'lpE,-,o' : REXRESEARCH P O B O X1 9 2 5 0 JEAN NV 89019 USA irR, e8,ec7 I'tLE: €c ecrrt.o.Cut-lu,(€ +l 7o ^, tr $ r{ 2y S c t .t/€I,J " \ r y J t i,. r r /. o )tl . pDo Making rain while the sunshines 'Flrr.Efense 'S soh a letl b"tq'iorllouaoi d.! ald ard ^ntAar Dan .g!!ryth ,I lDe 3ld gr€elhqrs€ svathor loot,Wry co.. Beoedld!!n, -Ite ha} gudan 8und8td" gtme h co6d ad h Feun|lary dr.ldt it bofr Ub t lsr slinold€d grldf h,roybern l aaorteri 'Tbh bio.rgulatd Eutreeearctec-sy apt€lls ta havt sldo b Ecdodb cofrD th poteotirl the e Fteo$al lc blochnied blochenlcd maaipnaDiDo- '.,.Se $aylte |€artbiriliddteI l8d6 of hd6 o{ plaotgrmil rld bsvet profaud ofouadlmorc't lnprc't or lacre or.ln ol0ty rld tld g![ty of crld $ar6ty ge roalot[ef rcae ttcrt Lrv! iot|ld thet DCPTA BloregulatoF give u tle pokothl E€m lcdvity i! the phot, speediDt!p tb€ productiGol €dym€stbat lD br! tor madpulatrng for maniDulatinrtle tI€ ge c@ re$ilatiod reflilationo{ planl B€ocdictsara spe€ddp tie plaltl n€tabolbm. e r DIaDL' sa!r€."ald co| d "and conld Tbey first dir(lY€'ed lhrt DC?TA havea prolourdimpactooagricultural darbledthe rutber productiood gua. cropproducuvityald gualiry." EVERYDAY Some schntists have maintainedthat cloud seedinc_ the practiceol disp€rsingchemicalsinto cumuluccloudsin 6rder to ma*e rain-is about a! effectivea.spalng someoneto do a rain dance.ibr midwestemlarmersand water manAgers,however, cloud seeding ha.soften seemed to olfer hope in times ol drought. North Dalota larmer!. lor instance.hive had enouch confidencein the procedureto target 6.6 mi ion acres lor the "store-bought" rain at the cost ol 5c an acre, a state meteo.ologist estimates. Skepticshave neverdoubtedthat whensilver iodide seedinc agentscome into contactwitha cloud'sverycold moisturedrogi Iets,ice crystals-$fhichbecomeraindropsin warmweather_ are lormed;in laboratorycloud chambersthis is known to happen. But could the processwo.k in the field_or, perhapsmoie aptly in this case,in the sky-where the real cloudsroam?The skepticsdoubted that seedingagentsdisp€rsedat the baseol a tall cumulus cloud could wend their way5,000to 12,000leet up, to the part of the cloud that containsmoisturedroDlets. Last month. in a collaborativeeftort.scientistsfrom the North Dakota WeatherModilication Eoard in Bismarckand the National Oceanic and Atmospheric Administration (NOM) in Boulder,Colo..took to the cloudsin orderto settlethinqsonce and lor all.The researchers released a tracergas-sullu"rhexafluoride - simultaneouslywith the silver iodide,and then followed in a secondplaneequippedwith detectionequipmentto monitor the tracer'sdispersal. 'The stuff had a bit of a climb to make,"saysJohn Flueck,a NOM researchscientist,"but our preliminaryguessesare that in at least one instance the seedingagent was successtulin reachingthe levelwherethe cold wateris."Theiesearchersalso testedsilver iodide with sodiumchloride.This combination. sa)6 Flueck,"works much more quickly-because the water in the cloud doesn'thaveto be as cold for it to work.. 8,{5 S C I E N C E A N D M E C E A N I C S f o r O C T O B E R '1 9 3 5 Sky-Seraping a RAIN is caused, as everYone knows' by the upper eir's becoming too cold to hold any longer the water it contarns. the idea that rain can be Consequently, made by firing bombs into the skl" to shak€loosethe water, is impractical. A French meteorologisl Bernard Dubos, proposes to produce lain bY l€nding Nature a hand; in othe! words' helping her to carry out her ro!trne processof drawing h€eted,v/ater'laden ;ir from the surface of the earth, and cooling it (by expansion into thinn€! atmosphere) until the rtatel codes donrr again. His project, illustlated lere, i6 to Dut uD stupendoustowers of concret€, i o l t o w w i t h r n ,q h i c h q i l l c r e a t ed r a f t s in the same manner as a factory chimney. The ascending air column will cairy sater uP \*ith it, as vapor; and' *.hiilins vanes s'ill distribute it in all directio;s. By this means, M. Dubos believes, the natulal moisture ot the ai! can be readily increased. Such a tower, steel reenforced, and two_thirds hlshe! than the Empire State Building' would cost about $10,000,000,it is estimated, and be of gr€at scientioc as weII as climstic value. Torvers to f,Iake ltain An .nsin..tr pl.r t Ettli:. *intin. prin.iDl'., lor i'rrltti'r clii't'' iir;i=ii;ef s'rrs liilHil;ii:Iii::igiii?i:: ; : i; iF ;' l :t6+ ? : :9i - : ? "->. ' i ; c ) I1 !,tt+1::; il,ii::iIi": :;:irs;i;iiliiiif Fii 1iltIiiiIrg :11=::i: i3ii?ii''u' li;lutili;iitil i; a'iii;ei; €'3 F{? ;r; ie igli i:1i i 9, r: 8s 7 *E; E p9 ^:. o. 5;i = c: -7 < G ' = g - -a' -l F E'. t€6 A :.- ;' 1g 5 :.* & J ='a i q 5 ' r . t-- -ct r The Electrificationof Seeds?;_1i:=,:iz=+ii . A Revolurion iu Ag.iculture l$,.By Cl-rarles A. Mrrcicr,N.D., F.R.C.P., F.R.C.S. C:i;: ol:i;! ;;r; j ii: tiE fi;i F 6 C N c- 1Z < - : < : :I - ;;\\ t,o . 9 1 e q i s:. .' 7 ) 3 : ' E a iiiii!i I ;ifFe 9 > i:' s 1 ::t: gi >: 46 -- ; =J J) ; iiirEi i.ii;ici 1:iiii€ "jlu, i i iiiiii::i ltr| zi1:,2= i? i;!1+?ttt;,z = . =:1! t i ii1i ii;;;t: ii:t'1: i i,;riiiiii' i;il:;iiii; Iliiitii:?i ;;?:1:i?iiit1 i 3Ii ;g:; Iaiiti =ii;zi??i3 ii1Tii{1ti1 #i#iiiiiiiiili i s: : ii u i iiIii7;!11i1:i iii*r;iiii rr;c,isiiii+*r;iariiiiEg: t- i;-rli'i:li r ==r EI;s-= ;;lili;;::;:l :::; :1, ;;;iiIi;; :::l1;; iii*d=i iii:;;f:fii{i ;iri:liii |0 E?17 ugiliiiiii i +'7s i* i#i ;iiii i I i iiiti ia ;zt++ztr;iiiif iiiiiii#ii**i*i# ",:it:lii :!i::::' i:iiiiiii ;;i+iifli;;ii l;i;li€ ii;iittlili:E;Ei u " u= i i ;iii::ir;i iiiii -;#iit{*iiii :it z?1!?i t ??i;i i72zi?7zi! :l:;:;::;!{l ;ziz;ti:ii* ifii*IIi:E 7!1t1;i =iiII !11112112!t+1 IiliIiIi liiiiiiiiiiiii ttitT::ttl Ili=iIiiii iii*ii:ii iii 2 ! * i r ^ ! i" it q3 !e! 8 , i r s = : ; * 6 2 - - c - =? -g= , i - l i;;::: i-:Eii:1g'?,; i; = + 8 6 * v L a A : ' - T ' 3- = 2 1 : . -E E i ' 3 : . i =s € ^ a : ; 3 e 3 ; ' . 3 ' Ib g' 5 E - " ', , i i r6;2- ii ; :a1 6e 1 :z i :: =3 4 i 3 It $ill talc mrrry ).crrs heforc all thc behc6t that cnD hc obtoiled frunr thc process is finall-v :rrrivcd :..1. At present;it h; bcen vorked out onlj,for.rvhc:rt, oits. aDd l).rlc1; Lut cxperirDcntshave shorvn lhat it.s $dv.ntagcs extcnd to maDl, othcr kiDds of sccds. Trrrnips and olher rooLs, rnrizc, ricc. cabbhgc, aod ntany otlrci Irl1rr,fs gro,yn from sccd arc kno*,n to lrrullit; irut for thc;c rc prorcs: is loi lct rccorn,ucrrdtd,lrccrrrsetbe .r_.:., cl cc,rr{iiiio,rl },lrr(, nc,l. l_,crDrLoroir,:llr r::L.r.rl i . r r r < , 1 t L r . r r , r , . Lr c r i . : r 1 1 : r l , l e lrjn.il r r , i u l l s h x . , c b c c n ( i ) i 1 1 i n( l f r o r , ( i l ! ( . t r i f r inA scc(l nctrloer; brrt ljrc resrilt.sr,rr:rr,,t ].et sum.icntl) unilorr,r to jujtjfl its apl)!icalion on:r lergc sr...let!r I,otetocs. I _ L , r r i f i c r t i o n o f s n , . , l sr r i l t b c : , , 1 u r . , ^ J on 11\'erli largc scalc in Ilrrgland for Dext year's Larvcst. Dvcry larrno nho leg ^ ^ -,. ^-:-i -. tried it oncc upon o srnlll acreagcNill usc 7 - ? . * - - < it frecly; and though it has scaicclr.b,.,cn : ! ] z l i l a<lrnrlised at all, fnrmcrs talk to one another, tl,cv sr,. the articlcs \r ril,ten by orpcrts \rlo hsvc inslrcctci 6 : = a = = a ! ; + - > r t : : . + . 7 thc crops; &Dd the ,lmoudt of secd that will be 7 " . * 4 " " A 1 a . . 2 4 - ; : *1? Y": -l - - :.rj :5 elcctrificd for next yeer's hqrvest $ill bc oracticc r : r: i l' : l : 5 ; i - "! ; i ; ally Iimitcd only l-ry the pacity of tbc l,lr;t ltrid t' 1 a r a = a ^ 3 i : - : don-n- for the purposc, In Great Britsin the proccss is = -+o 3 i:\ g ^ - _ eslablisbcrl. i '_ So cmphnlically j ; 9 l ; ! E truc is this tLrt Dr. \\'ra1,,the I'nited Stet.,s- Insyrcctor-in Chrrgc in Crcat Britain, aftcr an extendcd tour of inspcctior, of farrnq he5 adviscd his Gorerusrent to havc imrncdirrte trills uadc:'n everv 3 ': : : :-3 0 c - 6 a - ; - i - ; ? ' * : q ; ; " , Statq of the UDio,i. 1o my mind, the tlne for i,rialsis I : 9 : past. l'urther trials are but s'astc of vlluaLle tirnc. j ' ( : . ; Z1\'e must flrce thc fact thr.t thcrc is a ehortoge of food 1 = = 5 - Y = ell over tl:,' rsorld, aud that this shortege uil-i bc nrost - 4 Y 6 j : r r t : - i - j t : + ! c i: -3a j=< ..3, s cj "g A " ; = a " - ? 2 {rcrtely felt ncrt Jr3r. It is ncxt year th.rt th(. jncreascd ; ' l a 2 - : " a 7 =.= = l, -'? ; : 2 r a : F 1 ' ; i ' : lrro.lu.tio[ will Le rrr;uired; and furtLer trials. rhicb :*oi:1:6. could tcll rrgDom,rrc lhrrr rvckno$ alreadt. r"ouid onlv I Y i : - ! - : j ' _ : " _1 ; l : i : a : 1 * - - ? = - - . , i = ' t JrcslDor: lhe edop{i"n of lh- tro"oss for-another tca:. " ^ It is lfaDl.cd !oN, and badly \!anted. i t.iti.= -. !')"' t j.; l -;i:7?1?i2= Er=i-€ii; Aiii="'=ZF: i 1i;:Tg: d:;g,: r i l ' ; ' : ;;li:iaills -'..; = =1iti;: ;; i1; ils; ::x;iE: 3:':i-.i !+eEE i+l;:l:i; i+i*;liliir;ii:i :i Zi . i =-:i; l'r-:==izii:' i fI lli!3:-S:= A= ;-i;= ziiti?;gir! ii tt:zi;s:: i :,i,: = i i i':;.' F ---"4 iriiii3rs riii =13-?: :: 9 9 ";i : i - ; ;i , i i ; : 3 i r l ; ?z 'J?it:ii-,izl #: z' 7 z+ :.r -n:: .:A-na-,') a ' J . - =1 ; ' se a2cA- >a )q E ?d = *ii ? d ; ; i J - c i A . F r r s i i:5T il Ei ureS 6 :o.e2z1c 2 iii,i,::!: Ei:;:l i:s?? ;:; o gF : 1 5 !H : 7?Pe.e .-a=v4 ,ruffruffi E; E;€; ; : : * : c r = : !g ! e';:E? -"rgH;E 4 = ; : f c "tG'Etn !5:3.:i E7Ut2i 1E:3:? ?-;.i.3iz )22r8 v.e !ri 9 ; .i 422 a a |: l:Ei!:: t 4 d = 2 - a i.!;:i:i: !t: egE-e,3 X! ; a; 5 ' a = 9 a a .^=) it !iE!!E; ir 8-io 5 E"-cs sE :6!: 6: zi s€ii ri;, za) a€- xz isEgEi tE; uE l! 9 !6 t Fo : ; 3 8E 5 E i v"i q! ,.c; =9 : z i< ! 1'l |5i.2- "_ I']i > -;7o . o.i 9Z Pt a4i - a z. ? x Q< rP-^A:! :5. "pi E sE:Si"i :t7=iZ J. Csrmen Garcia holds sn ov€rsizod burrch of co{arct gr€€ns in E t 97g Dhoro, vfl 9 ri i ' z ; E.l a :zz u Z V i = 6 - Gionrvegerqbfeffi 3:!E: F; ::.'E;; E o: o I o: ::EiffE C *"lt'"q.'*'l#ry:*U"',g:1il1.*:: fr:il,,T"iiiiBfll;,r",;X:ltbg,U;., ) ffiffiffiffiu*ffimrum ;#trTi$.1;fn $ir-r:*xt-ls:rfitrrld;+ po.und crilc|;i;; "'t''t'' re€prI Irts sl|tet.'' c8r i;;l;'rt;' roFebruary, u,iii;f;;,Jiiiil#t,i";1,?lifl;,u ST,*r:il,l paDerr $ere Elcctrlclty a! a Tre€ Pelt Cur..-ltteDt recently grantcd to Isodore l(ltsee, tr Pbllodelphlo hreotor, caverlng rt proc$s for the dertructlon of lnEect ond germ llte bnrmful to pl:rnts oDd tre€s, tbe €lectrlcol nrethod tnklng tle plocc, to n greot extent, o! tlre usull lprnJ'a |rud otller.nnpllcotloDs, Thc procers c$uslsts ol Drnklng {ln nDpllmtloD ol o loltrtlon such trr stlllDe r$ter \rilrcre the grouhd ls to be trefltcd, lrud tbeo cltusIng d,current ol electrlcltl to be D{ssed throuSh tbe soll, n'hereupon the gns generatcd $ lll rld the soll of gerrhs, hrrne oDrl lnsects wltlroot the l€lrst lDjnrf'to the rege, lvherc n Inrgcr nrcn ls to bc trc^ted, lt ho! tltlon. lFen found desllnble to dlg 8hollow trelcLes nt opt]oslte aldes of the flrclr to l)e treflted, Itod tie electalc ternrlnnla nre nlnced ln these. An oJ|lllclrtloo of e 8otDe. $.hnt rnore porverlol culretrt rvlll rtd the eDtlre areo ol lesta. Wlren the l)l{nt ltselt lB to be trqrted, tbe aol[tlon cnrrJlng ihe elenrent ls lldde the electrollte ln on rppnrntus, nrd the Jrlflnt sprirled vlth n solll oD lftcr deconlposl orr has tokcn place througb the dcflon ol tlle electrlc cuneDL IL\lllll:l-it, 1o I)t.ts1-n()\'lrr; t\sl:r"ls l,ti,j,3rii I , 1 , , \ \ 1 . t , , t . : . I s r j r ) nI i r r s . r : . I r , i l r , l . l t h i r .I ' r I l l . d 'loj-r ) lr'.! r:. 11)l.l Srr,rl Io. siri,Srs. 1Cl. f<6 ze \1tc ',f rlr. Jnil lr,'n incr.i( nrrnrrrrl to I In tl),,tfrilrins rl)r\i'l\ in Irotl,llng lin.. ll'. hrrll.,l \|li'h \'rflxln. rl|. soil s ill\ r.,,rrrp.rit'l .irr\inA in (nrirl'1nrlion. !.rrnl ( i , l . r . l . n r " n l . ' l r r r r o l r / i n . l s r i , l 1 1 , n r p . r r r , l$ h i l . i r s a l ( j .0i1. th.rfl'r fr.' lnA sil{1 .l, rr, nt nrrrl rnrr\lrr: tnrouCl' :Ni,, .lrnr' nt tb. rl'str[, ii.h rl olirro\l'u\ jri!'\ ls. 2. Th. rri thorl ,if ,1.\tr.r'iri;t .l no\ious ln'erts lhclf \ | l i (l ' ' n D ( i 3 \ ' n I n i or .g:r Inf..liria ln, t.il lirrif in Ins rtl rlir (,iil wllh i !,,lrrri.n orfrii: tr.:niling , i , 1 . i n ^ , l ' , r' , . r r t . l i r . . . 1 . , r , l \ r i : l l r ' ( ' l n l l o n ' l t r r i n F jrr cr,,l s.il llrr'rril lr..in{ th. tlnrc lliil ihf \.trr. ll t n , j 1 n . , . (l l , i , l r f n , l n i . l r l r ' g l l r . ' r r r r f . r r r l r l ' l c f o r l h r ,1.\t.u{ li,it .i srl,l in.c(ls : 1 . l r r l l 1 , 1 , \ t r r r ( 1 i . r. rf i n ! . . ( \ o ' n n \ i o { ( t d p l i n t l i l r ' t L r , l r r t ) f o r , r ! r i r N l L i !l . ' , r r < i ' r \ 1 r . l ' ( i r o l l z i n j . r ' r q r i r c r l lhr flints rrtr (4tr:rng ..rnDor,ri,l. ir lir \dll fifftrn! lftlorr. tlr.ou:.:htlrf (r\.s frff,l 'l r, ',: rl,r .l'!'lr.lrrl. l h , ( l . s t f , r . t l o n o l l l i . t n \ ! , t . l r r l f . l l ' r s s 1 1 ! ls o i l tr 'i:Fs oI nrrrho,l ol rl,\tr.rirr.l tho hr\D 4 lhf thr n"r !n lrorini': ohhoxl,,rr\ irrsr(t! \'hl.lr r"n'i'l{ nr .,r:i Nlrh r ronr!,und crrrv lnlrr,il.,l t)! sNll lif!. in:: in , h"'rrl,rl ..f,l,inrri.n rr Ins'.tir t'lr cl'nrcnt lrnd lr'!i1.c s^l{l ln ! l..trnl!rin: sii,l ..rnni,:tri,l. lli.r!ir \".ri,1,1. "r.'n. nt. 5." ftr-"r. f\"ADj nrc r s't1 :Pr iip l-r +Y I Pt 3 o : r!, o e.9 aYl lrl E3 : : ! i + E :E=? i 5i r gE s€€ !:j r:E=i:I cr;! 5,' ; P . - : o l E - . 1 : s : F ^ - ,e F ".8 !:i+i1'E€;;E;:;gE (r) r-1 € q i : Eg E ; :; ii }€; E ; EE? ! E E * e l s o F z :T;i; :'c E Z '* .eb O F Q trl Jr r l 3 a ; : i e;ib:;:, = t i : : ; e i ' E . E ; ia. tr t ,:*"i-;i [:;!E€H;i#E:F=5*E€ U F LII'EEE y€*eE il'A3:€!E; e+{€i l.e5 .s5 !.! .aa .:i i"B * ! T!: vz .@ tFEEi$iffi $$giFiliiSft lr?FgFlt +^Pl:giE-si liEF,i+;iili - iliiiili i,!E*egi; FiEFr.q;r -- o *g 6 h6 E € t i+E ; r E : t llir Fltieil: 6= * x I *;9 { 6t ? o a ! r ; i i i o x : x i ! ) iil*iilli ;iiiiii€ii 1;iii:ltii i3i.i9i?;g Fenee Shoeks Flsh s* iiri;iidEi:r *} ilffilEl;Eli - g l r p s a i i ; 5gi iilt*fii :, Otrr in Califomia, foolish 6sh who b-.vto s\\'im into irigation ditches are going to cet the shocli of their lires. A marine verlion of the electric cattle fence keeps ffsh out-$ithout hurting them-u hile iassing Nilter or debris-Shoqn at right. it consists of a free-srvinging rou of electrodes.connected to a Senerrtor. The electrodesslightl r < l r a r g ct h c r r . r t e ra r o u n dt b e n r . lnrented br licnrr T. Burler. of Hcrllr'smd. C.dif.. tl,' fisli screen u\es a qenerirlor he derel,,ped iointlv sith \\-estinghouse. \ettings in Califomia hle shosn th.rt nt times as mrn| as 5.(D0 sLLlmon have been krst in a single irrigation ditch in a 2,1-hourperiod. Hvdrclectric plants and industrialpumps are other hazands. 9{ rorur^rsc'tnct r \ , r Ut1 '{7 I t' +w oVERrlnEs ENERCY cttLoRoPHYL Dr' T' G' lltcronvtua About 1930, I dcctdcd to trt rn GrP'rlo€nt of I hed bcan conducttnt Chlorophyl Dnct3yovcr vlrcl. conduetlnS EloPttc lnct35r ovcr lont dlrt.nc' vl' vlra. A vood Plrtfon u.. tnrt;llcd on thr touth tld€ of th. houac .bout alr frct rbovc Sround. L'tcr that th. ?htforr lutt bc rt .rp.rlr.nt. ft$lcetc 6' rbovc around tn otdcr to let tha declrcd lcirt potcntl.l of rncr3y ntlch tncrorrca Ylth dlatrnc' abov. lround. I cut nivtni rooc modcn ctjrr boxc. .v'll'bler th't boxrl .ttht boxar eprri and cut glcccr lnd rdc rork' rlll borcr .lt. rcr. 2"i2'.r4" rl thou;h rny Alualnr.! foll vrr plrccd on th. bottoo of lcvcn to b. tn cont.ct rtth thc 'o11' boxcr lnrldc ro.. o f f o ll rcrc alrccd on thc und'r 'ld" Slltlrr Pl"cca of thc lid of crch box. Ilrca vrr. conncctcd to crch plcce of foll, thr ylt.a ftoE thc llda rcra cxtandcd to thc run plrtca, thc rlrcr fron thc botton fotlr vcrc connectcd to thc ert.r plpc rnd thur groundcd. Scc flgur: "2" lor dctrllr ol the box c o n at r u c t l o n . lGf"r to flgurc rrll ytlch ahou! e rrrldc" vlcv o f thG lnrtrllrtlon. lltur. "3'r rhovr thc ryltco o f c o n n e c tl o n r . rcrc plrccd on th€ pl.tfortD ro rE Seven pl!tc! p l c k to up energy froo thr lun rnd a gtrc r.!! connected to each plste rnd extend€d dovn tnto the brret|lent, cach box, hlvlnt the top foll plltc connectcd vlr slr€ to ! plrtr out on th€ plltfori! ln th€ aun ItBht. Ttc cl3hth box hed rp connrctlon to th. out!ide, lt bclnt thc "controlrr. Th. plrtcr on the platforE ecrc rll dlfflrcnt r l z e . The rrnellert urr 2"r4", thG n.rt 4"16"' tn thr' lrrgcrt vo! .bou! E"r[0", rnd ona glrta val coPper acrecn ulr". S o t t t rd l r t e t a r c r ? . n c d r n d | r ' o f d l r t p l r c c d ln cach box. Ort lardt Yala ralactad, rll of unlfon rlrc rnd plantcd ln tuo roea of 5 acada rprccd ln cech roe, thrn l" of dfrt uar pleccd on toP ln th. box, The rlhe rnount of l,!t?r uer addld to cach box al needed froo day to daY. All of tbe t€cdB sproutcd rbout the srrtle tk c. u Then r notlc?d thet thrte uet no chlorophyl ln the l0 plantr ln the control box, All of the boxes connected to outrlde Pl!t€r hrd Pllnta rrlth nuch chloroghyl. lh J3r? qutt. Irrgrlrad to rDt. ahat tht plantr l n b o x c r v l t h l o r l c o u t a l d r . p l e t r r , r c c r n e dt o l o o k rt lf th"y b.d btcn aubjcct.d to h.rt. Apgrrcntly th. l.r!. outrld? plrtrr rcrc brtngln3 ln rn .rc.ar of .ntr3y coGprrad rltb th. aft.ct of 3h. .n ll alta outrld. plrt.!. Y.ry aoon, th. Dl.ntr !r.v too t.ll fo" thc ar.ll aDunt of rhetd rooer 1n tha borat to arch bor vrr cqulpped rlth a aprc.t to r.la. th. top of .rch lld up rbout 3/4". llrc borcr urrc pleccd on r rh:lf tn th. a of th. b.t.ant slr.rc th.r. Irt ltttl. ll3ht, rlth |F vlndorr .t th.t .r{. Alro, th. .htlf rrr l.pr d.st bo.rd phcad ts !roa3 ar|d .noth.r on coP to bt. .rclude .ll lltht. lh. phntr r.r. d.rt .ll of !h. tl...rc.pt dt.n th.tf rert trlatrd by flerhlljht. A frt.nd trl..d to dupllcrt. thc .rprrt!.nt bu! dld t$t tolloe all lnrtructlonr. lhclr brr6.nt vrr only rbout I t.Gt froo brecrcnt floor to 3round l"v?l outrldc. lnat.rd of ptrclnt th. out.t.d. pl.t.r rbov. lround 6 loet, thrt leld the r.trl coll.ctor plrt.. on e borrd thrt l.ld dn th. $ound, thu. th.y dld not hrvc thc potrntl.l dlffcrcncc bctuc.n outrld" coll*tor and tnalda borcr rnd thc crp"rlDlnt trt I fellurc. Al!o, th.re 9rr I rlndos nc.r thlt l.t inuch ltght lnto r.filr" tht box.. vcrc Alrccd. Anyon" vio exp?ctr to dupllcetc rn Gxpcrlncnt rhould be rura th.y knor rll the f^ctorr rnd thrt thcy follor. th. lnltructlonr axrctly vlthout rny subrtltutlon or chrnge. And rr to chenges, lf you .re trylnt to R?t r rpcclrl rcrult and are trylng out lcveral ncthodt or ldcet, one of thc cordlnel p o l n t s t o d o l n t B o o dy o r k l s t o r l r k € J u l i i O N Ec h r n g c rt a tth€. Th€n you knor Jurt rArt th! rctultr rr€. lf you nrkc tvo chlntcr and thc rcault lt ldlurc, you do mt knor but thrt on" of thc chrngaa rey hrvc (rcr Dlgurar tlr2ril, bccn rll rljht. n.rt 2 p.!..). ly: r Dl. l. t t GALIN HlElO[IxllS a a C"r?frfc r , v Bortll 7 lr,tetti 7. Uoalsxlr A?prrr ?, tf.',.YtOr+yt fl,m Floe 4 ! :itir.6" l!lr ll na.. fiAd rf 'lls{..&, f ! trtt'rrt |-rterr t'331!rct fua< , Ulll FrfyXg-1r : Il -l . t I I AfrairorrFathn os t/oc,l lcr \ tal\ r{rrr {o .fq Plrt r lic t,/ lilrvr2 CrrrrrG9t's F,.cgil I Alurrlgrr FoiI trisrdc.rlcl bor h I \V,re lr un{u?,7r ' Al,,ledtt' fraa 11\///sr"r nav' s"qda{e lR F:1..tt')' fasvlofc0 U)itr r I I I C',oya)f.b; [ - i;";;1 Kl\xtft F I I €Le C-(<oUc F (R-€ - ANff K \LLER a6Beruon Eledronics\ Route l, Uox 143 . Nlxotr. TX 78140 V S P# + L 6 I + 3 1 INSTRUCTIONS FOR USE For your convenience. the coil wire has already been connected to the negative and positi-ve posts of the transformer At the other end of the coil wire, a by the wing nuts. cut away to i n s u lation has been partially o f smal-I amount C o n t i n u e t o c u t a w a y the insulation i n s i d e . expose the wire w i r e e n o u g h i s e x p o s e d t o reach around u n t i . l in this manner p i p e o r a n o t h e r w i r e f e a s t a t twice. This your post, tree, You may want to use forms a coil- which no ant can cross. some tape to hol-d down the loose end of the coiL wire. For There are many more uses for the control box. Just connect instance, it can be used as an el-ectric fence. one post to a good ground, (either post - it doesn't matter), rnd f ho ^i-hor a fence wire crs cr other ninc LcrLLre n.\cf - f- (^J ty .L^ r! ur ,r from three to five ^.nna.f P!!ru L v. rrn. Lo 'r'h i - ..'i -^ wrrc € . L^ E- ^l i^L E miles n^c I IJVJ f .\ '..i 1l wr._Lr To be used distance. : . ' v. 'u\ u ^.1 u ^1^^!-i t,, E_LELL!rry nr^rrnri 9! :n.l tha to a wire running through a piece of' one-ha.l-f inch PVC n€ rrh: f p\/pr 'lonof h r-onvenienf is r'^,, for Tho o.r't ^F that wire must be exposed and attached at the end of the pipe which wi]l make contact with the ani-mal. This wi,l,1 not harm the This uni t puts out approximatel-y t/100 of an Amp. animal. A I W a y S m aK e s u r hnnk-trnq- or S h o r r ' ld anv aDDlication fh--a f ho ln it ho:nw thereof, ic rrnnl rrnnod rytlpcf i/\nq pfease feel free FOR MORE INFORMATION CONTACT: BENSON ELECTRONICS Route I Box 143 Nixon, Texas 78140 (5t21 424-3373 - --, hafnro .on.trrninr' .m.: .l -z ,i . ,n.n. " -r r. 1 y thic to write. rr-it *:i:i', xit; - ; 'nr . ^'': t i.''.+Y; yaard'vart, which usesan eleclrical cha'Ee to de'lroy znls' lim Eensondemonsl'?tet the Ben",ng.; € ^"f.-e PelS€ C TK ) e,JC,. zto t1V{ Yaard-Varkinvention Benson's ridsfarmersof annoyingants ai leasl b o r d € r i n g r h e G u l f o f M e x ' c o T h e r € s n o t a n , ! _h e r e . and in Central Amenca ano norbing I can'! reach wth mY Mexrco. aU areas \r'Llh a treAbour !q'o J-ears mendous ant -proitems' The Bv Suzanne Htlliburton si.s$ u'riter BRYAN Lm R-n.., Plar_Pd'osl r(r'YUc "^ rn.re no oue:rrontrs i,?!,"ffi;.-a-'-c '. sPl R'beri BPnson - i Fr;iffi:i;'lt-.i'-; b'rn rnundated *"'" ;rr ""''8 q :," ";* ^tner r"-"'iiffi. ";L'.." - ri rP, "e" "a"c" n g"n .nFna I ou, soF.res of fire ants h' .t on .no! If.T rli orsmls\Fo tn-.l aiong mrnd. tb€ Yaard Va.n rass larners ln rhrt arpa sllng cloggrng and rng l'leslock rrches. resulrei praws and rracrors rhe area ts i'i'r!" barr,.,rarrysu-sceplibrerunre i"iiii ; ; ; ; ' ; : ; ; ; i ; ""i' q rnlers T h e Y a a : d \ a r { r : n o : d n o n l s b e c a u s Fo l r l ( m r l d of The rvprcal anr colonv averases -^gi.-"ratron t'i " ".i'ilt. eiectrical betseen 100,000 and 500 000 ,-i"',,i"ii.-'-"i"t'i"a an[s. A meral Prone l r G n l ' ] Re!edrCierS (urtO|J\ Lr. antn.l, an.i.c:r, oe wirile Benson deals with hrs 'i 'e "i tEi "."{ - t ;t;n. 'e! gna' """"nt ,a- . ". osourn . & . e'PaIcl m:"Fsnrn rlen:ron -:rl .hc l S DpDartrPn: of La-s-n. lhe ants lo Iu-r n E irr^' A F ' c u l t J r e t r ^ $ ' 'i.' ri="',e, "F n._r """L,rn;ir'e r'::!:o r!.:h. e:li ants Destrovi hou.s. the l,rrhin se!e.al anrhill becomes rls o*n ceme terv arre anis had so rnundaled bpn.or s I-,n, rn C"ntra: 1'x d. ne sats Inar n, .ventuallv earln i,o":o noi aro,o rn".nt. en homes. q a l :rc l s- xrn! a D'tle hrs acreaie ol ',ne anls - lher* p < ' lr r ' r n m u n r l : oe\-.op an BEn.or. I-memoLt('d lne €prode a'i. i:l€ *aler *ell and beEan ." rnvEsl!6ate'.ie Hrdden amona co.nmeal pel' lets developed b) sclenlrsis at Departmeni Agricullure lhe that are .luvenrle hormones r€lard the development of the a n l s o f T s p r r n gt h ' l a r v a e SooRaman Srpte BerbPr'cn saro tne hutronp slurs doqn all actvitl rn lhe colon) - killtng l)'. emarnrnsants sard research tn Berbenci thF R'lirnF of Ins"ct:- l\'oLgh Ji- o: hormones rs lr!e lr lu H€ toid son Rob€rt tsenson, o\Iner ofTrmebase lnc rnBrv_ an of h.< frndrnes and Yaard \!r^rr,..e-'-L;-. thal "Scren"rsLs ar€ findrng hormones in ins€cls are rn som€ sev'n.l u!l'iP inosr rn man Tnar. d' h":a.d mlr: veioPing chemicals lhat can drs'JDl tne(t srgnals 8P-oe'rch s. C !h" screatrsls hope ants q'ill nol d€veiop a torerance to hormones. as rne)' r'a\-ulLl- pcsr:.lce5uPnsol' rs Jln MFdi*n'iP exrensror' s'.relchrng srln!,it cords across llis )afd !o frd t1 01 the Pesls l: : a lre n- 'a o I'l rlo' : tna ' l . .r.1. ;, r-o a 'n of ants ;ii;";;;.;"-;'4.'';;;;^,. Pos\ible Datent R,r,.i g-nsc- !.rc lh" I S Paren: Office $,ll decide ibe s lului-e Because !h€ )nleii)oi n(: v€i oI.(i.a,.] rpf(re.po url.l lrP l-rce hoo. eti eerrso" bai caurroned hts father not to €lab.rale o. ite }aar(l Vark s \vorkrnes i: rnr Ber-:on Li r :i!r: r a : .n: R. b, rr hpr,s n L,-. ir ra-/e( rne -r:-rrr- ]'earsold rtsed hor"ones ;.I:irrj. ie. ;-:' .:':'-.t!l.x'=i " ! ' . ' . ' td te o xtD t-t 6 , F-. ha. H ,) o H u) F+ € o Ft a ! r{ 'a H ,) te 7i ! e-! ;gi;i lEilliiiEl,l?Ilii'ill lgEiiliEs eiiri liiaai #*l:iEi;iii ;:E:ig Eii; q:E+ o a ti? l:o l't o AC p H t) i; ' ^ :c - q - r P.fi bj3 ==' -rbr 6 - a l ! ^ . - Jq=--? E a - r E j .I : " 9 6 'c.: =.r e: * 6'3 ? 33 A E! F -i=e ;i€ iE l*i +*=A F € ; E i * = = F ' - , . 1rF; *; ? F i a inE t t f s E g g2 . = = * e 7i?E E x 1 i eg 1 E d27,2^*a i * ! ; i t 3 1 Ii +a 3i; Ea*q;F* 6 ' E = F a C e 9 ! . 8 tri; Ii* ;E= E i[tc e e l : E E ' ,: F'5=d =.i G " * F-=5 i-:. 1=1 * 5 P , E ' r F ,dJ ; i+ltgFi?;a'rlaie r;ae 3 z-t z t'+ *i€a; *; iiliie;i;iiin-iFa o ?:-*V;ai E*'Ei;a*ii=?; o * ct o { tD id o F' z' (.] ? CC€CTCO. CVCTL,RE BlbllograPht- Booka Tk"-c.! L. t{AA6qLgr^/ Bakcr, Jcrryr TaIk to lour Plante- Put e Ll8htlnlng four Gardcn, Fgs. 6lt&5,Nash Publ ., 9 9 7 3 . Callahan, f4flfp S.r l\rnlng lnto Nature- [t PSe. 195-205, Devln-Adalr, t975, Bodt tn Fllcntl,thc Ncttlc, Lenetton,Sellnr Electrlclty l n A g r l c u l t u r e and Eortlcu1ture, - Van Noetrantt , 1904. L u n d , E . J . r B l o e l e c t r l c F l e l d s a n d G r o r t h t Unll'ersltt of Teras, 194?. EflIerr E.C.r Plant Phystology- Effects of Electrlclty c r o p P r o d u c t l o n , p g s , 1 0 8 3 - 8 5 , l l c G r a w8 1 1 1 , 1 9 3 8 . on Ferengl, Eleanorr Green Thoughts- Conpost, p8. 4J, Vlntage Books. 1981. P9yel]r Bettyr ltl e Avant Gardener- Current Experlnentsrpg. 174, Houghton H1[t1!n, 19?5. Sheppard, Asher R., and Elsenbud, t'lerrllr Blologloal Effecte of Electrlc and Magnetlc Fle1ds of E'xtrenelt Lor FrequencyElectr1c Fleld Effects on Plant Llfe, pgs, 8-6&8-?, llex york Unlv. Press, l9??. Smyser, Stevet Ttre Encyclopedla of Organlc Gardenlng-El_ectroculture, pgs. )42&,43. Rodale press, 1pl8. G . E . r C y c l o p e d l a o f A m e D l c a nA g r l c u l t u r e V o 1 . 2 , L . E . !tgl", Balley- Effects of Electrlclty on Plantsr pgs. JO-J, ltacnlllan, 1910. Stone, C.H.r Standard Cyclopedla of Hortlcultureculture, pg. 1109, l,lacnlIlan, 194?. Electro- Tonpklns, Peter, and. Blrd, Chrlstopher3 The Secret Llfe of Plants- Plants and ELectromagnetlsm, pge, t63-?? and Force Flelds. Eunans, and Plants, pgs. 1?8-99, Harper&Boa, t9ij, Unen, Iilartln A. r Understandlng LlghtlnlngEas LlAhtlnlnl Any Prectlcal Use?, pgs. f45-49, Bek Technlcal Fubl ., t9il. I \ \ I ?!!7T+7:iliiii?iE 1+7?zi1;:i!11 i ::t=.-' ?;; tii t1:i+i:Z.1 ?:;:t::ili;':ii':r ;?;1VV, ? t8 o tr, o !J_ 3 D f i t T i i ; : ? izi i i i ; r z'+;=r1l7'=r,t +ii;l ,l'i:" ? 1i7 rt7, :: i l;l at:+:; it!;1:,Ez"ii:22 =t1; € 7'=: tlti lztt ; +?;i i i T i a u i l i ; t i. i iiliiirilli rliiliilirr '1 r t ' = = = 1 l - i a i ? ?=*P F . = : i = ' N r _ i - ; - i : " : : : i i i z ; if i i"zr\1:zt;':: i qt i. ; = - i i i r = ' l ' i i _ !1. g = - ? 7t?Z_i i i l - p' :i17i,1,\iiir= ? .aa :i at i'= 2i=:-i ," o 9 € r ! : r " : : - -z'|1:iZri"z ,-' "_-. 3:^a aav =r-d- fr'li-J, e(r.h u a i ; ?i ai:_;"= EVERYDAY SCTENCE AND Sky-5srapin$ IIECIIANICS N* E45 f or OCTOBER'1935 Torvers to 'Sfake Ilain O RAIN is caused, as everYone knows, bl'the upper air's becomrng too coro to hold an]'ionger the \r'ater l! contarns' 'ar' I'c Con"-Juenl,l, th, rdcathal rarn 10 maoe Ly nring Lomn' ir'r' th( :"1 shak€ loose the \tater, is inlpractlcal -^ French m€t€orologis", B€rnard Dubos. Droposes to produce rarn o!' l € n d i n g N a t u r € a h a n d ; i n o - t h € r\ r ' o r d s ' helpjng her to catry ou! her routrne Drocec" of drasing heal€d $at(r-Lau'n 'l a r r f r o n . t h e s u r J a c €o f t h e e a r l h . a r coo)ing it (bl €xpansion into thrnner atmosphere) until the water conres doNn again, His project, illustrated h€re, is !o Dul ut siufendous to\Lers of conrrele' $hich \ill cr'atc drrfLS iroilo* "irh:n in the same manner as a factorl'chrmair column rr'ill o"r:. Ttt" " " "u"P n a'i'n. tgh i t . a s v a p o r i a r d "* "hii..yt , n n" r , . ttn n " " N i l l d r s t r i l ' u l e i t i n a l l d r r e c : , c - n " .B S t h i s m e a n ' t r l D u h o s b e l r c v e s .t h e n c l u r a l n l o i s t u r e o I t h e alr can hc rPad ll increa'.d Such a int,"t. .," t r.enforce.l. and t\\o'thirds hieher than the Empirc State Bu;ldir3' { o u l d c o s t a L o u t $ 1 0 . 0 0 0 , 0 0 0 ,i t i s e s t i -ut"d. and be of gr€3t scientifrc as $'ell a s c l ! n L : 1 t i c! a l u e . STATEB'06 1,268Fi r.n .nsin€..s plsn to !lilizt t'i€n!i6c F'incipl'4 'a.l tor r'Frlriins 'rihct€' rrj (D a Ft Ft ffEEF alEiE t\ a ,f?f' \i \ ! I\\ I ffiiiisii )d D) \-, i , t ,l { v) \\ I E I D) z \r, l/' I (D U) 0 "-;iffi 1*iiiiiiiiiiiii' g{ 3 I \ S) 7r . ! I 6 a () ve\ io BI 4 i r 0q , r1 v) > IF ffiffiff lr i;iii;ie:iiin;i i; :lI o : lt' ,! A=) i;:l?F+39;;ii+s 1 L o 4 ='i ; o d € a Lzur l: "dV Fs &,J lflrnq, L.E.: !roehani sn of Nature_Z01(4926):13o5-6 (Mar. 28, f964) Plant-Cell Damaee in an Electeostatlc Field El eetrleal stlrnulation of plant growth proiluces increased , r'1nor etements (Fe, Zn, Al) ln active concent,rat{ons of Comparedto eontrols, are of such inereases I oef tips. tho order of several hundred per cent. This ls due to an tnel"ease ln metabol ictrenzvmes in the etctricallv-activatecl oliqoplants, whlch apPcar greener because ozone generatecl by electrl n d e e p - g r e e n , o p en - f{ ol6 nricllzes porphyrins, resultlns icel ' . Jn c e d n o l e c u l e s o f t h e s e o r s i n i l a r Under netallo-enzymes. the act lvity fleld, t h a l n f l u e n c e o f a n e x ec s s i v e e l e t r l c a l to such an extent that oe thpse srrbstances is accclerated cleterioratcell i s lmpeded, rrlth resultar{i r ' : o ll u l ' r - . r e s p i r a t i o n t l s s u e d e s t r u c t l o n . and I on Fruit TreesFed From Bottle a NITRATIS ii" i;;;il;;" gor tlE are tonics to a tree; it absorbsthem geedily lrom around its roots' But nitrates are so soluble-that nrl..l. they wash leadily out of tbe soil. A method of applyrng them. developed in France hv Dr. Moklzecki, gets 10074 results; the solution, onder pressure from the boftle' ls fed into the sensitlve layer of the tre€ betweel vrood ano bark, just as a Physrc)ad makes a hYPodermic rnjec_ (Conlinuzd on Page 880) it .daani.t.t'd. J O J5 l | ! : - - 3j; 0c I 8,d- P 66 ' A ; ? 6 Y ii ) + l l o r lt(D .t "la L o = t^'" a<l ! + x i 5 d . , N o .oa .< \ P = (-0q xxti n!, l ) : J .: :l S - ! ^ :^ ' co Fl - : idi Eo i x at ' ; ' i it o 6 2. g 1 3 X : 1fcF ==i' -0a (! | ; o , ' rr l9 { { ; F < H l9t {t = I= + i=^ < ' irl vEl .?t s,d E 6 o = ! 3 . 9E l o ( ! i9E' :i> o =F 6 O o c ' + E €3 UT<: . srP I i t -ii r , . 5 =(tcl ooQ ;:^ O ; *i- o !,P 6 e > L,iJ t !e ; : c- i6 iriliei#iiii+Tc *igrug$gi#ry giE srft*;F; F!ei; i )i' l l o ) 6 O ?a t r o. N l) r )o s i o li € g r iFiiFiEiFiIg EgE : x i x : = ; -'r i !-c #i*iiFnili +i$ i'r fiE (D-!ta 6 { Scio t ' Fn I Q s. i! r':t (ltl { t : P 3 i v l-l -. 1 ^ ; a i a z .- ! oo t ( ^ i ! ! Er Y ' t: € c 5 e de =,= ? vo v , , 4$ \ o csA .3 -ttj F q s +s !ar co 7 +t\ rt'{ o < (O c * 6*6 -C .!-D 3i. '.{ t z J al ! a i d wJ. d ; ' tF i :o < ! . + E= ' . a-- 7! o i rO .a; r:ii .. s i i ;q q l r:. ; = ' - i J o 'Ft -5i. < . 1- ' c .l (- .r5 *9' 35 m 1 o € o o i E t croq i .i r F ' d c I !d' ^ x = x,: ;v. I 6 o3' F0ct ; r.k : ;it) i c.r d t , q \ ^ t F l (nn 5 it c l Irl \ t n G \ a - ' E 3.0q : r et' 'lJ 3 T t x . r'9 - ' <:H; a 6'I 9 ; 2 3 .' e d ' l I g .a6 r > ! o - o c 5 tsr ',. ; l.r 8 i3" ix r.{ z q r - t i-i E - ' . 2 8- ) 5 ^'|< 6{ r Fi rcl Z'rdi a- X i ( 6 : 3 : : j ; ; 5 o t * l 3.x i! id i; t a s .o! ! t ! ^ i . 6d ; 7 6 5i r i R! PI . ' t( ^-itdCqq o i; J i ==- -x. \ 6 6 aa i i ct r ; e t d T P *r; i ' : ' E ':. j :a @ r d if + t 6 ?, .69 1 ) ' o o ,+. A : . = -5,3"i: --> 8Tt t, o r a '.| C, t 7 v I tF , .-J .-, " 7f \-rsrI7E^I_I,IlrF_,_ r rkr tEB 24 1926 UIilMD STATIS ()FAGRICUruRl DIPARTIIINT r37e@ EIECTROCUIN'RE BvLrrev''iiin'ri;5t.y,Orrr,,\"N;Iff b",;,;,,!,:A;F,;,N,t,once CDNTENTS u,"iflffip"tr"f *0i,"'iHi",l;iti ; i -Lis"er. 2 t$'ffi-m PaSo tl i,p""riiiii'",ui t7 l7 2 Ei$ljffiil#"':1.Jry[i*n;l*:*,"r, !l til 13 , The term,'electroculture,,ar x,::i ti::; Itrj-, *"ii;"iTffiiij$#:", irj:T-r #.{ilitji}liF':U;*i#.f i"'[ ;U"rffililig.xT-'#T;"'"i-T;""'i'".i"';i'l'f ine]ncr roculrure have o"#lXfi Ji""3Il,1il;"*,[t:xperiD)ents pr me n r s i" ai",i"*, r,,.ri r,ui'i i""lfiqli' "i:F"fl'J,;"3X?il1, f ;: "l;:'#ii{,] l;'i,'.T:i, ;,ouoJ}."li]ir""rl**'"",:-,,,"'.,*; [,,*ft:ii" ji",iiiill;ir t' "ood*t"d-L-v',f, a 1'f ,'llilfil" Jl""i.,n.iy."-o .ii.q-3l",,t " oin"" ort e d r gffi ^,i i"r"r r X g I:i i.e J, "" "" *,.ii-:lf[" :gf fi.I :m::li"*"-iul};l*:l1r#tffJ,:.'llL_*iri;:g:t .r"i :l.,ld ,l ther?riewof theliierdturcA aAt -"ririF.,.ifr" :liiLli'il;'-1".i'ff"b"i""1i:'itg;11{p;j1xfl :;: ;i;:'fl NoRMALELECTRTCAL ^ !n:^ft: stlt? Ol ?+E ATMospEERE efrectof usinga chargrd;ctr,or* over gro.ring plantars ixT,"j"i{_*",.#jlrJtr l*::*Ti+:{iil',;ti.x.rfl:hiffi*f tn_eatmosphprcand 1be cbangesp?od,lqed .{-o cxamrnalionof the eiectri"cat.olgtfoqpUv rhe chargednetwork. in tbe atmosphcreorer an_oppn fietd on.a cl_earday shorr.thrt th."" i. ; i;;; i;l; ';f, chqrgdbooJ'oo-ar".o ; rn orher*-a"lg,fl :,"":d_"i,qtxi:if f'"""lii' &^:'.ii:ff i erecrncal i::i:X$:lif reld of forceilsi:vf&fr "g_,i1,H:n""|fl isldentieal vit& ritr t, wni"n *"lrli urlii.,r ,uq earth were cbarged negatively. ' PbFtcbt, Bu'.su or 6bIa.ro", looloo. 62rie'-261-t , 1 , i . 'drlice.-, ar l!dl€d 3 ! |. l##lr isH#ffiiruffi g ffi fiffi#i 1 Fli ii$ Fni tri;; ;.;iiE E g?i iiiieiiiii,iF$rgil*1: :;* itiif s ii;ii*;i;lig ril*futg si*i*rxi,iiiLrli a ; € i z ts F FI 2 a c -l ;.?t!i=2 -i=:4 , - ::!' t?+:=3:{1t:11j : ;?1:a1:: ==ilt+E 1=, z 1 ;,'11 lal =il 1'=i1=_=" : , = ==: ! i = Z ! z = ?i = : tli : j=-=: =-=i= 7 j i.E = ==i '=t 7 .;t ===:= r3l; i:-i: .': ):1.== a' =Z- tn iiiE 1zi=_=:l,=::+L1:i ;zt i;j=+; -;iitl7tl;*=-i;ii a:a= =: ='* "-i7{72 illi *la:;11i'+i;: + 7 = ? ? : z 3 'l = = l = t " cl 1 * i = 7 = li-1 l -.t*! = t$lii:.? 11=' ++ .*ri i-31;B ?i-; i. :i*E I;:iE:ie.=: +1?iii = P"tr*6 2 6f ;< --' ad*:x;? fi?iiiii a63; eFF: CE I Ltr'F 77,=;i?71= i*F=:! 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I c^*L-- is l z E3 ii argcas;: ll G€. iisEsi;laasli 3iffii:;i; i 3l ;1i;;'?; - - . -- - - ir ii;irt;iiir d + ' d 5 G ^ " ao - =r. i+i11;3iEIi " b : -r E . a ? - ! l = r E:. :.-., iY :. !! ' ; 1 L i. a = : - :- a 2 e A 2 ;. +=t;Erilv o t ir :; o E * + ' == 8 . = i 3 , ;l :r t =i i: ''. Z)" -a ='," a =; li ! =- : i' -i 'z . , 1 , !7"i 4 "-==.. 1t ?L?:.iili ei = ' e . ' + 4 7 ? ' ;-. i : 5 - <- c r ! = ; = 3 a ' - - e Z ' ! 3 . .t = ; -+tr *)-:4: i aF +p d ', E r A ;I r Es 1 : i E+isFiill t q 9 i ' < I e-: e - tr=di $Fiiliffiiiiilffiilffi Tiiui * iiiirii*r*i1f ii ii :i ii iiiffiiii #i i! F] 9-= JJ a: z =tr o €:.c' o -- -; !, -46 = 9: P*':.Q c € tsi * 7>F 5 x * r 5 z € E; X a ' *";- F _- =.1. ...7=.- a x:-;; s33 ( < : : - ^ = - F i ; : : - r. 9 ; J rl90'?*g:+! =i i?:?a1?ea. 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E C:TgEFX:-3.A> !o iFa;I-: ii+g;si{i tii +tnalaB*iF-4lr i.Eu=: i i;Hipr;F#igii i l 3.r it FFE;5*g;it li li i =f:aa1zi:: 6 a : = l 6 - = . 6s l r l r l l z E ? 1= 1 2E nI ia c 1 : 7 le iil-=Ft3;iri F . ;3 s l ; 3 ; i : a . t A - * eT d: - E = ;-:q F L-ozEr l . : ; E . 3 ! i E r! = = = ; i > ^ l i - - . + 3= i : i - i 3 ? ? i 3 = - J : - . i: i !'; 5 =.. i _ a T =.' 1 ! - a ? *-. i a' 7 a t ; - ; = i = a ; i i l+: ', =_al.,:1:: != !i 1: i = r c a +i=-t f, l: - _:t. 342 _c 1r 1i.. : = , '=i77:+ *:1i-:: ; ! , ; : ' : - - c ? i f < FSl;?, z;itll - = 7 : . a D ^ = 3 9:i5 : g!; o ! 6 -.! -VF- e ! I | 5 a t i t l o =-*<- * n =Z =:. :^ -s c'=r: 3.-.-; I iR. 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J = l- :3 F33-z1Fg€ $+i ?Zi*8",3: El' ir j:j: :tr! f l l i B.rE ie I t g B si -t gF+ F !'E-;.8-. e - : i a e.t T E a ag - -'w: i -= :F Fls il. 6, i.i f E*;r1$ng;r zzzzzz,> c9?o99t - : 3 i.':.!d di I =j i$ r+[Fiu siEistiiiF5 ;-4 == +r --.'i. -d i; r1 :! i: = ' 633 ^i : : x - {!. ; F -r : : S* :- 1 8R,? z i; - I ll- ,t- t= -i *d l ' l -a ! : : ?J 5 ;- ? .E )- Fs9 : A P roi :-.. ^a : i =.1 ::d' ;'9 ri l= t a H E.g l e fFii $l#trF iii $ rFitiig h t;l ;3fe:"Fa e ; z E *< E l s o *irtuueg i) a l E_ 6 !! irI E59Ei*- c r < ! sE E F g - -e,E'Fi.r= F. E =r f:"ti€ rF :=?+7;7!=11t=-i7!:1=: z=i+?i{#=,=.:!*,,==tt !tlVi*l:;=*::*.:E:!:i=: € ' l F - :*217:-=;1t1',:E!;7t1 #i1t#*i*rrtl#:*#!#t**r D t t r H < z .l ; " :s+ciifriici asl *9 A;Icl cte66 gEr!E -ieE s lE:"ri lginrei;ti#e: - -a":E; i9-!: iE lei ai:E 3 >+ + I I z -*t CEa.ai=-in a;i; ii=.+3:. 1it:1 " ,rE: i; 6i ' E z g Q a F . 4: : 'Ei;a -d?; li a=;ille: l-;3! [Eg cp :i 5;:15;ai*i;s5l- srFiE a ;frf;Fi F3:rl i;ti5 g ;: 5 9 8. I t! Ft !<9:E.<- 3r1" -E5oo e 63.' il:! *giri fierq r i;*it:xigiFil: "+::i !_rlis _:;i* 3 -" ^+g :t ! : o'$; J 3 z ,l c 'l E e l i i : .i i ! i ' " = o iili :itili i*i! I rrlii: ii+ i+rt - 'Ei:-9 $ g :: 2l7:' -:; t 6 1 E t e g 6 aa z;qiB c - H -I i l t----l.,-. I . =i:= i !; : + 1t7=t:_=. :li:;tlttaiiiiiilii +jfii -i;;ij, r*;r*l: ;ri= =?i!:?:lzii ===i1lr;i ri==='1i:iii+ sli:iii;iiz'tz?lzt:z= ,1',1i;-': : =:: l. i== =++ ; ;ti-l#, i: i,= #ii+ _ili;: :l ri:ti: :;1: -i'l' :l-ii;==f# @ ? ) : T t : j - 1 1 a 1= = = :^ = = = q : i r + l : ; : l . l : i i r 7 i =i 1 . , L i ' i + 7 1 : - ==:= : : - a i1:F=-: ,z "l l=-; i= __= 3: I il:_= =_: : ? Z : : i a :i . = - i = E _- != ?: _ i. =:=_i -" -i 1 , . _ - =.1 a : -== ='=.= ?1!==li,j77. a .J ii1=l;;=7 ;="., 7=7,f ; =.= - - - =: : ; - ' a - . - = : = =i 7 =:,2 =----'i : 7.-=; = . = - . ' :1 . . , ; - t = =zi ? = : == -i: =- \ L 4 -_' 3i : g i : : lz:==^11 ==4: : = 1 :: s;=:: s ;'--.,i 2 -Z :?: 4 = _3 . --?7 ;t:- 7 = n ? = : = - = : l - . t a - !.= ' z tn <iri 3 . E >:3, " ': 'o-* : - : P 9 * -ag : = ! 5" f tirs L't j'< ! --* s3* *3= -iT E;: 19; 59P li: :El 7;L F;.' Ei5. ?a: 1 EF ? = ? 7-ZZZCZ7,zZ' , t i l =?= ' :Z 1i. i.1!':1:Ii;==:::?12;; - =;=:?:i . = ' = = 1- . - i ) - - 'I : =:-i;1i=:;:'=Zi+2-= iil.r:7:t7== ,:=;t#;: - : = = ; = ! : =: , !.'. l', Z=-=-=. : : : =^t: r-l - =? i : = - ;. ; =i:_7<1 ii : ir;:;;:1; li t:ti= 'iiiili;:l;,==i* .=t==,: = a1::,1V1:r:Z= i" J6,i 'j : i lir=i_111i!:iv1:i iil.i;1==1;t=t:='z ii i;;ii i;:;ll .=liila :aa;i E r . > t t j i i==1_1ij 1:'! 9i.:: ! = ! I 4- ! + >- -:it , -l =l :i / .- i:=' - / :- : i. l: = i . # t9 # / :' -i '?i ": "^ =! vl F= 3i i -j = - o: ;- :: .Jt 1 - .3- : ::i:Iiii=.:Ti;=i !3=;r .: - i- :: zz =: . =- =' -< : ; -: V ^ - t a - _. =.;=;.;:=ij]'arr."r- ::8i11:iz-;ii? {;*{it==ffi#i _ I = =- . -=. 'i;i i . -, : i ! s r f _ ==.== ; 4 , a:, = .z _ = ;_1- :. = : :. t = =i , + - - -: - i : = . a :a i: := - l = _ :' _ __ _ . : ; ' : - _ :.: :":- +a =-. ; ::.=:--! ; ) --j i i : tl ; r : . _ i )ti ; ; 4 - = 1 = : ' i -lq =- a = te -.: 7; =-. d5-,::.:-:_.+-.-l-=j i c i - ?l T : - =33i 3 = , = - z I;t =l*i.:=:':iiil* =i*==_ ::=ra|il? F# l==:i:1= -,., f:*=i#if *; , -i=i,i F i - j t j : - = - - t : = = : = _ = * 14 .- * *3: - =: == ai ; ,7:9 l t - tr , ; ' i : ; i ?! : - : -=: . ; ! l ;-r : - ,q. -{l', .l F S:: " ; :'g!t.-_iii:_fi-?ri{ " ; r i i =7i !i .1 = .;: ! , ;i E , = '=3 , " . 7 = != 7: +:-i -i=iq 3+{:: l: =a =1t1:=E?=?=="=.^ d I d 2 s:: + :-? o : : : = l - - -;:- r= = c -; < =; : - , = ; . V==' :I l i : ; E j ; .? ;- !; \=1= e"-1- [' ;=i=;5: 3+= ;s i : t i --c.a--i =? - - c;' = { ^ 7 : : ' - ' a a , ' = ;l:1i?i i*,"*pl1fr=;i:?*li1?1 E3F#i a -= =; _ 1< =?' :- X"r7Q -' t1rt e- 'h;i .ia lt:; g < t ' ; 6 < F r . : . ; - o l i o Y t . ' x 3 6 ? - ^ 3 = a 4 9 - , : < g - r !? i D : - " it "i i3. -=j * g . r T i : E t ; a-c=> E a 7 3 . & i " n -t -r' 3 *9:, ;* "'F 3 :L ,- ;J + - : : - : - - l. t=R. ; : : = a= -: !. r - 3i'<d iF=E"=frT" =_..; a 1 , n ; 2 I 5-5=-=A=. 4 = c b l o = = 6 = = = O a @ : ' r - - - a ^ =i=1= Ei:*Is.*?o 1 --5f ? ! t-i-d i' 3AFFF51 lr*l ri x :o r! ,: ? += >"Fd =; 5qr:H -.4! P iE:- o a a e i P | *E!; = ?. a?=iqi-'E+='! agl*;:l$*z -a=7=+ 3 ' 3 : a Et E F ! : . : l " i ir a.9ilEr*isi€3 = P 5-?t - r=x,T*!'ii7=1? ;;rEiir Fe:Fr€i it## i;;il*a!1?xtit ,ilffii * #Ei?=,rii;51i;n]r; i HT+i?i#1# i#*Fi F*EtE+ E=#?i F;a iFi*;*ttrt€H -t/* V iaiisjEii =5.lP'ra iL*lE"ifiaa;.?E' : 5 F D 3 J l = ' -^> !6 ;;F3p fi=€3=E-iF r i : F* 1 e ['?o3$3 B.sF--*: +; e 3 *'i ?'< + * ' s . qi : I il*E3ii:. t9 J-------l , { € ii;ii; ;gi+[i3fa;l*ir;q !:iii#1ia+?isg cE^:.a^ 3 a-&:-, :.i .-.! 9; >-- i = ; t€#i#i'|l+iiiiiir+ Hf##$iii$ .;riiii::iiiit ii*: :i;ri=;il*rei+E ; r-ii ii*g;+:*i;ia i;i#i*i=;fi : 3'd'r=; sSx=-- : - - 6 : 6 *: -d :: ;l ' : i ;: d @ +: -i :- :: :'g ;: ia lEisFiii+iiiiSii €i ##+igi#ir ; i*i;;;i++;'I+? :?:l= #;til:iiiii+a# i = i+:ti#ili: ;:i'iri, ;3iru+Fis's+sii ;i = ,rr'. =, z. +-: -1 Yo - . -i i ; €a :e l_e F tr<: ii.= P - *'< a. -. l - = (c> -ci . 2 / i :-; j-2: a 11 3T-; J +o * 3 3 l C^ i1=?5 "J : !-^P >< = x": rD i'=29 - -3- =6t =l ::a i d-;d!. :9! r?E 9-?? ! 33 : :Ejri ; 6 * e e F +9- 3 2-aa6a 'e : : c i C s's33 fi o Er;: - :ef 3;A ic 3r,=-= P- :^ o =!;i: -{'=!: <:-: - =:!* :eal *ell 3::-. 6: !n <--r ;fir -.3. : :=: i : : , t.:--=n 3 Fqe=z = : c * ,3 !.< -:--- a zz = ' - ; F iI ii7:i=E?=!?= .cl ?: r =;fu : ^ i . : a 3?==_.'' ii+#: ffii: ii+i#:;:il s;-iri;eiaag I r#ie iffi$ r ti 6 ; J; E e g; i i 6 E 7 ' = : E E i 1 ; : i:=it::;li t d: Fi :ir F1 :: . -l I r : f : iI $! R ] .:g: si s' i i i i # i i# i inlt i ;l g tu VI=x'17* 1; *-!ffi$i$ #iaryiiil t t9 e I n r-!. .t z i 3 -c.f i = i =t:;+ :tltl*titii: =li*ii#* J *t+:*{:i#{',{r=E*r i i*i-f-ii-r +**iiiriii z €5 '.ri T>Tlg:: 1 = !==2 +:-{i+1-] a=={ .e.:7v? t ftiii;ti;:a ii;=?t#ii " : i " : + c - : =.: ^ 1 = - = . : a-- i; sii E:1 =n.i i -+i 6 :, - . a'J ii ,a==;t{-*;7; E = = 1. ;--ia: I !-; !: iitiil;; ffiig a iJ &nk ."o El! E' r o3: J! i E i = =" < 3 d l. i: n=3 =: SPF c';le F-*; - : * i^- i:J - ^ 5 +r frt <2 :eil b 3 s rJ i.:.|]!.:.:.|-:-!P|-1.|-:.F|):..:- a3:5d3 de36!r 11ri 3. 341:3t d t t !FF 88ts 3 8 8 E1 + # ; 5k3E* FFF FEF 8t8 5aa E I : :> I . . . . 9 t s t s a a a aE t s t t s s g Bc a s S +ir*inii* I a F F F P F FF T 9 E 9 F3 . 8 F 3FF =- 5 19-fl F; 83583A a38385 5a5:53 : vY=4 E-t,-!* E4i'-= ^ r i ; - o o E==E:6 : 7 -=-' *'< :i e F595!! x a-:1i.i d I i il E#i$;i g : riffFFll€ i9 €,isFr3: rF- ;.sF€;tltg i i+gi#gi:;*tri; ifri *i+t+iii*iqii* *rrti!7tt;! ii iiF?ir;ii:lii i 'i -;i:iiii=i $ulitti# i' s+lligirl*iiii =g.* *li e.3==i=1; giE?3ri i3 j,g fii=S, = e1 i ti;iiiiii liffiiiiii : i€F::rtir - = ! =^"3 I i€= c i n : ; ^ - ' i: -: :_ E e'rTfi = 3 + ;i ; i f " ? - = 5i=i=r'li; =t rsli=:: : :r;1il ;;t- ;-!ra 2 = ^ + r .: :r;iliiiii l ! Ir i1==*E?1V?:l=r* i-z ;2 i ==-: ;=,li-?l=: lz I r I a i , : 3 r : , i . I i I ?. irli:li1:.zi .::aal=:; ii , i P , l l l F l : ;i ;E17q7 * : =:',3i17V.1 ri =i:?r"i^.;i +ii,;i5 ii:r alc a?*!:*i* iE':11i:1:l?;!;1 o Fi ! c c) e *:::3 B E:::8i3 i : 3 3o = E S E$ d i I i ? i i a - i i B : : : e i e i : : : d i i E g g g E $ F F $ e e 8i F S S Fr t S F ! F B PPI a 9 !.9. . !_. . r-r'- 9r!. I r e !! P 90a lDr' I . FF. r'f'!-. !-Ff_ ; s d F s s b g 3 r B N gI g a t r a 9 e s S r s a d & r a E 5 5 t s s i i 1 i ; i i ; a H r r l i Ec ; i.i' ri =: E;:: tia=i;*=c g:tr*;ii:iiie:5? E* i #i*#t'$ t9 _ir E:j=3= = z ! 3 =i , ; i=: j i'-==n= a 1 2 7 ? o? - , a ' i -a<' 7ii? ?<1" F i : e 7 ' i = : i a1 E 5 i ;g i! 'a- oi;E * : i : i i ?Ei E B+ ; a i ? 2 ; ?:,; l€ll!:ir=: 3>=-- :i i 4{'l:, -.: 1;F,7t=z::: aTcZ =dE- =63=i4':-r.i= ! E + 3 8i ! . ; - i I F = p -:i; E ii ali€Fg; Fii; =i;1;eE EsE ..-3i; ?sif3.3=--- a i a + ; l i Z l =- f: l -a^ '=j . =,D,ni? =-. t2 4 :5 =-f i ii.l z : ::-; : i " a = + ; = ='=. c A=? i 3=.V3 2 =-- Ir€;itiraiTtil _ 3 9 '3 ; ' i E;' _1 X: S .5 =-r : =! - ? st= ie,Ea lF ; i - V . : . =5 : ' 1 ^ € r " i * Fislg;Eq l3gl;cFFiE l i ! . = -; 3 3 e ; c t = ' i i - _ . 1 ' . c= - = 2 4 . . = L ? .o 3 1 ;.-6 =.q;=*3' = E:=?..? ' E: .e=1- a : j a d. -a -4i::-5' ;4, I c a;=.4-5 i:r = € * : ' f -' a.=Z t_= = -b 71 =) 3 r*18 :i EI !=**il g iii- lg -^--@ - - . Qi ' < ^ Z i e ; , t = i r +i E 3 : ^! 3.-=-:a:, _-":a-.+ i ililitirryl :_==_= =i+ ci=lii ? = . . < - , 4 l iTi I I 7?= 41. :: l3-i: i'i.-: ?i= - = 9.*:! Ei si*isi35;-*l "' :tIrI3'*;El ii+i U - tr *=;;'X t-\ 7fl=i=_ F r'-L' ; ;E-?:.3:. -Li gi : --;E:Z- ;; = 2 =" -= - +"=n+'3 af '4 - =< = = - - = a i- ^ 1 t 4 1 . ! 3|-.-LZ3jGz-g t - ; i : i - ' " ' - . . : : = d- <ii " i i : ? i ; = jd ;-" P =- a: a = = 1.rq- = i'-- 3,i :i .z = --*;. =77.5- e-Pf;a 69. d9'9 J tsB ts gcs 9. r :r d 3 a - 3 e, * 6i_ _=5 _ra: 37*7"tr: Ir e*i-E ; g ; g E l r ; I .. 1'>4 - ? == : ? - = = i ' = l -tr-===--==- ^ _ : - == = - ^ - _ - =- r - : : j -t ::" = 7 = < ^Ei53t _ ,A 1 = s :\ i - 6 - E: : ! = .3 :: : - ; - ?G --T-;l : - = - - 7; l - ; ! = - ' ' ,\ :-:.^E:-: , ;;L+ ; -;- . ! S :' : _ - = 4 +_-=4+ = ; - i + A . . ' . . )>- 5 ; F F ' ,' )t F 4v : = \" ,j 2 i -a i a c s 5t Fl : ! d t i : Z* + = t' jr'uzi;,+'j,l * ir;ii ,=( it: r _:, : j_+A . i.. :,a; r F ; .- e! =*=;i:l'iii ='*!?:i?= ; ?i i.'t'i r:Ie - " : = : - - i : . = t -l ! ,-1.< = --_=1 - spfs-p?6-i-i?6 i < P = 1.1--:;lz?) = - = ti = = : . t t i71?:?:a;i 3,!ii!=71 -;;- I 3 ' 9 * # r ! :L I cI ;g g :+ : ; ; € 1 t l 1 4 E . . ;i o- E I ' I *.'g*H;ii? :rH ! E ? t - :to; r. ?z r- . -. *:. a d + : 1 3 ! i * 1 iF E B r J 4 s 9 9 B 9 s s u ; e g E8 ; Bs I Be l ; "' a. / -. ":.5 &B I : s ; ; i SI F I I : : 6 J ; i ;IJ98dS53 = =.j-\ 1i1!;;!!a: i r I a.' I t;fsgl:g , i +;i?ri-ll ;2i€ _:Vr,13e;l::lcl 6 t L = _ " ! ; -=3-E 7 = 'a aV1l.:" a t == a .s b : _ f 0 : r ^ a:. t : t ! 9 ! gr[;[Fr ;iffF;s iiii*Ei+?,i ii:;i;1i:il;i - i #?lri-li:rir* i il:iiiriilriiiiitl ++*;l; i' ::;;i?i;;It::i irTifiiii;ti 81"+ tlii*=;=+1:+ 6 i q 4 FFi 't\ : iF;5 a- ';; l:: e: ; !1=^iz :i:;:i3 l'i=:?2 t r t : , 3 l i i l S .l i f 3 = = ; i l 5 € ' ; I l l F I E B It z a - 2i1i,3ie? lit nii=i [rr= = p _ . - . = =2 . i 7 c i : ? -_:;' E i ; s :i -, = = iiE 1P : : i t a i .? ; J j a a : ; - = ,== 4 1 F 'c a- ' - j t ; 5 : i ^ a ; H r. !17;t11tI;t =, )= : t t tr! EI 6 )=' .-l z \ .t--fd e : e l : ^ ' r o) i.:= o = l.g '{r { 1 tEi i 5 :- O r :-; =Q t.^ l 4+ 6 d It 8 l F Ej{ :) ii t d ilia;;r=,;t11 o - i - " -dj : ' 'iit tt ii.iii?i i'4:1i.-71 i===-11127;j7721!= o a = 1a i r.g i 3I !+| + o ' r.l < f F ,r11,:7^;:iii* -:=;Z;;iltl;E c ii*i Ii?E:lt ill*t*ui*;r t 3 3 G g E Ce j >ts -i:: q:i 'i : l ! : : ii i i* i l srlli'i *f*5 gi';cF' gi ;F'g *fl' ii='i' =';tiirii'*i;ir:* *ii';iu= ;;. ii:i ;; iiii:! i g$ .. ii ii:Ei ;ir 5 ;i=: i 3 3 . - ; e e e o e ii' i; i;;i=ii ;fi.ii:i'5; ii liitiitrEg i l l; l; t i i f ; }g ';;:i gFfe; ;F i9g rri # iF ; : i i* i; si i ; g ' ;Fi z 3 3 s 3 3 3* E F 3 x Cx S t 2 i 7 ; i l i : - - c f = i : - ^ ; 3 ; : j - . t. . + =i - : = 1 i f i ---l':- = i i:t= . =i ?7 E I :,= E :; " = i :' c e. ) ; ; : t i g i t a , _6 * 'L i1r1 ;i i 7t ::- FB ;i Z * i'.; =i i F ' iF =,:'.:;7==i :i t= 7:? - 1: + ;i i. i i ; i q"9 :;{ =; I i fi; - P s 1;i F' !o i? ; s e rrl 1* 15 4=z ;:: q ' F it T Att i: s ' s, F F : i Fi j'g -b: t F ! t + f ! Y - = 7 , > 6i 7.i = r = ; E E er e =' s:= F ei: a a:; F .^ ] F fp d* a ? , I ' E X ; 7 =i=- :le F=* i; u + n : : ;Fr' a: ; = ; " - ; l : r iF*ii: fi F ^ * =i c, E* ;i tr ?fi ?= P t? F.;i n ;i =;: 1.71 t7i'i7-; :E {:;=1 J:ti i =' - - ; i : ' =- . s a _ i ! = o . - . -r ,z' i!s - u r i = ; t j ' i ; ; = - = - , : =r , . a i 7 t9." ' -: e J.: i; - : - i - ' < < = -: l': --= ? i?=,2 i= ia ::l it Zl*- = i? li i+ - - a- 11 ' 1 -;a t;=1 -.= .= 1 , 1 = i : ' t = i = . : ;i1 : i7 I; ii = A i' :: 7 i o :: ti'i n= P 1 - t1 =r_ t=l j : . - i = ,;! . , ? = !<- ;41, 2t ."*z ] : I. ? .i+ :-i :ii Ii; = ?;. -il;:;i - t, -: ; l4 l : - s =. F :q ii-: $ =! ,1 i i ;3 Zi t- . :e. ' ;ii i : i i : e i :' =l 7l i = i ,..?_ ci, .= ? 2 : "" t " *-3 ' - . - 1 .Y * -v== ;:-- = ?.=i?r-* 9:?r" it ;:i r''" F:: = = i: fe ; : . ; = . - + E r l 7 ?E ts >5 i \ * -! - i i- := E ; *6 l<e- ; fF- -F L :- + F ; 6 € :*r :+ i7fE '-: -..F E; a !: ie +L ; u-.? iF F 3i. El i E F F F E: n:. iF a q 'A i iI - -=e,6? i + I + o o c F ,] I * r @ l < A a o T;: >.t i9: " -= ' E. ,'! (F 7.' 7A ;:- i_3 <6 -= :A ' : '9 ; > P . it 3 X 9 H E t J F!:- *ics : e.' t ? >5 r:- 5' 9:-E i !a <A 9f : I -a !; ' .:, i >: E; :-3 N6 v j .= I9 9-: e E6 3S *f: - -9 Pe 6' ? t - ' ! ; j )'Ei > - 6 o I 7 2 . t= ;: a z ?c gf cn{ :s sUE- ;l -9* ?E E;3 r1 6i< ?E ! 7* B 5 7 B B i i i $ ! i i i i i i i i i l : i r r i .i i i i - :i r FIRE ANT KILLER }LECTRONIC ZAPS ANTSDDAD r Electronics 1 , B o x1 4 3 TX 78140 i24-3373 E E.:' f=? i6i l-+ < d o d Fs FE: E9F :' !:d : .- !*E \ ? * 6',4 =g EE : i-ts \ f n v, : e :E - a pt 5 : r d 9 ol 2 7 " ca t = Frc .=- =: =r 2 f, 7 s46' :6 ; 6 F r1E =. { : R - :i !'< = o -c! - - i : o o .;B -: --T \' D -' "t : = -: c g H ;{ -; E E *g o x : X Sj t t * > F r.l E z ts c a C c c: c gsi_g_I!F_gs__9l9!9gg!_FFpq!s cF EnREMrx,y Lor K n - ni g , l t . t , . , w' o' schrnEnn developed tlreoretical prcdicLions of elertrcnngrretic rcsonance phc-rxxrenain tle cavity rrtrich is -*io e.rrLh and the ioncsphere. pEasurencnt-s ha!"e been carried out for several t-"* in tlrc frequerrry rclllge n€d.r f0 c?g. rt was possj-ble, for exanple, to rec)rd v!_1 Il*ft .,"o.ofy in the,U. s]-uno in Cernrany erectrcnragneti-c oscirrations riqhtning. ry signar"-,n er" observed in -caused ) the- frequencv lange qgb*"I 0.5 cps. ;; "Grq 20'.p". wtrich are due to field variaLions of local character. T?resigrnrs Lr.;ir.i"L"d arxt their effects obsenred. posr-tive resul- ts r+rereobtaiied- by reas'rerenG arproyi-ng garvanic skin response records ' The noultirrg of tte- Aptr-id *na ulter the influene of artificial electric fields. rt was pssibl. t" grcFrth rate of lactic.-acid bacteria and of yeast cultures. "riJ'& the yeast *rt"."" utri". t'|re effect of these fierds reach€d the sar= qrrchrth stase. after nine aays as -nt or cultures, rnt subjectecl to fi.elds, afLer 15 days. A list ':st ,*. -"G..*a '*iirr ti= investigaLian of trE gtrurth - of gerrninatinq wheat s€€ds. .tt , ore rneel of de\r€rcFEnt the _rt "pp.rtJ-dtii seeds subjected to electric tierai'greL', oriir= .,r,or"g., to a rength 23 per€rt grreater ttran tnat of seeds rihictr r.reie rrct sr:bjected t6 efectriclie-fAs. S"IIJDIES CN TI{E BIoICIAL TxTEIs oF GAsEcUs IoNs. A RE\[EI^T Krueger, A. p., lbtaka, S. and Ardriese, p. C. Speci-al l"brtrograph Series, 1966. I, eicneterorological Centre, Leiden The cx_rpri nent-al grrr-qram on bioloqical effects of gaseous ions onducted at tlre university of california during tlre p""i-t years is reviewed ard rerated to sirni lar r'ork done in other laboratories. " Acc.eptable evidence exi.sts tltat: 0.) (+) and (-) ions ac-c'elerate tie death rate of r"iciococ.i, a. coir, s. lrancncnrs and fungi. (2) (+) ions raise the blood level of 5_hydroxytryptanine (5fIT) in ard produc-ed generally injrnicar effects on tlre respir.to.y (-) ions t act. Trce Icrner t].e blod 1eve1 of 5fn ancl reverse the changes .uot"a (3) (+) ty'(+i ions. - ard (-) ions accelerate the grqvrth rate of higher plants. niy aiso hasten the on set of iron chlorosis in plants grcrn il an Fe-iree nrediun. lrrhen pe rs present, . they speed the'ptake of .,€gerDris Fe, i'crease the 02 0nsurption of seedlings, i-ncrease the @ntent of cytoch::ore" otrrui i*;#r"r;;;;., decrease tl* agLive Fe fraction, and increase the ".ra residr': t Fe traccion ,ir tfrli ptant tissue. ltds operirrental evidene has been utilized i-n developing an nfrtnesis or air -j4) .9. on plant the sites responsible for Fe distribution, .a"bgl when larvae 'or of the sil)<rrcrm are e4osed to hi;h @nc€ntrations (+) (_) ions the of either rate of qc*rti is accelerated, tlrere is a nrarked incease p.oat,"tion * tt of qt"F. peroxidase, anl to a lesser degree, catalase, ard tl* spiming rytoof silk "Irdi"", begins earlier. (5) The effects proarlcea ry .i; i;*-""4, w-ith the bio_ logical substrate arrl i-n scne cases with tire ara"g ina a.ni..r ilture or ue .rte phygiorogicar c*nnges can at rines r,=o# i',to p"tr'"r"gi""r ]on' states. (6) Daeri'Ents witr air iol" -*g difficulr to perfoim arrr ti b" dc,Erdclose atten{on to precrr.& intelference hy factors srrh as uii'poirutants "ifriii"-,t, ana stray electrical fields. *##'tiii}'r:,tl #ll*:p+''tr'ilig".1itr{;ifi r'zo,,thoa. s"s;.:!"i.1;t k;:r"t"',-',#:t?1, :-Noui'M'iid* lj$j;,;lT.illi";1,1".",,"":,".""1f;;'.T,oo'f rheembrvo j;in iirl'tiiil';'rr'J'TBli i "ff;jl?'il Lu.K"i"hun rrnsr.Fruir.Trees, FujianAgric-Acad.per-rp. Rep **l*,.o "r'lii t iil n"i,"ili-",i;:'iiloig *ii::"ili:,;,j.;"fj:,":i""frj1,,i"":,:d I fl#i;ilt"?,i:it'#"Jl"l'"1;l,l;ll;1,;[l:"1'": . uphle .nd the'K and i;;#t-"^,T l:?:,plenthishlyi';;;: s.! :Y,]:1, !-*q. which lhe rae of Ho ffii':ri":i;"1ffT;:"ll""lT,i ,* i io.s ot 5 Ar resufkd in a" iiti*tion of eoil P'a-nd K. thpbioavalabirilv reeurt. of P andK ws' incressed. Ar a 8eherdly hrgneet wrren rbe embrvo *rr *:riixt';*t H'r#jftT! ff ,FItrTJt5ffi!; j -ffi ?:.*tffiir'H:i'f'."H#'i!L?:[fi z K, 1c(, fttne Thc l'heory of Electro Culturc By llobcrt D. I! cCreery rvn hc)icvo in thc ionic thctrrv ot fl. I Electrol\lic Djssncirtion" on n " " n , , - $(} vinced c'f thc fact that rvhen cn" rekrt,ic c r r r r o r r (i . l r i r e r l l r r o u g h r D . l c c t r u l \ t , , t h ^ r c i r l , r o , l u c , d s . m " v c r l e r , tr f t l , c , , i o r i r . l ' th:rt c^rr)' chargcs of ctcctricit5. > l r r t l r r . u . e o f , l i l r ' l r . r r r r e r rt h t , n,S^tir, a i o r r sm o v e t o t h , t ' o i t i v n e l o c l r o J e : r r lrll . , f i positiYclJ (l,xrg.,l i,rrrsmov( ln thc Dt.rJh; tivc elcr:lroric. \\'hcn altcrnating curr!:,t is uscd tlis is not the c^sc; the ioDsrnolt 1 3 n i d t . \i' ; . : t i n o n c d i r ' , c t i o I rt o $ a r d s . r clectrode, then in the opposite dircclior, J t o \ \ a r ( 1 .i l r c o l l . c L T L . . r s r e s i i t t ( e r cr u 1 - -*v ^ o f lr' oscill:,rirt Ftrl'" of ir'l, ns. r il n,ti.rn nr.trllc, crlsod by, and in unisou \,iith, tlu. + u ar t s of iii' :.ltcrrtatiof curl{}lt. \ \ ' h , t t l r . r t l l t l ' 1 r ' r r ri f r r c r l i s c h e r qhcr 6 : . I -tJ frrqucn') o!,,lr',:1 lhroulh 3n a"r",l \oil t.' n-,ctrllicrlly coa1"d sced, froD: r ' l o c l r , r l c s / t ' : ' r r 1 ll l o c j l c I o t h e r ) , . r . : . h " d c l e r ti n i l i " , r r t l l ' f h e c : r r t h i . i r , t l , j s t a s q t h e e l e c t r o l j t e ," ; $ , i o r v h i c L ,h ! t l , , r . l i o n o f \ \ ' a t c r t, h e r , L s r r \ S / l)een glicous ions set, ftcc. lVhcn t|r clcctfi(it] is :r14,licd'tlcsc iols sct up I.:. . e 1 i \ _ eL ( " n l l r r r ( l r ,r(r t o n t l r c s c c d sr r n , lt i : r r o o t s o f l l : - J t . - i l e t a n d s i r r c ct h c s , r r ' 1 , and roots arc porous it rnust be oldtr,t frorn a rnccl,rricll stlrrtlpoint tllxt sorr:c ubrorplior, b] thc plxDt, of the gaseolrr iors t:rkc-qplrr..r. Altcr bcing rbsorbcdlr; tlre plrni roats thesc ions crc still subjrrl lo lhc iDlluc)1cc oI the high fr.qutn"i clc(tr'cilr rvlrich, during applicnlion, till set up librLrtiotrs within thc cclls of tho ptr.rt, Such :ru :rction n-ill meclr:nicall.r' cnl:rlr'. the cclls in rhe tissrresof tho pbnt arrd allorl it to gro\' morc frccly. 'l hc di"clrxrgc of high frequcrcy elcctricit\- through sc'il is rlso cquilalcnt to rorrti.,rl sin(1'it crrrsosrir to l)e dmNn into lhc s4il \ ith it. This is in a grcrt nl.a.'ure tlre principal bcncfit rlcrivcd from cultit:rtion ltnd is, thercforc, &n importrnl' fuclni in .gricultrnc hcr:ausccf its stinlulitiolt cf beclcli:rl acliorr' Ntorco.r'er the discirLrge r.rf higL frc{'rrnlrv etrrlri, itl ll'ru:rsh tlr. rrir c"rr t i ; " c . ' r r i r l ' r l ' " n r o i - t r r r ,i:n t h r ' " ' i l t ^ c nitric :rcid,lYhi.h coDteillsritrogfn Dro,lr.rt in a fornr rerdily xvail^blc as Pllrlt foodOn thc olhei hrnd t[e t'lectronic collisions of thc io:rs rvith rrlkrlics in thc ennh I)rodrr.c nitrilos. Thc cnrth in n scltsc it"".,t,t"" * stor.rxr of Dill ogcn il.!t m\Lstl'c ch:r,lccd lrv thc soil bri:tcrir bcfore it l,ecoircs avlil:,1'lc as plant food' Thi; rrrrkcs rrolk f{,r thc soil:buildinc l,rctcr;r' I n l , ) { 1 1 rI ',r ^ f . C . l i . S r " r , cu f t l r c \ l : l s j : ! - ] ' L , r s . l t j s ! : ' r c . \ ; r i r r r l lr r r l ( ' o l l , ; c .; ' r n t r - l I i ' , ; r s ,r i , . o l I , . ! 1 L : r l , . r ' r l i s r l , n r ; i r : e r ' l 1 l , 1' ' . : r f " s s 1 , . ' r l : so f s l x l i c c l . ' i r r r t l tl,iorrgL soil toltrinilg bnrttrir, {hcs' I o r : r , , r ' i . r r r 'li7r rd r r r i r r ' r c s s c r l t j 0 U f 'cr " r ' l ' 'l'hcrc i s n r ' n t l , " r i n { l u c r , , oo f c l c ' t r i : l - , ' ': {errncd be rrhich might o"-ifto-fr""i,"i,t trsclclii.l slinrulrrli(rD J',r(ici1 ore -'luq^'-,,.*,,,'i'rns arrd tL. g'lv.r ' ", ,i ri ,' r i , r"r' ori ' . t l r c lIrji Lo'lic' rq" r r l r ' ,r . r r , r r c" r ' , : r - rs t l , . : i l l l t i r i l r ' . i i : l r r t ' , i ' , ' r r u , . " ' 1 , . n u " nl e c t si n t . i n ' l ' i s i l : l l : r : l " , r r r , r i ' i l r cj l r t c r , o r l n n rrr:r1 - i l , : , 1; , i, , . , , i ' . ' j r , l ' irr,',,",'"'.,''' " " ' ' t ..ri:,.'i1,.ii' 1,".i,"..i,."n"'1" i,i,,1.,: ",o,'t;"a t'cbruarl'14,1920 El. rdt dArttftidn A! cdtrr! A xd i I ; t l ' 6rrla ., hfor Electricity lttlalild 6luhu in Ar Itr/ E brv. hrd d.i.n r t r. rJ' nbkdlv b .rDdib..L ud orh-r otr hr ai. oUw lada! .'{.J ot !|. d CoFicily d lh. l.6udd oa xr. tnilio old! aDnh oa pl..r. .l R.public. AiE, AItinltm B6d lt!, oa br. i. 6. U.lt d 8t t4 Fn! Lir .lrdto. io lb6 t-a dvi!. ra i. d.vCoFldl oa r Ftd rri<.I 'ni.[ ii ir F!.-d !o DrlF tr- oa .le Th. r.t' ld .Fi.tlft n.ldl litd tf,.t hi. dFi|ml! nmto' clrid !$tty oa d.n dmtr.rb ![. '$rldl rhtch G -d r r.dduF, Ib.la.nl. Fid. lbfr b t 6ur.d N I'o-a.ld. Ed rdv$q. ro !b. plott, | . diH! 'r.!d @l|!tr. 0l-6 rld Fdlr, luiru i.etul ha b bn o6*v.lid ' ld H &.ht d ud orhr ctr Fu i lddo(bnDd I l.'ldtto. L t'!*t Th. !..d d EC!.i.I I rhdr onlf b trr I ilt b Drr dE d I -!h ot !f,. dt6r., lo!. t-E 'itL Ill lbu.nt h.v. r. !. dbndod dlirLt - Lhc I t F rtdl.rlr |! .sl b tDrldE bll o.t M!. ol-! Ford r. I h. dFb br a[. rd d rbi6! r.bE, ire rb. ND dd b m6!.r.ddi!.llr l Th. L ddrt( in6k ..d o.hd leubr* ril dr oa th. olmn .yMi ir ddh.t:l r mh- | . i.{u6 rhioh n q i. hod6t.' Th.l dred nrf, ih. b...An b h. ddiv.d. .Dr' dt ol instrllrrion rn !n. .Finllins I @.1 du. ir nnEd .t ItO ro llm F b,rditrr 1o lodl ond ronr Tb. Blal Tb. ryrb6 l! Yrr nnple. 6y . botor r d-d ro . tult bt.' h.i.bt I * tution .n.|E th. Dpdy Err b.l rdl.l rtu ftiu Te hth de. a ur orhe sii.bL 'rt b. iEr.I}.d. lFn xhicL giDi I bF dF I bt b.d ol dibbl. s 0rFrd.d 6t'li. Tb. pF dpply .imlr r..hr I ridllY,l borzk 'bi6tr Mlh &b 6y rddiltl rd iv. b h c or b.d d 'ith .. .v.trly di'r.rrkd b. thur dppli.d .a 'rtd. d.r6 I b ui .!.rnlH ! Whsn h tr d.ird !!.1 nk. . luroir ir urd. ihb 'l'i.h di'l rk i. p!!Da, |. b. .uhsl'.ndt d .b.% indi6t tibubd in (n. beffi ol t. irco bnl @.n't ths Froi. rDd chErd Ced on rn in.ulrt€d dpp..t !l rrpltinr 0,5 .nl.F lnn . dy.^nr Th. ib! r.ll ol in. lrnkl ll0 voll,. pol.: !h. nq.tih rds Frri'. Pol. I qE rlll iuulrb{ -n&6 oa r mnF d6pt rh6 1ip. rr i. cl.in.d in.i a.Linl blo plrd in !h. xtbr' rh.nriql rurlo.. 'iib D.oduc.i.n Md lbii rt tho sod.. hrdF{€n p.turid. e b.ne l.meil of tn. rFxtu.k cli'n turlher l|lr ol*'' &in to th. tl.ntr ol th. lhrl rhil .ler.in6tion .lFri rould kod to purity it .nd nnde? -a! rdrptsd lo. d.itrktns Pu.r6 ir b..6 hr irt lled bi! .PPEiu Mi OIdn Aid sd Bu€nd I ir . Dl.rr'lioi lb.l hy r}. uF ol .ir ry.intling sd tbor6 .l r b€ithi o( 5 b.Ln rcl6 ol 3bu.d FUnd sD. d hlndr.d r,N qtb '€rY 'd lsrd lidcr cultje.iio' nr dF dli. TL€ .PFni L!.6.trt or ih. muli6iP.IiY tr ldr .dopt€d in -dd&iior r.d it Ais !u.nc livinS dlv. sd a.td€n. in ili d. ptrblio Fdd tha rt id lhd dun{ Mr. Oltn !rhict' tb€ Epublid dlbdt dreulhr lm in 1010, !. rg l&!d td ov6 ir nonlb! p.laud verr i.. NP oa lltrll. . rbl. lo fi. ond ed ro updy ud lrsiLbld BY 'it[ tdh toddd f,ot' d e ifi!.rion elnci.l oa r ryli.D ir!.llng 6l tlxir @P, lhd oE Fb6 Fl! .€!..i t!.n-lvd d.dd t .bl. |. bNE doushr, ldlinf t .t !p.n .nin.i.l "rink' rin l.il. suFl I& in 4 tt3idtotr irtdtjts t!. llcid.lrdly Electrification of Seeds.-'l'h.crc ap!cers to l)(, rnLrcll tDter{'st lu tLe cl(trili(irliou r)f srqls urrrl thc :tDI,li,.| tlon of cl(<tl.ir.ltI lo gr{)rrirrg l)lunts. ,\ ri.ccllt ;t.(,, rrj of \-olk llloDg th(.st, liIlos tells of !r ntrv mc )oil ()f aldjo;l lllant Aro$'th. 'fhc sceds, l0 or 20 slrcks, rrrt, {}hc.d i|l t lllis I'l.u\:ldN] \rith lron eloctrodcs ot l{, r e D d s ; t h e e l e ( . 1 r o t )t e i s t t - c o l u o D o f s o d i u n ) D i l r , r t , , ptrtlcularll. o! sorno othor fr.rtillzer. r$lth c0rr,als $ht.llt, barley rl)d oatri-the I,ldds of both gr:1i unrt stnl$ aro to lJe ilrcrersc(|. Sonrc afn frl.tD.rs lrr\-(, "-nid trlicn ul) tLe tr|'.rt'licDt of the secils, (hjch is firll,,sf,l l , I ' a y e r . j ( t i r e f u l d r t - . i r r g i t l a k i l r ) . , 1 . t r ct r , r l r t n r o D t j \ oplticrl lirout :r ntonth or t\i.o bcforc ao\rtDg. ColorcdGlassfor ScedGcrminatio:r (rltl.t sur.t,rr-t,r.l ( ) , . s L r t l st l . r \ , , L ! j r r , i'rfr.,l b\.;jrr,\r. ,; .,.,.d\ r,l,l,.r -j,..L.tj U of (lrlolt,rl gltiss. l.h, J)liur is (!rc \r)rjclr i s o s i l . \ ' f , , l l o \ \ C . l l r l . 1 t r r . ; - . a ) . . h t r f ,f o r i t srlri'lt (oll{isls of l)r.oi,!1ilr3 j},r, s,r,.1j $i l slr.ols of (1t.,r.{l flrs. (luril( {(.j" lljljrrlioll, nnll f,,r lr f(,,\. dir).i lliif, tl,i, l i L i l , , l . l i l l r i s l t j t , , , j . , r j r 0 r e s , . u r, (,111i. Ilxr'l- !t:rsj slli)rr(l r\jilt tlLf r,rrilj,..,.yrf n i ! l r e . , s , , l r l i l l r l t r t l . r s , i r _ \ \ . r j . :t l r , . r , i | . l)o!{r Icr'.r \\1,t1 ir,(l1r.r1. .j.1|lri lh.h j: r],, ltc!{l 1o gu 1o th{, (.\Do,,rr (): g,rrlirr:: r.{,;Ll ti')t,'il;llus,i. Strclrs of gl;rss ir rc dr,.jrc..l color rrrc I t,l,rrctt o\or. ttrc srr(l bi,d or. tirr,tro\, ., :rrr,t J ' r o o i l t c r r t ) . ( i i t l t l o l l t l | r , . l r ti s r ( ! l u j r . , r l . l r Nas fout)(i iD nn olitbor.lltc sor'il,s r)f (]\ lx.r') cDts thxt tl). scc(ls gel.lnitrlrtfrl l)ctit.r lrjrl gt,,\r; , ' r t , { l r \ ' r , J r , , ,r , 1. ' : - s , , , . t , o rs t , , , t , t l r . l \ ! 1 . , , t , : . r b l u c g h s s . l ' l i c l i t r l e ] ) ) l l r t ! s L . l r ' r r i n l } l 1 ,l . l r o i , , j - r . I i . l r 1\'cIe girnli CriicllJ' l1)C slt)lc trorlrjtcll il(l jlt.t itrl, sr re nunrLr'r of str\ls \rr.r(, allo c(l il] cJ.:h c)t:e._ S . / . , , , , . , r , , 1t i d Dt ; ' n . ? IlrlFttd r. ,rt |' +i6h tt ro rt.lltlta.d d *:ftir. ,Er Fd .ntndi L d'd.d ntr '' 6E ,r. rl u.d llm t tb.! rb nta Ei. I' t.L offi!! r. drrt tlE. 1l}. ro6 ud olhllllbdd DUtP6t-. lilv.nlioa FG I M.. OIE!. ud rh. I or Fi.!b. l,r. Nhli lb!.d Dl h! .born bucb Fr*vdc irr.nh 'orbor out ll. d.t nr o. hi. mrh.d l lL vr, ot ..'. dt-lEr. r|A 'dt r - - . odr.t- ,- tfl..tr.atc .dt_.'hbbrr, r. o.. *ld-.dtr.h*, t- tFq 6r.Fd. 1 l - a COPYRICHTO 1983 by r-o.um pres, Inc. Alt Righlr Res€Red. Electroculture of Tomato plants in a Commercial Hydroponics Greenhouse FRANK M. YAMAGUCHI (deceased),Uice.president. Research& Deuelopnlent,Generut Agriponics,Inc., 1272a7Saratoga Crceh Drirle, Sarotogo,Catifornia g5070 ALBERT P. KRUEGER, t,herit us professor,Department of Biomedtcol &-E-noironmental Hedlrh Sciences. School of public Heolth, Emeritus Lecturer in Medictne & ReseorchBiom'eteorot"ril, u",r";iir' carifornia. Be&eley, carfornia grz20 "r ABSTR,ACT ,' has gold h lne brupt )f ihe at the which rd the .An experiment was conducted io evatuare rh€ effecrs ot air Recently, Winton er d/. (unpublished data) at Oklahoma Slate Unrvcrslly expertmenLedby expostnggreen a relatively constant DC cunent of tZ lV "r.;;;;;r'i; a"ff"r.,aA0-"i above the plant t ps and providing a relatively ni cunent density of Zl0 picoamperes/piant, "orrrani and obseruea a 6i,Z rncrease in crop weight. A similar application of AC current produced an 8b% increase in cr ii ,H"51f;1iiii;11-:j}t:''d;;ilq;{:$ilhs*f i*;rui:im*:"r":iTi:lt";,"."x,'I::'|"I{:.:ir:1"Tili p;:*,*;*Fn, lhl'r:r,""'rl firiil viewedpast "[r,..,;,";;i:' *".r i, X"''5":;,lin',1llLt:,1',i: "rc"r.*"r't',1" j:"lil",,lif ;,i,ii,jiilff;!'ff;ilf::i :,'*'iii** t- :;;:ff :i::"* i!11i1{d:.,N:qi:',":$i inffS##": #it*ir#i;i;r;ili'l:i1.#'"{ii,1#*-lt,l*,,tI +,:.,Til'!;,lT,ix"Tfl s"x"fl.,xljl;',xllT;.? , Pl.nls responded rigouousty ro ar ,on srhutarron, whrch equared lo ::^fl::rr* ,l:,.::_p_*lT Fohaeeand. rru,r $m pres* pre bj;i"; 1"", "" i ; i:;;;;.";ij :ri;::"*:il:}fl i:i.jf*T;:.,::T# ili'i!::,Ll{:'il"ft;,f planrs, has potential to rnu.t"aty in".*."-"roo ditions,They found that a miid c rs capableof stimulatingo"* l*"nt of airions('1pA/cm2i brossoming^and i"*"*"Jg.",i,'r,l?ir$J:[fl.",:1",f infn*""". nilrririi u::ij:f:T:i:gjj.:i:ii*'"lx'j l":il'". the period of gro$.rh*q""J;.;;;;;;;;.;,; ,Si::", h.s more aeorbrc louncll ludenL r.rst for and cii.ic acid than rh r**,ffi ,,ru''"*"T*Ht*'liil:ihiiif :t"::":.rfi "j",';ru;ruju :Jil?:".i:*:: #';lih{i'fi:":"f; INTRODUCTION en, ed ) 32. ^p-r,, i|'"; THE OBSERVATION THAT atmospheric electriciry occurs not only during stormy weather, but in fine *"utt *ell (Lemmonier, I752) very quickly led nut,rra pf,lo.,ro'i"r. "i-o" to speculate that this constantly preva rng ,"".;. ;i-;;;; might influence plant growth. Father Glambatlsta g""a".r"."f lhe University of Turin (1??5) statea uat, ..tt appears mlniiest 'ei;;r;;";';. that nature makes extensrve use of atmosphenc promoting vegetation". This putative refurio".f,ip *.", i"J"J"rra_ entty^conceived and exptored by Berthoton ifZ83i, Cfiir, ( 1 7 8 2 ) ,a n d I n g e n h o u s z( 1 ? 8 8 ) . The discovery of air ions by Elster and Ceitel (1899) -Ihomson and bt { 1898 | made expenments on the biological effects etectricity more comprehensibte and uttimarety ::".,:.:1pl:": reoto tne development of suitable methods for their production and quartitation. In 190,1 Lemstrijm reported that an electricat dLs{harge from mel,3llic polnls placed abo!e seedline,p.odr""d e measurableslimular,ron of growth. ancl thrs oUseivarronwa" three years tater by cassner ttSOZ) g1u"[ilul .n] ::1":*^ a lonssenes orexp"".;",;';;.;;l; . o. rrn fff p'4,6.{19211^":13:"1-"0 In rne taboratorv on planLs pot rn culture, ard orr fl"ld"top" exposed to ron-prooucmg high-voltage, low-amperage -i; erectncat drscharges. They obrajned sgnific"arri rn";";; ,,1 pho- sor^1h,.anddry weighr at harvesr.Sidaway t19751 has revrewed .... ruu ntstory of what came t,o be ca_lted..electroculture... -"i"-"iro. {37-{.r: rrrso\/r'lwork has been concerned wrrh rf,",nnu"n"" of E ! , r it,4t.4f r r e r o so n s e ( d g e r m l n a t i o n { 1 9 6 ? r a n d l h e I n f l u e n c eo f 'reclostatic fields on plant respiration (196g). 1.253-25 sza, zsr 11,t96 at mol ol Biological phyiics Volume 11, 19g3 application. Nlurr (196,1, 196ba, 196Sb, 1966a, 1966b, tg66c) has s.t u d, i c d ^l n t e n \ r v e l yl h c b t o p h y \ r c 5 o f p l a n t g r o l ^ . t h, " . f , . , , r " statlc ficlds under condlttons pr( ":,';':i n#liy.*i*:l iq!:$.:;'ll:"".t#' fi wrthapphedelectrrcfretdsberow60 k\. m ancl fl;"1.1,.:,r"",, rvw Kv/m. respectlvely. Above these levels growlh rarts\rvar(. _;; decreased. \!lhen orchard grass seedlings ;"r" relatively high electric fietd strengh, ".;;;;J the planrs a"piry"J ,ii, damage and brochehical analyses indicared t'r,"t' ,ii-,i"Lri.j enzyme content of the tissuewas alcereo_ In the course of studies of small air .. ion action on net **:t :j::l prants 9l exposed barley,Elkieytt at (\e71Jnot"a *,ut r,J,.i to positive lons exhibited signlficanr rnc.eas",. rn height. and. dly weighr. Ea-rlier, lUaw fr"J ffgbZl growth stimulation oi garden cress treated with positive "lr"*",i oi negativeions. Bachman et at (l97ll experimented with -^ electirc field 30 varreries of ptants.\{rth ri"ta,r,e.,g,i, oi viltj::"il,:.." v \ r vw 'r v /m, a slzzlrng noisp developFd and the odor ot ozur,. t,ra\beanprovedro be ,*n,1,,',, .1i,9i-ll"Td; .The "*ouilr"tu ;;J;''ili['",ff ::i'"":'.:l',::'il'".T"', iil1#"l.iy""S,1, seq,uently, extensive experiments wele conduct€d with bariev , moniroring of arrron producrron. lli]'11 ano rne ilih presenceof03. Elecrnc fipld strenFhs "".o";;;;,.. of 200 kV m growlh. ln a range of eiecrnc fields thar rnctudp; :j.,]:lTd r,'use oc(untng tn nature, rhpy found thar sufficrenl corona ;il;,;'"J:"d'T,:'.,ii,",*':;:"iliJ"ff ;rTS,T:"1i: and concluded that growth is retarded by €lectric fields ) 200 kV/m, while below this level it is enhanced.Gro.wth stimulation is greater at 50 kv/m than at 150 kv/m. Further. the air surrounding the stimulat€d plants when vented inlo another chamber enhanced the growth of plantl contained therein. They deduced that the growth-enhancing factor is a byproduct of corona and that it develops at relatively low field strength. Growth retardation occuring at higher field sftengih is associaled with cuFent flow ftom apex to base of the plant-a phenomenon .reported earlie! by Cholodny and Sankewitsch (193?) and by Lund er ol. (1917). The mechalrismsof electrostatic field actions suggested by Bachman and Reichmanis support the hypotheses espoused by the 18th century philosophers that atmospheric electdclty, even in fine weather, acts to promote the growth ol plants. Zhurbitskii (1958) and Zhurbitskii and Shidlovskaya (196?) studied the influence of electrica.lconditions on the uptake of ions in solution by plants and iound that potential gladients equivalent to those prevailing in nature can affect the absorption and incorporation of hea!ry metal ions. Exposule to artificia.lly increased densities of small air ions enhanced these leactions. Similar results have been reported'by Murr (1963, 1964, 1966), by Kotaka ef al (1965a) and by Krueger et dl. (1964)- It is significant that an environment in which plants are protected from atmosphe c eiectricity inhibits some of their essential physiological processes and interferes with glowth and development (Zhurbitskii 1969; Kruege! ef dl. 1 9 6 5) . Our own experience in this field began in 1960 at the University of Califomia, wh€re we developed facilities permitting exposure of plants to sma.ll air ions Ln a controlled rnicroenvironment (Krueger er dl.. 1962). For the most pa.rt, our subjects were seediingsof oals (Aueno sdtrrd) and barley (Hordeum uulgoris) grovtr, in chemically defined media. we iound that seedlingstreated with unipolar ronized atmospheres of either charge produced stalislically significanl stimulation of growth as measured by mean slem length. integal elongation, and dry weight. The extent of growth increasewas roughly proportional to the atmospheric ion density and this in tum determined the magnitude of current flow to ground. The minirnal current measured in a ground circuit and capable of producing a measurabledifference in $owth was,l.3-4.6 x 10-13 ,{/plant (Krueger ef. al., 19621. Reductron in the air ion content of the air resulted in retardation of growth and loss of turgor (Krueger et al., 1965). The majoi biochemical changesaccompanying the action of alr ions on plants were found to be: (1) increase in rate of growth and dry weight: {2) incieasein production of cytochrome C and other Fe-containjngenzymes;(3) increase in Fe uptakei (4) shift in the distribution of Fe be- ----7 z5 r'i'4-;5'; aij ruos CrossSecfionVrewot Growrng Beds Longiludinal rnd cros-section views of the greenhous€.The ai.-ion Fig. l emitter in.tallalion and th€ location of lhe air-ion.lreated and confol planl! are sho*n. tween chloroplastsand the rest of the cell: (S) shift in the rar€ of darklight shrinking and swelling of isolaletl chloroolasts: { 6 , s t r m u l a h o n o f A T P t n e r a b o l r s mo f r s o l a t e dc h l o r o o l a : r s : (?) increase in oxygen consumption: (8) inciease in RNAase activity of leaves (Krueger et al., 1963; Kotaka el ol.. l96b: Krueger et dl. 196.1; Kotaka et o/., 1968, Kotaka et ot. 196i. Kotaka and Krueger. 19?2). With this background,we undertook to determine whether the growth stimulatlon observed under laboratory conditions could be duplicated with a market crop gtown in a hydloDonics (soilless culture) greenhouse.This experiment was conducted during the period December 19?4July 19?5. MATERIALS TND i\,IETHODS SeedlingHouse(SH) This structure, 9.6m by 3..1 m and 2.2m high, consisted of ribs and purlins covered with corrugated plastic panels. Exhaust fans at one end plovided air iirculation, and an automatic heating and cooling unit kept the maximum daytime temperature at ca 27"C and the minimum nighttime temp. erature above ca 21'C- The air ionization system utilized a hrgh-voltagepower supply connecied to four emitters (needles) spaced 61 cm apa-rt in a square pattern and suspended 56 cm above the trays which were to be exposed to air ions. It was operational 2,1 hr. a day. These tmys and the emitters above them were located 2 m downstream from the trays holding control seedsand seedlings. Experimental Plants Seedsof the indeferminate variety of Lomalo( Lycopersicon escuLentumP }liller). cv tropLc VFST, were seeded in moistened pellets and set in shallow plastic trays to germinate. Sixty percenl of the pellets were placed beneath lhe air ion emitters in lhe seedlinghouse and {97, in the control secllon. Ali irrigation, feedings, and environmental control procedures were performed according'to the growe!'s normal operational standalds. Treated and control seeds germinated 5 to 6 days after seeding. All the seedlingswere left in the seedlinghouse for 16 days before iransplanting into the greenhouse,where they were divided randomly into two groups: 864 plants in beds 3, '1 and 5, and 576 in beds I and 2. Ion flux density was greatest in the area of beds 3,.1 and 5 (treated plants) and least in that of beds 1 and 2 (control piantsj. This point is considered in "Discussion . Structulal Design of the Greenhouse The GH in which the stimulated and control tomato seedlings were transpianted is shown in Fig. 1. Essentially,the CH configuration shown is commonly described as a quonset (kamaboko) house. The p mary structure consistsof a seriesof ribs (bulkheads made from assembledplastic pipes with metal pipes as intercostaisIpurlinsl) and roof truss merhbers. The entire structure is covered with iiberglass-reinforced plastic panels. Cutouts are provided at the gable ends fo! exhaust cooling fans, doors, and cooling pad panels. Secondary structures of steel pipes are installed internally to support the natural gas heater/fan unit and the overhead air distribution duct and also for the necessary wile cables to support the tomato vine/ fruit loads. The GH is ,10.23m long and 7.92 m wide, and provides a total productive a.rea of 125 mZ. A covered belowground level reservoir to contain the nutrient solution and pump/valve assembly is located just inside the entrance door , Journol of Biological Physics Volume 11, 1983 of the GH. lts capacity is 4160 litels. The remaining floor area consists of concrete perimeter walkways, worklng alsleways, and five double-row planting beds, which accommodate a total of 14,10 tomato plants. The beds are sunk beiow the aisleways and are proLected from Lhe eanh wrth plastlr hners or barners. Ined gravel is used to fill the beds and functions as plant root support medium. The beds are periodically flooded and drained during the day with nutnent solution to provide tbe plant food needs and plant root aoratlon, The minimal night.trme remperature dunng the experiment was ls.C and the maximal daytime tempcrature was 25"C. Air lon Generators Negative air ions in this experiment were produced by a Klykon model 130/E109 generator lvith emitt€N positioned as indicated in Figure 1. The line of 28 emitters was located directly above aisle 5 between beds ,1 and 5. Since tle piants in tle aft section were 30 cm closer to the emitters than those in t}le front section, they received a somewhat higher dosage of ions. It should be noted that t}!e placement of emitters relative to the growing beds did not provide an ideal test of air ion effects. As is evident in Figule 1, the control beds 1 and 2 were not completely protected against ion drift from the emitters locat€d above berls 4 and 5. The longitudinal flow of air minimized latei-aldispersion, but did not entirely prevent lt. At eithe! end of the greenhouse the longitudinal flou averaged 1?5 ft/min. In the central area the flow frorn inlet to exhaust was ca. 90 ft/min. Air ion flux density at various levelsof the greenhousewas measured with a target probe and a Keithley electlometer. model 6108. The air ion flux density 20 cm above the growing beds langed from 8-20 x 103 negativeions cm-2 in the aft ionl treated area and 6.9 x 103 negativeions cm-2 in the forward iontreated area- Corresponding values in both the aft and forward control zones were 5 - ? x 102 negativeion cm'2. The ion genelating system went into operation 24 hrs a day three days after the seedlings were transplanted. Thirty days after transplant, operation was limited to dayiight hours. As noted later, there was a bnef period of deactivation 100 days aJter tlansplanting. Since as little as .10 ppHtrl of 03 is harmful to tomato plants (ReLnert et al., 1972) and corona dischatge type ion generators ale liable to produce 03, we wanted to be sure that ihe injury threshold was not exceeded. The cenified, exceedingly sma.ll output of 03 by the genemtor-emitter system employed and the enormous dilution factor imposed by the air €xbaust system combined to exclude 0l as an element in lhe present expenment_ Plant Culture and Maintenalce The grower's standard operational and environmental conttol procedures wete followed except for nutient adjustttlent and changes in leaf pruning and pollination necessitated by air-ion-induced effects to be described under ,'Results.', For this wint€r/spring crop during the plant matunng phase and through the imrnature green phase of the iirst fruit cluster, the nutrient folmulation shown in Table I was followed. Trblc 1. ulion r't The elemental cont€nt of rhe basic nulrient formula. This sol monitor€d every two days with a conductivity merer ard a pH El.n.n! ppdr 124 99 266 M c s 64 tI 87 Fe t.32 0.64 0.3 038 008 _. Dunng the course of fruit maturation from tmmature gre(,n through mature green and color blush. nutrient concentration imbalance became a frequent occurrence. Accordingly. excel)t for trace elements. a two-step lncrease(20t; per step) in ma]or elemenls was effected at each nutiient changepenod. i.e. every three weeks. Visual observalion of possihle advcrse effccts stemmlng from nutrient inlbalance was made three trmes per week- No other serious deficiency or toxlc signs wer{i noted except for phosphorusdeficiency seen in plants located nea-lthe r:ooling pads. This, coupled with chill, caused somo 50 plants to be discolored and stunted at the growing portion. Appropriate changes in temperature and nutient formula brought about normai ncw growth withjn a few weeks. Fnrit.and petiolc samples for analysis were obtained shorlly after the eirly nutrient changes. location and Weather The bydroponics installarion used in this experiment is located a few miles northeast of Grlroy, Califomia_ The weather pattern for the period involved is summanzedin'lable 2. Table 2. Summary of weath.r dala tor penod oi experiment- T e m p a r a r u r e{ C ) Monrh High Low Max Min Rainfall No. of Torat Day' tcmJ Overcast Da\'s 8C% Cover dunng dayll!r'rhr. R.maras Jan 16.3 1.0 2l.r_ -5.0 Feb 16I 4.7 23.9 |.7 Mar 17.2 5.4 25.0 O0 Ap. 18.9 46 1.1 May 26.5 17 8 3?.8 3.3 Jun 28.0 195 36 I Note: 1) Barometflc presur€ ranged fiom a h,gh of ??:il.:lmm HE lo, low of ?50.1 mm 2) Dala sourcer Na!,onal Cl,matic Cente., U S Dep1. oi Commerce 3) No unusualstorms 4) Cusly w,nds: 25 knors during latrer pa.r of monlh. occuirin{ berween 1000 and 1,100 hours P.S T. 26 I 6.r' 2 0.86 10 13.31 9 9 15 1510 7i 5 {.50 10 0 0.0 2 0 o0 1 Nore 3 No1. ll R E S UL T S After 18 days in the seedling house the air,ion stimulated tomato seedlings were 5O-757otalier than the controls and had 1.2 more sets of true leaves. During the first 30 days after transplantinginto lhe green, house, the seedlingsin the treated area were stlDulated 2.1 hr per day. No height or growth differences were noted between the stimulated and control plants. except that rnicrobuds formed on the 20th day in the stimulated plants and on the 28th day in the controls. Blossomsappearedon the 29th dal,in the ion-treated plants. On the 30th day in the greenhouse wc decided to stimulate only dunng the daylight hours l0?0() 1900) to allow io! a rest period dunng the night. Several days lat€r a marked elongation of the stems was noted in both treated and control plants. However, the srrmuiated planls exhibited a stem growth rate substantially greater tharr that of the controls by tle end of the 41st day (Fig. 2). This was equivalent to "plant eatiiness" growth of 20 days over thar of contlols. Fody-one days after transplant the gro{th rates for the stimulated and control plants levelled off and remained constant with tespect to each other. However, the residual "earliness" difference in growth rate temained about the same until 120 days aftet transplanting. Since t}Ie tomato plants were grown as single-vine plants, lntegr:a.l elongation measuremenl Yrnrguchi and Kru eger Electroculture of Tomdto plants in a Commercial Hyd.roponics Creenhouse cv Troprc,V_FST _ S e e dO o l e :! 8 J o n 1 9 7 5 T r o n s p l o n l e d4, F e b 1 9 7 5 220 mutot 2AO hrs/ tioylrghI S lr m u l uLt o n C o n l d- > StrmL.rlolron Doylrghh t rs only F[sl HorveslWeek-1 tompteleo : 7 cluslerssel ond sizrng E ; t6o = -E 120 E .-: a /,Slimuloted T o4l \ , Conlrol I iopped rrPs) ,,1(growrnq o') I "rc Fruii Formolron 680 Blossoms '-,' o o 2 0 I Tronsplont to 6 Horvesl Weeks t2 Fig. 3. Tomato planl yi.ld for airion keated and conkol planls plotted as yreld rale against harvesl weeks. I d o6l- No Fruil Formolton 40 60 too 80 Doysofler tronsploni ing 120 3.5 140 Doy Fig. 2. Plant g.owlh rate of cont.ol and an.ion.treated tomato planls. Th€ ordinate indicates a!era8e msin stem length in cenlimeters. The ab' scisa lisrs rh€ days .fter t.ansplantinS. Iltajor events characle.izing !he air-ion-trealed planls !nd controls a.e entered. was not possible. Consequently, in Fig. 2 only the main stem average rat€ of growth is shown as a function of days lrom transplanting. Notes have been entered in Fig. 2 to key certain growt}l events. Visual inspection was on a continuing basis tor plant res' ponses and physical appearance. The stimulated pLants in general had thinner stems, smalier leaves, less dense overall foliage cover and had two or three more flower fruit clusters than the controls for equal stem height. The labor requled for plant leaf pruning was much lessfor the ion_treatedplants than for the controls becauseoi the denser foliage in the latter. On the other hand. less effort was expended on pollination of the contlols because the ion_stimulated plants had more flower ciusters per plant. These differences balanced one anolhe!. Harvesting, sizing, grading, and packaging of mature fruit took place three times per week. Ninety.seven days alter transplanting the growth rate decreased for the stimulated plants and was only slightly 'fhis phase coincided approxlmately reduced for the controls. p,eriod when air-ion treatment was lnteEupted! with the brief as noted below, and produced no ill effect on flowers, fruit growth, or on matumtion of tomatoes. Virus desease was detected in control and stimulated plants during the sixth week ol harvest and caused a leduction of fruit yield. Diseased plants wele removed at a rate of 37o pe! week of the total plant population, producing a total loss of 257o tn the treated €Foup and 10% in the controls. The collecting of data for plant performance in terms of fruit yield and quality was terminated 152 days after hansplanting because of the excessive plant det€rioration and Iossesto virus infection. Harvest of fruit from air-ion treated plants began 104 days after seeding (86 days after transplanting) and proceeded more rapidly than did harvest among the controls. Figure 3 depicts the yield rate pe! plant as a function of harvest weeks for stirDulated and control plants. Figure 4 displays the cumulative fruit yield per unit of greenhouse area. Because of the higher incidence of virus inlection in the ion-treated group the yield curves begin to converge at the ninth week. Figure 5 is a plot C ocll 30 25 )< ? 2.4 E d l on t s S tr m u l o t eP Avercgesof Rad< a 4 6 1 5 l ; o ) \ / / C o n t r o lPl o n t s A v e r o g e so f B e d s AJ lo E f o.5 o -o Fig r 4 6 H o r v e s tW e e k s .J F r u i t y i e l d a s a f u n c ! r o n o f g r e c n h o u s eu n i t a r e a St i m ul ot e d Horvesl Weeks F i s . 5 . C u m u l a t i v e f r u i t y i e l d p e r p l . n t f o r c o n l r o l s a n d i o n - t r o a t e dp l a n i s p l o l l e d a E a i n s rh a r u e s rw € € k s . Journal of Biological Physics Volume 11, 1983 eili:r: cornpos,r,on rh. riu,,comp,,s,1,on da,a.eprcsenr. averascs or anarvses con(ruc,ed by ,heun,v.rs,,]5 111 r n c i n e p e r r o l ec o m p o s , r r o nr r * u r $ r c r { ' r r o d r y w e i s h r b a s r sd r e p o . r e d b y b A R D C ( o h i o s i a r e u . , " " ^ , t v j . Il8i3i"'iHll[';:%11.:Ijfi:[: w o o s r e r ,o h r o . A n a l y * s . F . u r r C o m f b s i rj o n (Ave.ages) Ripe Fruit pH from EC Bnx (ppm) (') ac'd Ftavor P Good conrrcl (%) (ms/t0os) 1.4? 2300 5 OU 0.31 S r i m u l r t e d4 . 4 O r ? 2 5 5.rS 0.36 Conirol acrd Analyses . Pet'ole Compoel'on (drr wt bas's) I t.t Bett€r € r i o t e N p K c E" a M g Na Si Mn Fe I ? 5 0 . 9 3 6 . 3 6 t . 3 0 0 . 3E 0 . 1 1 0 . l o 6 9 99 S t i m u l a t e dr . 8 0 l . t I 6 . 0 2 r . 58 0 . 5 5 0 . t l 0 . 1 0 8 6 l l 0 of the average cumulali!p yreld,plant for controls and treated planIs agalns! harvest weeks. lndependent analyses of fruit and plant (petiole) wete conducted on samples talcen during the fourth hu_"at ,u""t and t}le results are displayed in TaLle 3. fr,""" J"t"-.ai""i" the arr-ion tteatmedt imploved Cu B Zn Mo 6 2 6 2 6 lt 27 36 Sr Ba Al I lt 61 2l 59 2 . 97 6 5 2 6 5 3 trecome a dynamic and viable industry in the United States. Although the United States lagged behind Europe in terms of gteenhouse acteage, the steady gror,tth of the industrv is begrnnlng to close !hrs gap. prrmarily bpcause of lncreasing demands for high-quality fresh vegetables and flowers at reasonable cost. In general, the greatest concentration of greenhouses in the U.S. is located in the Eastem zone, notably in the state of ulated and control plants are summarized in Table 4 Ohio. However, other states in the South and mid-Southwest are expanding theu facilities. Dunng the last few years the industry in the Pacific states has begun.to show rernarkable DISCUSSION growth, especially in the production of greenhousetomatoes and European-typecucumbers. During the past three decades through the impact of t€ch_ Although tbe industry has been on an up-trend, the impact of recent crises in avaiiability of energy, escalating piices for _nological and horticultural bleeding advances, the greerrt ous. ploduction of vcgetables, flowers, and bedding ptan* fuel, material and supplies, and for labor have imposed a sereve tras constmint upon plant expansion and facilities. These factors bave moved commercial gtowers in the United States to look olsed.rrcns compadnsrne r.sur(sor nesarrve ion JlllE rr€ar. --' for the implementation of technological and horticultural m.nt or -1 lhe sp nt rornaropjrnr .rop with a c".,,"t r r.i*r..a1air*p advances that would provide them with increasedproduction and profits within the existing facilities. One potential element I PLANT CHARACTERISTICS TIEMARKS in the area of technological advances may well be the a S l e m h € i g h Lg r o w t h E a r l i e rb y t * o * e € k s application of air-jon lreatmenLb . S t € m d i a m e r e r ,d e a s e s ( h a r u r € s_"rr_,i.e pranrsj Desplte the fact that as long as 200 years ago atmospheric "-,i).r"-ro. controt ( a r e a ) ,d e r a s € s ( m a r u . e S m a l t € rb y 6 0 % c. electricity was suspected of influencing plant gro\rth, the ro 7s7. !€af.size requirementJ for critical investigation could not be met until d . C l u s t e ri n t e r n o d ea v e r a g e s C t o s € r il a c r n v 6 2 5 c m f o r 1899 when air ions were discovered.Since then, wide-ranging e Number or flowers pe. crusrer. *j""[tj?/"-, o,rr***" int€rest has developed in a whole spectrum oi air-ion effects on livlng forms. The literature contains many accounts of f . P e t ' o l ea n a l y s i s See Table 3 experiments in which ai!-ion enriched environments have been 2 B U D , B L O S S O M& F R U I T used to treat diseases,e.g. asthma and weather-induced svnFORMATION dromes such as the sharav tllnessof Isreaj Ths phaspof au.-ion e . B u d sa n d b l o s o m i n g Earlie! by abour two weks research has not been implemented in conventional medical Earlier by abour rwo weeks practice, largely because of failure to meet the requtrements c. Fruit ripening Earlier by abour l0 davs d. Fruit confo.mation and qualiry of satisfactory experimental design and neglect of thc Dlacebo Betler by 10% b rS% ior Crade I effecr. Str-,rdiesof general brorogrcal effe"rs uslne bac!€ria. More of la.se. sjzes(.{x5s & protozoa, higher plants. insecls. and higher anrmajshave bepn r. Fruit compositronand flavor a.u""til," , more productive, to the point where it.now is possible to state that arr ions are biologtcally acLive. As noted ln t_he int!oJ. FRUTTYIELD RATE PER PLANT duction of this pap€r, there even exists a fair amount of inforr. Yield rat€ per we€k Createi by 50% in fir6t 3 we€k6 matron regaJding the mechanism of air-ion stimulation ol plant b. Cuhulativeyield rate G . . a t e . b y 2 ? 7 oa r e n d o f 6 w e k s c. Sp.ios.rop yield growth. E q u a i l e dt h e p r e v i o u sg o o d s ' r m l n e rc r o p ( u n l r e a r e d ) Our goal in the present study was to determine whether the air-ion enhancement of piant gowth, so teadilv demoni. NUTRTENTS strable in the laborarory. occuls on a large scale unj", ..p.u,._ tical" conditions, The €xperiments were conducted with tomato plants in a Ca.lifomia hydroponic greenhouse facilitv where 5, UNCERTAINTIES tomaLoes and cucumbers are the primary vegetable crops. . ai'.rondosaeetevetrcqurremenr ror othercujrrvars 9ft,."' (soilless culture) was chosen as a first-choice or Hydroponics ar srages of ptanrmalunty , dirfer€nr t€st bed program because of it flexibitity in the control of such variables as nutrient formulation and concentlation. extent t'ruler and funsi andon ftyingor.rawtrnS in;ccLs. of irrigation, and environmental fac6rs-r poss'b,v duero b'o Out experimental design included observations for air-ion fli;f#.r,::ti:I.,;:1"il:li:"jj",*T's induction of (a) growth stimulation, (b) accelelation of fruit -o fruit.. Generarob."*u,ion. f i il|"",""1'"1''il",i,j*t1 ",.T.:lH: .' ilff,"""';:*Tj,l$fiil[:..,1,1 * j;ff ,]3.1;,:'ff #,*:::d"* "";i,l#Ti:;:"t""J:":;."i.,'::1"",::l?i':".";Ii:315:'rH:,. Y8eaguchi and Krueger Erectroculture of romdto prd ts in (t commercior Hydroponics Grcenhouse i maturation/ripening, (c) increasedcrop yield and (d) improiement of fmit composition, We recognized that in such a pre' liminary exp€riment it would not b€ feasible to conduct a definitive test employing the control conditions one would impose in the laboratory. Ideally, an experiment of this sort should utrllze two spcllons of the same greenhous€.one hous_ ing the treated plants, and the other the controls, completely isolated from the artificially ion-enhanced atmosphere. For plactical reasons. we were not able to install a Faladay cage to pervpnt rons from reachlng conlrols. .{s a compromtse.we madP us. oi th" fact that air'ion density falls off rapidly with distance from the ion source- The emttters were so positioned along the length of the greenhousethat beds 3,'4 and 5 were closest to them and could be considered io present an lon_ireate<l area (Fig. I). Beds I and 2 were far enough away l"o sPrveas controls although they undoubtedly recerved a low dosage of ions. Consequently, in the absenceof a completely untreated set oi controls, any diflerences in biological effects observed during this experiment could be ascribed to differences in air'ron dosage. C)n the basis of the aveEges oi the ion flux densities (number of ionsTcm2/sec)at plant level in the three different areas of the experiment. ion-ueated plants in the ait section received ca l7 times the dosage of planls in the control areas' while ion-treated plants in the forward section were exposed to ca 13 times as many ion as the conirol plants. It can be argued that the plant responses obseYvedon our experiments depend on differences in the imposed electrical fields. Clearly, the control plants lvere exposed to lower electro_ static fields than the two groups on ion-treated plants. Howevel, the work of Bachman and Reichmanis (1971) and our own expedments (Krueger ef cl., 1.9?8) demonstrate that ai! lons are the primary element in conveying the small electrical currents to plants that result in increased €te of growth. In our exp€riments, conducted in very low electrostatic fields, no growth enhancement occurrd until air ions were added to the ambient arr. Bachman and Reichmanis found that increases in plant growth, in the absenceof added air ions, depended upon the intensification of relatively low electlical fields at the pointed ends and fine hairs of plants to such a degree that corona developed and air ions rvere produced. The electrical currents requiled to stimulate grolvth are quite small: 6-10 pAi/plant in our early studies (I&ueger. et. dl.. 1962), l0 pAi plant in more recent ones. and 10 p-\ in Biackman and Legg's (192,1) series- Pohl and Todd (1981) reported a cufien! of ;l pA/cm2 at piant level to be effective in erpeditrng Lhe growth and btossoming of geranilrmsand Pemianviolets. The goal of the expedment iecorded here was to evaluate the application of electrocultule in the production of tomatoes in a hydroponics gleenhous€- The genelally favorable effects obsewed lead to the conclusion that the air ion ennched environment was responsiblefor: 1. Earlier appearance ol buds and fruit by two weeks. 2. Earlier fruit ripening by 10 days. 3. Cumulative fruit yield rate per plant geater by 2'l7o at the end of six weeks. 4. Supenor fruit confoldation and quality by 7OV-1,5Vo for Grade 1. 5.lmproved fruit size. 6. Improved fruit flavo! and compostion. Since the costs of insta.lling air ion generators are modest, their energy requirements are minimal, and no detrimental effects of negative air ions on the personnel have been observed, this procedure appears to be a useful addition to greenbouse technology. ACKNOWLEDCMENT wc pxpres6our sincprpthanks to Mr. Rlchald Finger and Thomas Michel of Klykon lnc. of Flonda for their interest and help in making it possibleto carry out this study. REFERENCES Bachman. C.H.r Hademonos, B.G.i Under*ood, L.rl'l. J AImosPhel Tetresi Phys JJ. 197 505. B a . h m a n . C H i R e i c h m a n r " . M, t * 1 1 9 7 3 .h l J . B i o m e t e o r 1 7 , 253 262. B e c c a . i a .C . B . L 7 ' 1 5 .D e l L E l e t t t r c n oT e n e s t t e A t m o s f e t i c oo C i e l o S e r e n o B e r t h o l o n d e S a i n t L a z ^ t e : l ' 1 8 3 . D . I e l e . t t t . t t e d e su . g i l d u r . P a r i s . B l a c k m a n ,V . H . i L e g g , A . " 1 . 1 9 2 1 .J . A g a c S . r l l , 2 6 8 . C h o l o d n y , N . G . I S a n k € w i r s h , E . C h . 1 9 3 ' 1. P l o n t P h y s i o l .r 2 , 3 8 5 ' . 1 0 8 E l k i e y , T . M . i P e l l e t i e r ,R . L . i B h a r t e n d u ; B a r l h a k u r . N . l 9 ? 7 . l a l B i o m e t e o t2. 1 , 1 6 . J Elsi€r, J.; Geirel, H. la99. Tettest. Mosdzin. 4. 213.34 Cardani, C. De infiuru electicitati' 1 78 4 . i D i s e r t a t i o n ) . otmosphencae in Degetantio,Tlurin, Gasner, G. 190? A€f. Bot Ces.25,26. IDgenhous, J 1?88 1'nfluence de l l e su e g i t o u x J p h ! s . 3 2 . e t e c t t i c t t io t m o s p h e t i q u es u l K o t a k a , S . ; K . u e s € r ,A P I N i s h i z a w aK . . r O b u c h i .T . ; K o s u r e . Y i 'Iakenobu. M . 1 9 6 5 .P r o c . A o t S o c , J p n . C 2 | , 4 3 K o r a k a . S . , K r u e g e r , A . P . t N i s h i z a w a ,K . i O b u c h i , T . ; T a k e n o b u , M . i K o $ r e . Y i A n d r i e s eP, . C .1 9 6 5 .N a t u . e2 0 8 . 1 1 1 2 ' 1 1 1 3 . Krueger, A.P.i Kotaka, S.i And.iese, P.C. 1962. J. Gen Phrsiol. 15. 879-895 K . u e g e r ,A . P . i K o t a k a . S . i A n d . i e s e ,P . C 1 9 6 3 N d a u r p2 o o . 7 0 7 . 7 0 6 Krueger, A.P.; Kotaka, S.i Andriese,P.C. 1964. Int. J. Btodteteor 8. 5.16. Kruege., A.P.; Kotaka, S.i Andriese,P.C 1965. 1nl. J Biometeot L 201-209 K r u e g e . , A P .i S t r o b b e . A . E ; Y o s l , M - G . i R e € d , E . J 1 9 ' 7 4 .I l t . J . B i o m e t e o r 2 2 , 2 O 22 1 2 . L e m s t r o m . S . 1 9 0 1 . E l e c t r r c L t rn A g n c u L t w . d n d H o . t i . u L t ! r e . L o n d o n . Lund. 8.J.. et al 19.1? Bioelectnc Fields dnd Crcbth. Unive.sity ol A u s ! r nP r e s sA, u s t i n ,T e r a s . 3 9 1p p . Maw. G.M r96?. Con J Plonl Sci ./7. .199.i0.1. Mu.r. L E. 1963 \ol!r? 900. 190 M u . r , L . E . 1 9 6 1N o l l r e 2 0 1 . 1 3 0 5 1 3 0 6 . M u r r ,L . E . 1 9 6 5 aN . a l u r e2 0 6 , 1 6 t 'a 7 O . M u c , L . E . 1 9 6 5 b .N o r u R 2 0 7 . \ 1 7 7 . 1 7 7 6 . M u r r , L . E . 1 9 6 6 a .l n 1 . . . / .B i o m e t e o t . 1 0 , 1 { 7 1 5 3 . M u r r , L E . 1 9 6 6 b . . 4 d uF . o n t ' e r sP l o n tS c i . 1 6 , 9 7 - r 2 O . M u r , L . E . 1 9 6 6 c .l n t J B i o m e t e o r .1 0 , 1 3 5 1 { 6 . P o h l .H . A . I 9 ? ; . I A , o / .P h y s r . I : J . Pohl, H.A.; Todd. G.W. 138r. /n/ J Biometeot.25, 309-321. Reinert. R.A.i Tinsly, D.T.i Carler, HB 97, 1{9-151 1972. J. An Soc. tlo't. Sci S i d a v a y , G . H . 1 9 6 7 . S p e c l f u m ,2 l i , 3 0 3 . S i d a w a y ,G . H . 1 9 7 3 .J . E l e c t r c s t o t i c s0 0 , 3 8 9 ' 3 9 3 S i d a w a y .G . H . i A s p r a y ,G . H . 1 9 6 8 . 1 n t . J . B i o m e t e o t , 1 2 , 3 2 1 - 3 2 9 . T h o m s n , J . J . 1 8 9 8 .P h i l M d g . 1 6 . 5 2 8 - 5 , 1 5 . W i n t o n . R . . e l d l . U n p u b l i s h e dd a t a . Zburbitskii, Z.l. 1969. P.c 100-l12. U S S R A c a d . S c i . . B i o l , S e r i e s# 1 , J a n . - F e b . , Z h u r b i l s k i i , Z . I i S h i d t o v s k a y a ,L L . 1 9 6 7 . E I e h t r c n . O b t d d . M o t e t 6 . ? 0 -1 3 . died on Deceibber 8, Editor's Note: Dr. Albert P. Itueger 1982. Please !efe! leprint requests or correspondance in cat'e of of Biomedical and En' Department the Air lon Laboratory, of Califomia, Berkeley, vironmental Health Sciences, UniveEity Califomia94720. 10 Jouraot of Biological Pfiyslca Volume I l. 1983 j t : FRUIT TREES e.".'1 I ) I When water ing also firl piie irh wacer. conical I coit J 8" ro to' , rUoR, H g, oE Groun d teveL p -, r 1 D et a i 1 , o f t r e e An tenna . Alwavs be careful E h e a n E e n n aw i r e s o t ou ch th e l eave s of the planEs or bu_rning could -_rE rac"I n o ! to allow t FENCES Chain Link Barbbed Wire Ground Leve 1 FENCESBECOYEANTENNA -2t- t-rl POTTEDPLANTSOR PLANTERBOXES leve1 Ground leveI flanter box may be any size convienent for area a v a i l a b 1 e , T r e l l i s e s can be attached for climbing They rnay also p l a n t s - P e a s, b e a n s , t o m a t o e s , e t c . be used indoors wi th Grow Lights, Coils should be cleaned twice yearly. To do this imerse in a oail of hot waEer in whi;h L box of soda has been di solved. 9 turns of wire to form a conical coil. -22- N o r t h o f t h e Equator the c o i l s s h o ul d be COUNTER CLOCKWISE. South of the Equator CLOCKWISE. JuLY 16, l 966 N AT U R E BIOLOGY s P Pd{ r p! o i J r h i b rpt l s r r , l o v . l o p m a . tb, u r f r o r rr r u . l , r , . 6 r , r , r tnflucnce or Erectrostati;_Fictd: on sced ;!ii.il",l"'X[[,lu"T;I*r"l[1,.1T,$H.*.:il,;ili:i: Germinetion and not, moroly by_lho pnrorrco of ch{r ,iold pr ,.. "llrr3 'E' ESr rrtr.strgstiors lrave stimrrlg!.d urdr.r irru-rut in possrblo illfluoncoof lighCin rcducing rho magDrr uJo of t hrs lriologic*l sigrriticarrco of nstura|y o"c"rrtng Pglf i{ offoct ms,-y-bs siShificanr tTiblo 2). "A comparison f"*,.b|,: ". ' . r i r . l i , l , l s o r r d r ' l r r t , r i p h r . n , , r n n r r nY. u r r r i h o s s h o w i r r i t h t h € w o r k o f K r u e S € r d d . l i s n o t p , , € e i b l e ' i nt h e 'urLlrrgh I|'t' ryrty,l,r'trrc ticlls uppoor g,o rrrlrr[:itdnvelop- lLrrcnco of- ion.donuicy ind currorrt mrdu|..tni.nts, t)rrt, Ed\rsrddr found thar a posirivo 6eld slouerl dorvri Lhtr rr.t irr grues r{'{rJlrrrg"; hu porrrts out. honevcr, th;r ol dcv"lopmenr-of i'erytir pupsr/ol.be slso suSSestLd rrir, r \ork,'rs, noebl] .lorgor,scr, and prrrxtl"J... and ilb thoc conloct-induced ol,.ctroiratic ihergoe rnight iriiiuence l:rl'rrs'rrirt )rnd Shrl,ata', obserr,rl rncreus.s in plant J orvoht tndcr elrrctricfield conditioDs,a lthou gh tho frcouent ltlsect b€baviou!r0 r ,l rrrr. rcp of ' onrrodrcror.y r"srrlte irr muc-h of th. ;arly Tho prosent Fxpcrlmpnts *cr.,. initiatorl In an a!&empt tl qryvid.€ a Possibla ba€is for corrolation rrr-rrra{'t,n ork this subjoct lrts boorr ornphasizcd by Lund:. goo'h, _oD pL)'sroiogicaleffoctsofair ionizstion lra\.Fbc{;r jtrtdrcd electrjc phcnomens sDd tho msniffststion of psychia'tric t ' trttmt,tr dtsturbance. Rymptoros, d^qsuming sucl, a rllariorrshrp Kru.gFr pl ,/.! rcp',n li,st "f sork"rq:. \lr,'lrI, lo I,oriuv"ly or negstiv.lJ' iorrrzo,larr produced to r,c nlFdiacc(l throllgh. !'sriatior€ in n, rrro-Lormorre rr,'r'rnJrid gcrrnination ii Atena aali'o sAdds qirh suh. activity resulting from induced chdnges 'l,r,rt ir,' ro,r.sFbirr grourh arrd dr). qeiglrtr slight rt] lr8sue r€sprrarion _elecJroststically l"r'cls. Preliminary gas.lr'al).,rs r. r' a\, s rr gro\-th sFr,. Rlso not,,d srtL r,...lhngs pla;.d appear Io rnrlx.si€ s possiblo polairtj-depcnd"nt ftudr,.s ' Porrlr\. or negati\'€.lo.tric fields of 95i V over i0 irrflucnc^ on tlro rat€ ofp;duction oi /"loctrGtaiic "m: "arbo,, :r !hl's{ €\l)erimonts, hou'evt-'r,tho seed r:ontainers qere seeds. Further work sloDg Serminat,ing thr'se ldioxr,Je.irt iroulded to p.event tho accumulstion of surfacc chsrqe. 'lrnoa is in progtess. I ' l n 'p r . s . rrt ' . o n r r n u| l i c s li o n d i r . c t s s t t € n r i o n ! o R n a p p ; r . I.!hankD.U.H(.stlrcoterbrcarr;irrgourr1,,.slatistical 'r t d^pendcrrcc on polarrty oftho response anallsis: I rlso rttan|r Prof. U. F. lrpre1.. Dr. J. R. ofcerminliing '''r,ls l,o llrd,rc^il clc.lro.r.Rric.harg.r6. !-rlr.rrrreton urr.l Ih. A. C. Lr',,rr for rleir ad|ic" ar,Ll I-otrr I's,1,he'j-r'f t0ri *+.ls of lJttuce llactuca satiL\1), encorragement. .'Clrtso€d Boro glr \t-orr(lor', v,ere sown on filtnr G. I{. SrDA\r..\y I ufroty r-lild or cdqh of fo'rr r) crn sq,rero s.{rions of alumi. -Depcnmerrr,of Bor,arr5.. I "'lt lr, . ,rrnr r . D r frorl,aDtaclrad o i l a t t a r l r n d bOJ'a ) - s r thrn h i n f ltilm m o f .of V aVa.seline'to . s e l i r . . t o t h the o u pupp€r p€" U n i r . c r s i t yCollege, Unlvolarty collog., -,rrtuceof a €hcot of gldss {J crn x ?.5 Cardifr. 3o;1 ll ,' ft srnrrlarly att,Ache,lro t,he rrndcr-surface "-. of a"u"6';ir1" second ,}Iur., t. X.,,va1u,.,200, 190(t,Jriu). I gloss r"sllng on iPuc'.rs so arrangcd as tO s^parot€ | Yur, L. E., Prtr. Pa,, Acad...a 3? tor (lgOjr. I l!'r oI ' } l u r r l - E - v a l r ' 2 o l .l 3 0 i ' t J , i r , . I t " ' ' t , " s c l s o f 6 . 1 , 1p l a t c e ' b \ ' " v e r t , i c a ld i s t a n c oo f -iilbl'iljji;H:lir;.i,:iji::iii,#.iiirjililr | 41;*1.ry;":"ri.litf."_j,":."""T,:rJt:,,R":l.X I / L 'f prir Pnsrtn,, o','. ncgaii"c nrrrl rha other l d ,l ,8,t'':}i {l r: .,o, '' 'r,o. o \ . r p o \ r c r v e r r,h. romnrnins pair- u"r" 1.i ,r r,AIg,rr ns.n,,rrolr' 0t', r.]dll:. posirions of.,hare.d ,r,'i 'ni L*'g'.,i pl,rr-. w"1, "hnr,8,,1 ufr,.r,.acl, r, t,'ic,rrron. on, lr I'rr, lr nf :roods w|rlr moisti'n..i \tith \lf"f \o\ll'H' ' oiriin,.r t., ^^o r . - o t u " r , .r r , r , , r , - . . i . ' 3 - ^ . ! . 1 n o ! . n : e o ! r : ) . 'f,run8{ A p.. ri6t!t., c. L i j . \ n d r r , - ^p. . C . . . / .t ; . r t , \ - - , . 1 . 1 6 - - . . 1 _ ,r0.",r. n.ri .\,,,,. L o r . , : , j ( r e , :, . ,.;;;,.,r" r, t..a^d,t | 2"r.rE..11 rrr,r. , Ed\rnrt r x.,r.d,orr.r z - , t t i - ,i , : , , t r , , i . : . , i . " I I 4'.']-;,'t.",.1'r,tlrtup1,rlratusbe.ingthent.ncl.'sr,tlin . . , . \r r , . r , , , o j r i s r t . . r d n d p i , r . . " d i n n n r , n s r . r n t l,.nrrr,.rdture r.,rrr l;rr i,{ lr. }[oqt of tlr, ,.xp,,rinr,.nrrrrr r,. rorrJrrnuerl ':li ""r(' rond.cted :.-,ii .l'.?:*:'il: ;i,:r.''in)ents pq-some S o m e p€': gil ; :,11,,ir,:il:rr*:fr:#fi*.*$it ; ic;ij ;:iiii: I :;i:ll =; 1: ; :I E I: : .- i ' ii tr:# +; s tii;;,r r-["*tfi;+:ur*ffi$$H I #i*s'*i,i1 ;i,ffgs*51,**ffi | .drrir\i ort ill light;rnd r,'iults luvrr. riark roorns hormvor'. Irr.r,n obtni T x h l . r, , , F r n R . \ c E , s p l * r r , \ r , , i E o . i x r ! ; , f f i ; j- =t = :, :]= i n , m . ,u- ' . i : T n ; ,II, . f B e , E = r - . . ,g i E ; " i $:t; , l f g s ; i ; '',,,,..1"1il',,,:,,*i*",.-, iSfqt!; ; fr6 ;;; ,n!.'{R{.\i'.""_T."ir;l".'.",rr,.--l_;il":j,,.._ l._":i1,",-:*, I iEt: 'J,F: EA;:: s.g;[:: DitrereDce iJ | ! l tfi55E;siEIgE: ;i; "'rrrTn"ff"'" a,,\.r,.,;,:, 5 i | EiF;:g€$.gf Igi:rtis-ss . ie=i11,i1"i_ i'F iF - : ' I ') jV?=7, =:Y?17? - , =-,i,i!i7?ri|='-9? a L ; 6 - 7 ' 7' ", ; 7 2 z=i. ,i \ i " ? i 7 E E ? ;',it 7 *Z=,tuitr,z7i77l,:Liziii=i?i: !, -_ t-7-Zi7 ? Z ; | " 1 ;;t-|'a?i=;? . 7 - 7 ; - \ z ; zzi ;; ? 7f d i =L : a?,r2, i=; 7/ ;l Vt | .=; i7:, =-u-: :..= t L1 ? z ;,7. i |li':i11Zii7i1'=;1, i :./:/. z'_.1_-=: =i 7 ;==Z : , -Zi7,2i =, t ,t ,i t - ' t 7 - ;i?1 7a ' =a7?ii-: , 7 ; i 7 z 7 Z Z 7: i i z -. . ', i z + , : z a: ;*z:zi r , =,'== i'-, a -, : - 1 "=7-: =,. ?-7 z 1 = = I L ? ?= 7. = a; ' 7 : - ' 1 : V z : ' t - V; 7 7 :- . .= ' = i - it :- i . = : i i ? : 7 .- . _. , 71 7 : - :- = : - ,= : l ,. , 2 1:7- = -;it,A_ Z ,7?7. '?- ,r izL' zi i i-7i .",7 7 u -; i , ? . ? , iy,1. ; c =.:'z 1 ? ;-,-= = A , I i = -= = : 1 = , ; ? 1 t - . ' r . - , r _r71 " i = = . ! = ,a - r ': = - . = . . : 1 i . ti i i 'zz' +7z' . i=; ? .1 : - t ; Z : "? i 7 '-= 7= _ . _ d,=,: _i;=.= . :- ,? r : 7 . a , i); ' , " 2 :r7= Z ' ; ? , , ? 7= Z z 7= 1 2 : . ,- 3 ; =i u,,P =qii: 2 :=. j : ? . = - r 7! . - ; . L ; i _ 7 1; =: _zZa1 Lt ,: . =- "=,1 ? Z'=t=:;7 ' ; ; ? + li -, i. t==r '-: 4 ; = . / . 1 . 11 7 c l':=l:.. : t = 7 = ^ . . = . . ^ = =':i=i= 'i,'^2 3 1= i' :- l; = i t = i -7Li=) ,1.v I = , ? - 3i t- 1 i = 7 . - l : _ ==- = ; . = - , = 27',i.- 1 1 . 1 7 7 V7 L 7.: ..i.7:: = ' a. - " - ;=i? a a _ 2? -; z ' :, + . i i zL_}. t = = i - ' = i . = l- , - -l- ?' '., ', '-=,7';: '.i l,,V7rrrr= --._117-'=,?, t .'z . : ' ; ' - /' . - =;.:=== - = 7' .' = = | ; - = : i : - : : -= a-'- - ''| r- ,, ,- r.. - -l( - _ r t . - n l ? ; 7 z --_i z 1 = e ='-.-.?,8= -, 't7{ 1 i - : i7 = 7 ? _:'. , i17ii 7 i = _ = . 2i ._. ,7; =7=-.' 7; =; u ?L:'1 i _ 7 : = j i 7 : .7 t i a. 1 . , = =1) = ' r i : i ' r i ; 7 - =f - i . . i l r 1, l -?,1== i ".ri - 2? :_: ? - t ?i 7 ' ' - = '.,, . 2 .: j = ?j , ? 1 : l : i z V = : - =- 7 , z i 7:,1_ f 3 7 s Z = ' ; . 7 . i' ' : Z . ' - c - .2 = - ' ' : , 7 i - 2 ' t ! ' E ' L 7 7 ?r -t 1- i c_a i = 7 . ; j i " = ' 7 ^ v ,.-:' . c a a u .\ Y ' ii. i : d ? ; e 7 1 i t = i - - i . _ . . : : - Li -- i .-; I tf, ' ' t l E l i\i - c F i i i ; EE : El E t? i - l 3 : L ? 1 q 7 i , 71: :_; ? ai 7 : ?I : : i - i l : .= ; i ? - i l 1 I i - li a li t t , 7 \ -= j ' i - 1 = l r . ' ' - . = ' 3 . = i 'i . : a l Z i . ,= 7 -''s--r';, -n 3 r-- a. ; 2 : i = 2" - i i z ? , 77 . = - , 2a - '9. i2z'zz:za= ,- '': ? . 4 i t 7 - = ,=: t E : ; i - . - : =i , v " i . = . ? ? :. ^ : 2 ?.zi 4 7? ?_;Z z 'r7 : 2 ?-i z = - ; ? t ? i'=z aZ iL 2 = z r : ?t i ; . . 2 i :' ; ', r i - - = a z I i 1 - 2 ;i ;: - ' 2'= a .:- 4 7 i e"7.? =-i 2r- l z i- - ; i' i ; 77 ? ^ ? = n" + . + : ' ' 1 - |I d^t t 'i4 ,. cl,.rt' x. vq'&t, I' c.t rrrlll, 9m o9?2), t!- S, M. P'*Eh.l, Entho^ H.dth P.rtPc.t l, L H.t* }|. S.ptrlrlor.nd It (l9a): t n, a, QYn. D,. t. t,A Y.d t''rE. B Clrtron' P. J. Grr' la. t O. L.aldotn, H. T. Dclvt+ W. C. ,. St Ps-4 L.!t, rft+m. J3t alYtlri ?, t f!, t. I-.n.. R W. Bdoh R. H. wlittonh' bndrit.t, W. l{tb.l, A. F. no!.& F. N. W. $xhUta, ol d al rb Coal4oc! blu , Fpq F!*i|t PoLonlta co'orrol P.oi.ct' lrrd Ctll&ood MrY Ailuu' Cclt . to. Dtt t Cdrol, lna. l.*rtct Nrltoad tt. s.r9Dorrcd bv C|!.r|llr Aa6a/i. W. th.!t $. CouDc{t rc..rch t'l'! Pubuc Hc.nl' lorrd ot ra.t ot tb Totolto lor $ct 6. l{o?hd ft dah Dcaddq . t l|bftY of & ChU(t.|L |nd fu htlo Cdn* aoo"..alr6. ld ibl Eavlroodat ort|r!.d bv t.'ri nlty Fr!.nl!t DtolErm. aroCrtlocri Dr^ l. A iltt locd ..tatat-a .!d S. M. M.ct od colL.r.d blood ard hdt t<. .!tts6 out tlt ..mrL.. N.lrmlodc.l by I)'. O. Mur!f,y. Tt Al"A |!.l coFoFr_ cttrLd out bY Da. I. ntt r l'|rrhyrlr Y. OEYrr A. V.ul& w. tl&t lt6D!.t lt' to. L.lalcrl tad F. fiulr WlllhE, l.|w Envloat|Ent.l .n ., rn<l ttr c|n.dLr Atloclatlo! la rdvlc.. oa t ra!y. UntPraraa rddraar: D6p.tidar Eodrd. E.riry of Lirc'pool Uv.rFol n...s.s Tr... Ad.lydt Pr.aa d&.||: Hrlif|r, NoYr Ccntc., D.lhousic UnllGrdty. Scoalr. t Autila l9?a Virbility of Storcd Soed:Ertension by Crthodic Protcction Abstra* Plrclng secds on o n gotivelt charged conductor cxtended thcir vbbilIty dudng artificial aging. Such cathodic Prorcction may t.duc. lr.c rodicol anach by providing a tourcc ol electrons. Th. resuhs support th. hypolhesh ol lrec rdicaf datnaCc to cellular componen s snd orc cotlsiEtent wlth such damagc bcing l\tportanl ln d.tcriorotive tcncscencc changct. It is welt Lnown that frcc radicals ioitiate peroxidativc degradation of ulsaturated tissuc lipids, and it har bccn suggestedthat this could give risc to drmage to cellular membranes(1). Lipid peroxidatiotr il mooomolecular-an{ bimolecular ilms leads initially to au in-prease in membranc permeability and then to s decreas€in mcmbranc stability (2). Damage to biological membranes associated with lipid peroxidatioo has bee! shown in mitochondria (.r), microsomes (4), and lysosomes (J). Lipofuscin granules accumulate with chronological age in some animal lissues and these graDules app€ar to cootain protein and peroxidized lipid (6), iodicating that lipid peroxidation occurs throughout the lifc span of an organism. It has been suggcsted that this free radical tEroxidstiotr of uosaturated lipids is r basic deterioralive mechanismia cellularagiog (Z). There is as yet little direct €videocc for the accumulation of membraDe damag€ during senesceace.However, extremely swollen mitochondria, witb disorientated and fragmented cristae, indicative of menrbranedamage,havc beeo observed in the ffight muscle of old (25 to 30 days) male houseflies(E). Attempts have betn made to prolooS the life span of organisms,aod promising results havc beeo obtained by supplying membrane-stabilizinS drugs to firosophila (9) and by including various antioxidants in the diet of micc (7, ,10). Howcver. thcsc results are far from unequivocal: not all reatmetrts .re effective (7, 9), difierent resultsare obtaincd with th€ same rntioxidatrt on diffcrcnt strains of mic€ (7), and in I'" '0 DECEMEET, some exp€rimeDtslipid pcroxidationappearcd to bc teduccd without rDy cfiect on lifc spao (Ir). Moln6r (12) sttcmPtcdto rcducr frcc radical attack, Dot by supplyilg .!tioxi' dants itr the diet, but by maintairing rnice in a cagc with rn Epplicd ncgativc ttotentid it a maoDei ssaloSous fo tbe techniqoe of crthodic protcctiotr of metals agai$l corrosion. The average and maximum lifc apans of mice exposed to a negatiYc chargc were, rcspectivcly, 25 s'',d 32 pcrceot greater than those of micc cxposed to 8 positive charge. 9 € a 0 a y 3 o l a S L n gt r c a t m . n t Fig. l, viability of storcd lccds a'f z€a ,ralj a5 a functiotr of thc duration of thc aging t.ratmclL Scc& with a Doistutc contcnt of 13.6 pcrctnt wcrc placcd, embryo sidc do*n, o!| lluoirum foil ir a small cnclosed chambcr (to erintritr thc moisturc €ootcot) rDd tcltt !t /()'C to accel.rrte thcir rfi!8 (.c&cs thcir storagc lifG), Thc cntirp cotrtcdr of l singlc chambcrwcr! trtcn fc crh ranplc. Thc 6.cdr wcrc lootld ir trp rrtlr ovcrniSht and lhco plAcodo! Doid FrFr towcling. Germinatioo wrr r@rded t l boun rftcr th! ltrrt of !o*h& (r) Sccdt providcd vith cltbodic protlctioo by rF plyin! a n trtiw potctrtlsl ol 300 teltt to thc rlumitrum noil; (b) control !ccd& Air{ry rccdr c.! b. rrftdly rrrd rrtifci.lly by mriotriDitl tba r rclrtivcly hith mobtur! contctt (13 !o 15 Frc€ot) rnd rtr clcvtLd t6pctrturo (4OoC) (rr). Airldrt rccdr probrbly do not brc srficbrt D.&tura to pcrmit rqucoulphrac cozyrDo ltrctl)or to occur, aDd rcprb Gb|nL.or ur urlikely to b3 opcntivc ir !|rh a ryrtco. Thw, drmrt itrduc€d by ftG. rrdbr,lr would rccumul.tc io dry rGcdt, ?hd!'r in hydntcd liorx er6,t"-.lc canld bo rcpaircd (/a). Scodr of Zcr nry (ltdien com) thrt brvc boco artiicidty rgcd rbow rbcrrraioor of mitocboodrirl mcmbrrna s! ra arly dfp of rfiq. b€forc tb. viability ol thc tocds docliDca( It), Scc& of Zeo n.lt Ytra [bhclod to snifcid rtint tFrtmat by m8ir|irilf th€m in r rmdl cocloNcd chrltlboa |l /tooc- Tbc recdr wcro pmvidod vitl cathodic protcctioq by plrcint tbm, crnbryo ridc down, or tluminum foil -7 within tb chembcr ard rpptyiq r Dctativc potcotid of 300 volt! to tbc I foil. Control !.€dr r.rc mdtrtritad ur") dcr similar cooditioo. ritlout tbc rI> plicd cbrrgc. Tt cficcr of rtorflr timc on thc virbility of tbc rccds ir 3how! ir Fi& 1. Conridcrabl€ dccrclcr ir virbi.lity loss werc achicvcd wit! c.abodic ptotectioo. In rddition, tbe cooductivity of the wrtcr in whicb tbc lccdr rwrc soakcd bcforc grrminrtion wrt coosistcotly grcatcr for thc cootrol rccds than for tbc sccds which had bccn cxpos€d to thc ocgativc cbatgc. Tbir i!dicates that more matcrial was leachcd from the control rccds during soati4, which suggeststhat greater mcmbnoc damage had occurred in th€m th!! ir the seedsprovided with catbodic prot ction. Chromosome abcrrrtioor uc known to accumulatc in nondivkling c€lls of se€ds under cooditioor of EccleraGd agiot (rJ). Frcc rsdicd p.3oxidation may rfcct macromolocr cr other thao lipids; thu!, our obccnrtion that after l0 days of th agilg fratmeqt the perccotagc of chfomosomc ab€rrations wrs 12.6 in tbc contr,ol secds rDd 4.3 in tb€ protcct d i€cdr may bc of signifsaacc. Tbc rtductior| in mortality rEtc for dry rccdr, wbich arc relativcly nonhydrrtrd rtrtm, werc far trcrtcr thrn ray rcbbrrcd for animalr, rvhich rrc hydfrtcd rf&D" Crthgdic protoc-rbtr 6ould rodrn frec radicrl $bck or binlqicrl nrcm. molcculs Uy pmviOq r rourco d clectroB tO rrrct ?ith lbc frc ndk:llr (r2). Thuq tbd. re h, provids rtrola evi&G for frcc ndlrl durla 5 ltt r i ;. i,,l 1 l t !, ! llrl 1 ,i . .i I l l,lt it rl! l, t : 'I ii il' ii ti. ll , I l. rl l it [l rl ti 1 ii ll ll : I[ - CULTUR€ €L€CTRO This device employs natural electric and magnetic principles and costs nothing to op€rate. It will stimulate growtl over sn acre of ground. 't.rorcro.. 8rd. *'LLLLLLLL[!!f,!!!L|!|||| top vlar of oroaa oo top ot atat Dotr irr varttcrl Plp.a aaa lr coupll[3 .glt.. dlr. x {r lola t ld Dlp.r to rach otb.r t Id plpa crorr otr top ot coupltr Iav.l ,lla b.ro rtr. uoda!Bloutd Cl.ra J|ra lb.t ! rlr.. oqa lorlda T t -f rt .l , h h s ggiEigIF ttrll..t o.. to ttt tar ptp. .a.t looarl, gacord ot aart oDa on. ltr.ld. ol hr3.rt oD. i*[[sF ri i li ra+ r Iitar iI9IifIc* ; i i : * i ifl{ i* [ = gFF I ;i f iB: i€ t g 15 f * is; E; ig i+ iiiiiiii iF Fiig; g nfiEFFfi FliFEr niigiiPiisi* i5irsE i$irg; ii Fi i f+iiF igisEiF*f f iiiipgFFgiii 3ii;giF FitiiisEF r iiliriiii'il;ilEEl EF glrri +igirreit irr ;iiaigg FiiriirE Eg r a t { { rn T 7. e I t. ii'3ias 'iFs;s$litigirygm igguF iflEEBi s'iEstF , FilififiairiiF **iiii [Fi ilEti}iffi*iii ,sg' FEs iiii'i iil + giiuli i"ffiii FFu' ggrF igfFisi Ffi g;eii g Fiifff:ilrieEB* iF iiiFiei i >7 : o ! ' 6 6 ? . s3 a! 'o !? ;i .= -{ ' o o o 5 o i i ; i E F rt:gu F i i i i l i ; . l E ; + r e-z1:?i !i=t rfE irEa:rl i r : : i i . ; ? i i: i c g 3i: iiraiE;5r[; i ; F ; ; 9 g i = i: 4 E t f ri 1 1 i tl 1 ; ; a ; l f 'e;- A-ARa'6+ d-=.= ?,sti 3H lfr: a€iE iiiiiiigi*iE L - E? +.,i fD; :' 3: ;a' sl E €E : yt , ? E pE l; T5,;i " r ' r E i l . sf r ; r ;;iir+iiF :irg liiliilliii;iiililirl liiiil;lillriliii lir 1riilliiiiiiiIII*iliIlli1ii.f izt1li1III (r . o P aJ C r\O vt_ ! utlti t,i1::'1: i tiii iiiilll i ii:iiii I 8.5 ELEC1RIC FIELD EFFECTSON PLANT LIFE SeveraL Et.udies iLlustlate the use of relatively st!-..> __ efectric fields to affect the viability of seeds during storage and seed gernination, or to influerrce lhe growth of plints. - Panunenteret (1974) reported that stored seeds subjected 4, to an artificial aging process progressively lose the ability to germj.nate, so thaL after 30 days of treaiment only lOs of the seeds are viable. However, by placinq the seeds on an electrode at 300 volts Lhe germin;tlon ,.te aiter the 3o-dav aglng treatment lras inploved to about 7Ot. I'he data demonstra'Ce that a substantial impro!€ment in seed viability occurs for all degrees of aging and that the protected seeds a-l.so show a lower Percentage of ChromosOmal abnolnaLities. TheSe effectS are attlibuted to a form of "cathodic protection', in that the elei_ trode piovides electrons to react wj.th the free radical.s and thus inhibit the normal course of lipid peroxidation by which, i r i c har ior'-.1 rho ^al 1 menrDrane ls degtroyed in both arti_ fici.al and naLural aging processeg. sidaway (196g) examined the germination of seeals in an electrostatic fleld of 36 kv/h and cane to Lhe conclusion that a pole above the seeds inhibits field wi.th ehe positive gerrnination and,to a lesser extent, the negative pole above iiproves the rate of germination. I'lurr (1954; 1955) studled the destluctive effects r{h1ch grown in fields occur in plants of 20 - 80 kv/n (DC) createil abgve the groLrnd in h,hich Che planrs were by a plaee located The Leafy tips rooted. of grass planr6 were iniuied or killed stlong electrostatic field, which was i;tensified to - by-this field values at the leaf tip. prom his r n r J c hh l g h e r s u ! f a c e Mulr finds a surface field studies, of fIo - 13b kv/n ia about the threshol.d fo! destructive effects in these plants. !lu!r (1954) speculates that the mechanlsm by which tie planca are involves injured an acceleration of enzyme activity leading to inpaired cefl lesplration and further destruccive ivenr.s. lteehani srn of Natrrre_2Ol (4926):1305-6 Plant-Cel l l:J0 l)\'( ( S. ,\. \l . 'r l \l.l( lr Slll't.1 2. .li.rrr1,r. . 1 . .\rirtnrr Stlt( [ |irer,ir1, .\ surtr! ,,i lt,. (,r sr.d gcrIlrllali{nr clTccts ,ri rraxfl(lic trvirornrcr[' gro*rrr.p{a..t pl"6:"1g,1 andearry 11:4 Seed of t\crrtl (lifffrf'rl :l,eritr r,i Ilarrts cdrnnrorrtr rrr lal r:rt,,rr u$l ,rr,,rrI ir. rrr.rr.,,.r: t r r i r o r r r r r r r r r sr : r r r g i r g i r o r l a n u l l ( ( t r . H , r , l ( r r r - r c t i \ . irur.I u[ 0 to 2i nLilligauss ri] :r t)lrrr iir(,rr rrirsrjtl (,i -l'hc ootD gauss strrrgth. rnajorirl-,,i tlrr rfed gfrnri rratcd fastrr an(l t,r(!locc(l largcr r,,1r, i r(1 r(!)1. ailtr i\1' Beeks i,r tlrt rrasrrrticquiet rrgi,rr.-tlrr cu!l]lnb(r, anrl ratlish plants nrairrtaincil rhi\ incrrasetl ratc ()i pru$tlr i,,r th( duratio'r uf llrr trl'qrrrrr, r S , , r n c s c ( d s l r , N r r l a I a s t c r g e r r l l i r r a r i , r rr : r r r i , r 1 l r ( gfoD Ls1leli( lielrl arrd rrr.Lirrrainulrtrc acc(lcral.(l r:11( tlrr,,ughout thc extrri lc l. T,,ro rrerks airr.r ctrrllir:rtr) . (()rr, l,;rflrt ll|(l ver,lr, rrlrrlr' ,r;,fli A \t,,$rr r r l l r c { { r ) l l r : r a r r r i ( r l c l , l . . 1 r, r r r l : ' r j r ; , r k . , l j | . r ( 1 \ ( , i r r t c ! , i s r u $ l h , 1 s c ( r r l , r r ( ( l t , , l , l a n t s i r ) l l r r r j L r t l c ,tli ( t ( l -Sccrts placc,l ;I tllc \tror! llrrl.lr).rr. trrt(lj !e (.rirll\ $ere slower to Fljnllinatc ar){l di(l nl)l r :rir)raiu tlrr r]tr o i g r o \ t h s h o $ r t t ) y t h c I ' l a | r t r i r r t l r t g t r ) t 1 u r g n c r i ca t l r r u l l r d l i e k l s . C c r n r i n a t i , ' r r i r t l r e s r r o r s r r r a g n r : r i cl i e I l s was inRuenced by the lr,ririon ,ri rht axis oi the ,re(l rclativt t,) thc lirrt. oi n,rcc (,i rtrr: hcl,l. \\'htn rhe lllrts a - \ N o l s . r D t o t r l r c s r r ( l \ a . l r l a c t r li r r r r r h t a r r A l r : 1 o t l r t l i r r < s , , i i o r c e t h e r a t t o i g r o r r d r r a s n r u c r ls r o \ ! c : than th., rate ior thc srcrl plactd \ith lhc loll$ a\i. t , r r a l l . l t , r t h ( l i r r r s o i r , ; r c r . I l , j $ ! v ( r , C , , t c u sc u r t i r j r . r s . l r , $ ( d : r , r l : r r k L , 1i n t r u r . t i r r r ( r , r L l r , , \ r r r n r r : r ! n c u . r , . l 1 l o i l ( [ ) 0 8 : l | r s si t r c A l ] r . l l r r : r r l l I l r i r c r i r o o r . a r r r l tl)r lrrjjlh oi thc rr,ots \err hrth gfrar(r i ll). rr:r!: frir r(ld thilr on i rilar cutti g! ilr rhl] r)1,Ic(l 6elLl ,,r rhe 'lherc rronraHtlctic ticlil. \.as () :rt)trar(nt e,Trcr (nr rhc rutJsro\th oi the Colrus cutr; g\. 7 J (19?4) exposed the roots of the mung . w. MilleE bean plant (Vicia faba) Co electrj-c o! magnetic fields at 75 Hz wlcn no indfEa-ti6i*ot an effecC on eitier glowth or.itoti.-i.,a.*. No chtomosonal anomalies were seen. The electri.c f i.e1d exposure was made in a plastic tank frlled with a plant nutrient solution in which there was a current of 0.I A/m2 and an el-ectric field ^F 1n lrlh fha ^^^+/^1 ^l F -ants were in a similar tank for which the electrodes \rere not ene!9ized. The magnetic field experiments wele conducted at 0.5, 5, and L7 G in plastic cvlind;rs in a liquid containing the plants nutrient meaiurn ani Hlapped with electricat wire. The authors cite other "wel l-documin ted. (Beischer, research .1955; Audus and i{hish, l96dr Dunlop and SchmidC, 1969; Merrcle et al., L964) at higher fiel.d srrengths (fron several hundred t6 sEveraL thousand qauss) r,hlch did find effects on plant glowth and the mitotic index, buE jn viev of the I t l r R F , I . , .E . : RE € L € C T R O.C U LTU (I'far. 28, I964) Damaae i n an Electeostatlc Field Dlectrj.cal of plant arovtth proaluces lncreased st.inulation c^n.enl.rations of n'lnor elenents (Fe, Zn, \l ) in active leaf tips. Cnmpared to controls, such increases are of t,he order of several hundred per cent. This is due to an i n me t a b o 1 I c l e n z I ' r ne s i n t h e e t c t r i c a l l l ' - a c t i v a t e d .lncrerse ol i go 'pl rnts, whlch appear greener beeause ozone generated by electrI cal fle{d nridizes porphyr in s, re sul t ing in deep-green, open-r l n s e d m o ' le c u l e s o f t h e s e o r s i m l . l a r m e t a l l o - e n z . t ' m e s . U n d e r tho l-nfl uence of an excessive ele trical field, the actlvlty nf these substances is accelerated to such an extent that ccllular re spiration ls irFpedeal, wlth resul taql cel l deterlorat{ on t l s s u e and destructlon I I