The prokaryotic cell is much simpler in structure, lacking a nucleus and the other membrane-enclosed organelles of the eukaryotic cell. • In eukaryote cells, the chromosomes are contained within a membranous nuclear envelope. • The region between the nucleus and the plasma membrane is the cytoplasm. – All the material within the plasma membrane of a prokaryotic cell is cytoplasm. • Within the cytoplasm of a eukaryotic cell is a variety of membrane-bounded organelles of specialized form and function. – These membrane-bounded organelles are absent in prokaryotes. • Eukaryotic cells are generally much bigger than prokaryotic cells. • The logistics of carrying out metabolism set limits on cell size. – At the lower limit, the smallest bacteria, mycoplasmas, are between 0.1 to 1.0 micron. – Most bacteria are 1-10 microns in diameter. – Eukaryotic cells are typically 10-100 microns in diameter. Cytokinesis in animal cell: • Cytokinesis, division of the cytoplasm, typically follows mitosis. • In animals, the first sign of cytokinesis (cleavage) is the appearance of a cleavage furrow in the cell surface near the old metaphase plate. • Cytokinesis in plants, which have cell walls, involves a completely different mechanism. • During telophase, vesicles from the Golgi coalesce at the metaphase plate, forming a cell plate. – The plate enlarges until its membranes fuse with the plasma membrane at the perimeter, with the contents of the vesicles forming new wall material in between. binary fission in bacteria • Cell division involves inward growth of the plasma membrane, dividing the parent cell into two daughter cells, each with a complete genome. B- Eukaryotic Cell 16 Internal membranes compartmentalize the functions of a eukaryotic cell • An eukaryotic cell has internal membranes, which partition the cell into compartments . • These membranes also participate in metabolism as many enzymes are built into membranes. • The general structure of a biological membrane is a double layer of phospholipids and diverse proteins. • Each type of membrane has a unique combination of lipids and proteins for its specific functions. – For example, those in the membranes of mitochondria function in cellular respiration. B- Eukaryotic Cell Eu: True Karyon: Nucleus Animal Cell Plant Cell What are the functions of cell organelles ? Compare between Animal and Plant cell? Euokaryotes: Euo = true karyot = • nucleus . Plant and animals have real nucleus, surrounded with nuclear membrane. -The Bacteria and the virus’s have • no real nucleus they contain nucleiod region (no nuclear membrane) were the very simple genetic material (DNA or chromosome) -The prokaryotic cells (bacteria and • viruses) also have a very simples cell structure cell wall, cell membrane, cytoplasm, ribosome’s and nucleiod area for a very simple genetic material (DNA or RNA) and cilia or flagella. The euokayotic cells have a very • complex structure and many cell organelles (Look at the book page 112. 6th ed.) Fig. 7.8 Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings المادة الوراثية النواة الشبكة اإلندوبالزمية نوية الجدار النووى سوط حركى جسم مركزى ريبوسوم حهاز جولـﭽـى غشاء بالزمى حلمات دقيقة ميتوكوندريا جسم ُمحلل 20 الهيكل الخلوى فجوة مركزية بالستيدة خضراء الجدار الخلوى ثقوب بينية 21 Prokaryotic vs. eukaryotic gene structure prokaryotes: polycistronic transcripts eukaryotes: monocistronic transcripts 22 Prokaryotes – “Prokaryote” means “before a nucleus” – No internal membrane-bound organelles – just one little bag of cytoplasm – No nucleus – Usually single-celled (may form simple colonies) – May or may not require oxygen for survival. – Earliest types of cells on Earth – Cell type of all bacteria and Archaea Much tougher than eukaryotes Can survive almost anywhere – and do! Have much greater genetic diversity than eukaryotes Have a cell wall surrounding the cell membrane (different chemistry from plant cell wall)