Enzyme Inhibition Modes and Linearization Table
Mode of Inhibition →
Effect of inhibition on vmax
Effect of inhibition on K M
Competitive
No change
vmax
vmax
Increases
KM
Noncompetitive
Uncompetitive
[I ]
KI
KM 1
Plot depicting
uninhibited
enzyme and
increasing
concentrations
of inhibitor
slope =
Lineweaver-Burk
Linearization
How does
inhibitor alter
slope?
Reason?
How does
inhibitor alter
y-intercept?
Reason?
Eadie-Hofstee
Linearization
Hanes-Woolf
Linearization
KM
vmax
Inhibition causes
slope to increase
(since it increases
K M and leaves vmax
unchanged
1
y-intercept =
vmax
Inhibition leaves yintercept
unchanged since
vmax is unchanged
by inhibition
Plot depicting
uninhibited
enzyme and
increasing
concentrations
of inhibitor
How does
inhibitor alter
slope?
Reason?
How does
inhibitor alter
y-intercept?
Reason?
Plot depicting
uninhibited
enzyme and
increasing
concentrations
of inhibitor
Ganesh Sriram, BIOE120 Biology for Engineers, Spring 2022
1
Copyrighted Material; Only for Registered Students
Enzyme Inhibition Modes and Linearization Table
How does
inhibitor alter
slope?
Reason?
How does
inhibitor alter
y-intercept?
Reason?
Ganesh Sriram, BIOE120 Biology for Engineers, Spring 2022
2
Copyrighted Material; Only for Registered Students