Exercise 10: Primary Growth of Stem Introduction Stems play an integral part in the growth of plants, acting as the link between the roots and the rest of the plant 2 Functions of the Stem Supports the Leaves Conducts Water and Dissolved Mineral Salts Upward 3 Functions of the Stem Transport Food Materials Downwards Vegetative Reproduction and Storage 4 Objective 1. Identify the external and internal parts of the primary tissues of the stem 5 Objective 2. Differentiate the monocot and dicot stem and the herbaceous and young woody stems 6 Objective 3. Relate the parts of the stems with the functions 7 Materials Equipment, Plant Specimens, and Prepared Slides Used in the Laboratory Activity 8 Equipment Microscope 9 Plant Specimens Bambusa (Bamboo) Coleus (Mayana) Hibiscus (Gumamela) Nerium (Adelfa) 10 Prepared Slides Coleus or Elodea Shoot Tip Zea mays (corn) Commelina (alikbangon) Helianthus sp. Ranunculus sp. Tilia sp. Hibiscus sp. Aristolochia sp 11 Guide Questions EXTERNAL PARTS OF THE STEM 12 External Parts of the Stem Monocot Herbaceous Dicot Woody Dicot 13 External Parts of the Stem Monocot Herbaceous Dicot Woody Dicot 14 External Parts of the Stem Bases of Comparison Coleus Nerium or Hibiscus Bamboo Branches present present present Nodes present present present Internodes present present present Lenticels absent present absent Leaf Scars present present present Terminal Buds present present present Axillary Buds present present present green brown green smooth and supple to touch rough/bumpy smooth Color Texture 15 ‘’ Can you differentiate a monocot plant from a dicot plant just by looking at the stem? How? One main feature are the lenticels present only in mature dicot stems. 16 ‘’ What advantage would herbaceous stems have over woody stems? Herbaceous stems grow faster and have photosynthetic stems. 17 ‘’ What advantage would woody stems have over herbaceous stems? Woody stems counteract gravity so they can grow taller than herbaceous plants. 18 ‘’ What important functions do nodes have in a plant? Nodes contain the buds and leaves. 19 ‘’ What is the function of the lenticels? Do all stems have lenticels? Lenticels function for gas exchange. Not all stems have lenticels as only mature dicot stems have lenticels. 20 ‘’ What develops at the axils of leaves? Bud primordium develops at the axils of leaves. 21 ‘’ Are the internodes of the plants you examined of the same length? Give possible reasons for the similarities or differences in internode lengths among the plants that you examined. 22 Guide Questions SHOOT TIP 23 II. The Shoot Tip ▣ Coleus and Elodea shoot tip 24 ‘’ Where would you find older leaves on a stem, near the tip or near the lower part? They are near the lower parts of the stem. 25 ‘’ Why are the tips of some plants usually used as vegetables (e.g. kamote tops, kangkong tops, etc.)? Why are the lower parts not edible? The newer parts are edible while the lower parts are not because the lower parts are older. 26 ‘’ What feature in the terminal bud make it the most active part of the stem? It is mitotic with its meristems that continually produce leaves as necessary 27 Guide Questions PRIMARY TISSUES AND INTERNAL STRUCTURE OF THE STEM 28 Primary Tissues and Internal Structures Feature Vascular bundle arrangement Vascular cambium Monocot Vascular bundles are scattered Herbaceous Dicot Vascular bundles tapered to the center Young Woody Dicot Vascular bundles arranged in a ring Absent Present Present Specific supporting structures or tissues Outer Cortex (Collenchymal) Outer Cortex (Collenchymal) Outer Cortex (Collenchymal) Filler tissues Ground Tissue Ground Tissue Ground Tissue Parenchymal Tissue Parenchymal Tissue Parenchymal Tissue Storage tissues 29 Primary Tissues and Internal Structures Epidermis Ground Tissue Vascular Bundle Bundle Sheath Xylem Phloem 30 Primary Tissues and Internal Structures ▣ Monocot Stem (Zea mays) 31 Primary Tissues and Internal Structures Epidermis Cortex Phloem Vascular Cambium Xylem Pith 32 Primary Tissues and Internal Structures ▣ Herbaceous Young Dicot Stem (Tilia sp. or Ranunculus sp.) 33 Primary Tissues and Internal Structures Bundle Cap Fibers Fascicular Cambium Pith Xylem Interfascicular cambium Phloem Cortex Epidermis 34 Primary Tissues and Internal Structures ▣ Herbaceous Young Dicot Stem (Aristolochia sp.) 35 Guide Questions TRICHOMES 36 Trichomes ▣ Glandular 37 Trichomes ▣ Scale 38 Trichomes ▣ Stellate 39 Trichomes ▣ Bristle 40 Trichomes ▣ Peltate 41 Trichomes ▣ Branched 42 Guide Questions MODIFIED STEMS 43 Modified Stems Cladophyll Tendril 44 Modified Stems Stolon Tuber 45 Modified Stems Bulb (Tunicate) Bulb (Scaly) 46 Modified Stems Rhizome Corm 47 ‘’ What makes herbaceous plants more valuable to people as a food source than woody plants? Herbaceous plants function better as a food source since woody plants are heavily lignified. 48 ‘’ Are axillary buds also present in monocots? Give proof of your answer. Yes as monocots also produce lateral shoots or branches which is proof of axillary buds. 49 ‘’ Is the formation of leaf primordia and stem tissues orderly or random? They are orderly. 50 Thanks! Any questions? 51