Isolation and Extraction of catalase from Animal Source
Catalase is an enzyme in the liver that breaks down harmful hydrogen peroxide into oxygen and
water.
Catalase is an enzyme found in nearly all aerobic cells (animals, plants and microbes). The
function of the enzyme catalase is to break down the chemical hydrogen peroxide inside living
cells. The function of catalase is to protect the cell from the harmful effects of hydrogen peroxide
generated as a by-product of cell metabolism.
Material Required:
Beef liver, blue capped tubes, 5 ml of hydrogen peroxide, centrifuge machine, test tubes, water
bath, filter paper.
Procedure:
1. We added 5 ml of hydrogen peroxide solution to 500 mg of the liver tissue and placed the test
tubes with samples in boiling water bath for 20 min and liver is dissolved.
2. The catalase in the liver decomposes the hydrogen peroxides to water and oxygen.
3. We gently transfered the dissolved liver into a blue cap tube.
4. Then we filtered the extract using whattsmann filter paper and the filtered is collected in
a new blue caped tubes.
5. We centrifuged the filtered at 12000rpm for 20 min at 40 C.
6. After the centrifugation, we took out the blue caped tubes.
7. Carefully transfer the supernatant into a new blue caped tubes ,the pellet is discarded and
store it at 40 C in the refrigerator until it further used .