PHOTOSYNTHESIS & CELLULAR RESPIRATION Definitions • Photosynthesis – How a Plant (Autotroph) Harnesses Light Energy to Make Chemical Energy • Respiration – Turning Chemical Energy into Fuel for Growth, Development & Reproduction Autotrophs – produce their own food like plants. What is Photosynthesis? The process of photosynthesis is a chemical reaction. It is the most important chemical reaction on our planet. Photosynthesis Described: • The process of changing light energy to chemical energy • The Chemical Energy is stored as sugar • Occurs in AUTOTROPHS: plants & some algae • Plants need light energy, CO2, and H2O • Takes place in the chloroplasts, using chlorophyll, the green pigment in plants Chloroplasts • Organelles that are only found in a Plant Cell • Location of Photosynthesis http://www.lclark.edu/~seavey/Bio100_03/Lecture%20notes/lecture_Feb_11.h What do plants need for photosynthesis? •Water •Carbon dioxide •Light •chlorophyll PHOTOSYNTHESIS EQUATION: 6 CO2 + 6 H2O + light C6H12O6 + 6 O2 ENERGY IN A CELL!! • Your body requires energy in order to function…from your heart beating to intense workouts. • Energy is essential to LIFE!! • Your body stores energy in a molecule called ATP (adenosine triphosphate) • This is a quick source of usable energy for any organelle in the cell that needs it. The energy inside an ATP molecule is released when it loses a phosphate and becomes ADP (adenosine diphosphate) triphosphate diphosphate 3 x PO4 2 x PO4 ADP is like a partially charged battery while ATP is a like a fully charged battery. PHOTOSYNTHESIS Photosynthesis occurs in organelles, present in green plants and certain algae, called Chloroplasts. Chloroplast Chloroplast These chloroplasts contain the green pigment called chlorophyll. “Chlorophyll, more like borophyll!” • https://youtu.be/HrNse yswKak • (1:38) Chloroplast Structure • Inner membrane called the thylakoid membrane. • Thickened regions called thylakoids. A stack of thylakoids is called a granum. (Plural – grana) • Stroma is a liquid surrounding the thylakoids. STOMATA STOMATA Stomata are important for the exchange of gases (CO2 & O2) by diffusion between the outside air and intercellular spaces for respiration (and also for the evaporation of water by transpiration. ) https://youtu.be/pFaBp VoQD4E (5 min) PHOTOSYNTHESIS EQUATION: 6 CO2 + 6 H2O + light C6H12O6 + 6 O2 CHLOROPHYLL • Chlorophyll- found in plants • will absorb red, blue, and violet better than other colors. • Green light is primarily reflected so most leaves appear green to us. CHLOROPHYLL Two Types of Chlorophyll – each absorb light differently… Chlorophyll a absorbs light mainly in blue-violet & red regions. Chlorphyll b absorbs light in the blue and red regions more. CHLOROPHYLL • Because light is a form of energy, any compound that absorbs light also absorbs the energy in that light. • When chlorophyll absorbs light much of the energy is transferred directly to electrons in the chlorophyll molecule, raising the energy available in these electrons. CHLOROPHYLL • Electron carriers are seen throughout Photosynthesis. • They are compounds that can accept high-energy electrons and transfer them along with most of their energy to another molecule. Examples… – NADP+ – ADP NADPH ATP Breaking off the Phosphate is what releases energy from these molecules: ATP ADP + Phosphate + Energy NADPH NADH + Phosphate + Energy 2 REACTIONS OF PHOTOSYNTHESIS Photosynthesis involves 2 reactions: 1. Light Reaction makes O2 2. Calvin Cycle makes C6H12O6 (Also called Light Independent Reaction and Dark Reaction.) Light-dependent Reactions • Electron transport system: sequence of electron carrier molecules that shuttle electrons, energy released to make ATP • Oxygen is made from the light reactions • Light reactions make ATP and NADPH • used for Calvin cycle (dark reactions) Calvin Cycle (“dark” reactions) • ATP & NADPH generated in light reactions are used to fuel the reactions in the Calvin Cycle which take CO2 and break it apart, then reassemble the carbons into glucose. • Called carbon fixation: CO2 C6H12O6 Photosynthesis light reactions ATP & NADPH Calvin Cycle C6H12O6 PHOTOSYNTHESIS VIDEO ~7 MIN https://youtu.be/KfvYQgT2M-k PHOTOSYNTHESIS HW: pg 76 #1-5 in Text Quizlet posted. https://quizlet.com/_66rt1t PRACTICE: Khan Academy https://www.khanacademy.org/science/biology/photosynthesis-in-plants/introductionto-stages-of-photosynthesis/e/photosynthesis Photosynthecizing Animals… https://youtu.be/AcX2n1rC4W4 Notice how the equation for photosynthesis relates to the reactions shown in the diagram below. 6CO2 + 6H2O + Energy C6H12O6 + 6O2 CELLULAR RESPIRATION C6H12O6 + 6O2 6 CO2 + 6 H2O + Energy CELLULAR RESPIRATION A car and our bodies are similar in the fact that the mitochondria is like an engine that uses O2 to burn sugar as fuel. The exhaust is H2O & CO2 . CELLULAR RESPIRATION • Process by which living organisms obtain energy from the chemical bonds of food. • The two important ways cells can harvest energy from food… 1) cellular respiration & 2) fermentation (aerobic - O2) (anaerobic – No O2 ) lots of ATP! Less energy made AEROBIC RESPIRATION • Aerobic respiration occurs when oxygen is present. • Aerobic respiration produces large amounts of energy (ATP) ANAEROBIC RESPIRATION • Anaerobic respiration occurs when oxygen is NOT present. • Produces few ATP’s • Called fermentation. discussed last in the slides… WHAT IS ATP? Adenosine Triphosphate is a compound that contains - 3 high energy phosphate bonds & is an important source of energy for cells ATP CELLULAR RESPIRATION Cellular Respiration consists of 3 major stages: • Glycolysis (in the cytosol / cytoplasm) • Krebs Cycle (mitochondria) • Electron Transport Chain (mitochondria) (ETC for short) 2 2 34 1) GLYCOLYSIS • The 1st stage of cellular respiration. • Occurs in the cytoplasm of the cell. • Occurs in both aerobic & anaerobic conditions. • BUT Does NOT require oxygen GLYCOLSIS: • glucose turned to 2 molecules of pyruvate • Gives net gain of 2x molecules. GLYCOLYSIS: glucose 2 pyruvate molecules + 2 ATP molecules 2) KREBS CYCLE nd •2 phase of aerobic respiration • Occurs in the Mitochondria KREBS CYCLE The process of Kreb Cycle is essentially: - removing CO2 carbon dioxide - getting out energy in the form of ATP for energy, NADH & FADH (Electron Carriers) - & then regenerating the cycle. 2 KREBS CYCLE The process of Kreb Cycle is essentially: MAKES 2 MAKES CO2 s . MAKES NADH & FADH2 (Electron Carriers for energy for next step ETC ) 3) ELECTRON TRANSPORT CHAIN (ETC) • 3rd stage of cellular respiration • Reactions in which an electron is passed from one molecule to another to produce energy to make EVEN MORE ! 3) ELECTRON TRANSPORT CHAIN (ETC) • The electron carriers (NADH & FADH2 ) move electrons down the chain along the inner membrane of the mitochondria. To Give You an Idea… – not to be memorized 3) ELECTRON TRANSPORT CHAIN (ETC) • As hydrogen ions move across the channels in the membrane, they release energy that is used by an enzyme to make ATP • H2O Water is formed in this process ...it is a by-product 3) ELECTRON TRANSPORT CHAIN (ETC) • The Result the electron transport chain produces 32 molecules of • O2 enters the ETC & absorbs electrons • H from C6H12O6 & extra O2 make H2O is a by-product TOTAL - Cellular Respiration HOW MUCH is finally produced??? 2 x ATPs 1) Glycolysis 2) Krebs Cycle 2 x ATPs 3) Electron Transport Chain 32 x ATPs TOTAL = 36 s OVERVIEW • Glycolysis is anaerobic no oxygen • If oxygen is present then … Krebs cycle & ETC pathway is taken • If oxygen is NOT present, a different pathway is taken fermentation FERMENTATION - Anaerobic • No O2 used ! • Fermentation releases energy from food molecules in the absence of oxygen. Aerobic Anaerobic 2 2 34 The Process of Fermentation • In Glycolysis 2 pyruvate were made from glucose • Pyruvate is then broken down into: ethyl alcohol or lactic acid • Only results in 2 molecules ALCOHOLIC FERMENTATION • Most common type of fermentation is yeast cells converting pyruvate into ethyl alcohol • The process enables yeast cells to convert the sugar in dough to ethyl alcohol & carbon dioxide Glycolysis: Fermentation: FERMENTATION in Bread • As yeast ferments, it causes dough to rise & create the bubbles that are present in bread • An example of alcoholic fermentation of yeast • As the dough bakes, yeast cells die and the alcohol they made evaporates FERMENTATION Also used to make wine, beer, & ethanol added to gasoline. LACTIC ACID FERMENTATION • Occurs in muscle cells when they use up all the available oxygen Video clip: - 10 seconds https://youtu.be/uyM6Wyg0xPg • Muscle cells feel fatigued and sore b/c lactic acid forms when oxygen is NOT present • This goes away in a few hours. LACTIC ACID • Most lactic acid made in muscles diffuses into bloodstream, then to liver, where it is converted back to pyruvate, which enters the aerobic respiration process… Kreb’s Cycle & Electron Transport Chain LACTIC ACID Products Yogurt, cheeses, rye bread, pickles, sauerkraut, and olives are lactic acid products