Test for glucose (a reducing sugar) 1. Add Benedict's solution into sample solution in a test tube 2. Heat at 60 - 70 °c in water bath for 5 minutes 3. Take a test tube out of a water bath and observe the color A positive test will show a color change from blue to orange or brick red Test for starch using iodine 1. We can use iodine to test for the presence or absence of starch in a food sample 2. Add drops of iodine solution to the food sample A positive test will show a color change from orange-brown to blue-black Test for protein 1. Add drops of Biuret solution to the food sample A positive test will show a color change from blue to violet / purple Test for lipids 1. Food sample is mixed with 2cm3 of ethanol and shaken 2. The ethanol is added to an equal volume of cold water A positive test will show a cloudy emulsion forming